Restriction map for pF1KE1798
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       BbvCI       BcgI        
BfrI        BglII       BlpI        Bpu1102I    BsaBI       
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       FseI        FspI        HpaI        
KpnI        MamI        MfeI        MluI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Alw44I       1621  GTGCAC               G^TGCAC
AlwNI        1729  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1621  GTGCAC               G^TGCAC
AvrII         162  CCTAGG               C^CTAGG
BamHI        1065  GGATCC               G^GATCC
BbeI           28  GGCGCC               GGCGC^C
BbrPI         917  CACGTG               CAC^GTG
BbsI         1737  GAAGAC               GAAGAC(2/6)
BciVI        1966  GTATCC               GTATCC(6/5)
BclI         1467  TGATCA               T^GATCA
BcnI         2370  CCCGG                CC^[GC]GG
BglI         2212  GCCCCTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          162  CCTAGG               C^CTAGG
BpuAI        1737  GAAGAC               GAAGAC(2/6)
BsaAI         917  CACGTG               [CT]AC^GT[GA]
BsaI         2374  GAGACC               GGTCTC(1/5)
BsmFI          37  GGGAC                GGGAC(10/14)
Bsp1407I     1835  TGTACA               T^GTACA
BsrDI        1234  GCAATG               GCAATG(2/0)
BsrGI        1835  TGTACA               T^GTACA
BssSI          88  CACGAG               CACGAG(-5/-1)
DsaI           32  CCGCGG               C^C[GA][CT]GG
EcoRV         798  GATATC               GAT^ATC
EcoT22I      1603  ATGCAT               ATGCA^T
FbaI         1467  TGATCA               T^GATCA
HaeII          28  GGCGCC               [GA]GCGC^[CT]
HincII        706  GTTGAC               GT[CT]^[GA]AC
HindII        706  GTTGAC               GT[CT]^[GA]AC
KasI           28  GGCGCC               G^GCGCC
KspI           32  CCGCGG               CCGC^GG
NarI           28  GGCGCC               GG^CGCC
NciI         2370  CCCGG                CC^[GC]GG
NsiI         1603  ATGCAT               ATGCA^T
PflMI         504  CCACAAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         917  CACGTG               CAC^GTG
PmlI          917  CACGTG               CAC^GTG
PpuMI         158  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI         2338  CTGCAG               CTGCA^G
SacII          32  CCGCGG               CCGC^GG
SfcI         2338  CTGCAG               C^T[GA][CT]AG
SfoI           28  GGCGCC               GGC^GCC
SspBI        1835  TGTACA               T^GTACA
Van91I        504  CCACAAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          893  CCAAGAAGAGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII       2100  TCCGGA               T^CCGGA
             2119  TCCGGA              
AcyI           28  GGCGCC               G[GA]^CG[CT]C
             1825  GGCGTC              
AflIII       1478  ACACGT               A^C[GA][CT]GT
             1888  ACGTGT              
Asp700I      1456  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1539  GAAGCACTTC          
BalI          210  TGGCCA               TGG^CCA
              564  TGGCCA              
BanI           28  GGCGCC               G^G[CT][GA]CC
              660  GGCACC              
BanII         148  GAGCTC               G[GA]GC[CT]^C
             1774  GAGCTC              
BsaHI          28  GGCGCC               G[GA]^CG[CT]C
             1825  GGCGTC              
BseAI        2100  TCCGGA               T^CCGGA
             2119  TCCGGA              
BsgI          995  CTGCAC               GTGCAG(16/14)
              460  GTGCAG              
BsiYI         504  CCACAAGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2385  CCAGTTCTCGG         
BslI          504  CCACAAGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2385  CCAGTTCTCGG         
BsmBI        1552  GAGACG               CGTCTC(1/5)
             2260  GAGACG              
BspEI        2100  TCCGGA               T^CCGGA
             2119  TCCGGA              
BstAPI         87  GCACGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              453  GCATGAGGTGC         
BstXI         213  CCATGATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1895  CCAGCGCGGTGG        
DraII         158  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2210  AGGCCCC             
EaeI          210  TGGCCA               [CT]^GGCC[GA]
              564  TGGCCA              
EcoO109I      158  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2210  AGGCCCC             
EcoT14I       162  CCTAGG               C^C[AT][AT]GG
             1992  CCAAGG              
FauI         1081  CCCGC                CCCGC(4/6)
               34  GCGGG               
Hin1I          28  GGCGCC               G[GA]^CG[CT]C
             1825  GGCGTC              
MluNI         210  TGGCCA               TGG^CCA
              564  TGGCCA              
MroI         2100  TCCGGA               T^CCGGA
             2119  TCCGGA              
MscI          210  TGGCCA               TGG^CCA
              564  TGGCCA              
NspI          117  ACATGC               [GA]CATG^[CT]
             1601  ACATGC              
SacI          148  GAGCTC               GAGCT^C
             1774  GAGCTC              
SfaNI         922  GATGC                GCATC(5/9)
              754  GCATC               
SmlI          538  CTTGAG               C^T[CT][GA]AG
             2040  CTTGAG              
StyI          162  CCTAGG               C^C[AT][AT]GG
             1992  CCAAGG              
Tsp45I       1213  GTGAC                ^GT[GC]AC
             1808  GTGAC               
XmnI         1456  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1539  GAAGCACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsmAI       Bsp1286I    BspHI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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