Restriction map for pF1KE3826
 (Linear) (Six-base) MAPSORT of: KIEE3826.seq  Check: 9188  from: 1  to: 8013



KIEE3826.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     774    1264    3755    3872    3973    4040    5285    6215 
   Size:        774     490    2491     117     101      67    1245     930

Cuts at:   6215    7279    8013 
   Size:       1064     734

  Fragments arranged by size: 

                2491    1245    1064     930     774     734     490     117
                 101      67
 
AatII G_ACGT'C

Cuts at:      0    6083    8013 
   Size:       6083    1930
 
AccI GT'mk_AC

Cuts at:      0    4142    4510    5465    7139    7196    8013 
   Size:       4142     368     955    1674      57     817

  Fragments arranged by size: 

                4142    1674     955     817     368      57
 
Acc65I G'GTAC_C

Cuts at:      0    2189    8013 
   Size:       2189    5824
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1585    2218    2616    2790    2908    2926    3228    3610 
   Size:       1585     633     398     174     118      18     302     382

Cuts at:   3610    4422    4837    6232    8013 
   Size:        812     415    1395    1781

  Fragments arranged by size: 

                1781    1585    1395     812     633     415     398     382
                 302     174     118      18
 
AfeI AGC'GCT

Cuts at:      0    4909    5350    6772    8013 
   Size:       4909     441    1422    1241

  Fragments arranged by size: 

                4909    1422    1241     441
 
AflII C'TTAA_G

Cuts at:      0    7600    8013 
   Size:       7600     413
 
AflIII A'CryG_T

Cuts at:      0     757    1184    1652    2459    5568    6359    7691    8013 
   Size:        757     427     468     807    3109     791    1332     322

  Fragments arranged by size: 

                3109    1332     807     791     757     468     427     322
            
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4188    5340    8013 
   Size:       4188    1152    2673

  Fragments arranged by size: 

                4188    2673    1152
 
AleI CACnn'nnGTG

Cuts at:      0    1204    2779    4222    4273    5596    6694    8013 
   Size:       1204    1575    1443      51    1323    1098    1319

  Fragments arranged by size: 

                1575    1443    1323    1319    1204    1098      51
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1222    1981    2752    3181    3215    4183    4486    5008 
   Size:       1222     759     771     429      34     968     303     522

Cuts at:   5008    5071    5083    5614    8013 
   Size:         63      12     531    2399

  Fragments arranged by size: 

                2399    1222     968     771     759     531     522     429
                 303      63      34      12
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1870    1902    4450    4482    5080    5112    6346    6378 
   Size:       1870      32    2548      32     598      32    1234      32

Cuts at:   6378    7048    7080    8013 
   Size:        670      32     933

  Fragments arranged by size: 

                2548    1870    1234     933     670     598      32      32
                  32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     276     372     579    1425    1521    2262    2376    3040 
   Size:        276      96     207     846      96     741     114     664

Cuts at:   3040    3291    3693    3732    3897    4503    5016    5637    5730 
   Size:        251     402      39     165     606     513     621      93

Cuts at:   5730    6072    6207    6438    8013 
   Size:        342     135     231    1575

  Fragments arranged by size: 

                1575     846     741     664     621     606     513     402
                 342     276     251     231     207     165     135     114
                  96      96      93      39
 
ApaI G_GGCC'C

Cuts at:      0    2693    5388    5536    5851    6839    7756    8013 
   Size:       2693    2695     148     315     988     917     257

  Fragments arranged by size: 

                2695    2693     988     917     315     257     148
 
ApaLI G'TGCA_C

Cuts at:      0    2350    3859    4158    8013 
   Size:       2350    1509     299    3855

  Fragments arranged by size: 

                3855    2350    1509     299
 
ApoI r'AATT_y

Cuts at:      0    6955    8013 
   Size:       6955    1058
 
AvaI C'yCGr_G

Cuts at:      0    2465    3213    4346    4525    7189    7475    8013 
   Size:       2465     748    1133     179    2664     286     538

  Fragments arranged by size: 

                2664    2465    1133     748     538     286     179
 
AvrII C'CTAG_G

Cuts at:      0    7977    8013 
   Size:       7977      36
 
BamHI G'GATC_C

Cuts at:      0    6731    8013 
   Size:       6731    1282
 
BanI G'GyrC_C

Cuts at:      0    1169    1252    2189    3217    3424    3881    6856    6913 
   Size:       1169      83     937    1028     207     457    2975      57

Cuts at:   6913    7311    8013 
   Size:        398     702

  Fragments arranged by size: 

                2975    1169    1028     937     702     457     398     207
                  83      57
 
BanII G_rGCy'C

Cuts at:      0      61     119     602    1883    1952    2693    3773    4561 
   Size:         61      58     483    1281      69     741    1080     788

Cuts at:   4561    5388    5536    5780    5851    6136    6839    7307    7756 
   Size:        827     148     244      71     285     703     468     449

Cuts at:   7756    7937    8013 
   Size:        181      76

  Fragments arranged by size: 

                1281    1080     827     788     741     703     483     468
                 449     285     244     181     148      76      71      69
                  61      58
 
BbeI G_GCGC'C

Cuts at:      0    3428    3885    8013 
   Size:       3428     457    4128

  Fragments arranged by size: 

                4128    3428     457
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1436    2796    2970    3124    5032    6338    8013 
   Size:       1436    1360     174     154    1908    1306    1675

  Fragments arranged by size: 

                1908    1675    1436    1360    1306     174     154
 
BbvCI CC'TCA_GC

Cuts at:      0     950    6306    8013 
   Size:        950    5356    1707

  Fragments arranged by size: 

                5356    1707     950
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1068    1102    4476    4510    8013 
   Size:       1068      34    3374      34    3503

  Fragments arranged by size: 

                3503    3374    1068      34      34
 
BclI T'GATC_A

Cuts at:      0    3326    3959    7778    8013 
   Size:       3326     633    3819     235

  Fragments arranged by size: 

                3819    3326     633     235
 
BglI GCCn_nnn'nGGC

Cuts at:      0     823    1022    1239    2140    4594    7123    8013 
   Size:        823     199     217     901    2454    2529     890

  Fragments arranged by size: 

                2529    2454     901     890     823     217     199
 
BglII A'GATC_T

Cuts at:      0    2249    4289    5243    8013 
   Size:       2249    2040     954    2770

  Fragments arranged by size: 

                2770    2249    2040     954
 
BlpI GC'TnA_GC

Cuts at:      0     601    2176    2785    2843    4405    4654    4835    6038 
   Size:        601    1575     609      58    1562     249     181    1203

Cuts at:   6038    6265    8013 
   Size:        227    1748

  Fragments arranged by size: 

                1748    1575    1562    1203     609     601     249     227
                 181      58
 
Bme1580I G_kGCm'C

Cuts at:      0     181     896    1011    1145    1211    1255    1304    2354 
   Size:        181     715     115     134      66      44      49    1050

Cuts at:   2354    2549    2693    2765    2814    3222    3863    4162    5388 
   Size:        195     144      72      49     408     641     299    1226

Cuts at:   5388    5536    5555    5593    5851    6422    6839    6861    7756 
   Size:        148      19      38     258     571     417      22     895

Cuts at:   7756    8013 
   Size:        257

  Fragments arranged by size: 

                1226    1050     895     715     641     571     417     408
                 299     258     257     195     181     148     144     134
                 115      72      66      49      49      44      38      22
                  19
 
BmgBI CAC'GTC

Cuts at:      0     717     758    3309    4185    6362    8013 
   Size:        717      41    2551     876    2177    1651

  Fragments arranged by size: 

                2551    2177    1651     876     717      41
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     956    5166    6409    8013 
   Size:        956    4210    1243    1604

  Fragments arranged by size: 

                4210    1604    1243     956
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    6270    8013 
   Size:       6270    1743
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     712    1633    3154    3796    4198    4683    4825 
   Size:        448     264     921    1521     642     402     485     142

Cuts at:   4825    4906    5068    6165    7648    7702    7858    8013 
   Size:         81     162    1097    1483      54     156     155

  Fragments arranged by size: 

                1521    1483    1097     921     642     485     448     402
                 264     162     156     155     142      81      54
 
Bpu10I CC'TnA_GC

Cuts at:      0     950    2047    4871    6306    8013 
   Size:        950    1097    2824    1435    1707

  Fragments arranged by size: 

                2824    1707    1435    1097     950
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    5005    5959    6283    7156    7921    8013 
   Size:       5005     954     324     873     765      92

  Fragments arranged by size: 

                5005     954     873     765     324      92
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1313    1840    2890    3216    3778    5389    5818    8013 
   Size:       1313     527    1050     326     562    1611     429    2195

  Fragments arranged by size: 

                2195    1611    1313    1050     562     527     429     326
            
 
BsaAI yAC'GTr

Cuts at:      0    1187    2084    2349    2355    4263    4401    6139    6969 
   Size:       1187     897     265       6    1908     138    1738     830

Cuts at:   6969    7767    8013 
   Size:        798     246

  Fragments arranged by size: 

                1908    1738    1187     897     830     798     265     246
                 138       6
 
BsaBI GATnn'nnATC

Cuts at:      0    3667    6736    7381    8013 
   Size:       3667    3069     645     632

  Fragments arranged by size: 

                3667    3069     645     632
 
BsaHI Gr'CG_yC

Cuts at:      0    1340    3425    3882    6080    8013 
   Size:       1340    2085     457    2198    1933

  Fragments arranged by size: 

                2198    2085    1933    1340     457
 
BsaWI w'CCGG_w

Cuts at:      0    1997    4577    5090    8013 
   Size:       1997    2580     513    2923

  Fragments arranged by size: 

                2923    2580    1997     513
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25      55     685     704     715     734    1330    1360 
   Size:         25      30     630      19      11      19     596      30

Cuts at:   1360    1606    1636    1870    1900    2236    2266    3088    3118 
   Size:        246      30     234      30     336      30     822      30

Cuts at:   3118    4543    4573    4966    4996    5386    5416    5971    6001 
   Size:       1425      30     393      30     390      30     555      30

Cuts at:   6001    6508    6538    6574    6604    6670    6700    7048    7078 
   Size:        507      30      36      30      66      30     348      30

Cuts at:   7078    7132    7162    7201    7231    8013 
   Size:         54      30      39      30     782

  Fragments arranged by size: 

                1425     822     782     630     596     555     507     393
                 390     348     336     246     234      66      54      39
                  36      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      25      19      19      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     709     899     967    1237    1240    1630    2575    2884 
   Size:        709     190      68     270       3     390     945     309

Cuts at:   2884    3424    3475    4513    4822    4987    5502    6250    6253 
   Size:        540      51    1038     309     165     515     748       3

Cuts at:   6253    6337    6340    7069    7606    8013 
   Size:         84       3     729     537     407

  Fragments arranged by size: 

                1038     945     748     729     709     540     537     515
                 407     390     309     309     270     190     165      84
                  68      51       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     889    1036    1248    2255    2262    2664    2815    2827 
   Size:        889     147     212    1007       7     402     151      12

Cuts at:   2827    4685    5387    5529    5536    5772    7470    7922    8013 
   Size:       1858     702     142       7     236    1698     452      91

  Fragments arranged by size: 

                1858    1698    1007     889     702     452     402     236
                 212     151     147     142      91      12       7       7
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358    1161    1780    2329    2704    3457    3721    3728 
   Size:        358     803     619     549     375     753     264       7

Cuts at:   3728    3733    3892    3910    4516    4694    4846    5350    5593 
   Size:          5     159      18     606     178     152     504     243

Cuts at:   5593    6517    8013 
   Size:        924    1496

  Fragments arranged by size: 

                1496     924     803     753     619     606     549     504
                 375     358     264     243     178     159     152      18
                   7       5
 
BsiEI CG_ry'CG

Cuts at:      0     125    3156    5373    7200    8013 
   Size:        125    3031    2217    1827     813

  Fragments arranged by size: 

                3031    2217    1827     813     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     602    1201    1718    1952    2177    2354    3176    3863 
   Size:        602     599     517     234     225     177     822     687

Cuts at:   3863    4162    5603    7688    7937    8013 
   Size:        299    1441    2085     249      76

  Fragments arranged by size: 

                2085    1441     822     687     602     599     517     299
                 249     234     225     177      76
 
BsiWI C'GTAC_G

Cuts at:      0    1154    8013 
   Size:       1154    6859
 
BsmI GAATG_Cn'

Cuts at:      0    1038    1559    1683    6227    8013 
   Size:       1038     521     124    4544    1786

  Fragments arranged by size: 

                4544    1786    1038     521     124
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      45     707    2407    5710    7051    8013 
   Size:         45     662    1700    3303    1341     962

  Fragments arranged by size: 

                3303    1700    1341     962     662      45
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     119     181     602     896    1011    1145    1201 
   Size:         61      58      62     421     294     115     134      56

Cuts at:   1201    1211    1255    1304    1718    1883    1952    2177    2354 
   Size:         10      44      49     414     165      69     225     177

Cuts at:   2354    2549    2693    2765    2814    3176    3222    3773    3863 
   Size:        195     144      72      49     362      46     551      90

Cuts at:   3863    4162    4561    5388    5536    5555    5593    5603    5780 
   Size:        299     399     827     148      19      38      10     177

Cuts at:   5780    5851    6136    6422    6839    6861    7307    7688    7756 
   Size:         71     285     286     417      22     446     381      68

Cuts at:   7756    7937    8013 
   Size:        181      76

  Fragments arranged by size: 

                 827     551     446     421     417     414     399     381
                 362     299     294     286     285     225     195     181
                 177     177     165     148     144     134     115      90
                  76      72      71      69      68      62      61      58
                  56      49      49      46      44      38      22      19
                  10      10
 
BspEI T'CCGG_A

Cuts at:      0    1997    4577    8013 
   Size:       1997    2580    3436

  Fragments arranged by size: 

                3436    2580    1997
 
BspHI T'CATG_A

Cuts at:      0    2109    3962    7008    7394    8013 
   Size:       2109    1853    3046     386     619

  Fragments arranged by size: 

                3046    2109    1853     619     386
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     774    1264    1397    2324    2975    3270    3632    3755 
   Size:        774     490     133     927     651     295     362     123

Cuts at:   3755    3872    3973    4040    4118    5285    5669    6107    6215 
   Size:        117     101      67      78    1167     384     438     108

Cuts at:   6215    7279    7574    8013 
   Size:       1064     295     439

  Fragments arranged by size: 

                1167    1064     927     774     651     490     439     438
                 384     362     295     295     133     123     117     108
                 101      78      67
 
BsrBI CCG'CTC

Cuts at:      0    1230    4812    7100    7356    8013 
   Size:       1230    3582    2288     256     657

  Fragments arranged by size: 

                3582    2288    1230     657     256
 
BsrDI GCAATG_nn'

Cuts at:      0    1086    1718    6653    6718    7098    7842    8013 
   Size:       1086     632    4935      65     380     744     171

  Fragments arranged by size: 

                4935    1086     744     632     380     171      65
 
BsrFI r'CCGG_y

Cuts at:      0     613    2149    2385    3344    5177    6017    6216    6458 
   Size:        613    1536     236     959    1833     840     199     242

Cuts at:   6458    7199    8013 
   Size:        741     814

  Fragments arranged by size: 

                1833    1536     959     840     814     741     613     242
                 236     199
 
BssHII G'CGCG_C

Cuts at:      0    2319    8013 
   Size:       2319    5694
 
BssSI C'ACGA_G

Cuts at:      0    2038    5554    5884    8013 
   Size:       2038    3516     330    2129

  Fragments arranged by size: 

                3516    2129    2038     330
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     276     579    3585    3858    4503    5013    5016    6207 
   Size:        276     303    3006     273     645     510       3    1191

Cuts at:   6207    8013 
   Size:       1806

  Fragments arranged by size: 

                3006    1806    1191     645     510     303     276     273
                   3
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1365    1890    2499    3663    4629    5205    6945    6993 
   Size:       1365     525     609    1164     966     576    1740      48

Cuts at:   6993    7233    7902    8013 
   Size:        240     669     111

  Fragments arranged by size: 

                1740    1365    1164     966     669     609     576     525
                 240     111      48
 
BstYI r'GATC_y

Cuts at:      0     984    1313    2249    2414    2909    3632    3668    4289 
   Size:        984     329     936     165     495     723      36     621

Cuts at:   4289    5243    5300    5958    6731    8013 
   Size:        954      57     658     773    1282

  Fragments arranged by size: 

                1282     984     954     936     773     723     658     621
                 495     329     165      57      36
 
Bsu36I CC'TnA_GG

Cuts at:      0    7316    8013 
   Size:       7316     697
 
BtgI C'CryG_G

Cuts at:      0     119     879    1364    2498    2777    3356    3452    4534 
   Size:        119     760     485    1134     279     579      96    1082

Cuts at:   4534    4562    4589    4622    5405    5753    6909    6938    7401 
   Size:         28      27      33     783     348    1156      29     463

Cuts at:   7401    8013 
   Size:        612

  Fragments arranged by size: 

                1156    1134    1082     783     760     612     579     485
                 463     348     279     119      96      33      29      28
                  27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1071    1466    1911    4004    6735    8013 
   Size:       1071     395     445    2093    2731    1278

  Fragments arranged by size: 

                2731    2093    1278    1071     445     395
 
BtsI GCAGTG_nn'

Cuts at:      0     363     802    1784    2659    3056    3402    3501    4254 
   Size:        363     439     982     875     397     346      99     753

Cuts at:   4254    4665    4899    5586    7281    7916    8013 
   Size:        411     234     687    1695     635      97

  Fragments arranged by size: 

                1695     982     875     753     687     635     439     411
                 397     363     346     234      99      97
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    7401    7436    8013 
   Size:       7401      35     577

  Fragments arranged by size: 

                7401     577      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3858    5174    5559    8013 
   Size:       3858    1316     385    2454

  Fragments arranged by size: 

                3858    2454    1316     385
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     529    1489    3994    5886    8013 
   Size:        529     960    2505    1892    2127

  Fragments arranged by size: 

                2505    2127    1892     960     529
 
EaeI y'GGCC_r

Cuts at:      0     122     824    1355    1769    2237    2696    3153    3869 
   Size:        122     702     531     414     468     459     457     716

Cuts at:   3869    4115    4595    5267    6632    7095    8013 
   Size:        246     480     672    1365     463     918

  Fragments arranged by size: 

                1365     918     716     702     672     531     480     468
                 463     459     457     414     246     122
 
EagI C'GGCC_G

Cuts at:      0     122    3153    8013 
   Size:        122    3031    4860

  Fragments arranged by size: 

                4860    3031     122
 
EarI CTCTTCn'nnn_

Cuts at:      0     172     376     490    2060    2110    3238    3760    4885 
   Size:        172     204     114    1570      50    1128     522    1125

Cuts at:   4885    6104    6261    6469    6730    7150    7171    7322    7471 
   Size:       1219     157     208     261     420      21     151     149

Cuts at:   7471    8013 
   Size:        542

  Fragments arranged by size: 

                1570    1219    1128    1125     542     522     420     261
                 208     204     172     157     151     149     114      50
                  21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1648    2932    3109    6772    8013 
   Size:       1648    1284     177    3663    1241

  Fragments arranged by size: 

                3663    1648    1284    1241     177
 
EcoICRI GAG'CTC

Cuts at:      0     600    1950    7935    8013 
   Size:        600    1350    5985      78

  Fragments arranged by size: 

                5985    1350     600      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     712    1585    1633    2218    2616    2790    2908 
   Size:        448     264     873      48     585     398     174     118

Cuts at:   2908    2926    3154    3228    3610    3796    4198    4422    4683 
   Size:         18     228      74     382     186     402     224     261

Cuts at:   4683    4825    4837    4906    5068    6165    6232    7648    7702 
   Size:        142      12      69     162    1097      67    1416      54

Cuts at:   7702    7858    8013 
   Size:        156     155

  Fragments arranged by size: 

                1416    1097     873     585     448     402     398     382
                 264     261     228     224     186     174     162     156
                 155     142     118      74      69      67      54      48
                  18      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     169     694    1546    1771    4828    6103    6442    7327 
   Size:        169     525     852     225    3057    1275     339     885

Cuts at:   7327    8013 
   Size:        686

  Fragments arranged by size: 

                3057    1275     885     852     686     525     339     225
                 169
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129    1569    1597    2689    2690    2718    3729    4956 
   Size:        129    1440      28    1092       1      28    1011    1227

Cuts at:   4956    5112    5533    5658    5847    5848    7320    7753    8013 
   Size:        156     421     125     189       1    1472     433     260

  Fragments arranged by size: 

                1472    1440    1227    1092    1011     433     421     260
                 189     156     129     125      28      28       1       1
            
 
EcoRI G'AATT_C

Cuts at:      0    6955    8013 
   Size:       6955    1058
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1299    7197    8013 
   Size:       1299    5898     816

  Fragments arranged by size: 

                5898    1299     816
 
FspI TGC'GCA

Cuts at:      0    1867    5059    8013 
   Size:       1867    3192    2954

  Fragments arranged by size: 

                3192    2954    1867
 
HaeII r_GCGC'y

Cuts at:      0    3255    3428    3885    4911    5352    6172    6313    6774 
   Size:       3255     173     457    1026     441     820     141     461

Cuts at:   6774    6802    8013 
   Size:         28    1211

  Fragments arranged by size: 

                3255    1211    1026     820     461     457     441     173
                 141      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     540     572    1075    1107    1330    1362 
   Size:         25      32     483      32     503      32     223      32

Cuts at:   1362    1606    1638    1885    1917    2236    2268    4003    4035 
   Size:        244      32     247      32     319      32    1735      32

Cuts at:   4035    4174    4206    5326    5358    5386    5418    5971    6003 
   Size:        139      32    1120      32      28      32     553      32

Cuts at:   6003    6508    6540    6670    6702    7282    7314    8013 
   Size:        505      32     130      32     580      32     699

  Fragments arranged by size: 

                1735    1120     699     580     553     505     503     483
                 319     247     244     223     139     130      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      28      25
 
HincII GTy'rAC

Cuts at:      0    5418    5466    7197    8013 
   Size:       5418      48    1731     816

  Fragments arranged by size: 

                5418    1731     816      48
 
KasI G'GCGC_C

Cuts at:      0    3424    3881    8013 
   Size:       3424     457    4132

  Fragments arranged by size: 

                4132    3424     457
 
KpnI G_GTAC'C

Cuts at:      0    2193    8013 
   Size:       2193    5820
 
MluI A'CGCG_T

Cuts at:      0    2459    8013 
   Size:       2459    5554
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1943    2474    3314    4949    8013 
   Size:       1943     531     840    1635    3064

  Fragments arranged by size: 

                3064    1943    1635     840     531
 
MscI TGG'CCA

Cuts at:      0    1357    2239    3871    4597    5269    8013 
   Size:       1357     882    1632     726     672    2744

  Fragments arranged by size: 

                2744    1632    1357     882     726     672
 
MslI CAynn'nnrTG

Cuts at:      0     338     445     899    1079    1204    2779    2887    3197 
   Size:        338     107     454     180     125    1575     108     310

Cuts at:   3197    3961    4222    4273    4990    5596    6026    6694    6943 
   Size:        764     261      51     717     606     430     668     249

Cuts at:   6943    6949    7393    7519    8013 
   Size:          6     444     126     494

  Fragments arranged by size: 

                1575     764     717     668     606     494     454     444
                 430     338     310     261     249     180     126     125
                 108     107      51       6
 
MspA1I CmG'CkG

Cuts at:      0     104     121     653    1422    2130    2196    2262    2850 
   Size:        104      17     532     769     708      66      66     588

Cuts at:   2850    3207    3228    3288    4176    4500    4670    4678    4689 
   Size:        357      21      60     888     324     170       8      11

Cuts at:   4689    5055    5634    6198    6285    6576    7959    8013 
   Size:        366     579     564      87     291    1383      54

  Fragments arranged by size: 

                1383     888     769     708     588     579     564     532
                 366     357     324     291     170     104      87      66
                  66      60      54      21      17      11       8
 
NaeI GCC'GGC

Cuts at:      0     615    2151    5179    6019    8013 
   Size:        615    1536    3028     840    1994

  Fragments arranged by size: 

                3028    1994    1536     840     615
 
NarI GG'CG_CC

Cuts at:      0    3425    3882    8013 
   Size:       3425     457    4131

  Fragments arranged by size: 

                4131    3425     457
 
NcoI C'CATG_G

Cuts at:      0    2498    2777    3356    4589    4622    6938    7401    8013 
   Size:       2498     279     579    1233      33    2316     463     612

  Fragments arranged by size: 

                2498    2316    1233     612     579     463     279      33
            
 
NgoMIV G'CCGG_C

Cuts at:      0     613    2149    5177    6017    8013 
   Size:        613    1536    3028     840    1996

  Fragments arranged by size: 

                3028    1996    1536     840     613
 
NotI GC'GGCC_GC

Cuts at:      0    3153    8013 
   Size:       3153    4860
 
NspI r_CATG'y

Cuts at:      0     286     586    1656    2217    3132    3162    6573    7613 
   Size:        286     300    1070     561     915      30    3411    1040

Cuts at:   7613    7695    8013 
   Size:         82     318

  Fragments arranged by size: 

                3411    1070    1040     915     561     318     300     286
                  82      30
 
PasI CC'CwG_GG

Cuts at:      0    7233    8013 
   Size:       7233     780
 
PciI A'CATG_T

Cuts at:      0    1652    7691    8013 
   Size:       1652    6039     322

  Fragments arranged by size: 

                6039    1652     322
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1187    1425    2235    2262    2376    2757    4035    4754 
   Size:       1187     238     810      27     114     381    1278     719

Cuts at:   4754    5280    5820    6072    8013 
   Size:        526     540     252    1941

  Fragments arranged by size: 

                1941    1278    1187     810     719     540     526     381
                 252     238     114      27
 
PfoI T'CCnGG_A

Cuts at:      0    1478    1814    1889    2912    3524    3773    4883    5249 
   Size:       1478     336      75    1023     612     249    1110     366

Cuts at:   5249    5729    7322    7874    8013 
   Size:        480    1593     552     139

  Fragments arranged by size: 

                1593    1478    1110    1023     612     552     480     366
                 336     249     139      75
 
PmlI CAC'GTG

Cuts at:      0    1187    2084    2349    2355    6139    6969    8013 
   Size:       1187     897     265       6    3784     830    1044

  Fragments arranged by size: 

                3784    1187    1044     897     830     265       6
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210    1870    1902    7048    7080    8013 
   Size:        178      32    1660      32    5146      32     933

  Fragments arranged by size: 

                5146    1660     933     178      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     129    1597    2718    4956    5112    5658    7320    8013 
   Size:        129    1468    1121    2238     156     546    1662     693

  Fragments arranged by size: 

                2238    1662    1468    1121     693     546     156     129
            
 
PshAI GACnn'nnGTC

Cuts at:      0    1852    6988    8013 
   Size:       1852    5136    1025

  Fragments arranged by size: 

                5136    1852    1025
 
PspOMI G'GGCC_C

Cuts at:      0    2689    5384    5532    5847    6835    7752    8013 
   Size:       2689    2695     148     315     988     917     261

  Fragments arranged by size: 

                2695    2689     988     917     315     261     148
 
PspXI vC'TCGA_Gb

Cuts at:      0    4525    8013 
   Size:       4525    3488
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     949     981    1627    1659    4243    4275    7690    7722 
   Size:        949      32     646      32    2584      32    3415      32

Cuts at:   7722    8013 
   Size:        291

  Fragments arranged by size: 

                3415    2584     949     646     291      32      32      32
                  32
 
PstI C_TGCA'G

Cuts at:      0     272     281     785    2708    3227    3632    3698    4357 
   Size:        272       9     504    1923     519     405      66     659

Cuts at:   4357    4475    4508    4787    5009    5633    5666    5726    5972 
   Size:        118      33     279     222     624      33      60     246

Cuts at:   5972    6104    6176    6203    7276    8013 
   Size:        132      72      27    1073     737

  Fragments arranged by size: 

                1923    1073     737     659     624     519     504     405
                 279     272     246     222     132     118      72      66
                  60      33      33      27       9
 
PvuII CAG'CTG

Cuts at:      0     653    1422    2130    2196    2262    3228    3288    4500 
   Size:        653     769     708      66      66     966      60    1212

Cuts at:   4500    4670    4678    5055    5634    6198    6285    8013 
   Size:        170       8     377     579     564      87    1728

  Fragments arranged by size: 

                1728    1212     966     769     708     653     579     564
                 377     170      87      66      66      60       8
 
RsrII CG'GwC_CG

Cuts at:      0    2000    8013 
   Size:       2000    6013
 
SacI G_AGCT'C

Cuts at:      0     602    1952    7937    8013 
   Size:        602    1350    5985      76

  Fragments arranged by size: 

                5985    1350     602      76
 
SacII CC_GC'GG

Cuts at:      0     122    8013 
   Size:        122    7891
 
SalI G'TCGA_C

Cuts at:      0    5464    7195    8013 
   Size:       5464    1731     818

  Fragments arranged by size: 

                5464    1731     818
 
SapI GCTCTTCn'nnn_

Cuts at:      0     376     490    3238    4885    6104    6469    6730    8013 
   Size:        376     114    2748    1647    1219     365     261    1283

  Fragments arranged by size: 

                2748    1647    1283    1219     376     365     261     114
            
 
SbfI CC_TGCA'GG

Cuts at:      0    6104    8013 
   Size:       6104    1909
 
ScaI AGT'ACT

Cuts at:      0     211    1054    2059    2233    5839    8013 
   Size:        211     843    1005     174    3606    2174

  Fragments arranged by size: 

                3606    2174    1005     843     211     174
 
SexAI A'CCwGG_T

Cuts at:      0    1433    8013 
   Size:       1433    6580
 
SfcI C'TryA_G

Cuts at:      0     268     277     781     929    2184    2625    2704    3223 
   Size:        268       9     504     148    1255     441      79     519

Cuts at:   3223    3628    3694    4353    4471    4504    4540    4783    5005 
   Size:        405      66     659     118      33      36     243     222

Cuts at:   5005    5629    5662    5722    5968    6100    6172    6199    6774 
   Size:        624      33      60     246     132      72      27     575

Cuts at:   6774    7020    7272    8013 
   Size:        246     252     741

  Fragments arranged by size: 

                1255     741     659     624     575     519     504     441
                 405     268     252     246     246     243     222     148
                 132     118      79      72      66      60      36      33
                  33      27       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1022    8013 
   Size:       1022    6991
 
SfoI GGC'GCC

Cuts at:      0    3426    3883    8013 
   Size:       3426     457    4130

  Fragments arranged by size: 

                4130    3426     457
 
SmlI C'TyrA_G

Cuts at:      0    4525    5020    5974    6298    7171    7189    7600    7936 
   Size:       4525     495     954     324     873      18     411     336

Cuts at:   7936    8013 
   Size:         77

  Fragments arranged by size: 

                4525     954     873     495     411     336     324      77
                  18
 
SphI G_CATG'C

Cuts at:      0     286     586    3132    6573    8013 
   Size:        286     300    2546    3441    1440

  Fragments arranged by size: 

                3441    2546    1440     300     286
 
StuI AGG'CCT

Cuts at:      0    1282    2710    4570    4900    6094    8013 
   Size:       1282    1428    1860     330    1194    1919

  Fragments arranged by size: 

                1919    1860    1428    1282    1194     330
 
StyI C'CwwG_G

Cuts at:      0     989    1265    2498    2777    3356    3431    4388    4589 
   Size:        989     276    1233     279     579      75     957     201

Cuts at:   4589    4598    4622    4952    5819    6938    7401    7977    8013 
   Size:          9      24     330     867    1119     463     576      36

  Fragments arranged by size: 

                1233    1119     989     957     867     579     576     463
                 330     279     276     201      75      36      24       9
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1852    1957    2017    3369    3491    5185    6844    7756 
   Size:       1852     105      60    1352     122    1694    1659     912

Cuts at:   7756    8013 
   Size:        257

  Fragments arranged by size: 

                1852    1694    1659    1352     912     257     122     105
                  60
 
TatI w'GTAC_w

Cuts at:      0     209     767    1052    2057    2231    5837    7706    8013 
   Size:        209     558     285    1005     174    3606    1869     307

  Fragments arranged by size: 

                3606    1869    1005     558     307     285     209     174
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     704     736    2755    2787    4543    4575    4966    4998 
   Size:        704      32    2019      32    1756      32     391      32

Cuts at:   4998    5725    5757    6833    6865    8013 
   Size:        727      32    1076      32    1148

  Fragments arranged by size: 

                2019    1756    1148    1076     727     704     391      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      37    5400    7294    7656    8013 
   Size:         37    5363    1894     362     357

  Fragments arranged by size: 

                5363    1894     362     357      37
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1350    1839    2505    2754    3069    3108    4959    5277 
   Size:       1350     489     666     249     315      39    1851     318

Cuts at:   5277    5281    5406    5484    5703    5760    5997    6696    6822 
   Size:          4     125      78     219      57     237     699     126

Cuts at:   6822    7899    8013 
   Size:       1077     114

  Fragments arranged by size: 

                1851    1350    1077     699     666     489     318     315
                 249     237     219     126     125     114      78      57
                  39       4
 
XhoI C'TCGA_G

Cuts at:      0    4525    7189    8013 
   Size:       4525    2664     824

  Fragments arranged by size: 

                4525    2664     824
 
XmnI GAAnn'nnTTC

Cuts at:      0    2921    5245    5311    8013 
   Size:       2921    2324      66    2702

  Fragments arranged by size: 

                2921    2702    2324      66
 
ZraI GAC'GTC

Cuts at:      0    6081    8013 
   Size:       6081    1932
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AgeI     AscI     AseI    AsiSI     BaeI    BciVI    BfrBI
     BmtI    BsrGI    BstBI   BstEII  BstZ17I     ClaI     DraI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NruI
     NsiI     PacI     PmeI     PsiI     PvuI    SanDI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XmaI
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