Restriction map for pF1KE1798
 (Linear) (Six-base) MAPSORT of: KIEE1798.seq  Check: 8438  from: 1  to: 2406



KIEE1798.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1165    1530    1756    2179    2406 
   Size:        175     990     365     226     423     227

  Fragments arranged by size: 

                 990     423     365     227     226     175
 
AflIII A'CryG_T

Cuts at:      0    1478    1888    2406 
   Size:       1478     410     518

  Fragments arranged by size: 

                1478     518     410
 
AleI CACnn'nnGTG

Cuts at:      0    1547    2406 
   Size:       1547     859
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     293     325    2406 
   Size:        293      32    2081

  Fragments arranged by size: 

                2081     293      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1734    2406 
   Size:       1734     672
 
ApaLI G'TGCA_C

Cuts at:      0    1621    2406 
   Size:       1621     785
 
ApoI r'AATT_y

Cuts at:      0     141     783    1644    2406 
   Size:        141     642     861     762

  Fragments arranged by size: 

                 861     762     642     141
 
AvrII C'CTAG_G

Cuts at:      0     162    2406 
   Size:        162    2244
 
BamHI G'GATC_C

Cuts at:      0    1065    2406 
   Size:       1065    1341
 
BanI G'GyrC_C

Cuts at:      0      28     660    2406 
   Size:         28     632    1746

  Fragments arranged by size: 

                1746     632      28
 
BanII G_rGCy'C

Cuts at:      0     152    1778    2406 
   Size:        152    1626     628

  Fragments arranged by size: 

                1626     628     152
 
BbeI G_GCGC'C

Cuts at:      0      32    2406 
   Size:         32    2374
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1744    2406 
   Size:       1744     662
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1977    2406 
   Size:       1977     429
 
BclI T'GATC_A

Cuts at:      0    1467    2406 
   Size:       1467     939
 
BfrBI ATG'CAT

Cuts at:      0    1605    2406 
   Size:       1605     801
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2218    2406 
   Size:       2218     188
 
Bme1580I G_kGCm'C

Cuts at:      0    1625    2406 
   Size:       1625     781
 
BmgBI CAC'GTC

Cuts at:      0    1889    2406 
   Size:       1889     517
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      88    2406 
   Size:         88    2318
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     770    1229    2406 
   Size:        191     579     459    1177

  Fragments arranged by size: 

                1177     579     459     191
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559    2061    2406 
   Size:        559    1502     345

  Fragments arranged by size: 

                1502     559     345
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2368    2406 
   Size:       2368      38
 
BsaAI yAC'GTr

Cuts at:      0     919    2406 
   Size:        919    1487
 
BsaHI Gr'CG_yC

Cuts at:      0      29    1826    2406 
   Size:         29    1797     580

  Fragments arranged by size: 

                1797     580      29
 
BsaWI w'CCGG_w

Cuts at:      0    1719    2100    2119    2406 
   Size:       1719     381      19     287

  Fragments arranged by size: 

                1719     381     287      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     467     497     708     738    1062    1092    2114    2144 
   Size:        467      30     211      30     324      30    1022      30

Cuts at:   2144    2406 
   Size:        262

  Fragments arranged by size: 

                1022     467     324     262     211      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19      22      64      77    1523    2406 
   Size:         19       3      42      13    1446     883

  Fragments arranged by size: 

                1446     883      42      19      13       3
 
BseYI C'CCAG_C

Cuts at:      0    2344    2406 
   Size:       2344      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     980    2406 
   Size:        481     499    1426

  Fragments arranged by size: 

                1426     499     481
 
BsiHKAI G_wGCw'C

Cuts at:      0     152    1625    1778    2406 
   Size:        152    1473     153     628

  Fragments arranged by size: 

                1473     628     153     152
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1546    2254    2406 
   Size:       1546     708     152

  Fragments arranged by size: 

                1546     708     152
 
Bsp1286I G_dGCh'C

Cuts at:      0     152    1625    1778    2406 
   Size:        152    1473     153     628

  Fragments arranged by size: 

                1473     628     153     152
 
BspEI T'CCGG_A

Cuts at:      0    2100    2119    2406 
   Size:       2100      19     287

  Fragments arranged by size: 

                2100     287      19
 
BspHI T'CATG_A

Cuts at:      0    1464    1571    1872    1910    2406 
   Size:       1464     107     301      38     496

  Fragments arranged by size: 

                1464     496     301     107      38
 
BsrDI GCAATG_nn'

Cuts at:      0    1241    2406 
   Size:       1241    1165
 
BsrGI T'GTAC_A

Cuts at:      0    1835    2406 
   Size:       1835     571
 
BssSI C'ACGA_G

Cuts at:      0      88    2406 
   Size:         88    2318
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      93     459    2406 
   Size:         93     366    1947

  Fragments arranged by size: 

                1947     366      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     220    1902    2406 
   Size:        220    1682     504

  Fragments arranged by size: 

                1682     504     220
 
BstYI r'GATC_y

Cuts at:      0     888    1065    1629    2010    2113    2406 
   Size:        888     177     564     381     103     293

  Fragments arranged by size: 

                 888     564     381     293     177     103
 
BtgI C'CryG_G

Cuts at:      0      32    2406 
   Size:         32    2374
 
BtsI GCAGTG_nn'

Cuts at:      0    2335    2406 
   Size:       2335      71
 
EaeI y'GGCC_r

Cuts at:      0     210     564    2406 
   Size:        210     354    1842

  Fragments arranged by size: 

                1842     354     210
 
EarI CTCTTCn'nnn_

Cuts at:      0     892     952    1043    1184    2386    2406 
   Size:        892      60      91     141    1202      20

  Fragments arranged by size: 

                1202     892     141      91      60      20
 
EcoICRI GAG'CTC

Cuts at:      0     150    1776    2406 
   Size:        150    1626     630

  Fragments arranged by size: 

                1626     630     150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     191     770    1165    1229    1530    1756    2179 
   Size:        175      16     579     395      64     301     226     423

Cuts at:   2179    2406 
   Size:        227

  Fragments arranged by size: 

                 579     423     395     301     227     226     175      64
                  16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159    2211    2406 
   Size:        159    2052     195

  Fragments arranged by size: 

                2052     195     159
 
EcoRV GAT'ATC

Cuts at:      0     800    2406 
   Size:        800    1606
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1560    2406 
   Size:       1560     846
 
HaeII r_GCGC'y

Cuts at:      0      32    2406 
   Size:         32    2374
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     873     905    2406 
   Size:        507      32     334      32    1501

  Fragments arranged by size: 

                1501     507     334      32      32
 
HincII GTy'rAC

Cuts at:      0     708    2406 
   Size:        708    1698
 
HindIII A'AGCT_T

Cuts at:      0     535    1349    1579    2406 
   Size:        535     814     230     827

  Fragments arranged by size: 

                 827     814     535     230
 
KasI G'GCGC_C

Cuts at:      0      28    2406 
   Size:         28    2378
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     453    1593    2406 
   Size:        453    1140     813

  Fragments arranged by size: 

                1140     813     453
 
MscI TGG'CCA

Cuts at:      0     212     566    2406 
   Size:        212     354    1840

  Fragments arranged by size: 

                1840     354     212
 
MslI CAynn'nnrTG

Cuts at:      0    1060    1547    1604    2138    2406 
   Size:       1060     487      57     534     268

  Fragments arranged by size: 

                1060     534     487     268      57
 
MspA1I CmG'CkG

Cuts at:      0      23      34    1165    1591    2076    2406 
   Size:         23      11    1131     426     485     330

  Fragments arranged by size: 

                1131     485     426     330      23      11
 
NarI GG'CG_CC

Cuts at:      0      29    2406 
   Size:         29    2377
 
NsiI A_TGCA'T

Cuts at:      0    1607    2406 
   Size:       1607     799
 
NspI r_CATG'y

Cuts at:      0     121    1605    2406 
   Size:        121    1484     801

  Fragments arranged by size: 

                1484     801     121
 
PasI CC'CwG_GG

Cuts at:      0    1293    2406 
   Size:       1293    1113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     510    2406 
   Size:        510    1896
 
PfoI T'CCnGG_A

Cuts at:      0     311    1206    2406 
   Size:        311     895    1200

  Fragments arranged by size: 

                1200     895     311
 
PmlI CAC'GTG

Cuts at:      0     919    2406 
   Size:        919    1487
 
PpuMI rG'GwC_Cy

Cuts at:      0     159    2406 
   Size:        159    2247
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     179     211    2406 
   Size:        179      32    2195

  Fragments arranged by size: 

                2195     179      32
 
PstI C_TGCA'G

Cuts at:      0    2342    2406 
   Size:       2342      64
 
PvuII CAG'CTG

Cuts at:      0    1165    1591    2076    2406 
   Size:       1165     426     485     330

  Fragments arranged by size: 

                1165     485     426     330
 
SacI G_AGCT'C

Cuts at:      0     152    1778    2406 
   Size:        152    1626     628

  Fragments arranged by size: 

                1626     628     152
 
SacII CC_GC'GG

Cuts at:      0      35    2406 
   Size:         35    2371
 
SfcI C'TryA_G

Cuts at:      0    2338    2406 
   Size:       2338      68
 
SfoI GGC'GCC

Cuts at:      0      30    2406 
   Size:         30    2376
 
SmlI C'TyrA_G

Cuts at:      0     538    2040    2406 
   Size:        538    1502     366

  Fragments arranged by size: 

                1502     538     366
 
SspI AAT'ATT

Cuts at:      0     546     812    1440    2406 
   Size:        546     266     628     966

  Fragments arranged by size: 

                 966     628     546     266
 
StyI C'CwwG_G

Cuts at:      0     162    1992    2406 
   Size:        162    1830     414

  Fragments arranged by size: 

                1830     414     162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     712    1892    2340    2406 
   Size:        712    1180     448      66

  Fragments arranged by size: 

                1180     712     448      66
 
TatI w'GTAC_w

Cuts at:      0    1835    2406 
   Size:       1835     571
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     467     499     708     740    1062    1094    1995    2027 
   Size:        467      32     209      32     322      32     901      32

Cuts at:   2027    2114    2146    2406 
   Size:         87      32     260

  Fragments arranged by size: 

                 901     467     322     260     209      87      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     900    2406 
   Size:        900    1506
 
XmnI GAAnn'nnTTC

Cuts at:      0    1460    1543    2406 
   Size:       1460      83     863

  Fragments arranged by size: 

                1460     863      83
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AloI    AlwNI    ApaLI     ApoI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI
     BglI Bme1580I    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrDI    BsrGI    BssSI   BstAPI    BstXI
    BstYI     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI
     MscI     MslI   MspA1I     NarI     NsiI     NspI     PasI    PflMI
     PfoI     PmlI    PpuMI     PsrI     PstI    PvuII     SacI    SacII
     SfcI     SfoI     SmlI     SspI     StyI    TaqII     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     ApaI     AscI     AseI    AsiSI     AvaI     BaeI
    BbvCI     BcgI    BglII     BlpI     BmrI     BmtI   Bpu10I    BsaBI
    BsiEI    BsiWI     BsmI    BspMI    BsrBI    BsrFI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRI     FseI     FspI    FspAI
     HpaI     KpnI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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