Restriction map for pF1KE3826
##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AclI        AgeI        AscI        AseI        AsnI        
BciVI       Bsp1407I    BspDI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
DraI        EcoO65I     EcoRV       EcoT22I     FseI        
HindIII     HpaI        MfeI        MunI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshBI       Psp1406I    PvuI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AatII        6079  GACGTC               GACGT^C
Acc65I       2189  GGTACC               G^GTACC
AcsI         6955  GAATTC               [GA]^AATT[CT]
AflII        7600  CTTAAG               C^TTAAG
AocI         7315  CCTGAGG              CC^T[ACGT]AGG
ApoI         6955  GAATTC               [GA]^AATT[CT]
Asp718I      2189  GGTACC               G^GTACC
AvrII        7977  CCTAGG               C^CTAGG
BamHI        6731  GGATCC               G^GATCC
BfaI         7978  CTAG                 C^TAG
BfrI         7600  CTTAAG               C^TTAAG
BlnI         7977  CCTAGG               C^CTAGG
BsiWI        1154  CGTACG               C^GTACG
BssHII       2319  GCGCGC               G^CGCGC
Bsu36I       7315  CCTGAGG              CC^T[ACGT]AGG
CpoI         1999  CGGACCG              CG^G[AT]CCG
Eco81I       7315  CCTGAGG              CC^T[ACGT]AGG
EcoRI        6955  GAATTC               G^AATTC
KpnI         2189  GGTACC               GGTAC^C
KspI          119  CCGCGG               CCGC^GG
MaeI         7978  CTAG                 C^TAG
MluI         2459  ACGCGT               A^CGCGT
NotI         3152  GCGGCCGC             GC^GGCCGC
RsrII        1999  CGGACCG              CG^G[AT]CCG
SacII         119  CCGCGG               CCGC^GG
SbfI         6099  CCTGCAGG             CCTGCA^GG
SexAI        1433  ACCTGGT              A^CC[AT]GGT
SfiI         1015  GGCCGCACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     6099  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII       1997  TCCGGA               T^CCGGA
             4577  TCCGGA              
AhdI         4183  GACGTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5335  GACAAGAAGTC         
AspEI        4183  GACGTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5335  GACAAGAAGTC         
AviII        1865  TGCGCA               TGC^GCA
             5057  TGCGCA              
BbeI         3424  GGCGCC               GGCGC^C
             3881  GGCGCC              
BbvCI        6305  CCTCAGC              CCTCAGC(-5/-2)
              949  GCTGAGG             
BcgI         4487  CGATTGTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1079  GCAATGACATCG        
BseAI        1997  TCCGGA               T^CCGGA
             4577  TCCGGA              
BspEI        1997  TCCGGA               T^CCGGA
             4577  TCCGGA              
BspLU11I     1652  ACATGT               A^CATGT
             7691  ACATGT              
EagI          122  CGGCCG               C^GGCCG
             3153  CGGCCG              
Eam1105I     4183  GACGTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5335  GACAAGAAGTC         
EclXI         122  CGGCCG               C^GGCCG
             3153  CGGCCG              
Eco52I        122  CGGCCG               C^GGCCG
             3153  CGGCCG              
FspI         1865  TGCGCA               TGC^GCA
             5057  TGCGCA              
KasI         3424  GGCGCC               G^GCGCC
             3881  GGCGCC              
MroI         1997  TCCGGA               T^CCGGA
             4577  TCCGGA              
MseI         3920  TTAA                 T^TAA
             7601  TTAA                
NarI         3424  GGCGCC               GG^CGCC
             3881  GGCGCC              
PaeR7I       4525  CTCGAG               C^TCGAG
             7189  CTCGAG              
PshAI        1848  GACCTTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             6984  GACCAGTGTC          
SalI         5464  GTCGAC               G^TCGAC
             7195  GTCGAC              
SfoI         3424  GGCGCC               GGC^GCC
             3881  GGCGCC              
Tru9I        3920  TTAA                 T^TAA
             7601  TTAA                
XhoI         4525  CTCGAG               C^TCGAG
             7189  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  158 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaI        ApaLI       Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbsI        BbvI        BclI        
BcnI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DrdI        
DsaI        EaeI        EarI        Eco47III    EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SphI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmnI        
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