Restriction map for pF1KE1971
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# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BcgI        
BfrI        BglI        BlnI        BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PinAI       
PmeI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   66 enzymes) #
###################################

AccI         1280  GTATAC               GT^[AC][GT]AC
AfaI         1437  GTAC                 GT^AC
AlwNI        1456  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          382  ATTAAT               AT^TAAT
AsnI          382  ATTAAT               AT^TAAT
Asp700I      1000  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          85  GAGCAC               G[AT]GC[AT]^C
AvaII        1083  GGACC                G^G[AT]CC
BalI         1385  TGGCCA               TGG^CCA
BanI          129  GGTGCC               G^G[CT][GA]CC
BanII         461  GGGCTC               G[GA]GC[CT]^C
BbrPI          88  CACGTG               CAC^GTG
BbsI         1600  GTCTTC               GAAGAC(2/6)
BbvCI        1567  CCTCAGC              CCTCAGC(-5/-2)
BclI          887  TGATCA               T^GATCA
BcnI         1356  CCCGG                CC^[GC]GG
BfaI          419  CTAG                 C^TAG
BglII         228  AGATCT               A^GATCT
BlpI           82  GCTGAGC              GC^T[ACGT]AGC
BpmI          319  CTCCAG               CTGGAG(16/14)
Bpu1102I       82  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1600  GTCTTC               GAAGAC(2/6)
BsaAI          88  CACGTG               [CT]AC^GT[GA]
BsgI         1035  CTGCAC               GTGCAG(16/14)
BsiHKAI        85  GAGCAC               G[AT]GC[AT]^C
BsrBI         146  GAGCGG               CCGCTC(-3/-3)
Bst1107I     1280  GTATAC               GTA^TAC
BstYI         228  AGATCT               [GA]^GATC[CT]
BstZ17I      1280  GTATAC               GTA^TAC
Cac8I         686  GCCTGC               GC[ACGT]^[ACGT]GC
CelII          82  GCTGAGC              GC^T[ACGT]AGC
DraI          169  TTTAAA               TTT^AAA
DraII        1166  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI         1125  CCATGG               C^C[GA][CT]GG
EaeI         1385  TGGCCA               [CT]^GGCC[GA]
EcoO109I     1166  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         715  GAATTC               G^AATTC
EcoT14I      1125  CCATGG               C^C[AT][AT]GG
FbaI          887  TGATCA               T^GATCA
HapII        1357  CCGG                 C^CGG
HpaII        1357  CCGG                 C^CGG
MaeI          419  CTAG                 C^TAG
MaeII          89  ACGT                 A^CGT
MflI          228  AGATCT               [GA]^GATC[CT]
MluNI        1385  TGGCCA               TGG^CCA
MscI         1385  TGGCCA               TGG^CCA
MspI         1357  CCGG                 C^CGG
NciI         1356  CCCGG                CC^[GC]GG
NcoI         1125  CCATGG               C^CATGG
PaeR7I       1478  CTCGAG               C^TCGAG
PflMI        1074  CCAACTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          88  CACGTG               CAC^GTG
PmlI           88  CACGTG               CAC^GTG
PshBI         382  ATTAAT               AT^TAAT
PstI         1421  CTGCAG               CTGCA^G
RsaI         1437  GTAC                 GT^AC
SexAI        1432  ACCTGGT              A^CC[AT]GGT
StyI         1125  CCATGG               C^C[AT][AT]GG
TaqI         1479  TCGA                 T^CGA
Tsp45I       1446  GTCAC                ^GT[GC]AC
TthHB8I      1479  TCGA                 T^CGA
Van91I       1074  CCAACTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          563  CCAAATGCAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1478  CTCGAG               C^TCGAG
XhoII         228  AGATCT               [GA]^GATC[CT]
XmnI         1000  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI          268  CCGC                 CCGC(-3/-1)
              148  GCGG                
AvaI          872  CTCGGG               C^[CT]CG[GA]G
             1478  CTCGAG              
BciVI         877  GGATAC               GTATCC(6/5)
             1115  GTATCC              
BsmFI         110  GGGAC                GGGAC(10/14)
              950  GGGAC               
BsoBI         872  CTCGGG               C^[CT]CG[GA]G
             1478  CTCGAG              
BspMI         590  ACCTGC               ACCTGC(4/8)
              101  GCAGGT              
Cfr13I       1083  GGACC                G^G[ACGT]CC
             1167  GGCCC               
HphI         1336  TCACC                GGTGA(8/7)
             1496  TCACC               
MaeIII       1091  GTAAC                ^GT[ACGT]AC
             1446  GTCAC               
MslI          606  CATAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              967  CACTTGGATG          
MwoI          270  GCAAAGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1103  GCTTCATCTGC         
Sau96I       1083  GGACC                G^G[ACGT]CC
             1167  GGCCC               
SfcI          477  CTACAG               C^T[GA][CT]AG
             1421  CTGCAG              
SmlI          241  CTCAAG               C^T[CT][GA]AG
             1478  CTCGAG              
TfiI          829  GAATC                G^A[AT]TC
             1297  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       ItaI        Ksp632I     
MboI        MboII       MnlI        MseI        MvaI        
NdeII       NlaIII      NlaIV       PleI        SapI        
Sau3AI      ScrFI       SfaNI       Tru9I       TseI        
Tsp509I     TspRI       
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