Restriction map for pF1KE1971
 (Linear) (Six-base) MAPSORT of: KIEE1971.seq  Check: 3303  from: 1  to: 1614



KIEE1971.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0    1281    1614 
   Size:       1281     333
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     382     430     712    1529    1614 
   Size:        168     214      48     282     817      85

  Fragments arranged by size: 

                 817     282     214     168      85      48
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1461    1614 
   Size:       1461     153
 
ApoI r'AATT_y

Cuts at:      0     467     715     760    1614 
   Size:        467     248      45     854

  Fragments arranged by size: 

                 854     467     248      45
 
AseI AT'TA_AT

Cuts at:      0     383    1614 
   Size:        383    1231
 
AvaI C'yCGr_G

Cuts at:      0     872    1478    1614 
   Size:        872     606     136

  Fragments arranged by size: 

                 872     606     136
 
BanI G'GyrC_C

Cuts at:      0     129    1614 
   Size:        129    1485
 
BanII G_rGCy'C

Cuts at:      0     465    1614 
   Size:        465    1149
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1593    1614 
   Size:       1593      21
 
BbvCI CC'TCA_GC

Cuts at:      0    1568    1614 
   Size:       1568      46
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     871    1126    1614 
   Size:        871     255     488

  Fragments arranged by size: 

                 871     488     255
 
BclI T'GATC_A

Cuts at:      0     887    1614 
   Size:        887     727
 
BglII A'GATC_T

Cuts at:      0     228    1614 
   Size:        228    1386
 
BlpI GC'TnA_GC

Cuts at:      0      83    1614 
   Size:         83    1531
 
Bme1580I G_kGCm'C

Cuts at:      0     672     932    1213    1614 
   Size:        672     260     281     401

  Fragments arranged by size: 

                 672     401     281     260
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     576    1614 
   Size:        576    1038
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1614 
   Size:        304    1310
 
Bpu10I CC'TnA_GC

Cuts at:      0     250    1568    1614 
   Size:        250    1318      46

  Fragments arranged by size: 

                1318     250      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226    1614 
   Size:        226    1388
 
BsaAI yAC'GTr

Cuts at:      0      90    1614 
   Size:         90    1524
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796    1614 
   Size:        766      30     818

  Fragments arranged by size: 

                 818     766      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     442     790    1318    1614 
   Size:        442     348     528     296

  Fragments arranged by size: 

                 528     442     348     296
 
BseYI C'CCAG_C

Cuts at:      0    1377    1614 
   Size:       1377     237
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1020    1614 
   Size:       1020     594
 
BsiHKAI G_wGCw'C

Cuts at:      0      89    1614 
   Size:         89    1525
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     465     672     932    1213    1614 
   Size:         89     376     207     260     281     401

  Fragments arranged by size: 

                 401     376     281     260     207      89
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      92     599    1614 
   Size:         92     507    1015

  Fragments arranged by size: 

                1015     507      92
 
BsrBI CCG'CTC

Cuts at:      0     148    1614 
   Size:        148    1466
 
BstYI r'GATC_y

Cuts at:      0     228    1614 
   Size:        228    1386
 
BstZ17I GTA'TAC

Cuts at:      0    1282    1614 
   Size:       1282     332
 
BtgI C'CryG_G

Cuts at:      0    1125    1614 
   Size:       1125     489
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     139    1485    1499    1614 
   Size:        139    1346      14     115

  Fragments arranged by size: 

                1346     139     115      14
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     978    1013    1614 
   Size:        978      35     601

  Fragments arranged by size: 

                 978     601      35
 
DraI TTT'AAA

Cuts at:      0     171    1614 
   Size:        171    1443
 
EaeI y'GGCC_r

Cuts at:      0    1385    1614 
   Size:       1385     229
 
EarI CTCTTCn'nnn_

Cuts at:      0       9     379     613     995    1614 
   Size:          9     370     234     382     619

  Fragments arranged by size: 

                 619     382     370     234       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     304     382     430     712    1529    1614 
   Size:        168     136      78      48     282     817      85

  Fragments arranged by size: 

                 817     282     168     136      85      78      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1167    1614 
   Size:       1167     447
 
EcoRI G'AATT_C

Cuts at:      0     715    1614 
   Size:        715     899
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     198     315    1614 
   Size:        198     117    1299

  Fragments arranged by size: 

                1299     198     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252     814     846    1171    1203    1318    1350 
   Size:        220      32     562      32     325      32     115      32

Cuts at:   1350    1614 
   Size:        264

  Fragments arranged by size: 

                 562     325     264     220     115      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     626    1614 
   Size:        317     309     988

  Fragments arranged by size: 

                 988     317     309
 
MscI TGG'CCA

Cuts at:      0    1387    1614 
   Size:       1387     227
 
MslI CAynn'nnrTG

Cuts at:      0     610     971    1614 
   Size:        610     361     643

  Fragments arranged by size: 

                 643     610     361
 
NcoI C'CATG_G

Cuts at:      0    1125    1614 
   Size:       1125     489
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1080    1614 
   Size:       1080     534
 
PfoI T'CCnGG_A

Cuts at:      0     320    1355    1614 
   Size:        320    1035     259

  Fragments arranged by size: 

                1035     320     259
 
PmlI CAC'GTG

Cuts at:      0      90    1614 
   Size:         90    1524
 
PspXI vC'TCGA_Gb

Cuts at:      0    1478    1614 
   Size:       1478     136
 
PstI C_TGCA'G

Cuts at:      0    1425    1614 
   Size:       1425     189
 
SapI GCTCTTCn'nnn_

Cuts at:      0       9     379     995    1614 
   Size:          9     370     616     619

  Fragments arranged by size: 

                 619     616     370       9
 
SexAI A'CCwGG_T

Cuts at:      0    1432    1614 
   Size:       1432     182
 
SfcI C'TryA_G

Cuts at:      0     477    1421    1614 
   Size:        477     944     193

  Fragments arranged by size: 

                 944     477     193
 
SmlI C'TyrA_G

Cuts at:      0     241    1478    1614 
   Size:        241    1237     136

  Fragments arranged by size: 

                1237     241     136
 
StyI C'CwwG_G

Cuts at:      0    1125    1614 
   Size:       1125     489
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     766     798    1614 
   Size:        766      32     816

  Fragments arranged by size: 

                 816     766      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     570    1614 
   Size:        570    1044
 
XhoI C'TCGA_G

Cuts at:      0    1478    1614 
   Size:       1478     136
 
XmnI GAAnn'nnTTC

Cuts at:      0    1004    1614 
   Size:       1004     610
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI     AvaI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BstYI  BstZ17I     BtgI    BtgZI
    CspCI     DraI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI
    Hin4I     MmeI     MscI     MslI     NcoI    PflMI     PfoI     PmlI
    PspXI     PstI     SapI    SexAI     SfcI     SmlI     StyI     TstI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BcgI    BfrBI     BglI    BmgBI
     BmrI     BmtI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtsI     ClaI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XmaI
     ZraI
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