################################## # NO CUT ENZYMES ( 44 enzymes) # ################################## Acc65I AgeI Alw44I Aor51HI ApaLI AscI Asp700I Asp718I AvrII BlnI BsiWI Bsp1407I BsrGI BssHII Bst1107I BstZ17I CpoI DrdI Eco47III FseI HpaI KpnI MfeI MluI MunI NdeI NheI NotI PacI PaeR7I PinAI PmeI PvuI RsrII SalI ScaI SgrAI SnaBI SpeI SspBI SspI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 3823 GACGTC GACGT^C AclI 2803 AACGTT AA^CGTT AflII 910 CTTAAG C^TTAAG AhdI 363 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 363 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4754 TGCGCA TGC^GCA BbeI 5494 GGCGCC GGCGC^C BcgI 2535 CGAGATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5211 GTATCC GTATCC(6/5) BfaI 6784 CTAG C^TAG BfrI 910 CTTAAG C^TTAAG BsmBI 7093 GAGACG CGTCTC(1/5) Eam1105I 363 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 5956 ATGCAT ATGCA^T FspI 4754 TGCGCA TGC^GCA HaeII 5494 GGCGCC [GA]GCGC^[CT] HincII 2620 GTTGAC GT[CT]^[GA]AC HindII 2620 GTTGAC GT[CT]^[GA]AC HindIII 1361 AAGCTT A^AGCTT KasI 5494 GGCGCC G^GCGCC KspI 3366 CCGCGG CCGC^GG MaeI 6784 CTAG C^TAG NarI 5494 GGCGCC GG^CGCC NsiI 5956 ATGCAT ATGCA^T PshAI 633 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2803 AACGTT AA^CGTT SacII 3366 CCGCGG CCGC^GG SbfI 7029 CCTGCAGG CCTGCA^GG SfiI 1131 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 5494 GGCGCC GGC^GCC SmaI 5450 CCCGGG CCC^GGG SphI 824 GCATGC GCATG^C Sse8387I 7029 CCTGCAGG CCTGCA^GG SwaI 5574 ATTTAAAT ATTT^AAAT XmaCI 5450 CCCGGG C^CCGGG XmaI 5450 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AocI 1142 CCTTAGG CC^T[ACGT]AGG 3465 CCTCAGG AseI 2448 ATTAAT AT^TAAT 3795 ATTAAT AsnI 2448 ATTAAT AT^TAAT 3795 ATTAAT AspI 7009 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 7225 GACTCTGTC BamHI 3362 GGATCC G^GATCC 5398 GGATCC BbrPI 5536 CACGTG CAC^GTG 7054 CACGTG BglI 1132 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1615 GCCCCGCTGGC BglII 5011 AGATCT A^GATCT 6726 AGATCT BlpI 3633 GCTGAGC GC^T[ACGT]AGC 7318 GCTGAGC Bpu1102I 3633 GCTGAGC GC^T[ACGT]AGC 7318 GCTGAGC BsaI 631 GAGACC GGTCTC(1/5) 2224 GAGACC BsmI 1533 GAATGC GAATGC(1/-1) 5462 GCATTC BspDI 3562 ATCGAT AT^CGAT 6751 ATCGAT BssSI 2077 CACGAG CACGAG(-5/-1) 551 CTCGTG BstBI 2142 TTCGAA TT^CGAA 4672 TTCGAA BstEII 894 GGTGACC G^GT[ACGT]ACC 5790 GGTGACC BstPI 894 GGTGACC G^GT[ACGT]ACC 5790 GGTGACC Bsu36I 1142 CCTTAGG CC^T[ACGT]AGG 3465 CCTCAGG CelII 3633 GCTGAGC GC^T[ACGT]AGC 7318 GCTGAGC ClaI 3562 ATCGAT AT^CGAT 6751 ATCGAT DraI 5185 TTTAAA TTT^AAA 5575 TTTAAA EagI 1802 CGGCCG C^GGCCG 6912 CGGCCG EclXI 1802 CGGCCG C^GGCCG 6912 CGGCCG Eco52I 1802 CGGCCG C^GGCCG 6912 CGGCCG Eco81I 1142 CCTTAGG CC^T[ACGT]AGG 3465 CCTCAGG EcoNI 404 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6999 CCTCCTGGAGG EcoO65I 894 GGTGACC G^GT[ACGT]ACC 5790 GGTGACC NruI 1554 TCGCGA TCG^CGA 6843 TCGCGA NspV 2142 TTCGAA TT^CGAA 4672 TTCGAA PflFI 7009 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 7225 GACTCTGTC PmaCI 5536 CACGTG CAC^GTG 7054 CACGTG PmlI 5536 CACGTG CAC^GTG 7054 CACGTG PshBI 2448 ATTAAT AT^TAAT 3795 ATTAAT SapI 4249 GAAGAGC GCTCTTC(1/4) 4056 GCTCTTC SfuI 2142 TTCGAA TT^CGAA 4672 TTCGAA Tth111I 7009 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 7225 GACTCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII