Restriction map for pF1KE5838
##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp718I     AvrII       BamHI       BbeI        BbvCI       
BcgI        BfrI        BlnI        BsiEI       BsiWI       
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
CpoI        EagI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     FseI        KasI        KpnI        
KspI        MluI        NarI        NheI        NotI        
NruI        PacI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SexAI       
SfoI        SgrAI       SmaI        SnaBI       SspI        
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI         1399  GTCGAC               GT^[AC][GT]AC
AgeI         2558  ACCGGT               A^CCGGT
Aor51HI      1730  AGCGCT               AGC^GCT
AviII        2300  TGCGCA               TGC^GCA
BanI         4608  GGTGCC               G^G[CT][GA]CC
BclI         3443  TGATCA               T^GATCA
Bsp1407I     1571  TGTACA               T^GTACA
BspDI        3360  ATCGAT               AT^CGAT
BsrGI        1571  TGTACA               T^GTACA
BssHII       4899  GCGCGC               G^CGCGC
BssSI        3502  CACGAG               CACGAG(-5/-1)
BstBI         926  TTCGAA               TT^CGAA
ClaI         3360  ATCGAT               AT^CGAT
DraI         4069  TTTAAA               TTT^AAA
DraIII       4656  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1730  AGCGCT               AGC^GCT
EcoRV        3363  GATATC               GAT^ATC
FbaI         3443  TGATCA               T^GATCA
FspI         2300  TGCGCA               TGC^GCA
HpaI          667  GTTAAC               GTT^AAC
MfeI         4682  CAATTG               C^AATTG
MunI         4682  CAATTG               C^AATTG
NdeI         4937  CATATG               CA^TATG
NspV          926  TTCGAA               TT^CGAA
PinAI        2558  ACCGGT               A^CCGGT
SalI         1399  GTCGAC               G^TCGAC
SbfI         3949  CCTGCAGG             CCTGCA^GG
ScaI         2816  AGTACT               AGT^ACT
SfuI          926  TTCGAA               TT^CGAA
SpeI         4102  ACTAGT               A^CTAGT
Sse8387I     3949  CCTGCAGG             CCTGCA^GG
SspBI        1571  TGTACA               T^GTACA
XbaI         4533  TCTAGA               T^CTAGA
XcmI         1991  CCAAGGCCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   45 enzymes) #
###################################

AccIII        128  TCCGGA               T^CCGGA
             1736  TCCGGA              
AcyI          269  GGCGTC               G[GA]^CG[CT]C
             3871  GACGCC              
AhdI          355  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5056  GACAGCCTGTC         
ApaI         1588  GGGCCC               GGGCC^C
             2982  GGGCCC              
AspEI         355  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5056  GACAGCCTGTC         
BbrPI        2199  CACGTG               CAC^GTG
             2250  CACGTG              
BciVI        2644  GGATAC               GTATCC(6/5)
             3650  GGATAC              
BcnI         3513  CCCGG                CC^[GC]GG
             4095  CCGGG               
BglI         1996  GCCCTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3271  GCCATCATGGC         
BglII        1418  AGATCT               A^GATCT
             4408  AGATCT              
BlpI         1222  GCTCAGC              GC^T[ACGT]AGC
             1376  GCTTAGC             
Bpu1102I     1222  GCTCAGC              GC^T[ACGT]AGC
             1376  GCTTAGC             
BsaAI        2199  CACGTG               [CT]AC^GT[GA]
             2250  CACGTG              
BsaBI        1665  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2712  GATCGTAATC          
BsaHI         269  GGCGTC               G[GA]^CG[CT]C
             3871  GACGCC              
BsaI         3205  GAGACC               GGTCTC(1/5)
             3961  GAGACC              
BseAI         128  TCCGGA               T^CCGGA
             1736  TCCGGA              
BsgI         3864  CTGCAC               GTGCAG(16/14)
             2253  GTGCAG              
BsmBI        2875  CGTCTC               CGTCTC(1/5)
             2947  CGTCTC              
BsmI         4441  GAATGC               GAATGC(1/-1)
              864  GCATTC              
BspEI         128  TCCGGA               T^CCGGA
             1736  TCCGGA              
BspLU11I     2276  ACATGT               A^CATGT
             3680  ACATGT              
BspMI        1637  ACCTGC               ACCTGC(4/8)
             3952  GCAGGT              
BsrBI        3843  CCGCTC               CCGCTC(-3/-3)
             3820  GAGCGG              
CelII        1222  GCTCAGC              GC^T[ACGT]AGC
             1376  GCTTAGC             
DrdI         2902  GACGGGAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4201  GACATCTATGTC        
Eam1105I      355  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5056  GACAGCCTGTC         
EcoRI        2333  GAATTC               G^AATTC
             2590  GAATTC              
EcoT22I       862  ATGCAT               ATGCA^T
             3591  ATGCAT              
FauI         1700  GCGGG                CCCGC(4/6)
             3822  GCGGG               
HaeII        1730  AGCGCT               [GA]GCGC^[CT]
             3803  AGCGCC              
HgaI         3871  GACGC                GACGC(5/10)
              270  GCGTC               
Hin1I         269  GGCGTC               G[GA]^CG[CT]C
             3871  GACGCC              
MamI         1665  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2712  GATCGTAATC          
MroI          128  TCCGGA               T^CCGGA
             1736  TCCGGA              
NaeI         2288  GCCGGC               GCC^GGC
             3267  GCCGGC              
NciI         3513  CCCGG                CC^[GC]GG
             4095  CCGGG               
NgoMIV       2288  GCCGGC               G^CCGGC
             3267  GCCGGC              
NsiI          862  ATGCAT               ATGCA^T
             3591  ATGCAT              
PaeR7I        725  CTCGAG               C^TCGAG
             3846  CTCGAG              
PmaCI        2199  CACGTG               CAC^GTG
             2250  CACGTG              
PmlI         2199  CACGTG               CAC^GTG
             2250  CACGTG              
SfiI         1995  GGCCCTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3270  GGCCATCATGGCC       
StuI         2036  AGGCCT               AGG^CCT
             2313  AGGCCT              
XhoI          725  CTCGAG               C^TCGAG
             3846  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AspI        AvaI        AvaII       BalI        
BanII       BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XhoII       
XmnI        
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