Restriction map for pF1KB4231
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspHI       AspI        AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsiHKAI     
BsiWI       BsmBI       BspMI       BsrBI       BsrFI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      CpoI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     FauI        FseI        HaeII       HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
RsrII       SacI        SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       Sse8387I    StuI        SwaI        Tth111I     
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccIII       5204  TCCGGA               T^CCGGA
AcyI         2859  GACGCC               G[GA]^CG[CT]C
AflII        3708  CTTAAG               C^TTAAG
ApaI          417  GGGCCC               GGGCC^C
AvaI           38  CCCGGG               C^[CT]CG[GA]G
BfrI         3708  CTTAAG               C^TTAAG
BlpI         4447  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4447  GCTCAGC              GC^T[ACGT]AGC
BsaHI        2859  GACGCC               G[GA]^CG[CT]C
BseAI        5204  TCCGGA               T^CCGGA
BsiEI        2391  CGATCG               CG[GA][CT]^CG
BsoBI          38  CCCGGG               C^[CT]CG[GA]G
BspEI        5204  TCCGGA               T^CCGGA
BssSI         715  CACGAG               CACGAG(-5/-1)
BstXI        5171  CCAGCAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        4447  GCTCAGC              GC^T[ACGT]AGC
DraIII       1229  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4894  GACAACATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1121  GAATTC               G^AATTC
Hin1I        2859  GACGCC               G[GA]^CG[CT]C
HindIII       104  AAGCTT               A^AGCTT
MaeII        4109  ACGT                 A^CGT
MroI         5204  TCCGGA               T^CCGGA
PvuI         2391  CGATCG               CGAT^CG
SexAI        1230  ACCTGGT              A^CC[AT]GGT
SmaI           38  CCCGGG               CCC^GGG
SpeI         1743  ACTAGT               A^CTAGT
XmaCI          38  CCCGGG               C^CCGGG
XmaI           38  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII         26  ACATGT               A^C[GA][CT]GT
              529  ACATGT              
AlwNI         839  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1648  CAGACACTG           
AocI         1821  CCTGAGG              CC^T[ACGT]AGG
             4072  CCTCAGG             
AviII          98  TGCGCA               TGC^GCA
             5192  TGCGCA              
BalI         2753  TGGCCA               TGG^CCA
             4245  TGGCCA              
BanI         2179  GGCACC               G^G[CT][GA]CC
             2799  GGCACC              
BanII          41  GGGCTC               G[GA]GC[CT]^C
              417  GGGCCC              
BciVI        2463  GGATAC               GTATCC(6/5)
             4686  GGATAC              
BsaWI        3037  TCCGGT               [AT]^CCGG[AT]
             5204  TCCGGA              
BseRI         158  CTCCTC               GAGGAG(10/8)
             5242  GAGGAG              
Bsp1407I      984  TGTACA               T^GTACA
             5210  TGTACA              
BspDI        2389  ATCGAT               AT^CGAT
             2393  ATCGAT              
BspLU11I       26  ACATGT               A^CATGT
              529  ACATGT              
BsrGI         984  TGTACA               T^GTACA
             5210  TGTACA              
BstAPI        101  GCAAAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4372  GCACTTTATGC         
Bsu36I       1821  CCTGAGG              CC^T[ACGT]AGG
             4072  CCTCAGG             
ClaI         2389  ATCGAT               AT^CGAT
             2393  ATCGAT              
Eco81I       1821  CCTGAGG              CC^T[ACGT]AGG
             4072  CCTCAGG             
FspI           98  TGCGCA               TGC^GCA
             5192  TGCGCA              
HgaI         2859  GACGC                GACGC(5/10)
             4774  GCGTC               
MluNI        2753  TGGCCA               TGG^CCA
             4245  TGGCCA              
MscI         2753  TGGCCA               TGG^CCA
             4245  TGGCCA              
ScaI          236  AGTACT               AGT^ACT
             4413  AGTACT              
SphI          259  GCATGC               GCATG^C
             2959  GCATGC              
SspBI         984  TGTACA               T^GTACA
             5210  TGTACA              
SspI         1105  AATATT               AAT^ATT
             3381  AATATT              
XbaI          297  TCTAGA               T^CTAGA
             2352  TCTAGA              
XcmI         1908  CCAGACAACTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2840  CCATGTTTAAGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        ApoI        AseI        AsnI        
Asp700I     AvaII       BbsI        BbvI        BclI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoRV       EcoT14I     
EcoT22I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PpuMI       PshBI       PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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