Restriction map for pF1KE5841
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BbsI        BcgI        BfrI        
BlnI        BpuAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BsrGI       
BssHII      BstBI       Bsu36I      ClaI        CpoI        
DraI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
FseI        HincII      HindII      HpaI        KpnI        
Ksp632I     KspI        MfeI        MluI        MunI        
NheI        NotI        NruI        NspV        PacI        
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SwaI        Tth111I     XbaI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccIII       4688  TCCGGA               T^CCGGA
BbrPI         947  CACGTG               CAC^GTG
BciVI         386  GTATCC               GTATCC(6/5)
BglII        5375  AGATCT               A^GATCT
BsaBI         976  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4688  TCCGGA               [AT]^CCGG[AT]
BseAI        4688  TCCGGA               T^CCGGA
BspEI        4688  TCCGGA               T^CCGGA
BspLU11I     1326  ACATGT               A^CATGT
Bst1107I     1084  GTATAC               GTA^TAC
BstZ17I      1084  GTATAC               GTA^TAC
DrdI         3095  GACAGAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      3572  ATGCAT               ATGCA^T
HindIII      2897  AAGCTT               A^AGCTT
MamI          976  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         4688  TCCGGA               T^CCGGA
MseI         3920  TTAA                 T^TAA
NdeI         2756  CATATG               CA^TATG
NsiI         3572  ATGCAT               ATGCA^T
PaeR7I       3835  CTCGAG               C^TCGAG
PmaCI         947  CACGTG               CAC^GTG
PmlI          947  CACGTG               CAC^GTG
PshAI        1255  GACGGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI          243  CCTGCAGG             CCTGCA^GG
ScaI          806  AGTACT               AGT^ACT
SexAI        4919  ACCAGGT              A^CC[AT]GGT
SmaI         1762  CCCGGG               CCC^GGG
Sse8387I      243  CCTGCAGG             CCTGCA^GG
SspI         2840  AATATT               AAT^ATT
StuI         2288  AGGCCT               AGG^CCT
Tru9I        3920  TTAA                 T^TAA
XhoI         3835  CTCGAG               C^TCGAG
XmaCI        1762  CCCGGG               C^CCGGG
XmaI         1762  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          752  GTCTAC               GT^[AC][GT]AC
             1084  GTATAC              
AccII        4304  CGCG                 CG^CG
             5107  CGCG                
Alw44I        875  GTGCAC               G^TGCAC
              916  GTGCAC              
ApaLI         875  GTGCAC               G^TGCAC
              916  GTGCAC              
Asp700I       258  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4557  GAAGGACTTC          
AviII        2293  TGCGCA               TGC^GCA
             5339  TGCGCA              
BclI         2691  TGATCA               T^GATCA
             5121  TGATCA              
BsaI         3401  GAGACC               GGTCTC(1/5)
             1299  GGTCTC              
BssSI        2503  CACGAG               CACGAG(-5/-1)
             3440  CTCGTG              
BstEII        396  GGTCACC              G^GT[ACGT]ACC
             1755  GGTGACC             
BstPI         396  GGTCACC              G^GT[ACGT]ACC
             1755  GGTGACC             
BstUI        4304  CGCG                 CG^CG
             5107  CGCG                
EcoO65I       396  GGTCACC              G^GT[ACGT]ACC
             1755  GGTGACC             
FbaI         2691  TGATCA               T^GATCA
             5121  TGATCA              
FspI         2293  TGCGCA               TGC^GCA
             5339  TGCGCA              
MvnI         4304  CGCG                 CG^CG
             5107  CGCG                
SfiI          205  GGCCTGAATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1522  GGCCACTGGGGCC       
XmnI          258  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4557  GAAGGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
SphI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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