Restriction map for pF1KE3557
 (Linear) (Six-base) MAPSORT of: KIEE3557.seq  Check: 8740  from: 1  to: 1644



KIEE3557.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AatII G_ACGT'C

Cuts at:      0    1268    1644 
   Size:       1268     376
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1309    1644 
   Size:       1309     335
 
AfeI AGC'GCT

Cuts at:      0     880    1644 
   Size:        880     764
 
AhdI GACnn_n'nnGTC

Cuts at:      0     867    1644 
   Size:        867     777
 
AleI CACnn'nnGTG

Cuts at:      0     536    1644 
   Size:        536    1108
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     554    1519    1644 
   Size:        554     965     125

  Fragments arranged by size: 

                 965     554     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0     448     782     816    1026    1391    1644 
   Size:        448     334      34     210     365     253

  Fragments arranged by size: 

                 448     365     334     253     210      34
 
ApaI G_GGCC'C

Cuts at:      0     684     946    1532    1644 
   Size:        684     262     586     112

  Fragments arranged by size: 

                 684     586     262     112
 
AvaI C'yCGr_G

Cuts at:      0      52      67      88    1312    1546    1644 
   Size:         52      15      21    1224     234      98

  Fragments arranged by size: 

                1224     234      98      52      21      15
 
BanI G'GyrC_C

Cuts at:      0    1104    1644 
   Size:       1104     540
 
BanII G_rGCy'C

Cuts at:      0      19     115     128     684     946    1287    1319    1532 
   Size:         19      96      13     556     262     341      32     213

Cuts at:   1532    1607    1644 
   Size:         75      37

  Fragments arranged by size: 

                 556     341     262     213      96      75      37      32
                  19      13
 
BbvCI CC'TCA_GC

Cuts at:      0     658    1644 
   Size:        658     986
 
BglI GCCn_nnn'nGGC

Cuts at:      0     734     950    1141    1644 
   Size:        734     216     191     503

  Fragments arranged by size: 

                 734     503     216     191
 
Bme1580I G_kGCm'C

Cuts at:      0     684     946    1532    1644 
   Size:        684     262     586     112

  Fragments arranged by size: 

                 684     586     262     112
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     297     447    1644 
   Size:        297     150    1197

  Fragments arranged by size: 

                1197     297     150
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     362     555     577     736     976     985    1644 
   Size:        256     106     193      22     159     240       9     659

  Fragments arranged by size: 

                 659     256     240     193     159     106      22       9
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      13     658     800    1644 
   Size:         13     645     142     844

  Fragments arranged by size: 

                 844     645     142      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    1644 
   Size:        987     657
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1552    1644 
   Size:       1552      92
 
BsaHI Gr'CG_yC

Cuts at:      0     566    1265    1644 
   Size:        566     699     379

  Fragments arranged by size: 

                 699     566     379
 
BsaWI w'CCGG_w

Cuts at:      0     521    1279    1644 
   Size:        521     758     365

  Fragments arranged by size: 

                 758     521     365
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     270     397     853     919    1110    1644 
   Size:        106     164     127     456      66     191     534

  Fragments arranged by size: 

                 534     456     191     164     127     106      66
 
BseYI C'CCAG_C

Cuts at:      0     360     393     419    1256    1644 
   Size:        360      33      26     837     388

  Fragments arranged by size: 

                 837     388     360      33      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    1525    1644 
   Size:       1138     387     119

  Fragments arranged by size: 

                1138     387     119
 
BsiEI CG_ry'CG

Cuts at:      0     919    1644 
   Size:        919     725
 
BsiHKAI G_wGCw'C

Cuts at:      0     485    1481    1607    1644 
   Size:        485     996     126      37

  Fragments arranged by size: 

                 996     485     126      37
 
BsmI GAATG_Cn'

Cuts at:      0     927    1644 
   Size:        927     717
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      49    1644 
   Size:         49    1595
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     115     128     485     684     946    1287    1319 
   Size:         19      96      13     357     199     262     341      32

Cuts at:   1319    1481    1532    1607    1644 
   Size:        162      51      75      37

  Fragments arranged by size: 

                 357     341     262     199     162      96      75      51
                  37      32      19      13
 
BspEI T'CCGG_A

Cuts at:      0     521    1644 
   Size:        521    1123
 
BsrBI CCG'CTC

Cuts at:      0    1423    1644 
   Size:       1423     221
 
BsrDI GCAATG_nn'

Cuts at:      0     794    1349    1644 
   Size:        794     555     295

  Fragments arranged by size: 

                 794     555     295
 
BssHII G'CGCG_C

Cuts at:      0    1067    1644 
   Size:       1067     577
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1026    1644 
   Size:       1026     618
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     465    1578    1644 
   Size:        465    1113      66

  Fragments arranged by size: 

                1113     465      66
 
Bsu36I CC'TnA_GG

Cuts at:      0     613    1644 
   Size:        613    1031
 
BtgI C'CryG_G

Cuts at:      0      37     107     246     947    1146    1577    1644 
   Size:         37      70     139     701     199     431      67

  Fragments arranged by size: 

                 701     431     199     139      70      67      37
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     472    1644 
   Size:        472    1172
 
BtsI GCAGTG_nn'

Cuts at:      0     229    1015    1129    1624    1644 
   Size:        229     786     114     495      20

  Fragments arranged by size: 

                 786     495     229     114      20
 
EaeI y'GGCC_r

Cuts at:      0    1073    1190    1355    1514    1644 
   Size:       1073     117     165     159     130

  Fragments arranged by size: 

                1073     165     159     130     117
 
EarI CTCTTCn'nnn_

Cuts at:      0    1300    1327    1456    1550    1644 
   Size:       1300      27     129      94      94

  Fragments arranged by size: 

                1300     129      94      94      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1330    1644 
   Size:       1330     314
 
EcoICRI GAG'CTC

Cuts at:      0    1605    1644 
   Size:       1605      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     362     555     577     736     976     985    1309 
   Size:        256     106     193      22     159     240       9     324

Cuts at:   1309    1644 
   Size:        335

  Fragments arranged by size: 

                 335     324     256     240     193     159     106      22
                   9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     240     306     390     681     943    1644 
   Size:         33     207      66      84     291     262     701

  Fragments arranged by size: 

                 701     291     262     207      84      66      33
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     276    1314    1644 
   Size:        276    1038     330

  Fragments arranged by size: 

                1038     330     276
 
HaeII r_GCGC'y

Cuts at:      0     882    1236    1257    1644 
   Size:        882     354      21     387

  Fragments arranged by size: 

                 882     387     354      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     853     885    1644 
   Size:        853      32     759

  Fragments arranged by size: 

                 853     759      32
 
MscI TGG'CCA

Cuts at:      0    1192    1516    1644 
   Size:       1192     324     128

  Fragments arranged by size: 

                1192     324     128
 
MslI CAynn'nnrTG

Cuts at:      0     536    1501    1570    1576    1644 
   Size:        536     965      69       6      68

  Fragments arranged by size: 

                 965     536      69      68       6
 
MspA1I CmG'CkG

Cuts at:      0      39     445    1148    1391    1644 
   Size:         39     406     703     243     253

  Fragments arranged by size: 

                 703     406     253     243      39
 
NcoI C'CATG_G

Cuts at:      0    1577    1644 
   Size:       1577      67
 
NspI r_CATG'y

Cuts at:      0    1500    1569    1644 
   Size:       1500      69      75

  Fragments arranged by size: 

                1500      75      69
 
PasI CC'CwG_GG

Cuts at:      0      28     939    1644 
   Size:         28     911     705

  Fragments arranged by size: 

                 911     705      28
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1599    1644 
   Size:       1599      45
 
PfoI T'CCnGG_A

Cuts at:      0     544     809    1644 
   Size:        544     265     835

  Fragments arranged by size: 

                 835     544     265
 
PpuMI rG'GwC_Cy

Cuts at:      0      33     240     306     390    1644 
   Size:         33     207      66      84    1254

  Fragments arranged by size: 

                1254     207      84      66      33
 
PspOMI G'GGCC_C

Cuts at:      0     680     942    1528    1644 
   Size:        680     262     586     116

  Fragments arranged by size: 

                 680     586     262     116
 
PstI C_TGCA'G

Cuts at:      0     606    1022    1616    1644 
   Size:        606     416     594      28

  Fragments arranged by size: 

                 606     594     416      28
 
PvuII CAG'CTG

Cuts at:      0     445    1644 
   Size:        445    1199
 
SacI G_AGCT'C

Cuts at:      0    1607    1644 
   Size:       1607      37
 
SacII CC_GC'GG

Cuts at:      0      40    1149    1644 
   Size:         40    1109     495

  Fragments arranged by size: 

                1109     495      40
 
SanDI GG'GwC_CC

Cuts at:      0      33    1644 
   Size:         33    1611
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1300    1456    1644 
   Size:       1300     156     188

  Fragments arranged by size: 

                1300     188     156
 
SexAI A'CCwGG_T

Cuts at:      0    1052    1592    1644 
   Size:       1052     540      52

  Fragments arranged by size: 

                1052     540      52
 
SfcI C'TryA_G

Cuts at:      0     602    1018    1612    1644 
   Size:        602     416     594      32

  Fragments arranged by size: 

                 602     594     416      32
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     950    1644 
   Size:        950     694
 
SmlI C'TyrA_G

Cuts at:      0     966    1644 
   Size:        966     678
 
SphI G_CATG'C

Cuts at:      0    1569    1644 
   Size:       1569      75
 
StyI C'CwwG_G

Cuts at:      0     464    1577    1644 
   Size:        464    1113      67

  Fragments arranged by size: 

                1113     464      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     216     933    1644 
   Size:        216     717     711

  Fragments arranged by size: 

                 717     711     216
 
TatI w'GTAC_w

Cuts at:      0     644    1644 
   Size:        644    1000
 
XmnI GAAnn'nnTTC

Cuts at:      0    1294    1644 
   Size:       1294     350
 
ZraI GAC'GTC

Cuts at:      0    1266    1644 
   Size:       1266     378
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AhdI     AleI     AlfI    AlwNI     ApaI
     AvaI     BanI    BanII    BbvCI     BglI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
   BssHII   BstAPI    BstXI   Bsu36I     BtgI    BtgZI     BtsI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
    SexAI     SfcI     SfiI     SmlI     SphI     StyI    TaqII     TatI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaXI    BsiWI    BspHI
    BspMI    BsrFI    BsrGI    BssSI    BstBI   BstEII    BstYI  BstZ17I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SbfI     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI
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