Restriction map for pF1KE5838
 (Linear) (Six-base) MAPSORT of: KIEE5838.seq  Check: 2228  from: 1  to: 5121



KIEE5838.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3943    5121 
   Size:       3943    1178
 
AccI GT'mk_AC

Cuts at:      0    1400    5121 
   Size:       1400    3721
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     301    2200    2877    3435    3591    3647    4403 
   Size:        193     108    1899     677     558     156      56     756

Cuts at:   4403    5121 
   Size:        718

  Fragments arranged by size: 

                1899     756     718     677     558     193     156     108
                  56
 
AfeI AGC'GCT

Cuts at:      0    1732    5121 
   Size:       1732    3389
 
AflIII A'CryG_T

Cuts at:      0    2249    2276    3680    5121 
   Size:       2249      27    1404    1441

  Fragments arranged by size: 

                2249    1441    1404      27
 
AgeI A'CCGG_T

Cuts at:      0    2558    5121 
   Size:       2558    2563
 
AhdI GACnn_n'nnGTC

Cuts at:      0     360    5061    5121 
   Size:        360    4701      60

  Fragments arranged by size: 

                4701     360      60
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2278    5121 
   Size:       2278    2843
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3610    3642    5121 
   Size:       3610      32    1479

  Fragments arranged by size: 

                3610    1479      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1230    2076    2265    3480    4147    5121 
   Size:       1230     846     189    1215     667     974

  Fragments arranged by size: 

                1230    1215     974     846     667     189
 
ApaI G_GGCC'C

Cuts at:      0    1592    2986    5121 
   Size:       1592    1394    2135

  Fragments arranged by size: 

                2135    1592    1394
 
ApoI r'AATT_y

Cuts at:      0      72    2333    2590    5121 
   Size:         72    2261     257    2531

  Fragments arranged by size: 

                2531    2261     257      72
 
AvaI C'yCGr_G

Cuts at:      0     725    3209    3846    5121 
   Size:        725    2484     637    1275

  Fragments arranged by size: 

                2484    1275     725     637
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4286    4319    5121 
   Size:       4286      33     802

  Fragments arranged by size: 

                4286     802      33
 
BanI G'GyrC_C

Cuts at:      0    4608    5121 
   Size:       4608     513
 
BanII G_rGCy'C

Cuts at:      0     620    1592    1916    2120    2486    2986    3563    4229 
   Size:        620     972     324     204     366     500     577     666

Cuts at:   4229    5121 
   Size:        892

  Fragments arranged by size: 

                 972     892     666     620     577     500     366     324
                 204
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     936    1201    3776    4188    5121 
   Size:        936     265    2575     412     933

  Fragments arranged by size: 

                2575     936     933     412     265
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2638    3644    5121 
   Size:       2638    1006    1477

  Fragments arranged by size: 

                2638    1477    1006
 
BclI T'GATC_A

Cuts at:      0    3443    5121 
   Size:       3443    1678
 
BfrBI ATG'CAT

Cuts at:      0     864    3593    5121 
   Size:        864    2729    1528

  Fragments arranged by size: 

                2729    1528     864
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2002    3277    5121 
   Size:       2002    1275    1844

  Fragments arranged by size: 

                2002    1844    1275
 
BglII A'GATC_T

Cuts at:      0    1418    4408    5121 
   Size:       1418    2990     713

  Fragments arranged by size: 

                2990    1418     713
 
BlpI GC'TnA_GC

Cuts at:      0    1223    1377    5121 
   Size:       1223     154    3744

  Fragments arranged by size: 

                3744    1223     154
 
Bme1580I G_kGCm'C

Cuts at:      0    1592    2986    5121 
   Size:       1592    1394    2135

  Fragments arranged by size: 

                2135    1592    1394
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     296    5121 
   Size:        296    4825
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568     662     680     940    1477    1539    2208    2505 
   Size:        568      94      18     260     537      62     669     297

Cuts at:   2505    3715    3721    3820    3838    4283    4727    5121 
   Size:       1210       6      99      18     445     444     394

  Fragments arranged by size: 

                1210     669     568     537     445     444     394     297
                 260      99      94      62      18      18       6
 
Bpu10I CC'TnA_GC

Cuts at:      0      41    4084    5121 
   Size:         41    4043    1037

  Fragments arranged by size: 

                4043    1037      41
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1836    2302    3547    4566    4661    5121 
   Size:        580    1256     466    1245    1019      95     460

  Fragments arranged by size: 

                1256    1245    1019     580     466     460      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3199    3955    5121 
   Size:       3199     756    1166

  Fragments arranged by size: 

                3199    1166     756
 
BsaAI yAC'GTr

Cuts at:      0    2201    2252    5121 
   Size:       2201      51    2869

  Fragments arranged by size: 

                2869    2201      51
 
BsaBI GATnn'nnATC

Cuts at:      0    1669    2716    5121 
   Size:       1669    1047    2405

  Fragments arranged by size: 

                2405    1669    1047
 
BsaHI Gr'CG_yC

Cuts at:      0     270    3872    5121 
   Size:        270    3602    1249

  Fragments arranged by size: 

                3602    1249     270
 
BsaWI w'CCGG_w

Cuts at:      0     128    1736    2558    5121 
   Size:        128    1608     822    2563

  Fragments arranged by size: 

                2563    1608     822     128
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     479     509     902     932    3667    3697    3952    3982 
   Size:        479      30     393      30    2735      30     255      30

Cuts at:   3982    4378    4408    4709    4739    5121 
   Size:        396      30     301      30     382

  Fragments arranged by size: 

                2735     479     396     393     382     301     255      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      95     487    1993    3115    3310    3973    4807    4936 
   Size:         95     392    1506    1122     195     663     834     129

Cuts at:   4936    5085    5121 
   Size:        149      36

  Fragments arranged by size: 

                1506    1122     834     663     392     195     149     129
                  95      36
 
BseYI C'CCAG_C

Cuts at:      0     235    1791    4275    4777    5121 
   Size:        235    1556    2484     502     344

  Fragments arranged by size: 

                2484    1556     502     344     235
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2274    3849    5121 
   Size:       2274    1575    1272

  Fragments arranged by size: 

                2274    1575    1272
 
BsiHKAI G_wGCw'C

Cuts at:      0    1916    2120    2486    3563    3719    4229    5121 
   Size:       1916     204     366    1077     156     510     892

  Fragments arranged by size: 

                1916    1077     892     510     366     204     156
 
BsmI GAATG_Cn'

Cuts at:      0     864    4447    5121 
   Size:        864    3583     674

  Fragments arranged by size: 

                3583     864     674
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2881    2953    5121 
   Size:       2881      72    2168

  Fragments arranged by size: 

                2881    2168      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     620    1592    1916    2120    2486    2986    3563    3719 
   Size:        620     972     324     204     366     500     577     156

Cuts at:   3719    4229    5121 
   Size:        510     892

  Fragments arranged by size: 

                 972     892     620     577     510     500     366     324
                 204     156
 
BspEI T'CCGG_A

Cuts at:      0     128    1736    5121 
   Size:        128    1608    3385

  Fragments arranged by size: 

                3385    1608     128
 
BspHI T'CATG_A

Cuts at:      0      86    1934    2031    3404    5121 
   Size:         86    1848      97    1373    1717

  Fragments arranged by size: 

                1848    1717    1373      97      86
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1646    3943    5121 
   Size:       1646    2297    1178

  Fragments arranged by size: 

                2297    1646    1178
 
BsrBI CCG'CTC

Cuts at:      0    3822    3845    5121 
   Size:       3822      23    1276

  Fragments arranged by size: 

                3822    1276      23
 
BsrDI GCAATG_nn'

Cuts at:      0      35     638    2498    3323    3595    4823    5121 
   Size:         35     603    1860     825     272    1228     298

  Fragments arranged by size: 

                1860    1228     825     603     298     272      35
 
BsrFI r'CCGG_y

Cuts at:      0    1443    2288    2558    3267    5121 
   Size:       1443     845     270     709    1854

  Fragments arranged by size: 

                1854    1443     845     709     270
 
BsrGI T'GTAC_A

Cuts at:      0    1571    5121 
   Size:       1571    3550
 
BssHII G'CGCG_C

Cuts at:      0    4899    5121 
   Size:       4899     222
 
BssSI C'ACGA_G

Cuts at:      0    3502    5121 
   Size:       3502    1619
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2015    4147    4342    5121 
   Size:       2015    2132     195     779

  Fragments arranged by size: 

                2132    2015     779     195
 
BstBI TT'CG_AA

Cuts at:      0     927    5121 
   Size:        927    4194
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      11     289     439    1998    3798    4158    5121 
   Size:         11     278     150    1559    1800     360     963

  Fragments arranged by size: 

                1800    1559     963     360     278     150      11
 
BstYI r'GATC_y

Cuts at:      0    1418    1450    4408    5121 
   Size:       1418      32    2958     713

  Fragments arranged by size: 

                2958    1418     713      32
 
BtgI C'CryG_G

Cuts at:      0      10     558    1985    2024    2216    3743    3791    4397 
   Size:         10     548    1427      39     192    1527      48     606

Cuts at:   4397    5121 
   Size:        724

  Fragments arranged by size: 

                1527    1427     724     606     548     192      48      39
                  10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     710    1389    2226    2823    5121 
   Size:        710     679     837     597    2298

  Fragments arranged by size: 

                2298     837     710     679     597
 
ClaI AT'CG_AT

Cuts at:      0    3361    5121 
   Size:       3361    1760
 
DraI TTT'AAA

Cuts at:      0    4071    5121 
   Size:       4071    1050
 
DraIII CAC_nnn'GTG

Cuts at:      0    4661    5121 
   Size:       4661     460
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2908    4207    5121 
   Size:       2908    1299     914

  Fragments arranged by size: 

                2908    1299     914
 
EaeI y'GGCC_r

Cuts at:      0     441     808    1441    1988    2100    2153    2692    3119 
   Size:        441     367     633     547     112      53     539     427

Cuts at:   3119    3269    3788    4160    5121 
   Size:        150     519     372     961

  Fragments arranged by size: 

                 961     633     547     539     519     441     427     372
                 367     150     112      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     789    1762    1847    2465    2531    2662    3547    3578 
   Size:        789     973      85     618      66     131     885      31

Cuts at:   3578    4532    4948    5121 
   Size:        954     416     173

  Fragments arranged by size: 

                 973     954     885     789     618     416     173     131
                  85      66      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1160    5121 
   Size:       1160    3961
 
EcoICRI GAG'CTC

Cuts at:      0    1914    2118    2484    3561    4227    5121 
   Size:       1914     204     366    1077     666     894

  Fragments arranged by size: 

                1914    1077     894     666     366     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     301     568     662     680     940    1477    1539 
   Size:        193     108     267      94      18     260     537      62

Cuts at:   1539    2200    2208    2505    2877    3435    3591    3647    3715 
   Size:        661       8     297     372     558     156      56      68

Cuts at:   3715    3721    3820    3838    4283    4403    4727    5121 
   Size:          6      99      18     445     120     324     394

  Fragments arranged by size: 

                 661     558     537     445     394     372     324     297
                 267     260     193     156     120     108      99      94
                  68      62      56      18      18       8       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     564     783    1018    1589    1995    3851    4079    5121 
   Size:        564     219     235     571     406    1856     228    1042

  Fragments arranged by size: 

                1856    1042     571     564     406     235     228     219
            
 
EcoRI G'AATT_C

Cuts at:      0    2333    2590    5121 
   Size:       2333     257    2531

  Fragments arranged by size: 

                2531    2333     257
 
EcoRV GAT'ATC

Cuts at:      0    3365    5121 
   Size:       3365    1756
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     621    3103    3147    4028    5121 
   Size:        621    2482      44     881    1093

  Fragments arranged by size: 

                2482    1093     881     621      44
 
FspI TGC'GCA

Cuts at:      0    2302    5121 
   Size:       2302    2819
 
HaeII r_GCGC'y

Cuts at:      0    1734    3807    5121 
   Size:       1734    2073    1314

  Fragments arranged by size: 

                2073    1734    1314
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     206     238     346     378     700     732     788     820 
   Size:        206      32     108      32     322      32      56      32

Cuts at:    820    1388    1420    2859    2891    3091    3123    3171    3203 
   Size:        568      32    1439      32     200      32      48      32

Cuts at:   3203    3245    3277    3667    3699    3952    3984    4378    4393 
   Size:         42      32     390      32     253      32     394      15

Cuts at:   4393    4410    4425    5047    5079    5121 
   Size:         17      15     622      32      42

  Fragments arranged by size: 

                1439     622     568     394     390     322     253     206
                 200     108      56      48      42      42      32      32
                  32      32      32      32      32      32      32      32
                  32      32      17      15      15
 
HincII GTy'rAC

Cuts at:      0     669    1401    2058    3315    3705    5121 
   Size:        669     732     657    1257     390    1416

  Fragments arranged by size: 

                1416    1257     732     669     657     390
 
HindIII A'AGCT_T

Cuts at:      0     541    4287    5100    5121 
   Size:        541    3746     813      21

  Fragments arranged by size: 

                3746     813     541      21
 
HpaI GTT'AAC

Cuts at:      0     669    5121 
   Size:        669    4452
 
MfeI C'AATT_G

Cuts at:      0    4682    5121 
   Size:       4682     439
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     509    1451    2841    3392    3857    4006    4037 
   Size:        473      36     942    1390     551     465     149      31

Cuts at:   4037    4895    5121 
   Size:        858     226

  Fragments arranged by size: 

                1390     942     858     551     473     465     226     149
                  36      31
 
MscI TGG'CCA

Cuts at:      0     443    1990    2102    2155    3121    3790    4162    5121 
   Size:        443    1547     112      53     966     669     372     959

  Fragments arranged by size: 

                1547     966     959     669     443     372     112      53
            
 
MslI CAynn'nnrTG

Cuts at:      0     463    1243    2275    2281    2491    3040    3463    3685 
   Size:        463     780    1032       6     210     549     423     222

Cuts at:   3685    4191    4396    5121 
   Size:        506     205     725

  Fragments arranged by size: 

                1032     780     725     549     506     463     423     222
                 210     205       6
 
MspA1I CmG'CkG

Cuts at:      0    1176    2106    2151    3234    3765    4989    5121 
   Size:       1176     930      45    1083     531    1224     132

  Fragments arranged by size: 

                1224    1176    1083     930     531     132      45
 
NaeI GCC'GGC

Cuts at:      0    2290    3269    5121 
   Size:       2290     979    1852

  Fragments arranged by size: 

                2290    1852     979
 
NcoI C'CATG_G

Cuts at:      0     558    1985    2024    4397    5121 
   Size:        558    1427      39    2373     724

  Fragments arranged by size: 

                2373    1427     724     558      39
 
NdeI CA'TA_TG

Cuts at:      0    4938    5121 
   Size:       4938     183
 
NgoMIV G'CCGG_C

Cuts at:      0    2288    3267    5121 
   Size:       2288     979    1854

  Fragments arranged by size: 

                2288    1854     979
 
NsiI A_TGCA'T

Cuts at:      0     866    3595    5121 
   Size:        866    2729    1526

  Fragments arranged by size: 

                2729    1526     866
 
NspI r_CATG'y

Cuts at:      0     289     864    1536    2280    2301    2388    3141    3684 
   Size:        289     575     672     744      21      87     753     543

Cuts at:   3684    5121 
   Size:       1437

  Fragments arranged by size: 

                1437     753     744     672     575     543     289      87
                  21
 
PasI CC'CwG_GG

Cuts at:      0     758    2736    4454    5121 
   Size:        758    1978    1718     667

  Fragments arranged by size: 

                1978    1718     758     667
 
PciI A'CATG_T

Cuts at:      0    2276    3680    5121 
   Size:       2276    1404    1441

  Fragments arranged by size: 

                2276    1441    1404
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     521    1179    3744    5121 
   Size:        521     658    2565    1377

  Fragments arranged by size: 

                2565    1377     658     521
 
PfoI T'CCnGG_A

Cuts at:      0     187    2120    2521    5121 
   Size:        187    1933     401    2600

  Fragments arranged by size: 

                2600    1933     401     187
 
PmlI CAC'GTG

Cuts at:      0    2201    2252    5121 
   Size:       2201      51    2869

  Fragments arranged by size: 

                2869    2201      51
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1206    1238    5121 
   Size:       1206      32    3883

  Fragments arranged by size: 

                3883    1206      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     783    1018    3851    4079    5121 
   Size:        783     235    2833     228    1042

  Fragments arranged by size: 

                2833    1042     783     235     228
 
PsiI TTA'TAA

Cuts at:      0     209    5121 
   Size:        209    4912
 
PspOMI G'GGCC_C

Cuts at:      0    1588    2982    5121 
   Size:       1588    1394    2139

  Fragments arranged by size: 

                2139    1588    1394
 
PspXI vC'TCGA_Gb

Cuts at:      0    3846    5121 
   Size:       3846    1275
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2917    2949    3118    3150    5121 
   Size:       2917      32     169      32    1971

  Fragments arranged by size: 

                2917    1971     169      32      32
 
PstI C_TGCA'G

Cuts at:      0     572    2210    2813    3954    4143    5121 
   Size:        572    1638     603    1141     189     978

  Fragments arranged by size: 

                1638    1141     978     603     572     189
 
PvuII CAG'CTG

Cuts at:      0    1176    2106    2151    3234    3765    5121 
   Size:       1176     930      45    1083     531    1356

  Fragments arranged by size: 

                1356    1176    1083     930     531      45
 
SacI G_AGCT'C

Cuts at:      0    1916    2120    2486    3563    4229    5121 
   Size:       1916     204     366    1077     666     892

  Fragments arranged by size: 

                1916    1077     892     666     366     204
 
SalI G'TCGA_C

Cuts at:      0    1399    5121 
   Size:       1399    3722
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2662    3578    4948    5121 
   Size:       2662     916    1370     173

  Fragments arranged by size: 

                2662    1370     916     173
 
SbfI CC_TGCA'GG

Cuts at:      0    3954    5121 
   Size:       3954    1167
 
ScaI AGT'ACT

Cuts at:      0    2818    5121 
   Size:       2818    2303
 
SfcI C'TryA_G

Cuts at:      0     568    1650    2206    2788    2809    3950    4115    4139 
   Size:        568    1082     556     582      21    1141     165      24

Cuts at:   4139    5121 
   Size:        982

  Fragments arranged by size: 

                1141    1082     982     582     568     556     165      24
                  21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2002    3277    5121 
   Size:       2002    1275    1844

  Fragments arranged by size: 

                2002    1844    1275
 
SmlI C'TyrA_G

Cuts at:      0     595     725    1815    2317    3562    3846    4581    4676 
   Size:        595     130    1090     502    1245     284     735      95

Cuts at:   4676    5121 
   Size:        445

  Fragments arranged by size: 

                1245    1090     735     595     502     445     284     130
                  95
 
SpeI A'CTAG_T

Cuts at:      0    4102    5121 
   Size:       4102    1019
 
SphI G_CATG'C

Cuts at:      0     864    1536    2301    5121 
   Size:        864     672     765    2820

  Fragments arranged by size: 

                2820     864     765     672
 
StuI AGG'CCT

Cuts at:      0    2038    2315    5121 
   Size:       2038     277    2806

  Fragments arranged by size: 

                2806    2038     277
 
StyI C'CwwG_G

Cuts at:      0     432     515     558    1985    1991    2024    4157    4269 
   Size:        432      83      43    1427       6      33    2133     112

Cuts at:   4269    4397    5121 
   Size:        128     724

  Fragments arranged by size: 

                2133    1427     724     432     128     112      83      43
                  33       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     967    2619    2716    4011    4362    4444    5121 
   Size:        967    1652      97    1295     351      82     677

  Fragments arranged by size: 

                1652    1295     967     677     351      97      82
 
TatI w'GTAC_w

Cuts at:      0    1571    2816    3026    3570    5121 
   Size:       1571    1245     210     544    1551

  Fragments arranged by size: 

                1571    1551    1245     544     210
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     902     934    1010    1042    4709    4741    5121 
   Size:        902      32      76      32    3667      32     380

  Fragments arranged by size: 

                3667     902     380      76      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     822    3855    3966    5121 
   Size:        822    3033     111    1155

  Fragments arranged by size: 

                3033    1155     822     111
 
XbaI T'CTAG_A

Cuts at:      0    4533    5121 
   Size:       4533     588
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1998    5121 
   Size:       1998    3123
 
XhoI C'TCGA_G

Cuts at:      0     725    3846    5121 
   Size:        725    3121    1275

  Fragments arranged by size: 

                3121    1275     725
 
XmnI GAAnn'nnTTC

Cuts at:      0    1198    3130    3541    3922    4008    4957    5121 
   Size:       1198    1932     411     381      86     949     164

  Fragments arranged by size: 

                1932    1198     949     411     381     164      86
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI     BaeI     BanI    BanII
     BbsI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI    PvuII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfiI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AleI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI    BmgBI     BmtI
     BplI    BsiEI    BsiWI   BstEII  BstZ17I   Bsu36I     BtsI    CspCI
     EagI    EcoNI     FseI    FspAI     KasI     KpnI     MluI     NarI
     NheI     NotI     NruI     PacI     PmeI    PshAI     PvuI    RsrII
    SacII    SanDI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SrfI     SspI     SwaI     XmaI     ZraI
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