Restriction map for
pF1KE3451
(Circular) (Six-base) MAPSORT of: pF1KE3451.seq Check: 1435 from: 1 to: 7076
pF1KE3451 7076 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 1996 1996
Size: 7076
AccI GT'mk_AC
Cuts at: 4114 4114
Size: 7076
Acc65I G'GTAC_C
Cuts at: 4092 4092
Size: 7076
AclI AA'CG_TT
Cuts at: 6916 6916
Size: 7076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1319 4901 5333 6080 6348 910
Size: 409 3582 432 747 268 1638
Fragments arranged by size:
3582 1638 747 432 409 268
AfeI AGC'GCT
Cuts at: 2973 2973
Size: 7076
AflII C'TTAA_G
Cuts at: 130 130
Size: 7076
AflIII A'CryG_T
Cuts at: 1646 4428 5532 6509 1646
Size: 2782 1104 977 2213
Fragments arranged by size:
2782 2213 1104 977
AgeI A'CCGG_T
Cuts at: 5464 5464
Size: 7076
AhdI GACnn_n'nnGTC
Cuts at: 774 774
Size: 7076
AleI CACnn'nnGTG
Cuts at: 1199 1199
Size: 7076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 517 3479 3619 517
Size: 2962 140 3974
Fragments arranged by size:
3974 2962 140
AlwNI CAG_nnn'CTG
Cuts at: 1147 1596 1657 1783 1914 1953 3372 3867 4381
Size: 449 61 126 131 39 1419 495 514
Cuts at: 4381 5948 1147
Size: 1567 2275
Fragments arranged by size:
2275 1567 1419 514 495 449 131 126
61 39
ApaI G_GGCC'C
Cuts at: 1258 3033 3111 3760 3951 1258
Size: 1775 78 649 191 4383
Fragments arranged by size:
4383 1775 649 191 78
ApaLI G'TGCA_C
Cuts at: 2899 5846 2899
Size: 2947 4129
ApoI r'AATT_y
Cuts at: 4080 6429 6685 4080
Size: 2349 256 4471
Fragments arranged by size:
4471 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7076
AvaI C'yCGr_G
Cuts at: 1231 1282 1819 1840 1892 2040 2799 3032 3113
Size: 51 537 21 52 148 759 233 81
Cuts at: 3113 3910 3926 3944 3973 4096 1231
Size: 797 16 18 29 123 4211
Fragments arranged by size:
4211 797 759 537 233 148 123 81
52 51 29 21 18 16
BamHI G'GATC_C
Cuts at: 4101 4101
Size: 7076
BanI G'GyrC_C
Cuts at: 478 1048 1695 1801 2106 2515 2686 2761 3961
Size: 570 647 106 305 409 171 75 1200
Cuts at: 3961 4092 4506 4754 4789 478
Size: 131 414 248 35 2765
Fragments arranged by size:
2765 1200 647 570 414 409 305 248
171 131 106 75 35
BanII G_rGCy'C
Cuts at: 1258 1763 1790 1820 1880 2605 2792 3033 3094
Size: 505 27 30 60 725 187 241 61
Cuts at: 3094 3111 3307 3314 3357 3493 3760 3771 3886
Size: 17 196 7 43 136 267 11 115
Cuts at: 3886 3951 3982 4090 5120 1258
Size: 65 31 108 1030 3214
Fragments arranged by size:
3214 1030 725 505 267 241 196 187
136 115 108 65 61 60 43 31
30 27 17 11 7
BbeI G_GCGC'C
Cuts at: 2110 4510 4758 2110
Size: 2400 248 4428
Fragments arranged by size:
4428 2400 248
BbsI GAAGACnn'nnnn_
Cuts at: 2927 2927
Size: 7076
BbvCI CC'TCA_GC
Cuts at: 1706 2708 1706
Size: 1002 6074
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7042
BciVI GTATCCnnnnn_n'
Cuts at: 3283 4974 5735 7076 3283
Size: 1691 761 1341 3283
Fragments arranged by size:
3283 1691 1341 761
BclI T'GATC_A
Cuts at: 857 971 4596 857
Size: 114 3625 3337
Fragments arranged by size:
3625 3337 114
BglI GCCn_nnn'nGGC
Cuts at: 1236 2572 2665 2802 3430 3587 1236
Size: 1336 93 137 628 157 4725
Fragments arranged by size:
4725 1336 628 157 137 93
BglII A'GATC_T
Cuts at: 248 518 4591 6628 248
Size: 270 4073 2037 696
Fragments arranged by size:
4073 2037 696 270
BlpI GC'TnA_GC
Cuts at: 490 1784 1966 4013 4188 490
Size: 1294 182 2047 175 3378
Fragments arranged by size:
3378 2047 1294 182 175
Bme1580I G_kGCm'C
Cuts at: 1258 1419 1804 2520 2689 2903 3033 3111 3760
Size: 161 385 716 169 214 130 78 649
Cuts at: 3760 3951 4701 4794 5850 1258
Size: 191 750 93 1056 2484
Fragments arranged by size:
2484 1056 750 716 649 385 214 191
169 161 130 93 78
BmgBI CAC'GTC
Cuts at: 1647 1647
Size: 7076
BmrI ACTGGGnnnn_n'
Cuts at: 1044 1253 1402 1435 4288 4369 4704 6848 1044
Size: 209 149 33 2853 81 335 2144 1272
Fragments arranged by size:
2853 2144 1272 335 209 149 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2619 3018 3495 3903 4021 5443 2619
Size: 399 477 408 118 1422 4252
Fragments arranged by size:
4252 1422 477 408 399 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 843 1760 2454 2608 3013 3064 3217 3429
Size: 425 917 694 154 405 51 153 212
Cuts at: 3429 3667 3991 418
Size: 238 324 3503
Fragments arranged by size:
3503 917 694 425 405 324 238 212
154 153 51
Bpu10I CC'TnA_GC
Cuts at: 1675 1706 2708 1675
Size: 31 1002 6043
Fragments arranged by size:
6043 1002 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 313 334 1747 2764 4252 5131 5623 5921
Size: 36 21 1413 1017 1488 879 492 298
Cuts at: 5921 277
Size: 1432
Fragments arranged by size:
1488 1432 1413 1017 879 492 298 36
21
BsaI GGTCTCn'nnnn_
Cuts at: 2171 3466 6780 2171
Size: 1295 3314 2467
Fragments arranged by size:
3314 2467 1295
BsaAI yAC'GTr
Cuts at: 192 4429 192
Size: 4237 2839
BsaBI GATnn'nnATC
Cuts at: 6 1345 4590 6627 6
Size: 1339 3245 2037 455
Fragments arranged by size:
3245 2037 1339 455
BsaHI Gr'CG_yC
Cuts at: 1138 1993 2107 3237 3694 3932 4507 4755 5457
Size: 855 114 1130 457 238 575 248 702
Cuts at: 5457 6953 1138
Size: 1496 1261
Fragments arranged by size:
1496 1261 1130 855 702 575 457 248
238 114
BsaWI w'CCGG_w
Cuts at: 878 1325 1337 1460 2222 2501 2938 4265 4786
Size: 447 12 123 762 279 437 1327 521
Cuts at: 4786 5464 5738 5885 6296 878
Size: 678 274 147 411 1658
Fragments arranged by size:
1658 1327 762 678 521 447 437 411
279 274 147 123 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1324 1384 1542 1566 1723 1726 1828 1837 1858
Size: 60 158 24 157 3 102 9 21
Cuts at: 1858 1936 2326 2329 2332 2335 2380 2395 2398
Size: 78 390 3 3 3 45 15 3
Cuts at: 2398 2410 2413 2416 2425 2428 2431 2505 2611
Size: 12 3 3 9 3 3 74 106
Cuts at: 2611 2696 2856 2977 3037 3307 3432 3466 3511
Size: 85 160 121 60 270 125 34 45
Cuts at: 3511 1324
Size: 4889
Fragments arranged by size:
4889 390 270 160 158 157 125 121
106 102 85 78 74 60 60 45
45 34 24 21 15 12 9 9
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 308 507 1088 1251 1399 1789 1866 1946 1961
Size: 199 581 163 148 390 77 80 15
Cuts at: 1961 2451 2723 2817 2974 2985 3617 3651 3789
Size: 490 272 94 157 11 632 34 138
Cuts at: 3789 3950 4060 4501 5259 5836 6332 308
Size: 161 110 441 758 577 496 1052
Fragments arranged by size:
1052 758 632 581 577 496 490 441
390 272 199 163 161 157 148 138
110 94 80 77 34 15 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 544 2020 399
Size: 145 1476 5455
Fragments arranged by size:
5455 1476 145
BsiEI CG_ry'CG
Cuts at: 104 125 3361 4618 4664 5872 104
Size: 21 3236 1257 46 1208 1308
Fragments arranged by size:
3236 1308 1257 1208 46 21
BsiHKAI G_wGCw'C
Cuts at: 350 496 878 1075 1196 1763 2497 2903 4090
Size: 146 382 197 121 567 734 406 1187
Cuts at: 4090 4868 5058 5850 350
Size: 778 190 792 1576
Fragments arranged by size:
1576 1187 792 778 734 567 406 382
197 190 146 121
BsmI GAATG_Cn'
Cuts at: 1218 1218
Size: 7076
BsmBI CGTCTCn'nnnn_
Cuts at: 1747 1984 1747
Size: 237 6839
Bsp1286I G_dGCh'C
Cuts at: 350 496 878 1075 1196 1258 1419 1763 1790
Size: 146 382 197 121 62 161 344 27
Cuts at: 1790 1804 1820 1880 2497 2520 2605 2689 2792
Size: 14 16 60 617 23 85 84 103
Cuts at: 2792 2903 3033 3094 3111 3307 3314 3357 3493
Size: 111 130 61 17 196 7 43 136
Cuts at: 3493 3760 3771 3886 3951 3982 4090 4701 4794
Size: 267 11 115 65 31 108 611 93
Cuts at: 4794 4868 5058 5120 5850 350
Size: 74 190 62 730 1576
Fragments arranged by size:
1576 730 617 611 382 344 267 197
196 190 161 146 136 130 121 115
111 108 103 93 85 84 74 65
62 62 61 60 43 31 27 23
17 16 14 11 7
BspEI T'CCGG_A
Cuts at: 1337 2222 6296 1337
Size: 885 4074 2117
Fragments arranged by size:
4074 2117 885
BspHI T'CATG_A
Cuts at: 6252 6252
Size: 7076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 312 371 464 1163 1937 4126 4340 4642
Size: 37 59 93 699 774 2189 214 302
Cuts at: 4642 5023 275
Size: 381 2328
Fragments arranged by size:
2328 2189 774 699 381 302 214 93
59 37
BsrBI CCG'CTC
Cuts at: 50 1691 1857 2718 3339 3545 5368 5422 6906
Size: 1641 166 861 621 206 1823 54 1484
Cuts at: 6906 7071 50
Size: 165 55
Fragments arranged by size:
1823 1641 1484 861 621 206 166 165
55 54
BsrDI GCAATG_nn'
Cuts at: 499 929 1010 2903 3843 4988 499
Size: 430 81 1893 940 1145 2587
Fragments arranged by size:
2587 1893 1145 940 430 81
BsrFI r'CCGG_y
Cuts at: 180 3519 3939 5074 5255 5464 180
Size: 3339 420 1135 181 209 1792
Fragments arranged by size:
3339 1792 1135 420 209 181
BssHII G'CGCG_C
Cuts at: 3832 4017 3832
Size: 185 6891
BssSI C'ACGA_G
Cuts at: 1068 2135 5347 5705 1068
Size: 1067 3212 358 2439
Fragments arranged by size:
3212 2439 1067 358
BstAPI GCAn_nnn'nTGC
Cuts at: 882 1938 2247 4453 882
Size: 1056 309 2206 3505
Fragments arranged by size:
3505 2206 1056 309
BstBI TT'CG_AA
Cuts at: 5437 5437
Size: 7076
BstXI CCAn_nnnn'nTGG
Cuts at: 192 1978 2532 2613 3958 192
Size: 1786 554 81 1345 3310
Fragments arranged by size:
3310 1786 1345 554 81
BstYI r'GATC_y
Cuts at: 1 248 518 1542 3634 4101 4568 4591 4926
Size: 247 270 1024 2092 467 467 23 335
Cuts at: 4926 5172 6184 6270 6282 6628 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2092 1024 1012 467 467 449 346 335
270 247 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1292 2288 2594 3315 3426 1292
Size: 996 306 721 111 4942
Fragments arranged by size:
4942 996 721 306 111
BtgI C'CryG_G
Cuts at: 260 590 665 1496 1805 2102 2570 2729 3639
Size: 330 75 831 309 297 468 159 910
Cuts at: 3639 3747 260
Size: 108 3589
Fragments arranged by size:
3589 910 831 468 330 309 297 159
108 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1991 5026 5207 5370 1991
Size: 3035 181 163 3697
Fragments arranged by size:
3697 3035 181 163
BtsI GCAGTG_nn'
Cuts at: 118 812 1834 2481 2654 3145 3710 4319 118
Size: 694 1022 647 173 491 565 609 2875
Fragments arranged by size:
2875 1022 694 647 609 565 491 173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 132 167 132
Size: 35 7041
DraI TTT'AAA
Cuts at: 4075 4075
Size: 7076
DraIII CAC_nnn'GTG
Cuts at: 549 549
Size: 7076
DrdI GACnn_nn'nnGTC
Cuts at: 4782 5640 4782
Size: 858 6218
EaeI y'GGCC_r
Cuts at: 122 182 341 449 695 1635 1831 1980 2573
Size: 60 159 108 246 940 196 149 593
Cuts at: 2573 3134 3218 3329 3358 3707 3744 4661 4835
Size: 561 84 111 29 349 37 917 174
Cuts at: 4835 5226 5253 6598 122
Size: 391 27 1345 600
Fragments arranged by size:
1345 940 917 600 593 561 391 349
246 196 174 159 149 111 108 84
60 37 29 27
EagI C'GGCC_G
Cuts at: 122 3358 4661 122
Size: 3236 1303 2537
Fragments arranged by size:
3236 2537 1303
EarI CTCTTCn'nnn_
Cuts at: 787 922 1847 2303 2330 2339 2357 2366 2372
Size: 135 925 456 27 9 18 9 6
Cuts at: 2372 2387 2402 3388 3535 5099 5309 787
Size: 15 15 986 147 1564 210 2554
Fragments arranged by size:
2554 1564 986 925 456 210 147 135
27 18 15 15 9 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1516 1933 3715 3816 5284 5594 5740 6886 1516
Size: 417 1782 101 1468 310 146 1146 1706
Fragments arranged by size:
1782 1706 1468 1146 417 310 146 101
EcoICRI GAG'CTC
Cuts at: 1761 4088 1761
Size: 2327 4749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 843 910 1319 1760 2454 2608 3013 3064
Size: 425 67 409 441 694 154 405 51
Cuts at: 3064 3217 3429 3667 3991 4901 5333 6080 6348
Size: 153 212 238 324 910 432 747 268
Cuts at: 6348 418
Size: 1146
Fragments arranged by size:
1146 910 747 694 441 432 425 409
405 324 268 238 212 154 153 67
51
EcoNI CCTnn'n_nnAGG
Cuts at: 2810 3534 2810
Size: 724 6352
EcoO109I rG'GnC_Cy
Cuts at: 324 1152 1238 1255 1288 1395 1671 1926 2044
Size: 828 86 17 33 107 276 255 118
Cuts at: 2044 2265 2455 2583 2803 2891 3029 3107 3108
Size: 221 190 128 220 88 138 78 1
Cuts at: 3108 3369 3431 3785 3915 3991 4215 324
Size: 261 62 354 130 76 224 3185
Fragments arranged by size:
3185 828 354 276 261 255 224 221
220 190 138 130 128 118 107 88
86 78 76 62 33 17 1
EcoRI G'AATT_C
Cuts at: 4080 4080
Size: 7076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 5095 537
Size: 4558 2518
FspI TGC'GCA
Cuts at: 4857 4857
Size: 7076
HaeII r_GCGC'y
Cuts at: 1915 2110 2975 3017 3040 3462 3796 4510 4758
Size: 195 865 42 23 422 334 714 248
Cuts at: 4758 5780 6759 1915
Size: 1022 979 2232
Fragments arranged by size:
2232 1022 979 865 714 422 334 248
195 42 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 1516 1548 3289 3321 4918 4948 4950
Size: 32 724 32 1741 32 1597 30 2
Cuts at: 4950 4980 760
Size: 30 2856
Fragments arranged by size:
2856 1741 1597 724 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1323 4115 1323
Size: 2792 4284
KasI G'GCGC_C
Cuts at: 2106 4506 4754 2106
Size: 2400 248 4428
Fragments arranged by size:
4428 2400 248
KpnI G_GTAC'C
Cuts at: 4096 4096
Size: 7076
MluI A'CGCG_T
Cuts at: 6509 6509
Size: 7076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2048 5747 5931 2048
Size: 3699 184 3193
Fragments arranged by size:
3699 3193 184
MscI TGG'CCA
Cuts at: 3136 3220 4837 3136
Size: 84 1617 5375
Fragments arranged by size:
5375 1617 84
MslI CAynn'nnrTG
Cuts at: 793 979 1199 1645 3601 5065 5192 793
Size: 186 220 446 1956 1464 127 2677
Fragments arranged by size:
2677 1956 1464 446 220 186 127
MspA1I CmG'CkG
Cuts at: 816 1701 1965 2428 2742 3195 3526 3687 3749
Size: 885 264 463 314 453 331 161 62
Cuts at: 3749 4187 4501 4861 5874 6119 816
Size: 438 314 360 1013 245 1773
Fragments arranged by size:
1773 1013 885 463 453 438 360 331
314 314 264 245 161 62
NaeI GCC'GGC
Cuts at: 182 3521 3941 5257 182
Size: 3339 420 1316 2001
Fragments arranged by size:
3339 2001 1316 420
NarI GG'CG_CC
Cuts at: 2107 4507 4755 2107
Size: 2400 248 4428
Fragments arranged by size:
4428 2400 248
NcoI C'CATG_G
Cuts at: 260 590 2570 3639 260
Size: 330 1980 1069 3697
Fragments arranged by size:
3697 1980 1069 330
NgoMIV G'CCGG_C
Cuts at: 180 3519 3939 5255 180
Size: 3339 420 1316 2001
Fragments arranged by size:
3339 2001 1316 420
NotI GC'GGCC_GC
Cuts at: 122 122
Size: 7076
NspI r_CATG'y
Cuts at: 792 900 1908 2247 3600 4129 4629 5536 792
Size: 108 1008 339 1353 529 500 907 2332
Fragments arranged by size:
2332 1353 1008 907 529 500 339 108
PasI CC'CwG_GG
Cuts at: 3700 3760 3700
Size: 60 7016
PciI A'CATG_T
Cuts at: 5532 5532
Size: 7076
PflMI CCAn_nnn'nTGG
Cuts at: 549 1147 549
Size: 598 6478
PfoI T'CCnGG_A
Cuts at: 920 1160 920
Size: 240 6836
PmeI GTTT'AAAC
Cuts at: 4075 4075
Size: 7076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 698 730 6215 6247 698
Size: 32 5485 32 1527
Fragments arranged by size:
5485 1527 32 32
PpuMI rG'GwC_Cy
Cuts at: 324 1152 1288 1395 1926 2044 2265 2583 2891
Size: 828 136 107 531 118 221 318 308
Cuts at: 2891 3369 3915 3991 324
Size: 478 546 76 3409
Fragments arranged by size:
3409 828 546 531 478 318 308 221
136 118 107 76
PshAI GACnn'nnGTC
Cuts at: 1318 1318
Size: 7076
PspOMI G'GGCC_C
Cuts at: 1254 3029 3107 3756 3947 1254
Size: 1775 78 649 191 4383
Fragments arranged by size:
4383 1775 649 191 78
PstI C_TGCA'G
Cuts at: 1934 2427 3692 4123 4808 1934
Size: 493 1265 431 685 4202
Fragments arranged by size:
4202 1265 685 493 431
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7076
PvuII CAG'CTG
Cuts at: 816 1965 2742 3687 4501 4861 816
Size: 1149 777 945 814 360 3031
Fragments arranged by size:
3031 1149 945 814 777 360
RsrII CG'GwC_CG
Cuts at: 5271 5271
Size: 7076
SacI G_AGCT'C
Cuts at: 1763 4090 1763
Size: 2327 4749
SacII CC_GC'GG
Cuts at: 3750 3750
Size: 7076
SalI G'TCGA_C
Cuts at: 4113 4113
Size: 7076
SanDI GG'GwC_CC
Cuts at: 1395 2044 3915 1395
Size: 649 1871 4556
Fragments arranged by size:
4556 1871 649
SapI GCTCTTCn'nnn_
Cuts at: 1847 3535 5099 5309 1847
Size: 1688 1564 210 3614
Fragments arranged by size:
3614 1688 1564 210
SbfI CC_TGCA'GG
Cuts at: 4123 4123
Size: 7076
ScaI AGT'ACT
Cuts at: 6593 6593
Size: 7076
SfcI C'TryA_G
Cuts at: 33 612 1183 1930 2423 3688 4119 4804 5797
Size: 579 571 747 493 1265 431 685 993
Cuts at: 5797 5988 33
Size: 191 1121
Fragments arranged by size:
1265 1121 993 747 685 579 571 493
431 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2572 3430 2572
Size: 858 6218
SfoI GGC'GCC
Cuts at: 2108 4508 4756 2108
Size: 2400 248 4428
Fragments arranged by size:
4428 2400 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7076
SmaI CCC'GGG
Cuts at: 1233 1284 1894 2801 3115 3912 3928 3946 3975
Size: 51 610 907 314 797 16 18 29
Cuts at: 3975 4098 1233
Size: 123 4211
Fragments arranged by size:
4211 907 797 610 314 123 51 29
18 16
SmlI C'TyrA_G
Cuts at: 130 292 328 349 1762 2779 4231 5146 5638
Size: 162 36 21 1413 1017 1452 915 492
Cuts at: 5638 5900 130
Size: 262 1306
Fragments arranged by size:
1452 1413 1306 1017 915 492 262 162
36 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7076
SphI G_CATG'C
Cuts at: 1908 3600 4129 4629 1908
Size: 1692 529 500 4355
Fragments arranged by size:
4355 1692 529 500
SrfI GCCC'GGGC
Cuts at: 3946 3946
Size: 7076
StuI AGG'CCT
Cuts at: 1027 1570 1027
Size: 543 6533
StyI C'CwwG_G
Cuts at: 260 590 2438 2570 3639 4210 4564 260
Size: 330 1848 132 1069 571 354 2772
Fragments arranged by size:
2772 1848 1069 571 354 330 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1637 4659 4792 5251 5460 1637
Size: 3022 133 459 209 3253
Fragments arranged by size:
3253 3022 459 209 133
TatI w'GTAC_w
Cuts at: 653 6591 653
Size: 5938 1138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 361 393 6593 6625 361
Size: 32 6200 32 812
Fragments arranged by size:
6200 812 32 32
Tth111I GACn'n_nGTC
Cuts at: 4873 4873
Size: 7076
XbaI T'CTAG_A
Cuts at: 4107 4107
Size: 7076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 504 546 1122 1638 4571 282
Size: 222 42 576 516 2933 2787
Fragments arranged by size:
2933 2787 576 516 222 42
XmaI C'CCGG_G
Cuts at: 1231 1282 1892 2799 3113 3910 3926 3944 3973
Size: 51 610 907 314 797 16 18 29
Cuts at: 3973 4096 1231
Size: 123 4211
Fragments arranged by size:
4211 907 797 610 314 123 51 29
18 16
ZraI GAC'GTC
Cuts at: 1994 1994
Size: 7076
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BmtI BsaXI
BsiWI BsrGI BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PmlI PsiI PspXI PsrI SexAI SgrAI SnaBI SspI
SwaI XhoI XmnI