Restriction map for pF1KE2408
 (Linear) (Six-base) MAPSORT of: KIEE2408.seq  Check: 128  from: 1  to: 6504



KIEE2408.orf

 With 182 enzymes: SgfI * 

                        June 11, 2012 13:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2151    6504 
   Size:       2151    4353
 
AatII G_ACGT'C

Cuts at:      0    2592    6504 
   Size:       2592    3912
 
AccI GT'mk_AC

Cuts at:      0     234    1129    2601    6504 
   Size:        234     895    1472    3903

  Fragments arranged by size: 

                3903    1472     895     234
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1271    1372    1832    2009    2372    2397    3610 
   Size:         62    1209     101     460     177     363      25    1213

Cuts at:   3610    3617    3788    4363    4843    4876    5051    6062    6143 
   Size:          7     171     575     480      33     175    1011      81

Cuts at:   6143    6504 
   Size:        361

  Fragments arranged by size: 

                1213    1209    1011     575     480     460     363     361
                 177     175     171     101      81      62      33      25
                   7
 
AflIII A'CryG_T

Cuts at:      0    3194    6504 
   Size:       3194    3310
 
AleI CACnn'nnGTG

Cuts at:      0    1115    4915    6504 
   Size:       1115    3800    1589

  Fragments arranged by size: 

                3800    1589    1115
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4500    6504 
   Size:       4500    2004
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2107    3436    5473    6504 
   Size:       2107    1329    2037    1031

  Fragments arranged by size: 

                2107    2037    1329    1031
 
ApaLI G'TGCA_C

Cuts at:      0    4485    5573    6504 
   Size:       4485    1088     931

  Fragments arranged by size: 

                4485    1088     931
 
ApoI r'AATT_y

Cuts at:      0     262     404    2626    2888    5240    5548    6504 
   Size:        262     142    2222     262    2352     308     956

  Fragments arranged by size: 

                2352    2222     956     308     262     262     142
 
AseI AT'TA_AT

Cuts at:      0    1556    6504 
   Size:       1556    4948
 
AvaI C'yCGr_G

Cuts at:      0    2738    5336    6504 
   Size:       2738    2598    1168

  Fragments arranged by size: 

                2738    2598    1168
 
BanI G'GyrC_C

Cuts at:      0    2291    6504 
   Size:       2291    4213
 
BanII G_rGCy'C

Cuts at:      0    2883    5687    6504 
   Size:       2883    2804     817

  Fragments arranged by size: 

                2883    2804     817
 
BbeI G_GCGC'C

Cuts at:      0    2295    6504 
   Size:       2295    4209
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2544    2985    3197    3598    3971    5567    6056    6504 
   Size:       2544     441     212     401     373    1596     489     448

  Fragments arranged by size: 

                2544    1596     489     448     441     401     373     212
            
 
BbvCI CC'TCA_GC

Cuts at:      0      27    2783    6504 
   Size:         27    2756    3721

  Fragments arranged by size: 

                3721    2756      27
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5554    5588    6504 
   Size:       5554      34     916

  Fragments arranged by size: 

                5554     916      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3457    6365    6504 
   Size:       3457    2908     139

  Fragments arranged by size: 

                3457    2908     139
 
BclI T'GATC_A

Cuts at:      0    3650    6504 
   Size:       3650    2854
 
BfrBI ATG'CAT

Cuts at:      0    6244    6504 
   Size:       6244     260
 
BglII A'GATC_T

Cuts at:      0    4344    5412    6504 
   Size:       4344    1068    1092

  Fragments arranged by size: 

                4344    1092    1068
 
BlpI GC'TnA_GC

Cuts at:      0    2882    3201    6504 
   Size:       2882     319    3303

  Fragments arranged by size: 

                3303    2882     319
 
Bme1580I G_kGCm'C

Cuts at:      0    2066    3847    4489    5577    6504 
   Size:       2066    1781     642    1088     927

  Fragments arranged by size: 

                2066    1781    1088     927     642
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     217     409    1714    1790    2130    4531    6504 
   Size:        217     192    1305      76     340    2401    1973

  Fragments arranged by size: 

                2401    1973    1305     340     217     192      76
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     128    3602    3839    5024    5663    6504 
   Size:         29      99    3474     237    1185     639     841

  Fragments arranged by size: 

                3474    1185     841     639     237      99      29
 
Bpu10I CC'TnA_GC

Cuts at:      0      27     249     300     612    2783    5686    5939    6504 
   Size:         27     222      51     312    2171    2903     253     565

  Fragments arranged by size: 

                2903    2171     565     312     253     222      51      27
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750    2379    2657    3741    5252    5263    6082    6504 
   Size:        750    1629     278    1084    1511      11     819     422

  Fragments arranged by size: 

                1629    1511    1084     819     750     422     278      11
            
 
BsaHI Gr'CG_yC

Cuts at:      0    2292    2589    4149    6504 
   Size:       2292     297    1560    2355

  Fragments arranged by size: 

                2355    2292    1560     297
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3837    3867    4782    4812    5341    5371    6504 
   Size:       3837      30     915      30     529      30    1133

  Fragments arranged by size: 

                3837    1133     915     529      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      27    1505    2800    3415    3738    3896    4745    4790 
   Size:         27    1478    1295     615     323     158     849      45

Cuts at:   4790    6504 
   Size:       1714

  Fragments arranged by size: 

                1714    1478    1295     849     615     323     158      45
                  27
 
BseYI C'CCAG_C

Cuts at:      0     163     426    1025    4794    6504 
   Size:        163     263     599    3769    1710

  Fragments arranged by size: 

                3769    1710     599     263     163
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356    3310    5711    6504 
   Size:        356    2954    2401     793

  Fragments arranged by size: 

                2954    2401     793     356
 
BsiHKAI G_wGCw'C

Cuts at:      0    2883    4489    5577    6504 
   Size:       2883    1606    1088     927

  Fragments arranged by size: 

                2883    1606    1088     927
 
BsmI GAATG_Cn'

Cuts at:      0     255    1309    2474    5744    6504 
   Size:        255    1054    1165    3270     760

  Fragments arranged by size: 

                3270    1165    1054     760     255
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2580    4109    6504 
   Size:       2580    1529    2395

  Fragments arranged by size: 

                2580    2395    1529
 
Bsp1286I G_dGCh'C

Cuts at:      0    2066    2883    3847    4489    5577    5687    6504 
   Size:       2066     817     964     642    1088     110     817

  Fragments arranged by size: 

                2066    1088     964     817     817     642     110
 
BspHI T'CATG_A

Cuts at:      0     438    6234    6504 
   Size:        438    5796     270

  Fragments arranged by size: 

                5796     438     270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2151    5085    6504 
   Size:       2151    2934    1419

  Fragments arranged by size: 

                2934    2151    1419
 
BsrBI CCG'CTC

Cuts at:      0    3084    5643    6504 
   Size:       3084    2559     861

  Fragments arranged by size: 

                3084    2559     861
 
BsrDI GCAATG_nn'

Cuts at:      0     719    1565    2525    2625    4111    4560    5381    5933 
   Size:        719     846     960     100    1486     449     821     552

Cuts at:   5933    6504 
   Size:        571

  Fragments arranged by size: 

                1486     960     846     821     719     571     552     449
                 100
 
BsrFI r'CCGG_y

Cuts at:      0    4151    6504 
   Size:       4151    2353
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2317    2469    6504 
   Size:       2317     152    4035

  Fragments arranged by size: 

                4035    2317     152
 
BstEII G'GTnAC_C

Cuts at:      0      17    4012    6504 
   Size:         17    3995    2492

  Fragments arranged by size: 

                3995    2492      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1483    2940    4917    6504 
   Size:       1483    1457    1977    1587

  Fragments arranged by size: 

                1977    1587    1483    1457
 
BstYI r'GATC_y

Cuts at:      0    1282    4344    4807    5412    6504 
   Size:       1282    3062     463     605    1092

  Fragments arranged by size: 

                3062    1282    1092     605     463
 
Bsu36I CC'TnA_GG

Cuts at:      0    1040    6504 
   Size:       1040    5464
 
BtgI C'CryG_G

Cuts at:      0     587    6504 
   Size:        587    5917
 
BtsI GCAGTG_nn'

Cuts at:      0     792    2479    5577    5727    6504 
   Size:        792    1687    3098     150     777

  Fragments arranged by size: 

                3098    1687     792     777     150
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      93     123     128     158    6504 
   Size:         93      30       5      30    6346

  Fragments arranged by size: 

                6346      93      30      30       5
 
DraI TTT'AAA

Cuts at:      0    4307    6504 
   Size:       4307    2197
 
DraIII CAC_nnn'GTG

Cuts at:      0    4702    6504 
   Size:       4702    1802
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    4758    6000    6504 
   Size:       4758    1242     504

  Fragments arranged by size: 

                4758    1242     504
 
EaeI y'GGCC_r

Cuts at:      0    4131    6447    6504 
   Size:       4131    2316      57

  Fragments arranged by size: 

                4131    2316      57
 
EarI CTCTTCn'nnn_

Cuts at:      0    1339    1826    2205    2486    3821    4028    4862    5587 
   Size:       1339     487     379     281    1335     207     834     725

Cuts at:   5587    5660    6504 
   Size:         73     844

  Fragments arranged by size: 

                1339    1335     844     834     725     487     379     281
                 207      73
 
EcoICRI GAG'CTC

Cuts at:      0    2881    6504 
   Size:       2881    3623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29      62     128    1271    1372    1832    2009    2372 
   Size:         29      33      66    1143     101     460     177     363

Cuts at:   2372    2397    3602    3610    3617    3788    3839    4363    4843 
   Size:         25    1205       8       7     171      51     524     480

Cuts at:   4843    4876    5024    5051    5663    6062    6143    6504 
   Size:         33     148      27     612     399      81     361

  Fragments arranged by size: 

                1205    1143     612     524     480     460     399     363
                 361     177     171     148     101      81      66      51
                  33      33      29      27      25       8       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1354    4958    5401    6504 
   Size:       1354    3604     443    1103

  Fragments arranged by size: 

                3604    1354    1103     443
 
EcoO109I rG'GnC_Cy

Cuts at:      0    5175    6192    6504 
   Size:       5175    1017     312

  Fragments arranged by size: 

                5175    1017     312
 
EcoRI G'AATT_C

Cuts at:      0     404    2626    6504 
   Size:        404    2222    3878

  Fragments arranged by size: 

                3878    2222     404
 
EcoRV GAT'ATC

Cuts at:      0    2391    3990    6504 
   Size:       2391    1599    2514

  Fragments arranged by size: 

                2514    2391    1599
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     115    3010    3430    3818    5851    6173    6504 
   Size:        115    2895     420     388    2033     322     331

  Fragments arranged by size: 

                2895    2033     420     388     331     322     115
 
HaeII r_GCGC'y

Cuts at:      0    2295    6504 
   Size:       2295    4209
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1864    1879    1896    1911    2574    2606    3236    3268 
   Size:       1864      15      17      15     663      32     630      32

Cuts at:   3268    3697    3729    5341    5373    5461    5493    6037    6043 
   Size:        429      32    1612      32      88      32     544       6

Cuts at:   6043    6069    6075    6504 
   Size:         26       6     429

  Fragments arranged by size: 

                1864    1612     663     630     544     429     429      88
                  32      32      32      32      32      26      17      15
                  15       6       6
 
HincII GTy'rAC

Cuts at:      0     678    2602    4538    4736    6504 
   Size:        678    1924    1936     198    1768

  Fragments arranged by size: 

                1936    1924    1768     678     198
 
HindIII A'AGCT_T

Cuts at:      0    2704    3599    3629    4411    5183    6504 
   Size:       2704     895      30     782     772    1321

  Fragments arranged by size: 

                2704    1321     895     782     772      30
 
KasI G'GCGC_C

Cuts at:      0    2291    6504 
   Size:       2291    4213
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2572    5437    6504 
   Size:       2572    2865    1067

  Fragments arranged by size: 

                2865    2572    1067
 
MscI TGG'CCA

Cuts at:      0    4133    6449    6504 
   Size:       4133    2316      55

  Fragments arranged by size: 

                4133    2316      55
 
MslI CAynn'nnrTG

Cuts at:      0    1115    3286    4915    6239    6504 
   Size:       1115    2171    1629    1324     265

  Fragments arranged by size: 

                2171    1629    1324    1115     265
 
MspA1I CmG'CkG

Cuts at:      0     163    3433    5083    6504 
   Size:        163    3270    1650    1421

  Fragments arranged by size: 

                3270    1650    1421     163
 
NarI GG'CG_CC

Cuts at:      0    2292    6504 
   Size:       2292    4212
 
NcoI C'CATG_G

Cuts at:      0     587    6504 
   Size:        587    5917
 
NsiI A_TGCA'T

Cuts at:      0    6246    6504 
   Size:       6246     258
 
NspI r_CATG'y

Cuts at:      0    3198    3668    5581    6504 
   Size:       3198     470    1913     923

  Fragments arranged by size: 

                3198    1913     923     470
 
PciI A'CATG_T

Cuts at:      0    3194    6504 
   Size:       3194    3310
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     229     663    2191    2614    4471    6504 
   Size:        229     434    1528     423    1857    2033

  Fragments arranged by size: 

                2033    1857    1528     434     423     229
 
PfoI T'CCnGG_A

Cuts at:      0    2578    3240    3855    6504 
   Size:       2578     662     615    2649

  Fragments arranged by size: 

                2649    2578     662     615
 
PpuMI rG'GwC_Cy

Cuts at:      0    5175    6192    6504 
   Size:       5175    1017     312

  Fragments arranged by size: 

                5175    1017     312
 
PsiI TTA'TAA

Cuts at:      0    6290    6504 
   Size:       6290     214
 
PstI C_TGCA'G

Cuts at:      0    5082    6504 
   Size:       5082    1422
 
PvuII CAG'CTG

Cuts at:      0     163    3433    5083    6504 
   Size:        163    3270    1650    1421

  Fragments arranged by size: 

                3270    1650    1421     163
 
SacI G_AGCT'C

Cuts at:      0    2883    6504 
   Size:       2883    3621
 
SalI G'TCGA_C

Cuts at:      0    2600    6504 
   Size:       2600    3904
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1339    3821    4028    6504 
   Size:       1339    2482     207    2476

  Fragments arranged by size: 

                2482    2476    1339     207
 
SexAI A'CCwGG_T

Cuts at:      0     896    6504 
   Size:        896    5608
 
SfcI C'TryA_G

Cuts at:      0    4617    5078    6504 
   Size:       4617     461    1426

  Fragments arranged by size: 

                4617    1426     461
 
SfoI GGC'GCC

Cuts at:      0    2293    6504 
   Size:       2293    4211
 
SmlI C'TyrA_G

Cuts at:      0     765    2394    2672    3720    5267    5278    5336    6097 
   Size:        765    1629     278    1048    1547      11      58     761

Cuts at:   6097    6504 
   Size:        407

  Fragments arranged by size: 

                1629    1547    1048     765     761     407     278      58
                  11
 
SphI G_CATG'C

Cuts at:      0    3668    6504 
   Size:       3668    2836
 
SspI AAT'ATT

Cuts at:      0    3336    4932    6504 
   Size:       3336    1596    1572

  Fragments arranged by size: 

                3336    1596    1572
 
StyI C'CwwG_G

Cuts at:      0     587     700    3839    3927    6188    6504 
   Size:        587     113    3139      88    2261     316

  Fragments arranged by size: 

                3139    2261     587     316     113      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     236     306    1451    2422    3385    3726    3864    4472 
   Size:        236      70    1145     971     963     341     138     608

Cuts at:   4472    6504 
   Size:       2032

  Fragments arranged by size: 

                2032    1145     971     963     608     341     236     138
                  70
 
TatI w'GTAC_w

Cuts at:      0     561     604    1612    3262    3402    4079    6504 
   Size:        561      43    1008    1650     140     677    2425

  Fragments arranged by size: 

                2425    1650    1008     677     561     140      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3837    3869    6504 
   Size:       3837      32    2635

  Fragments arranged by size: 

                3837    2635      32
 
XbaI T'CTAG_A

Cuts at:      0     801    2892    5415    6378    6504 
   Size:        801    2091    2523     963     126

  Fragments arranged by size: 

                2523    2091     963     801     126
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     226     338    5912    6504 
   Size:        226     112    5574     592

  Fragments arranged by size: 

                5574     592     226     112
 
XhoI C'TCGA_G

Cuts at:      0    5336    6504 
   Size:       5336    1168
 
XmnI GAAnn'nnTTC

Cuts at:      0    1782    4515    5834    6504 
   Size:       1782    2733    1319     670

  Fragments arranged by size: 

                2733    1782    1319     670
 
ZraI GAC'GTC

Cuts at:      0    2590    6504 
   Size:       2590    3914
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AseI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NsiI     NspI     PciI    PflMI     PfoI    PpuMI     PsiI
     PstI    PvuII     SacI     SalI     SapI    SexAI     SfcI     SfoI
     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI     ApaI
     AscI    AsiSI    AvrII     BaeI    BamHI     BglI    BmgBI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BspEI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI     EagI
     EciI     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI  Tth111I     XmaI
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