Restriction map for pF1KE2408
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        AhdI        Aor51HI     ApaI        AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BcnI        BfrI        BglI        
BlnI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
FauI        FseI        FspI        HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        2588  GACGTC               GACGT^C
AflIII       3194  ACATGT               A^C[GA][CT]GT
AocI         1039  CCTCAGG              CC^T[ACGT]AGG
AseI         1555  ATTAAT               AT^TAAT
AsnI         1555  ATTAAT               AT^TAAT
BanI         2291  GGCGCC               G^G[CT][GA]CC
BbeI         2291  GGCGCC               GGCGC^C
BcgI         5565  CGAAAGCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3650  TGATCA               T^GATCA
BspLU11I     3194  ACATGT               A^CATGT
BsrFI        4151  GCCGGT               [GA]^CCGG[CT]
Bsu36I       1039  CCTCAGG              CC^T[ACGT]AGG
CfoI         2292  GCGC                 GCG^C
Cfr10I       4151  GCCGGT               [GA]^CCGG[CT]
DraI         4305  TTTAAA               TTT^AAA
DraIII       4697  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          587  CCATGG               C^C[GA][CT]GG
Eco81I       1039  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      6242  ATGCAT               ATGCA^T
FbaI         3650  TGATCA               T^GATCA
HaeII        2291  GGCGCC               [GA]GCGC^[CT]
HapII        4152  CCGG                 C^CGG
HhaI         2292  GCGC                 GCG^C
HinP1I       2292  GCGC                 G^CGC
HpaII        4152  CCGG                 C^CGG
KasI         2291  GGCGCC               G^GCGCC
MspI         4152  CCGG                 C^CGG
NarI         2291  GGCGCC               GG^CGCC
NcoI          587  CCATGG               C^CATGG
NsiI         6242  ATGCAT               ATGCA^T
PaeR7I       5336  CTCGAG               C^TCGAG
PshBI        1555  ATTAAT               AT^TAAT
PstI         5078  CTGCAG               CTGCA^G
SacI         2879  GAGCTC               GAGCT^C
SalI         2600  GTCGAC               G^TCGAC
SexAI         896  ACCAGGT              A^CC[AT]GGT
SfoI         2291  GGCGCC               GGC^GCC
SphI         3664  GCATGC               GCATG^C
XhoI         5336  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Alw44I       4485  GTGCAC               G^TGCAC
             5573  GTGCAC              
ApaLI        4485  GTGCAC               G^TGCAC
             5573  GTGCAC              
AvaI         2738  CTCGGG               C^[CT]CG[GA]G
             5336  CTCGAG              
BalI         4131  TGGCCA               TGG^CCA
             6447  TGGCCA              
BanII        2879  GAGCTC               G[GA]GC[CT]^C
             5683  GGGCTC              
BbvCI          26  CCTCAGC              CCTCAGC(-5/-2)
             2782  GCTGAGG             
BciVI        3463  GGATAC               GTATCC(6/5)
             6354  GTATCC              
BglII        4344  AGATCT               A^GATCT
             5412  AGATCT              
BlpI         2881  GCTCAGC              GC^T[ACGT]AGC
             3200  GCTTAGC             
Bpu1102I     2881  GCTCAGC              GC^T[ACGT]AGC
             3200  GCTTAGC             
BsmBI        4103  CGTCTC               CGTCTC(1/5)
             2586  GAGACG              
BsoBI        2738  CTCGGG               C^[CT]CG[GA]G
             5336  CTCGAG              
BspHI         438  TCATGA               T^CATGA
             6234  TCATGA              
BspMI        2142  ACCTGC               ACCTGC(4/8)
             5076  ACCTGC              
BsrBI        3082  GAGCGG               CCGCTC(-3/-3)
             5641  GAGCGG              
BstAPI       2311  GCATATTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2463  GCAGGGAATGC         
BstEII         17  GGTTACC              G^GT[ACGT]ACC
             4012  GGTGACC             
BstPI          17  GGTTACC              G^GT[ACGT]ACC
             4012  GGTGACC             
CelII        2881  GCTCAGC              GC^T[ACGT]AGC
             3200  GCTTAGC             
DraII        5174  AGGACCT              [GA]G^G[ACGT]CC[CT]
             6191  AGGACCT             
DrdI         4752  GACTCCTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5994  GACTAATGAGTC        
EaeI         4131  TGGCCA               [CT]^GGCC[GA]
             6447  TGGCCA              
EcoO109I     5174  AGGACCT              [GA]G^G[ACGT]CC[CT]
             6191  AGGACCT             
EcoO65I        17  GGTTACC              G^GT[ACGT]ACC
             4012  GGTGACC             
EcoRI         404  GAATTC               G^AATTC
             2626  GAATTC              
EcoRV        2389  GATATC               GAT^ATC
             3988  GATATC              
MluNI        4131  TGGCCA               TGG^CCA
             6447  TGGCCA              
MscI         4131  TGGCCA               TGG^CCA
             6447  TGGCCA              
PpuMI        5174  AGGACCT              [GA]G^G[AT]CC[CT]
             6191  AGGACCT             
RcaI          438  TCATGA               T^CATGA
             6234  TCATGA              
SfcI         4617  CTACAG               C^T[GA][CT]AG
             5078  CTGCAG              
SspI         3334  AATATT               AAT^ATT
             4930  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoNI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XbaI        XcmI        XhoII       
XmnI        
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