Restriction map for pF1KE2530
 (Linear) (Six-base) MAPSORT of: KIEE2530.seq  Check: 4155  from: 1  to: 9366



KIEE2530.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     384    9366 
   Size:        384    8982
 
AatII G_ACGT'C

Cuts at:      0    2306    6790    9366 
   Size:       2306    4484    2576

  Fragments arranged by size: 

                4484    2576    2306
 
AccI GT'mk_AC

Cuts at:      0    2188    9366 
   Size:       2188    7178
 
Acc65I G'GTAC_C

Cuts at:      0    6686    7070    9366 
   Size:       6686     384    2296

  Fragments arranged by size: 

                6686    2296     384
 
AclI AA'CG_TT

Cuts at:      0    2825    9366 
   Size:       2825    6541
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1070    1176    1958    2076    2423    3548    3583    5134 
   Size:       1070     106     782     118     347    1125      35    1551

Cuts at:   5134    5240    5896    5907    6214    6431    7486    8457    9292 
   Size:        106     656      11     307     217    1055     971     835

Cuts at:   9292    9366 
   Size:         74

  Fragments arranged by size: 

                1551    1125    1070    1055     971     835     782     656
                 347     307     217     118     106     106      74      35
                  11
 
AflIII A'CryG_T

Cuts at:      0     169     213     937    1174    1854    6645    6882    8797 
   Size:        169      44     724     237     680    4791     237    1915

Cuts at:   8797    9366 
   Size:        569

  Fragments arranged by size: 

                4791    1915     724     680     569     237     237     169
                  44
 
AgeI A'CCGG_T

Cuts at:      0    8839    9366 
   Size:       8839     527
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2643    8243    9366 
   Size:       2643    5600    1123

  Fragments arranged by size: 

                5600    2643    1123
 
AleI CACnn'nnGTG

Cuts at:      0    2339    9366 
   Size:       2339    7027
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     521    3890    4706    9366 
   Size:        521    3369     816    4660

  Fragments arranged by size: 

                4660    3369     816     521
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1844    1876    8598    8630    9366 
   Size:       1844      32    6722      32     736

  Fragments arranged by size: 

                6722    1844     736      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2158    3494    5071    9366 
   Size:       2158    1336    1577    4295

  Fragments arranged by size: 

                4295    2158    1577    1336
 
ApaI G_GGCC'C

Cuts at:      0    4884    9366 
   Size:       4884    4482
 
ApaLI G'TGCA_C

Cuts at:      0    3131    3399    4731    9366 
   Size:       3131     268    1332    4635

  Fragments arranged by size: 

                4635    3131    1332     268
 
ApoI r'AATT_y

Cuts at:      0     700    2017    2037    3250    3454    4038    5038    5136 
   Size:        700    1317      20    1213     204     584    1000      98

Cuts at:   5136    6300    6568    6948    7286    7499    8002    8576    8893 
   Size:       1164     268     380     338     213     503     574     317

Cuts at:   8893    8899    9366 
   Size:          6     467

  Fragments arranged by size: 

                1317    1213    1164    1000     700     584     574     503
                 467     380     338     317     268     213     204      98
                  20       6
 
AseI AT'TA_AT

Cuts at:      0    1136    9366 
   Size:       1136    8230
 
AvaI C'yCGr_G

Cuts at:      0      49    3782    3953    3974    5057    5114    5282    5876 
   Size:         49    3733     171      21    1083      57     168     594

Cuts at:   5876    9366 
   Size:       3490

  Fragments arranged by size: 

                3733    3490    1083     594     171     168      57      49
                  21
 
AvrII C'CTAG_G

Cuts at:      0    2218    7720    9366 
   Size:       2218    5502    1646

  Fragments arranged by size: 

                5502    2218    1646
 
BamHI G'GATC_C

Cuts at:      0     740    1318    1437    3196    9366 
   Size:        740     578     119    1759    6170

  Fragments arranged by size: 

                6170    1759     740     578     119
 
BanI G'GyrC_C

Cuts at:      0    1311    2957    4243    6686    7070    9366 
   Size:       1311    1646    1286    2443     384    2296

  Fragments arranged by size: 

                2443    2296    1646    1311    1286     384
 
BanII G_rGCy'C

Cuts at:      0    2104    2699    4884    6173    6741    7178    7216    7616 
   Size:       2104     595    2185    1289     568     437      38     400

Cuts at:   7616    9116    9366 
   Size:       1500     250

  Fragments arranged by size: 

                2185    2104    1500    1289     595     568     437     400
                 250      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2356    2411    3986    5576    5902    5981    6437    7349 
   Size:       2356      55    1575    1590     326      79     456     912

Cuts at:   7349    9366 
   Size:       2017

  Fragments arranged by size: 

                2356    2017    1590    1575     912     456     326      79
                  55
 
BbvCI CC'TCA_GC

Cuts at:      0    1691    4874    9366 
   Size:       1691    3183    4492

  Fragments arranged by size: 

                4492    3183    1691
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3074    3108    6268    6302    8277    8311    9366 
   Size:       3074      34    3160      34    1975      34    1055

  Fragments arranged by size: 

                3160    3074    1975    1055      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    4354    9366 
   Size:       4354    5012
 
BclI T'GATC_A

Cuts at:      0     954    3384    3821    7829    9366 
   Size:        954    2430     437    4008    1537

  Fragments arranged by size: 

                4008    2430    1537     954     437
 
BfrBI ATG'CAT

Cuts at:      0    3089    7045    9366 
   Size:       3089    3956    2321

  Fragments arranged by size: 

                3956    3089    2321
 
BglI GCCn_nnn'nGGC

Cuts at:      0      53    3449    9366 
   Size:         53    3396    5917

  Fragments arranged by size: 

                5917    3396      53
 
BglII A'GATC_T

Cuts at:      0    3678    5838    9366 
   Size:       3678    2160    3528

  Fragments arranged by size: 

                3678    3528    2160
 
BlpI GC'TnA_GC

Cuts at:      0    3569    9366 
   Size:       3569    5797
 
Bme1580I G_kGCm'C

Cuts at:      0    2179    3000    3135    3403    4246    4530    4613    4735 
   Size:       2179     821     135     268     843     284      83     122

Cuts at:   4735    4884    6770    7190    7809    8133    9366 
   Size:        149    1886     420     619     324    1233

  Fragments arranged by size: 

                2179    1886    1233     843     821     619     420     324
                 284     268     149     135     122      83
 
BmgBI CAC'GTC

Cuts at:      0    3551    4096    6646    9366 
   Size:       3551     545    2550    2720

  Fragments arranged by size: 

                3551    2720    2550     545
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     332     566    3089    3401    3513    6719    6863    8224 
   Size:        332     234    2523     312     112    3206     144    1361

Cuts at:   8224    9366 
   Size:       1142

  Fragments arranged by size: 

                3206    2523    1361    1142     332     312     234     144
                 112
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3697    9366 
   Size:       3697    5669
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    1008    1770    1846    2330    2594    2987    4100 
   Size:        671     337     762      76     484     264     393    1113

Cuts at:   4100    4580    4670    4786    6656    6779    7588    8153    8763 
   Size:        480      90     116    1870     123     809     565     610

Cuts at:   8763    8942    9322    9366 
   Size:        179     380      44

  Fragments arranged by size: 

                1870    1113     809     762     671     610     565     484
                 480     393     380     337     264     179     123     116
                  90      76      44
 
Bpu10I CC'TnA_GC

Cuts at:      0    1691    4874    7215    7595    8192    9366 
   Size:       1691    3183    2341     380     597    1174

  Fragments arranged by size: 

                3183    2341    1691    1174     597     380
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1914    3742    3800    6057    6376    7455    9202    9366 
   Size:       1914    1828      58    2257     319    1079    1747     164

  Fragments arranged by size: 

                2257    1914    1828    1747    1079     319     164      58
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     657    7142    9366 
   Size:        657    6485    2224

  Fragments arranged by size: 

                6485    2224     657
 
BsaAI yAC'GTr

Cuts at:      0    1105    4640    6883    9366 
   Size:       1105    3535    2243    2483

  Fragments arranged by size: 

                3535    2483    2243    1105
 
BsaBI GATnn'nnATC

Cuts at:      0     643     859    1702    7328    9366 
   Size:        643     216     843    5626    2038

  Fragments arranged by size: 

                5626    2038     843     643     216
 
BsaHI Gr'CG_yC

Cuts at:      0    2303    6787    9366 
   Size:       2303    4484    2579

  Fragments arranged by size: 

                4484    2579    2303
 
BsaWI w'CCGG_w

Cuts at:      0      40    2474    3545    4990    8839    9366 
   Size:         40    2434    1071    1445    3849     527

  Fragments arranged by size: 

                3849    2434    1445    1071     527      40
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     654     684    1112    1142    1844    1874    2711    2741 
   Size:        654      30     428      30     702      30     837      30

Cuts at:   2741    4095    4125    6777    6807    9366 
   Size:       1354      30    2652      30    2559

  Fragments arranged by size: 

                2652    2559    1354     837     702     654     428      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      13    1496    2187    3869    4103    5199    5617    6535 
   Size:         13    1483     691    1682     234    1096     418     918

Cuts at:   6535    6782    7351    7756    9366 
   Size:        247     569     405    1610

  Fragments arranged by size: 

                1682    1610    1483    1096     918     691     569     418
                 405     247     234      13
 
BseYI C'CCAG_C

Cuts at:      0    1667    1785    2857    4703    6769    8208    9040    9156 
   Size:       1667     118    1072    1846    2066    1439     832     116

Cuts at:   9156    9357    9366 
   Size:        201       9

  Fragments arranged by size: 

                2066    1846    1667    1439    1072     832     201     118
                 116       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579    1912    2561    2835    3015    3297    4464    4620 
   Size:        579    1333     649     274     180     282    1167     156

Cuts at:   4620    9366 
   Size:       4746

  Fragments arranged by size: 

                4746    1333    1167     649     579     282     274     180
                 156
 
BsiEI CG_ry'CG

Cuts at:      0      73    9366 
   Size:         73    9293
 
BsiHKAI G_wGCw'C

Cuts at:      0    2104    2699    3135    3403    3526    4356    4735    6173 
   Size:       2104     595     436     268     123     830     379    1438

Cuts at:   6173    7125    7178    7616    7995    9116    9366 
   Size:        952      53     438     379    1121     250

  Fragments arranged by size: 

                2104    1438    1121     952     830     595     438     436
                 379     379     268     250     123      53
 
BsmI GAATG_Cn'

Cuts at:      0     759    1060    1652    2320    6243    6461    8654    9366 
   Size:        759     301     592     668    3923     218    2193     712

  Fragments arranged by size: 

                3923    2193     759     712     668     592     301     218
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    5111    6794    8362    9366 
   Size:       5111    1683    1568    1004

  Fragments arranged by size: 

                5111    1683    1568    1004
 
Bsp1286I G_dGCh'C

Cuts at:      0    2104    2179    2699    3000    3135    3403    3526    4246 
   Size:       2104      75     520     301     135     268     123     720

Cuts at:   4246    4356    4530    4613    4735    4884    6173    6741    6770 
   Size:        110     174      83     122     149    1289     568      29

Cuts at:   6770    7125    7178    7190    7216    7616    7809    7995    8133 
   Size:        355      53      12      26     400     193     186     138

Cuts at:   8133    9116    9366 
   Size:        983     250

  Fragments arranged by size: 

                2104    1289     983     720     568     520     400     355
                 301     268     250     193     186     174     149     138
                 135     123     122     110      83      75      53      29
                  26      12
 
BspEI T'CCGG_A

Cuts at:      0      40    3545    9366 
   Size:         40    3505    5821

  Fragments arranged by size: 

                5821    3505      40
 
BspHI T'CATG_A

Cuts at:      0    4778    7163    7854    9366 
   Size:       4778    2385     691    1512

  Fragments arranged by size: 

                4778    2385    1512     691
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     384    1934    9366 
   Size:        384    1550    7432

  Fragments arranged by size: 

                7432    1550     384
 
BsrBI CCG'CTC

Cuts at:      0     712    9366 
   Size:        712    8654
 
BsrDI GCAATG_nn'

Cuts at:      0    1242    1326    2147    2607    3222    4408    5511    6272 
   Size:       1242      84     821     460     615    1186    1103     761

Cuts at:   6272    6836    8318    9366 
   Size:        564    1482    1048

  Fragments arranged by size: 

                1482    1242    1186    1103    1048     821     761     615
                 564     460      84
 
BsrFI r'CCGG_y

Cuts at:      0    1758    2684    2764    3443    6320    8839    9366 
   Size:       1758     926      80     679    2877    2519     527

  Fragments arranged by size: 

                2877    2519    1758     926     679     527      80
 
BsrGI T'GTAC_A

Cuts at:      0    3268    9366 
   Size:       3268    6098
 
BssHII G'CGCG_C

Cuts at:      0    1754    9366 
   Size:       1754    7612
 
BssSI C'ACGA_G

Cuts at:      0    4927    7145    9366 
   Size:       4927    2218    2221

  Fragments arranged by size: 

                4927    2221    2218
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1731    2731    3494    6980    9366 
   Size:       1731    1000     763    3486    2386

  Fragments arranged by size: 

                3486    2386    1731    1000     763
 
BstBI TT'CG_AA

Cuts at:      0    6465    9366 
   Size:       6465    2901
 
BstEII G'GTnAC_C

Cuts at:      0    8666    9366 
   Size:       8666     700
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    5986    7255    9324    9366 
   Size:       5986    1269    2069      42

  Fragments arranged by size: 

                5986    2069    1269      42
 
BstYI r'GATC_y

Cuts at:      0     740    1318    1437    3196    3678    5310    5838    6030 
   Size:        740     578     119    1759     482    1632     528     192

Cuts at:   6030    6252    6631    9265    9366 
   Size:        222     379    2634     101

  Fragments arranged by size: 

                2634    1759    1632     740     578     528     482     379
                 222     192     119     101
 
BstZ17I GTA'TAC

Cuts at:      0    2189    9366 
   Size:       2189    7177
 
Bsu36I CC'TnA_GG

Cuts at:      0    6995    9366 
   Size:       6995    2371
 
BtgI C'CryG_G

Cuts at:      0    3000    4492    4655    8078    9366 
   Size:       3000    1492     163    3423    1288

  Fragments arranged by size: 

                3423    3000    1492    1288     163
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1559    3099    9366 
   Size:       1559    1540    6267

  Fragments arranged by size: 

                6267    1559    1540
 
BtsI GCAGTG_nn'

Cuts at:      0     250    1151    1419    3403    4325    4600    5762    9023 
   Size:        250     901     268    1984     922     275    1162    3261

Cuts at:   9023    9083    9366 
   Size:         60     283

  Fragments arranged by size: 

                3261    1984    1162     922     901     283     275     268
                 250      60
 
ClaI AT'CG_AT

Cuts at:      0    4300    9366 
   Size:       4300    5066
 
DraI TTT'AAA

Cuts at:      0    5495    9366 
   Size:       5495    3871
 
DraIII CAC_nnn'GTG

Cuts at:      0     326     524    2530    9366 
   Size:        326     198    2006    6836

  Fragments arranged by size: 

                6836    2006     326     198
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     805    9366 
   Size:        805    8561
 
EaeI y'GGCC_r

Cuts at:      0      70    1647    3888    4218    8532    9366 
   Size:         70    1577    2241     330    4314     834

  Fragments arranged by size: 

                4314    2241    1577     834     330      70
 
EagI C'GGCC_G

Cuts at:      0      70    9366 
   Size:         70    9296
 
EarI CTCTTCn'nnn_

Cuts at:      0    1829    2038    2933    7130    7857    9366 
   Size:       1829     209     895    4197     727    1509

  Fragments arranged by size: 

                4197    1829    1509     895     727     209
 
EcoICRI GAG'CTC

Cuts at:      0    2102    2697    6171    7176    7614    9114    9366 
   Size:       2102     595    3474    1005     438    1500     252

  Fragments arranged by size: 

                3474    2102    1500    1005     595     438     252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    1008    1070    1176    1770    1846    1958    2076 
   Size:        671     337      62     106     594      76     112     118

Cuts at:   2076    2330    2423    2594    2987    3548    3583    4100    4580 
   Size:        254      93     171     393     561      35     517     480

Cuts at:   4580    4670    4786    5134    5240    5896    5907    6214    6431 
   Size:         90     116     348     106     656      11     307     217

Cuts at:   6431    6656    6779    7486    7588    8153    8457    8763    8942 
   Size:        225     123     707     102     565     304     306     179

Cuts at:   8942    9292    9322    9366 
   Size:        350      30      44

  Fragments arranged by size: 

                 707     671     656     594     565     561     517     480
                 393     350     348     337     307     306     304     254
                 225     217     179     171     123     118     116     112
                 106     106     102      93      90      76      62      44
                  35      30      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2390    4625    5734    8039    9366 
   Size:       2390    2235    1109    2305    1327

  Fragments arranged by size: 

                2390    2305    2235    1327    1109
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18     184    2861    4881    5361    5872    8751    9321 
   Size:         18     166    2677    2020     480     511    2879     570

Cuts at:   9321    9366 
   Size:         45

  Fragments arranged by size: 

                2879    2677    2020     570     511     480     166      45
                  18
 
EcoRI G'AATT_C

Cuts at:      0    5038    7286    8576    8899    9366 
   Size:       5038    2248    1290     323     467

  Fragments arranged by size: 

                5038    2248    1290     467     323
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    5926    8596    9366 
   Size:       5926    2670     770

  Fragments arranged by size: 

                5926    2670     770
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     759     791    1112    1144    1344    1376    1669    1701 
   Size:        759      32     321      32     200      32     293      32

Cuts at:   1701    2492    2524    2629    2661    3663    3695    3828    3860 
   Size:        791      32     105      32    1002      32     133      32

Cuts at:   3860    5178    5210    5614    5646    5653    5685    6616    6648 
   Size:       1318      32     404      32       7      32     931      32

Cuts at:   6648    6777    6809    6971    7003    7198    7230    8229    8261 
   Size:        129      32     162      32     195      32     999      32

Cuts at:   8261    8928    8960    9245    9277    9366 
   Size:        667      32     285      32      89

  Fragments arranged by size: 

                1318    1002     999     931     791     759     667     404
                 321     293     285     200     195     162     133     129
                 105      89      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32       7
 
HincII GTy'rAC

Cuts at:      0    1805    2109    2235    2413    4318    6516    9366 
   Size:       1805     304     126     178    1905    2198    2850

  Fragments arranged by size: 

                2850    2198    1905    1805     304     178     126
 
HindIII A'AGCT_T

Cuts at:      0    5653    5777    5919    7975    9366 
   Size:       5653     124     142    2056    1391

  Fragments arranged by size: 

                5653    2056    1391     142     124
 
HpaI GTT'AAC

Cuts at:      0    2109    2235    9366 
   Size:       2109     126    7131

  Fragments arranged by size: 

                7131    2109     126
 
KpnI G_GTAC'C

Cuts at:      0    6690    7074    9366 
   Size:       6690     384    2292

  Fragments arranged by size: 

                6690    2292     384
 
MfeI C'AATT_G

Cuts at:      0    1032    1253    3892    7310    7628    8324    8363    9366 
   Size:       1032     221    2639    3418     318     696      39    1003

  Fragments arranged by size: 

                3418    2639    1032    1003     696     318     221      39
            
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     828    6609    6621    8351    8624    9177    9366 
   Size:        828    5781      12    1730     273     553     189

  Fragments arranged by size: 

                5781    1730     828     553     273     189      12
 
MscI TGG'CCA

Cuts at:      0    3890    8534    9366 
   Size:       3890    4644     832

  Fragments arranged by size: 

                4644    3890     832
 
MslI CAynn'nnrTG

Cuts at:      0    1657    1949    2271    2339    2595    4260    4302    4688 
   Size:       1657     292     322      68     256    1665      42     386

Cuts at:   4688    5984    7090    7168    7253    9059    9366 
   Size:       1296    1106      78      85    1806     307

  Fragments arranged by size: 

                1806    1665    1657    1296    1106     386     322     307
                 292     256      85      78      68      42
 
MspA1I CmG'CkG

Cuts at:      0      69      84    2122    2158    2633    2993    3494    4120 
   Size:         69      15    2038      36     475     360     501     626

Cuts at:   4120    4478    4803    4878    4902    6091    6529    8173    8451 
   Size:        358     325      75      24    1189     438    1644     278

Cuts at:   8451    9172    9366 
   Size:        721     194

  Fragments arranged by size: 

                2038    1644    1189     721     626     501     475     438
                 360     358     325     278     194      75      69      36
                  24      15
 
NaeI GCC'GGC

Cuts at:      0    1760    3445    9366 
   Size:       1760    1685    5921

  Fragments arranged by size: 

                5921    1760    1685
 
NcoI C'CATG_G

Cuts at:      0    8078    9366 
   Size:       8078    1288
 
NdeI CA'TA_TG

Cuts at:      0     570    1821    3869    8050    9366 
   Size:        570    1251    2048    4181    1316

  Fragments arranged by size: 

                4181    2048    1316    1251     570
 
NgoMIV G'CCGG_C

Cuts at:      0    1758    3443    9366 
   Size:       1758    1685    5923

  Fragments arranged by size: 

                5923    1758    1685
 
NotI GC'GGCC_GC

Cuts at:      0      70    9366 
   Size:         70    9296
 
NsiI A_TGCA'T

Cuts at:      0    3091    7047    9366 
   Size:       3091    3956    2319

  Fragments arranged by size: 

                3956    3091    2319
 
NspI r_CATG'y

Cuts at:      0     173     217     941    1031    1178    1297    4181    4693 
   Size:        173      44     724      90     147     119    2884     512

Cuts at:   4693    7938    8801    9366 
   Size:       3245     863     565

  Fragments arranged by size: 

                3245    2884     863     724     565     512     173     147
                 119      90      44
 
PasI CC'CwG_GG

Cuts at:      0     220    3331    4149    4239    5031    9366 
   Size:        220    3111     818      90     792    4335

  Fragments arranged by size: 

                4335    3111     818     792     220      90
 
PciI A'CATG_T

Cuts at:      0     169     213     937    1174    8797    9366 
   Size:        169      44     724     237    7623     569

  Fragments arranged by size: 

                7623     724     569     237     169      44
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3079    3143    7067    9366 
   Size:       3079      64    3924    2299

  Fragments arranged by size: 

                3924    3079    2299      64
 
PfoI T'CCnGG_A

Cuts at:      0     966    5315    8633    9366 
   Size:        966    4349    3318     733

  Fragments arranged by size: 

                4349    3318     966     733
 
PmlI CAC'GTG

Cuts at:      0    4640    9366 
   Size:       4640    4726
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1844    1876    2810    2842    9366 
   Size:       1844      32     934      32    6524

  Fragments arranged by size: 

                6524    1844     934      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      18     184    2861    5361    5872    9366 
   Size:         18     166    2677    2500     511    3494

  Fragments arranged by size: 

                3494    2677    2500     511     166      18
 
PshAI GACnn'nnGTC

Cuts at:      0    1490    2477    8120    9366 
   Size:       1490     987    5643    1246

  Fragments arranged by size: 

                5643    1490    1246     987
 
PspOMI G'GGCC_C

Cuts at:      0    4880    9366 
   Size:       4880    4486
 
PspXI vC'TCGA_Gb

Cuts at:      0    3953    9366 
   Size:       3953    5413
 
PstI C_TGCA'G

Cuts at:      0    1414    3187    3490    3608    5241    5769    8259    9366 
   Size:       1414    1773     303     118    1633     528    2490    1107

  Fragments arranged by size: 

                2490    1773    1633    1414    1107     528     303     118
            
 
PvuII CAG'CTG

Cuts at:      0    2158    2633    3494    4120    4902    6091    6529    8173 
   Size:       2158     475     861     626     782    1189     438    1644

Cuts at:   8173    8451    9172    9366 
   Size:        278     721     194

  Fragments arranged by size: 

                2158    1644    1189     861     782     721     626     475
                 438     278     194
 
SacI G_AGCT'C

Cuts at:      0    2104    2699    6173    7178    7616    9116    9366 
   Size:       2104     595    3474    1005     438    1500     250

  Fragments arranged by size: 

                3474    2104    1500    1005     595     438     250
 
SapI GCTCTTCn'nnn_

Cuts at:      0    7130    9366 
   Size:       7130    2236
 
ScaI AGT'ACT

Cuts at:      0    4513    9366 
   Size:       4513    4853
 
SexAI A'CCwGG_T

Cuts at:      0    2670    4212    9131    9366 
   Size:       2670    1542    4919     235

  Fragments arranged by size: 

                4919    2670    1542     235
 
SfcI C'TryA_G

Cuts at:      0    1410    1488    2159    3183    3378    3486    3604    5237 
   Size:       1410      78     671    1024     195     108     118    1633

Cuts at:   5237    5765    6587    6935    8255    8709    9366 
   Size:        528     822     348    1320     454     657

  Fragments arranged by size: 

                1633    1410    1320    1024     822     671     657     528
                 454     348     195     118     108      78
 
SmaI CCC'GGG

Cuts at:      0    5059    5284    9366 
   Size:       5059     225    4082

  Fragments arranged by size: 

                5059    4082     225
 
SmlI C'TyrA_G

Cuts at:      0    1893    3757    3815    3953    5114    6036    6355    7434 
   Size:       1893    1864      58     138    1161     922     319    1079

Cuts at:   7434    9217    9366 
   Size:       1783     149

  Fragments arranged by size: 

                1893    1864    1783    1161    1079     922     319     149
                 138      58
 
SphI G_CATG'C

Cuts at:      0    1031    4181    4693    9366 
   Size:       1031    3150     512    4673

  Fragments arranged by size: 

                4673    3150    1031     512
 
SspI AAT'ATT

Cuts at:      0     543    3937    5790    6507    7474    8028    9366 
   Size:        543    3394    1853     717     967     554    1338

  Fragments arranged by size: 

                3394    1853    1338     967     717     554     543
 
StuI AGG'CCT

Cuts at:      0      47    5125    7750    8094    9366 
   Size:         47    5078    2625     344    1272

  Fragments arranged by size: 

                5078    2625    1272     344      47
 
StyI C'CwwG_G

Cuts at:      0    1222    2218    3200    3620    7720    8078    9317    9366 
   Size:       1222     996     982     420    4100     358    1239      49

  Fragments arranged by size: 

                4100    1239    1222     996     982     420     358      49
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     819    1946    3150    3543    4948    8515    9366 
   Size:        819    1127    1204     393    1405    3567     851

  Fragments arranged by size: 

                3567    1405    1204    1127     851     819     393
 
TatI w'GTAC_w

Cuts at:      0     194    1112    1177    3268    4511    6150    9366 
   Size:        194     918      65    2091    1243    1639    3216

  Fragments arranged by size: 

                3216    2091    1639    1243     918     194      65
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     654     686    1983    2015    2420    2452    2970    3002 
   Size:        654      32    1297      32     405      32     518      32

Cuts at:   3002    4236    4268    4473    4505    6975    7007    8855    8887 
   Size:       1234      32     205      32    2470      32    1848      32

Cuts at:   8887    9366 
   Size:        479

  Fragments arranged by size: 

                2470    1848    1297    1234     654     518     479     405
                 205      32      32      32      32      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4621    6223    9366 
   Size:       4621    1602    3143

  Fragments arranged by size: 

                4621    3143    1602
 
XbaI T'CTAG_A

Cuts at:      0    5196    6810    9366 
   Size:       5196    1614    2556

  Fragments arranged by size: 

                5196    2556    1614
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1153    2797    3585    6184    9366 
   Size:       1153    1644     788    2599    3182

  Fragments arranged by size: 

                3182    2599    1644    1153     788
 
XhoI C'TCGA_G

Cuts at:      0    3953    5114    9366 
   Size:       3953    1161    4252

  Fragments arranged by size: 

                4252    3953    1161
 
XmaI C'CCGG_G

Cuts at:      0    5057    5282    9366 
   Size:       5057     225    4084

  Fragments arranged by size: 

                5057    4084     225
 
XmnI GAAnn'nnTTC

Cuts at:      0    1856    5147    5246    5920    6726    7110    7224    7270 
   Size:       1856    3291      99     674     806     384     114      46

Cuts at:   7270    8576    9366 
   Size:       1306     790

  Fragments arranged by size: 

                3291    1856    1306     806     790     674     384     114
                  99      46
 
ZraI GAC'GTC

Cuts at:      0    2304    6788    9366 
   Size:       2304    4484    2578

  Fragments arranged by size: 

                4484    2578    2304
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NotI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AscI    AsiSI     BaeI     BbeI     BmtI    BsiWI
    CspCI     EciI    EcoRV     FseI     FspI    FspAI    HaeII     KasI
     MluI     NarI     NheI     NruI     PacI     PmeI     PsiI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI
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