Restriction map for pF1KE2530
##################################
# NO CUT ENZYMES (   33 enzymes) #
##################################

AflII       Aor51HI     AscI        AviII       BbeI        
BfrI        BsiWI       CpoI        Eco47III    EcoRV       
FseI        FspI        HaeII       KasI        KspI        
MluI        NarI        NheI        NruI        PacI        
PmeI        PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         2187  GTATAC               GT^[AC][GT]AC
AclI         2824  AACGTT               AA^CGTT
AgeI         8839  ACCGGT               A^CCGGT
AocI         6994  CCTCAGG              CC^T[ACGT]AGG
ApaI         4880  GGGCCC               GGGCC^C
AseI         1135  ATTAAT               AT^TAAT
AsnI         1135  ATTAAT               AT^TAAT
BbrPI        4638  CACGTG               CAC^GTG
BciVI        4360  GGATAC               GTATCC(6/5)
BlpI         3568  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3568  GCTGAGC              GC^T[ACGT]AGC
BsiEI          70  CGGCCG               CG[GA][CT]^CG
Bsp1407I     3268  TGTACA               T^GTACA
BspDI        4299  ATCGAT               AT^CGAT
BsrBI         710  CCGCTC               CCGCTC(-3/-3)
BsrGI        3268  TGTACA               T^GTACA
BssHII       1754  GCGCGC               G^CGCGC
Bst1107I     2187  GTATAC               GTA^TAC
BstBI        6464  TTCGAA               TT^CGAA
BstEII       8666  GGTTACC              G^GT[ACGT]ACC
BstPI        8666  GGTTACC              G^GT[ACGT]ACC
BstZ17I      2187  GTATAC               GTA^TAC
Bsu36I       6994  CCTCAGG              CC^T[ACGT]AGG
CelII        3568  GCTGAGC              GC^T[ACGT]AGC
ClaI         4299  ATCGAT               AT^CGAT
DraI         5493  TTTAAA               TTT^AAA
DrdI          799  GACCCCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           70  CGGCCG               C^GGCCG
EclXI          70  CGGCCG               C^GGCCG
Eco52I         70  CGGCCG               C^GGCCG
Eco81I       6994  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      8666  GGTTACC              G^GT[ACGT]ACC
NcoI         8078  CCATGG               C^CATGG
NotI           69  GCGGCCGC             GC^GGCCGC
NspV         6464  TTCGAA               TT^CGAA
PinAI        8839  ACCGGT               A^CCGGT
PmaCI        4638  CACGTG               CAC^GTG
PmlI         4638  CACGTG               CAC^GTG
PshBI        1135  ATTAAT               AT^TAAT
Psp1406I     2824  AACGTT               AA^CGTT
SapI         7123  GCTCTTC              GCTCTTC(1/4)
ScaI         4511  AGTACT               AGT^ACT
SfuI         6464  TTCGAA               TT^CGAA
SspBI        3268  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AatII        2302  GACGTC               GACGT^C
             6786  GACGTC              
Acc65I       6686  GGTACC               G^GTACC
             7070  GGTACC              
AccIII         40  TCCGGA               T^CCGGA
             3545  TCCGGA              
AcyI         2302  GACGTC               G[GA]^CG[CT]C
             6786  GACGTC              
AhdI         2638  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8238  GACACATGGTC         
Asp718I      6686  GGTACC               G^GTACC
             7070  GGTACC              
AspEI        2638  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8238  GACACATGGTC         
AspI         4618  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6220  GACAGCGTC           
AvrII        2218  CCTAGG               C^CTAGG
             7720  CCTAGG              
BalI         3888  TGGCCA               TGG^CCA
             8532  TGGCCA              
BbvCI        1690  CCTCAGC              CCTCAGC(-5/-2)
             4873  CCTCAGC             
BglI           47  GCCTCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3443  GCCGGCCTGGC         
BglII        3678  AGATCT               A^GATCT
             5838  AGATCT              
BlnI         2218  CCTAGG               C^CTAGG
             7720  CCTAGG              
BsaHI        2302  GACGTC               G[GA]^CG[CT]C
             6786  GACGTC              
BsaI          663  GAGACC               GGTCTC(1/5)
             7148  GAGACC              
BseAI          40  TCCGGA               T^CCGGA
             3545  TCCGGA              
BspEI          40  TCCGGA               T^CCGGA
             3545  TCCGGA              
BspMI        1925  ACCTGC               ACCTGC(4/8)
              393  GCAGGT              
BssSI        7145  CACGAG               CACGAG(-5/-1)
             4927  CTCGTG              
Eam1105I     2638  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8238  GACACATGGTC         
EcoT22I      3087  ATGCAT               ATGCA^T
             7043  ATGCAT              
HgaI         6224  GCGTC                GACGC(5/10)
             8726  GCGTC               
Hin1I        2302  GACGTC               G[GA]^CG[CT]C
             6786  GACGTC              
HpaI         2107  GTTAAC               GTT^AAC
             2233  GTTAAC              
KpnI         6686  GGTACC               GGTAC^C
             7070  GGTACC              
MluNI        3888  TGGCCA               TGG^CCA
             8532  TGGCCA              
MroI           40  TCCGGA               T^CCGGA
             3545  TCCGGA              
MscI         3888  TGGCCA               TGG^CCA
             8532  TGGCCA              
NaeI         1758  GCCGGC               GCC^GGC
             3443  GCCGGC              
NgoMIV       1758  GCCGGC               G^CCGGC
             3443  GCCGGC              
NsiI         3087  ATGCAT               ATGCA^T
             7043  ATGCAT              
PaeR7I       3953  CTCGAG               C^TCGAG
             5114  CTCGAG              
PflFI        4618  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6220  GACAGCGTC           
SmaI         5057  CCCGGG               CCC^GGG
             5282  CCCGGG              
Tth111I      4618  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6220  GACAGCGTC           
XbaI         5196  TCTAGA               T^CTAGA
             6810  TCTAGA              
XhoI         3953  CTCGAG               C^TCGAG
             5114  CTCGAG              
XmaCI        5057  CCCGGG               C^CCGGG
             5282  CCCGGG              
XmaI         5057  CCCGGG               C^CCGGG
             5282  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        Asp700I     AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbsI        BbvI        
BcgI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaBI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspLU11I    BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MfeI        MflI        MnlI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NciI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RcaI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SphI        SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmnI        
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