Restriction map for pF1KE5841
 (Linear) (Six-base) MAPSORT of: KIEE5841.seq  Check: 6674  from: 1  to: 5394



KIEE5841.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     251    1650    1719    3325    4930    4957    5358    5394 
   Size:        251    1399      69    1606    1605      27     401      36

  Fragments arranged by size: 

                1606    1605    1399     401     251      69      36      27
            
 
AccI GT'mk_AC

Cuts at:      0     753    1085    5394 
   Size:        753     332    4309

  Fragments arranged by size: 

                4309     753     332
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     659    2098    2810    4625    5394 
   Size:        659    1439     712    1815     769

  Fragments arranged by size: 

                1815    1439     769     712     659
 
AflIII A'CryG_T

Cuts at:      0     758     946    1326    5394 
   Size:        758     188     380    4068

  Fragments arranged by size: 

                4068     758     380     188
 
AleI CACnn'nnGTG

Cuts at:      0    3347    3459    4615    5394 
   Size:       3347     112    1156     779

  Fragments arranged by size: 

                3347    1156     779     112
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2027    2670    3437    5006    5394 
   Size:       2027     643     767    1569     388

  Fragments arranged by size: 

                2027    1569     767     643     388
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39     714    1435    1821    1939    2406    2525    3692 
   Size:         39     675     721     386     118     467     119    1167

Cuts at:   3692    4662    4758    4888    4914    5394 
   Size:        970      96     130      26     480

  Fragments arranged by size: 

                1167     970     721     675     480     467     386     130
                 119     118      96      39      26
 
ApaI G_GGCC'C

Cuts at:      0    3220    4368    4473    5394 
   Size:       3220    1148     105     921

  Fragments arranged by size: 

                3220    1148     921     105
 
ApaLI G'TGCA_C

Cuts at:      0     875     916    5394 
   Size:        875      41    4478

  Fragments arranged by size: 

                4478     875      41
 
AvaI C'yCGr_G

Cuts at:      0    1721    1762    3835    5213    5394 
   Size:       1721      41    2073    1378     181

  Fragments arranged by size: 

                2073    1721    1378     181      41
 
BamHI G'GATC_C

Cuts at:      0    1206    1465    1590    2985    5394 
   Size:       1206     259     125    1395    2409

  Fragments arranged by size: 

                2409    1395    1206     259     125
 
BanI G'GyrC_C

Cuts at:      0     281     891    1148    1971    2262    2335    2429    2563 
   Size:        281     610     257     823     291      73      94     134

Cuts at:   2563    2945    3673    3736    3982    4233    4273    4867    5269 
   Size:        382     728      63     246     251      40     594     402

Cuts at:   5269    5394 
   Size:        125

  Fragments arranged by size: 

                 823     728     610     594     402     382     291     281
                 257     251     246     134     125      94      73      63
                  40
 
BanII G_rGCy'C

Cuts at:      0    1163    1376    1886    3220    3392    4368    4473    4628 
   Size:       1163     213     510    1334     172     976     105     155

Cuts at:   4628    4963    4980    5394 
   Size:        335      17     414

  Fragments arranged by size: 

                1334    1163     976     510     414     335     213     172
                 155     105      17
 
BbeI G_GCGC'C

Cuts at:      0     285    1152    2433    5394 
   Size:        285     867    1281    2961

  Fragments arranged by size: 

                2961    1281     867     285
 
BbvCI CC'TCA_GC

Cuts at:      0     905    1007    1712    3750    4047    4318    4420    4790 
   Size:        905     102     705    2038     297     271     102     370

Cuts at:   4790    5394 
   Size:        604

  Fragments arranged by size: 

                2038     905     705     604     370     297     271     102
                 102
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     397    5394 
   Size:        397    4997
 
BclI T'GATC_A

Cuts at:      0    2691    5121    5394 
   Size:       2691    2430     273

  Fragments arranged by size: 

                2691    2430     273
 
BfrBI ATG'CAT

Cuts at:      0    3574    5394 
   Size:       3574    1820
 
BglI GCCn_nnn'nGGC

Cuts at:      0      91     212     889    1361    1520    1529    2477    2597 
   Size:         91     121     677     472     159       9     948     120

Cuts at:   2597    2873    3353    4846    5089    5394 
   Size:        276     480    1493     243     305

  Fragments arranged by size: 

                1493     948     677     480     472     305     276     243
                 159     121     120      91       9
 
BglII A'GATC_T

Cuts at:      0    5375    5394 
   Size:       5375      19
 
BlpI GC'TnA_GC

Cuts at:      0      70     492    1375    4646    5093    5150    5394 
   Size:         70     422     883    3271     447      57     244

  Fragments arranged by size: 

                3271     883     447     422     244      70      57
 
Bme1580I G_kGCm'C

Cuts at:      0     879     894     920    1055    1339    1429    1612    1976 
   Size:        879      15      26     135     284      90     183     364

Cuts at:   1976    2106    2267    2340    2451    2514    2907    2915    3060 
   Size:        130     161      73     111      63     393       8     145

Cuts at:   3060    3133    3151    3196    3220    3240    3531    3741    4167 
   Size:         73      18      45      24      20     291     210     426

Cuts at:   4167    4236    4368    4398    4473    4851    4956    5073    5085 
   Size:         69     132      30      75     378     105     117      12

Cuts at:   5085    5295    5394 
   Size:        210      99

  Fragments arranged by size: 

                 879     426     393     378     364     291     284     210
                 210     183     161     145     135     132     130     117
                 111     105      99      90      75      73      73      69
                  63      45      30      26      24      20      18      15
                  12       8
 
BmgBI CAC'GTC

Cuts at:      0    1498    1575    5394 
   Size:       1498      77    3819

  Fragments arranged by size: 

                3819    1498      77
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      67     597    1300    1536    1590    3405    4366    5307 
   Size:         67     530     703     236      54    1815     961     941

Cuts at:   5307    5394 
   Size:         87

  Fragments arranged by size: 

                1815     961     941     703     530     236      87      67
                  54
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27    5394 
   Size:         27    5367
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436    1843    2233    2438    3919    4852    5020    5317 
   Size:        436    1407     390     205    1481     933     168     297

Cuts at:   5317    5394 
   Size:         77

  Fragments arranged by size: 

                1481    1407     933     436     390     297     205     168
                  77
 
Bpu10I CC'TnA_GC

Cuts at:      0     905    1007    1534    1712    1788    1895    3750    4047 
   Size:        905     102     527     178      76     107    1855     297

Cuts at:   4047    4198    4318    4420    4790    5234    5394 
   Size:        151     120     102     370     444     160

  Fragments arranged by size: 

                1855     905     527     444     370     297     178     160
                 151     120     107     102     102      76
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1235    2068    2345    5394 
   Size:       1235     833     277    3049

  Fragments arranged by size: 

                3049    1235     833     277
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1305    3395    5394 
   Size:       1305    2090    1999

  Fragments arranged by size: 

                2090    1999    1305
 
BsaAI yAC'GTr

Cuts at:      0     838     949    4723    5394 
   Size:        838     111    3774     671

  Fragments arranged by size: 

                3774     838     671     111
 
BsaBI GATnn'nnATC

Cuts at:      0     980    5394 
   Size:        980    4414
 
BsaHI Gr'CG_yC

Cuts at:      0     282     999    1149    2430    5394 
   Size:        282     717     150    1281    2964

  Fragments arranged by size: 

                2964    1281     717     282     150
 
BsaWI w'CCGG_w

Cuts at:      0    4688    5394 
   Size:       4688     706
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     409     439    5394 
   Size:        409      30    4955

  Fragments arranged by size: 

                4955     409      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2266    2293    3802    4849    5394 
   Size:       2266      27    1509    1047     545

  Fragments arranged by size: 

                2266    1509    1047     545      27
 
BseYI C'CCAG_C

Cuts at:      0     218    1144    2105    2266    2846    3126    3669    3711 
   Size:        218     926     961     161     580     280     543      42

Cuts at:   3711    4635    4784    4841    5394 
   Size:        924     149      57     553

  Fragments arranged by size: 

                 961     926     924     580     553     543     280     218
                 161     149      57      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1271    1945    2517    2982    3096    3622    3742    3749 
   Size:       1271     674     572     465     114     526     120       7

Cuts at:   3749    4964    5162    5392    5394 
   Size:       1215     198     230       2

  Fragments arranged by size: 

                1271    1215     674     572     526     465     230     198
                 120     114       7       2
 
BsiHKAI G_wGCw'C

Cuts at:      0     879     920    1163    1444    1736    2711    3392    3824 
   Size:        879      41     243     281     292     975     681     432

Cuts at:   3824    4092    4628    4963    5394 
   Size:        268     536     335     431

  Fragments arranged by size: 

                 975     879     681     536     432     431     335     292
                 281     268     243      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     879     894     920    1055    1163    1339    1376    1429 
   Size:        879      15      26     135     108     176      37      53

Cuts at:   1429    1444    1612    1736    1886    1976    2106    2267    2340 
   Size:         15     168     124     150      90     130     161      73

Cuts at:   2340    2451    2514    2711    2907    2915    3060    3133    3151 
   Size:        111      63     197     196       8     145      73      18

Cuts at:   3151    3196    3220    3240    3392    3531    3741    3824    4092 
   Size:         45      24      20     152     139     210      83     268

Cuts at:   4092    4167    4236    4368    4398    4473    4628    4851    4956 
   Size:         75      69     132      30      75     155     223     105

Cuts at:   4956    4963    4980    5073    5085    5295    5394 
   Size:          7      17      93      12     210      99

  Fragments arranged by size: 

                 879     268     223     210     210     197     196     176
                 168     161     155     152     150     145     139     135
                 132     130     124     111     108     105      99      93
                  90      83      75      75      73      73      69      63
                  53      45      37      30      26      24      20      18
                  17      15      15      12       8       7
 
BspEI T'CCGG_A

Cuts at:      0    4688    5394 
   Size:       4688     706
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167     251     998    1208    1553    1650    1719    1874 
   Size:        167      84     747     210     345      97      69     155

Cuts at:   1874    3131    3325    4930    4957    5358    5394 
   Size:       1257     194    1605      27     401      36

  Fragments arranged by size: 

                1605    1257     747     401     345     210     194     167
                 155      97      84      69      36      27
 
BsrBI CCG'CTC

Cuts at:      0    1223    4950    5233    5289    5394 
   Size:       1223    3727     283      56     105

  Fragments arranged by size: 

                3727    1223     283     105      56
 
BsrDI GCAATG_nn'

Cuts at:      0    1107    1357    1906    2551    2643    2723    3022    4332 
   Size:       1107     250     549     645      92      80     299    1310

Cuts at:   4332    5394 
   Size:       1062

  Fragments arranged by size: 

                1310    1107    1062     645     549     299     250      92
                  80
 
BsrFI r'CCGG_y

Cuts at:      0    1400    2621    2771    2942    3352    4276    5394 
   Size:       1400    1221     150     171     410     924    1118

  Fragments arranged by size: 

                1400    1221    1118     924     410     171     150
 
BssSI C'ACGA_G

Cuts at:      0    2503    3440    5394 
   Size:       2503     937    1954

  Fragments arranged by size: 

                2503    1954     937
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1343    1971    3021    3651    4252    4758    5068    5394 
   Size:       1343     628    1050     630     601     506     310     326

  Fragments arranged by size: 

                1343    1050     630     628     601     506     326     310
            
 
BstEII G'GTnAC_C

Cuts at:      0     396    1755    5394 
   Size:        396    1359    3639

  Fragments arranged by size: 

                3639    1359     396
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     408     483     587    5394 
   Size:        408      75     104    4807

  Fragments arranged by size: 

                4807     408     104      75
 
BstYI r'GATC_y

Cuts at:      0     794    1206    1465    1590    2985    4652    5375    5394 
   Size:        794     412     259     125    1395    1667     723      19

  Fragments arranged by size: 

                1667    1395     794     723     412     259     125      19
            
 
BstZ17I GTA'TAC

Cuts at:      0    1086    5394 
   Size:       1086    4308
 
BtgI C'CryG_G

Cuts at:      0     149     580     784    1047    1241    2736    2746    3078 
   Size:        149     431     204     263     194    1495      10     332

Cuts at:   3078    3267    3531    5394 
   Size:        189     264    1863

  Fragments arranged by size: 

                1863    1495     431     332     264     263     204     194
                 189     149      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     301    1419    2904    5394 
   Size:        301    1118    1485    2490

  Fragments arranged by size: 

                2490    1485    1118     301
 
BtsI GCAGTG_nn'

Cuts at:      0     973    1623    2862    2970    3060    3151    3189    3196 
   Size:        973     650    1239     108      90      91      38       7

Cuts at:   3196    3342    3343    3421    3480    3761    3928    4889    5073 
   Size:        146       1      78      59     281     167     961     184

Cuts at:   5073    5394 
   Size:        321

  Fragments arranged by size: 

                1239     973     961     650     321     281     184     167
                 146     108      91      90      78      59      38       7
                   1
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     930     965    1089    1124    1684    1719    5394 
   Size:        930      35     124      35     560      35    3675

  Fragments arranged by size: 

                3675     930     560     124      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3055    3892    4252    5394 
   Size:       3055     837     360    1142

  Fragments arranged by size: 

                3055    1142     837     360
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3101    5394 
   Size:       3101    2293
 
EaeI y'GGCC_r

Cuts at:      0     504    1014    1079    1305    1521    2598    2623    2940 
   Size:        504     510      65     226     216    1077      25     317

Cuts at:   2940    3264    4151    4607    5394 
   Size:        324     887     456     787

  Fragments arranged by size: 

                1077     887     787     510     504     456     324     317
                 226     216      65      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3381    5394 
   Size:       3381    2013
 
EcoICRI GAG'CTC

Cuts at:      0    1161    3390    4626    4961    5394 
   Size:       1161    2229    1236     335     433

  Fragments arranged by size: 

                2229    1236    1161     433     335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     659    1843    2098    2233    2438    2810    3919 
   Size:        436     223    1184     255     135     205     372    1109

Cuts at:   3919    4625    4852    5020    5317    5394 
   Size:        706     227     168     297      77

  Fragments arranged by size: 

                1184    1109     706     436     372     297     255     227
                 223     205     168     135      77
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4486    4570    4918    5394 
   Size:       4486      84     348     476

  Fragments arranged by size: 

                4486     476     348      84
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99    1197    1530    1890    1936    2742    2794    2922 
   Size:         99    1098     333     360      46     806      52     128

Cuts at:   2922    3216    3217    3310    4314    5142    5217    5394 
   Size:        294       1      93    1004     828      75     177

  Fragments arranged by size: 

                1098    1004     828     806     360     333     294     177
                 128      99      93      75      52      46       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     279    3090    4149    5394 
   Size:        279    2811    1059    1245

  Fragments arranged by size: 

                2811    1245    1059     279
 
FspI TGC'GCA

Cuts at:      0    2295    5341    5394 
   Size:       2295    3046      53

  Fragments arranged by size: 

                3046    2295      53
 
HaeII r_GCGC'y

Cuts at:      0     285    1152    1752    2220    2433    2675    3630    5394 
   Size:        285     867     600     468     213     242     955    1764

  Fragments arranged by size: 

                1764     955     867     600     468     285     242     213
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     914     946    3421    3453    4104    4136    5394 
   Size:        914      32    2475      32     651      32    1258

  Fragments arranged by size: 

                2475    1258     914     651      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    2897    5394 
   Size:       2897    2497
 
KasI G'GCGC_C

Cuts at:      0     281    1148    2429    5394 
   Size:        281     867    1281    2965

  Fragments arranged by size: 

                2965    1281     867     281
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1892    4967    5297    5306    5394 
   Size:       1892    3075     330       9      88

  Fragments arranged by size: 

                3075    1892     330      88       9
 
MscI TGG'CCA

Cuts at:      0    1523    2600    3266    4153    4609    5394 
   Size:       1523    1077     666     887     456     785

  Fragments arranged by size: 

                1523    1077     887     785     666     456
 
MslI CAynn'nnrTG

Cuts at:      0     481     590    1022    2349    2648    2834    3347    3425 
   Size:        481     109     432    1327     299     186     513      78

Cuts at:   3425    3459    4600    4615    5394 
   Size:         34    1141      15     779

  Fragments arranged by size: 

                1327    1141     779     513     481     432     299     186
                 109      78      34      15
 
MspA1I CmG'CkG

Cuts at:      0     354     408    1064    1144    1511    2435    2588    3110 
   Size:        354      54     656      80     367     924     153     522

Cuts at:   3110    3233    3488    3639    4161    4322    4336    4392    4467 
   Size:        123     255     151     522     161      14      56      75

Cuts at:   4467    4645    4845    5100    5250    5394 
   Size:        178     200     255     150     144

  Fragments arranged by size: 

                 924     656     522     522     367     354     255     255
                 200     178     161     153     151     150     144     123
                  80      75      56      54      14
 
NaeI GCC'GGC

Cuts at:      0    1402    2623    2773    3354    4278    5394 
   Size:       1402    1221     150     581     924    1116

  Fragments arranged by size: 

                1402    1221    1116     924     581     150
 
NarI GG'CG_CC

Cuts at:      0     282    1149    2430    5394 
   Size:        282     867    1281    2964

  Fragments arranged by size: 

                2964    1281     867     282
 
NcoI C'CATG_G

Cuts at:      0    1047    1241    3078    3267    5394 
   Size:       1047     194    1837     189    2127

  Fragments arranged by size: 

                2127    1837    1047     194     189
 
NdeI CA'TA_TG

Cuts at:      0    2757    5394 
   Size:       2757    2637
 
NgoMIV G'CCGG_C

Cuts at:      0    1400    2621    2771    3352    4276    5394 
   Size:       1400    1221     150     581     924    1118

  Fragments arranged by size: 

                1400    1221    1118     924     581     150
 
NsiI A_TGCA'T

Cuts at:      0    3576    5394 
   Size:       3576    1818
 
NspI r_CATG'y

Cuts at:      0     520    1330    2014    2647    3119    3578    3702    4006 
   Size:        520     810     684     633     472     459     124     304

Cuts at:   4006    5394 
   Size:       1388

  Fragments arranged by size: 

                1388     810     684     633     520     472     459     304
                 124
 
PasI CC'CwG_GG

Cuts at:      0      44     886    2797    2908    3406    3496    4863    5073 
   Size:         44     842    1911     111     498      90    1367     210

Cuts at:   5073    5394 
   Size:        321

  Fragments arranged by size: 

                1911    1367     842     498     321     210     111      90
                  44
 
PciI A'CATG_T

Cuts at:      0    1326    5394 
   Size:       1326    4068
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1638    3184    3248    4662    5394 
   Size:       1638    1546      64    1414     732

  Fragments arranged by size: 

                1638    1546    1414     732      64
 
PfoI T'CCnGG_A

Cuts at:      0    1688    1777    4997    5394 
   Size:       1688      89    3220     397

  Fragments arranged by size: 

                3220    1688     397      89
 
PmlI CAC'GTG

Cuts at:      0     949    5394 
   Size:        949    4445
 
PpuMI rG'GwC_Cy

Cuts at:      0    1197    1890    2742    2922    5217    5394 
   Size:       1197     693     852     180    2295     177

  Fragments arranged by size: 

                2295    1197     852     693     180     177
 
PshAI GACnn'nnGTC

Cuts at:      0    1259    5394 
   Size:       1259    4135
 
PspOMI G'GGCC_C

Cuts at:      0    3216    4364    4469    5394 
   Size:       3216    1148     105     925

  Fragments arranged by size: 

                3216    1148     925     105
 
PspXI vC'TCGA_Gb

Cuts at:      0    3835    5394 
   Size:       3835    1559
 
PstI C_TGCA'G

Cuts at:      0     248    3487    4023    4391    4763    4968    5249    5394 
   Size:        248    3239     536     368     372     205     281     145

  Fragments arranged by size: 

                3239     536     372     368     281     248     205     145
            
 
PvuII CAG'CTG

Cuts at:      0     408    1064    1144    3488    4322    4645    5100    5394 
   Size:        408     656      80    2344     834     323     455     294

  Fragments arranged by size: 

                2344     834     656     455     408     323     294      80
            
 
SacI G_AGCT'C

Cuts at:      0    1163    3392    4628    4963    5394 
   Size:       1163    2229    1236     335     431

  Fragments arranged by size: 

                2229    1236    1163     431     335
 
SanDI GG'GwC_CC

Cuts at:      0    2742    2922    5394 
   Size:       2742     180    2472

  Fragments arranged by size: 

                2742    2472     180
 
SbfI CC_TGCA'GG

Cuts at:      0     248    5394 
   Size:        248    5146
 
ScaI AGT'ACT

Cuts at:      0     808    5394 
   Size:        808    4586
 
SexAI A'CCwGG_T

Cuts at:      0    4919    5394 
   Size:       4919     475
 
SfcI C'TryA_G

Cuts at:      0     244     693    1029    1804    3483    4019    4340    4387 
   Size:        244     449     336     775    1679     536     321      47

Cuts at:   4387    4727    4759    4964    5045    5245    5254    5394 
   Size:        340      32     205      81     200       9     140

  Fragments arranged by size: 

                1679     775     536     449     340     336     321     244
                 205     200     140      81      47      32       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     212    1529    5394 
   Size:        212    1317    3865

  Fragments arranged by size: 

                3865    1317     212
 
SfoI GGC'GCC

Cuts at:      0     283    1150    2431    5394 
   Size:        283     867    1281    2963

  Fragments arranged by size: 

                2963    1281     867     283
 
SmaI CCC'GGG

Cuts at:      0    1764    5394 
   Size:       1764    3630
 
SmlI C'TyrA_G

Cuts at:      0      12    1250    2047    2360    3835    5394 
   Size:         12    1238     797     313    1475    1559

  Fragments arranged by size: 

                1559    1475    1238     797     313      12
 
SphI G_CATG'C

Cuts at:      0    2647    3578    4006    5394 
   Size:       2647     931     428    1388

  Fragments arranged by size: 

                2647    1388     931     428
 
SspI AAT'ATT

Cuts at:      0    2842    5394 
   Size:       2842    2552
 
StuI AGG'CCT

Cuts at:      0    2290    5394 
   Size:       2290    3104
 
StyI C'CwwG_G

Cuts at:      0    1047    1193    1241    1976    1992    3078    3267    5163 
   Size:       1047     146      48     735      16    1086     189    1896

Cuts at:   5163    5394 
   Size:        231

  Fragments arranged by size: 

                1896    1086    1047     735     231     189     146      48
                  16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1072    1567    2677    3308    3608    3661    3970    5107 
   Size:       1072     495    1110     631     300      53     309    1137

Cuts at:   5107    5394 
   Size:        287

  Fragments arranged by size: 

                1137    1110    1072     631     495     309     300     287
                  53
 
TatI w'GTAC_w

Cuts at:      0     806    4818    5394 
   Size:        806    4012     576

  Fragments arranged by size: 

                4012     806     576
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1582    1614    3030    3062    4926    4958    5394 
   Size:       1582      32    1416      32    1864      32     436

  Fragments arranged by size: 

                1864    1582    1416     436      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1639    1957    3067    3529    3889    4165    4509    5394 
   Size:       1639     318    1110     462     360     276     344     885

  Fragments arranged by size: 

                1639    1110     885     462     360     344     318     276
            
 
XhoI C'TCGA_G

Cuts at:      0    3835    5394 
   Size:       3835    1559
 
XmaI C'CCGG_G

Cuts at:      0    1762    5394 
   Size:       1762    3632
 
XmnI GAAnn'nnTTC

Cuts at:      0     262    4561    5394 
   Size:        262    4299     833

  Fragments arranged by size: 

                4299     833     262
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI
     EaeI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SacI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI     BcgI
     BmtI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrGI   BssHII
    BstBI   Bsu36I     ClaI     DraI     EagI     EarI    EcoRI    EcoRV
     FseI    FspAI   HincII     HpaI     KpnI     MfeI     MluI     NheI
     NotI     NruI     PacI     PmeI     PpiI     PsiI     PsrI     PvuI
    RsrII    SacII     SalI     SapI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SwaI  Tth111I     XbaI     ZraI
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