Restriction map for pF1KB6538
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BsiEI       BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MseI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        113  TCCGGA               T^CCGGA
AcsI          661  GAATTC               [GA]^AATT[CT]
AhdI          382  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          222  GATCC                GGATC(4/5)
ApaI         1131  GGGCCC               GGGCC^C
ApoI          661  GAATTC               [GA]^AATT[CT]
Asp700I       231  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         382  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         533  TGCGCA               TGC^GCA
BbvCI         853  CCTCAGC              CCTCAGC(-5/-2)
BlpI         1039  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1039  GCTGAGC              GC^T[ACGT]AGC
BsaWI         113  TCCGGA               [AT]^CCGG[AT]
BseAI         113  TCCGGA               T^CCGGA
BsgI          482  CTGCAC               GTGCAG(16/14)
BsmI          710  GCATTC               GAATGC(1/-1)
BspEI         113  TCCGGA               T^CCGGA
BspMI         206  ACCTGC               ACCTGC(4/8)
BstXI         569  CCAAAGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         221  AGATCC               [GA]^GATC[CT]
CelII        1039  GCTGAGC              GC^T[ACGT]AGC
Eam1105I      382  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          166  CTCTTC               CTCTTC(1/4)
EcoNI         594  CCTTGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         661  GAATTC               G^AATTC
FspI          533  TGCGCA               TGC^GCA
Ksp632I       166  CTCTTC               CTCTTC(1/4)
MaeII          26  ACGT                 A^CGT
MflI          221  AGATCC               [GA]^GATC[CT]
MroI          113  TCCGGA               T^CCGGA
MslI          715  CATTTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          428  ACATGC               [GA]CATG^[CT]
PflMI         816  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI           76  GAGCTC               GAGCT^C
SapI          165  GCTCTTC              GCTCTTC(1/4)
SbfI          393  CCTGCAGG             CCTGCA^GG
SmlI          655  CTCAAG               C^T[CT][GA]AG
Sse8387I      393  CCTGCAGG             CCTGCA^GG
TaqI          407  TCGA                 T^CGA
TfiI          702  GAATC                G^A[AT]TC
Tsp509I       662  AATT                 ^AATT
TthHB8I       407  TCGA                 T^CGA
Van91I        816  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1136  CCAGCCCTGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         221  AGATCC               [GA]^GATC[CT]
XmnI          231  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         436  CGCG                 CG^CG
              956  CGCG                
AfaI         1049  GTAC                 GT^AC
             1117  GTAC                
AspHI          34  GTGCTC               G[AT]GC[AT]^C
               76  GAGCTC              
BalI          638  TGGCCA               TGG^CCA
              785  TGGCCA              
BsiHKAI        34  GTGCTC               G[AT]GC[AT]^C
               76  GAGCTC              
BsrDI         337  CATTGC               GCAATG(2/0)
              944  GCAATG              
BssSI         544  CACGAG               CACGAG(-5/-1)
              417  CTCGTG              
BstF5I        528  CATCC                GGATG(2/0)
             1104  CATCC               
BstUI         436  CGCG                 CG^CG
              956  CGCG                
DpnII         222  GATC                 ^GATC
              921  GATC                
FokI          528  CATCC                GGATG(9/13)
             1104  CATCC               
HgaI          498  GACGC                GACGC(5/10)
              837  GCGTC               
MboI          222  GATC                 ^GATC
              921  GATC                
MluNI         638  TGGCCA               TGG^CCA
              785  TGGCCA              
MscI          638  TGGCCA               TGG^CCA
              785  TGGCCA              
MspA1I        331  CAGCTG               C[AC]G^C[GT]G
              616  CAGCTG              
MvnI          436  CGCG                 CG^CG
              956  CGCG                
NcoI          575  CCATGG               C^CATGG
              926  CCATGG              
NdeII         222  GATC                 ^GATC
              921  GATC                
PpuMI         203  AGGACCT              [GA]G^G[AT]CC[CT]
              818  AGGACCT             
PstI          394  CTGCAG               CTGCA^G
              619  CTGCAG              
PvuII         331  CAGCTG               CAG^CTG
              616  CAGCTG              
RsaI         1049  GTAC                 GT^AC
             1117  GTAC                
Sau3AI        222  GATC                 ^GATC
              921  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrBI       BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboII       MnlI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PleI        Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Tsp45I      TspRI       
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