Restriction map for pF1KE1589
 (Linear) (Six-base) MAPSORT of: KIEE1589.seq  Check: 676  from: 1  to: 3156



KIEE1589.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     275     952    3156 
   Size:        275     677    2204

  Fragments arranged by size: 

                2204     677     275
 
AccI GT'mk_AC

Cuts at:      0     749    3156 
   Size:        749    2407
 
AclI AA'CG_TT

Cuts at:      0      81    3156 
   Size:         81    3075
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    2536    2695    3156 
   Size:        434    2102     159     461

  Fragments arranged by size: 

                2102     461     434     159
 
AfeI AGC'GCT

Cuts at:      0    2470    3156 
   Size:       2470     686
 
AflIII A'CryG_T

Cuts at:      0    1363    3156 
   Size:       1363    1793
 
AgeI A'CCGG_T

Cuts at:      0    2753    3156 
   Size:       2753     403
 
AleI CACnn'nnGTG

Cuts at:      0     892    3156 
   Size:        892    2264
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     194    3156 
   Size:        194    2962
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2605    3156 
   Size:       2605     551
 
ApaLI G'TGCA_C

Cuts at:      0     965    1503    3156 
   Size:        965     538    1653

  Fragments arranged by size: 

                1653     965     538
 
ApoI r'AATT_y

Cuts at:      0      42     374     432     724    1399    3156 
   Size:         42     332      58     292     675    1757

  Fragments arranged by size: 

                1757     675     332     292      58      42
 
AvaI C'yCGr_G

Cuts at:      0     970    2519    3156 
   Size:        970    1549     637

  Fragments arranged by size: 

                1549     970     637
 
BanI G'GyrC_C

Cuts at:      0     990    3156 
   Size:        990    2166
 
BanII G_rGCy'C

Cuts at:      0    2643    2990    3156 
   Size:       2643     347     166

  Fragments arranged by size: 

                2643     347     166
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     929    2094    2542    3156 
   Size:        929    1165     448     614

  Fragments arranged by size: 

                1165     929     614     448
 
BbvCI CC'TCA_GC

Cuts at:      0    1519    1800    2671    3156 
   Size:       1519     281     871     485

  Fragments arranged by size: 

                1519     871     485     281
 
BclI T'GATC_A

Cuts at:      0     480    3156 
   Size:        480    2676
 
BfrBI ATG'CAT

Cuts at:      0    1851    3156 
   Size:       1851    1305
 
BlpI GC'TnA_GC

Cuts at:      0    2021    2026    2054    3156 
   Size:       2021       5      28    1102

  Fragments arranged by size: 

                2021    1102      28       5
 
Bme1580I G_kGCm'C

Cuts at:      0     969    1507    3156 
   Size:        969     538    1649

  Fragments arranged by size: 

                1649     969     538
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     549    2156    3156 
   Size:        549    1607    1000

  Fragments arranged by size: 

                1607    1000     549
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2534    3156 
   Size:       2534     622
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     728    1754    2059    2657    3019    3156 
   Size:        297     431    1026     305     598     362     137

  Fragments arranged by size: 

                1026     598     431     362     305     297     137
 
Bpu10I CC'TnA_GC

Cuts at:      0    1519    1800    2671    3156 
   Size:       1519     281     871     485

  Fragments arranged by size: 

                1519     871     485     281
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     875    3156 
   Size:        875    2281
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2902    3141    3156 
   Size:       2902     239      15

  Fragments arranged by size: 

                2902     239      15
 
BsaAI yAC'GTr

Cuts at:      0     297    3156 
   Size:        297    2859
 
BsaBI GATnn'nnATC

Cuts at:      0    1307    3156 
   Size:       1307    1849
 
BsaHI Gr'CG_yC

Cuts at:      0     308    3156 
   Size:        308    2848
 
BsaWI w'CCGG_w

Cuts at:      0    2113    2753    3156 
   Size:       2113     640     403

  Fragments arranged by size: 

                2113     640     403
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     292     367    2857    3156 
   Size:        292      75    2490     299

  Fragments arranged by size: 

                2490     299     292      75
 
BseYI C'CCAG_C

Cuts at:      0    1973    1987    2143    2191    3011    3156 
   Size:       1973      14     156      48     820     145

  Fragments arranged by size: 

                1973     820     156     145      48      14
 
BsiEI CG_ry'CG

Cuts at:      0    1899    2758    3156 
   Size:       1899     859     398

  Fragments arranged by size: 

                1899     859     398
 
BsiHKAI G_wGCw'C

Cuts at:      0     783     969    1507    2643    2990    3156 
   Size:        783     186     538    1136     347     166

  Fragments arranged by size: 

                1136     783     538     347     186     166
 
BsmI GAATG_Cn'

Cuts at:      0     772     947    3156 
   Size:        772     175    2209

  Fragments arranged by size: 

                2209     772     175
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     315    1170    3156 
   Size:        315     855    1986

  Fragments arranged by size: 

                1986     855     315
 
Bsp1286I G_dGCh'C

Cuts at:      0     783     969    1507    2643    2990    3156 
   Size:        783     186     538    1136     347     166

  Fragments arranged by size: 

                1136     783     538     347     186     166
 
BspEI T'CCGG_A

Cuts at:      0    2113    3156 
   Size:       2113    1043
 
BspHI T'CATG_A

Cuts at:      0    3023    3156 
   Size:       3023     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     275     952    2393    3156 
   Size:        275     677    1441     763

  Fragments arranged by size: 

                1441     763     677     275
 
BsrDI GCAATG_nn'

Cuts at:      0     101     561    1004    1637    2351    3156 
   Size:        101     460     443     633     714     805

  Fragments arranged by size: 

                 805     714     633     460     443     101
 
BsrFI r'CCGG_y

Cuts at:      0    1043    2753    3156 
   Size:       1043    1710     403

  Fragments arranged by size: 

                1710    1043     403
 
BsrGI T'GTAC_A

Cuts at:      0    1305    1626    3156 
   Size:       1305     321    1530

  Fragments arranged by size: 

                1530    1305     321
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1149    2406    3156 
   Size:       1149    1257     750

  Fragments arranged by size: 

                1257    1149     750
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     152     356    2233    3156 
   Size:        152     204    1877     923

  Fragments arranged by size: 

                1877     923     204     152
 
BstYI r'GATC_y

Cuts at:      0     734    1554    2601    3156 
   Size:        734     820    1047     555

  Fragments arranged by size: 

                1047     820     734     555
 
Bsu36I CC'TnA_GG

Cuts at:      0    2333    2881    3156 
   Size:       2333     548     275

  Fragments arranged by size: 

                2333     548     275
 
BtgI C'CryG_G

Cuts at:      0    2264    3156 
   Size:       2264     892
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     858     893    3156 
   Size:        858      35    2263

  Fragments arranged by size: 

                2263     858      35
 
DraI TTT'AAA

Cuts at:      0     608    3156 
   Size:        608    2548
 
EaeI y'GGCC_r

Cuts at:      0    1127    2894    3156 
   Size:       1127    1767     262

  Fragments arranged by size: 

                1767    1127     262
 
EarI CTCTTCn'nnn_

Cuts at:      0     448    2039    2057    2420    3156 
   Size:        448    1591      18     363     736

  Fragments arranged by size: 

                1591     736     448     363      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     244    3156 
   Size:        244    2912
 
EcoICRI GAG'CTC

Cuts at:      0    2641    2988    3156 
   Size:       2641     347     168

  Fragments arranged by size: 

                2641     347     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     434     728    1754    2059    2536    2657    2695 
   Size:        297     137     294    1026     305     477     121      38

Cuts at:   2695    3019    3156 
   Size:        324     137

  Fragments arranged by size: 

                1026     477     324     305     297     294     137     137
                 121      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1233    1868    3156 
   Size:       1233     635    1288

  Fragments arranged by size: 

                1288    1233     635
 
EcoRI G'AATT_C

Cuts at:      0     374    3156 
   Size:        374    2782
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     786    3156 
   Size:        786    2370
 
HaeII r_GCGC'y

Cuts at:      0    2472    3156 
   Size:       2472     684
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     242     274     465     497     724     726     756     758 
   Size:        242      32     191      32     227       2      30       2

Cuts at:    758    1507    1539    1546    1578    3054    3086    3156 
   Size:        749      32       7      32    1476      32      70

  Fragments arranged by size: 

                1476     749     242     227     191      70      32      32
                  32      32      32      30       7       2       2
 
HindIII A'AGCT_T

Cuts at:      0    1733    2707    3156 
   Size:       1733     974     449

  Fragments arranged by size: 

                1733     974     449
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     696    2463    3156 
   Size:        696    1767     693

  Fragments arranged by size: 

                1767     696     693
 
MscI TGG'CCA

Cuts at:      0    1129    2896    3156 
   Size:       1129    1767     260

  Fragments arranged by size: 

                1767    1129     260
 
MslI CAynn'nnrTG

Cuts at:      0     176     892    1321    2074    2263    2809    3156 
   Size:        176     716     429     753     189     546     347

  Fragments arranged by size: 

                 753     716     546     429     347     189     176
 
MspA1I CmG'CkG

Cuts at:      0       7    3156 
   Size:          7    3149
 
NaeI GCC'GGC

Cuts at:      0    1045    3156 
   Size:       1045    2111
 
NcoI C'CATG_G

Cuts at:      0    2264    3156 
   Size:       2264     892
 
NgoMIV G'CCGG_C

Cuts at:      0    1043    3156 
   Size:       1043    2113
 
NsiI A_TGCA'T

Cuts at:      0    1853    3156 
   Size:       1853    1303
 
NspI r_CATG'y

Cuts at:      0     238    1152    1367    3156 
   Size:        238     914     215    1789

  Fragments arranged by size: 

                1789     914     238     215
 
PciI A'CATG_T

Cuts at:      0    1363    3156 
   Size:       1363    1793
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2421    3156 
   Size:       2421     735
 
PfoI T'CCnGG_A

Cuts at:      0    2036    3156 
   Size:       2036    1120
 
PmlI CAC'GTG

Cuts at:      0     297    3156 
   Size:        297    2859
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      66      98    3156 
   Size:         66      32    3058

  Fragments arranged by size: 

                3058      66      32
 
PstI C_TGCA'G

Cuts at:      0    2610    3156 
   Size:       2610     546
 
PvuII CAG'CTG

Cuts at:      0       7    3156 
   Size:          7    3149
 
SacI G_AGCT'C

Cuts at:      0    2643    2990    3156 
   Size:       2643     347     166

  Fragments arranged by size: 

                2643     347     166
 
SapI GCTCTTCn'nnn_

Cuts at:      0     448    2057    2420    3156 
   Size:        448    1609     363     736

  Fragments arranged by size: 

                1609     736     448     363
 
ScaI AGT'ACT

Cuts at:      0      51    1722    3156 
   Size:         51    1671    1434

  Fragments arranged by size: 

                1671    1434      51
 
SexAI A'CCwGG_T

Cuts at:      0    2225    3156 
   Size:       2225     931
 
SfcI C'TryA_G

Cuts at:      0     750    1953    2372    2606    3156 
   Size:        750    1203     419     234     550

  Fragments arranged by size: 

                1203     750     550     419     234
 
SmlI C'TyrA_G

Cuts at:      0     890    2519    3156 
   Size:        890    1629     637

  Fragments arranged by size: 

                1629     890     637
 
SphI G_CATG'C

Cuts at:      0    1152    3156 
   Size:       1152    2004
 
StyI C'CwwG_G

Cuts at:      0    2264    3156 
   Size:       2264     892
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     359    1885    2950    2971    3156 
   Size:        359    1526    1065      21     185

  Fragments arranged by size: 

                1526    1065     359     185      21
 
TatI w'GTAC_w

Cuts at:      0      49    1305    1375    1591    1626    1720    3044    3156 
   Size:         49    1256      70     216      35      94    1324     112

  Fragments arranged by size: 

                1324    1256     216     112      94      70      49      35
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1461    1493    1809    1841    3156 
   Size:       1461      32     316      32    1315

  Fragments arranged by size: 

                1461    1315     316      32      32
 
XbaI T'CTAG_A

Cuts at:      0     519    1608    3156 
   Size:        519    1089    1548

  Fragments arranged by size: 

                1548    1089     519
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     353    3156 
   Size:        353    2803
 
XhoI C'TCGA_G

Cuts at:      0    2519    3156 
   Size:       2519     637
 
XmnI GAAnn'nnTTC

Cuts at:      0     436     716    2492    3156 
   Size:        436     280    1776     664

  Fragments arranged by size: 

                1776     664     436     280
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AvaI     BanI    BanII     BbsI
    BbvCI     BclI    BfrBI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    CspCI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI     FalI    HaeII    Hin4I  HindIII     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI     PciI
    PflMI     PfoI     PmlI     PsrI     PstI    PvuII     SacI     SapI
     ScaI    SexAI     SfcI     SmlI     SphI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII     AhdI     AloI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BglI
    BglII    BmgBI     BmtI    BsaXI     BsgI    BsiWI    BsrBI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI   DraIII
     DrdI     EagI EcoO109I    EcoRV     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     PacI     PasI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XmaI     ZraI
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