Restriction map for pF1KE2431
 (Circular) (Six-base) MAPSORT of: pF1KE2431.seq  Check: 5311  from: 1  to: 4040



pF1KE2431  4040 bp

 With 182 enzymes: SgfI * 

                        September 11, 2018 18:37  ..

AccI GT'mk_AC

Cuts at:   1078    1078 
   Size:       4040
 
Acc65I G'GTAC_C

Cuts at:   1056    1056 
   Size:       4040
 
AclI AA'CG_TT

Cuts at:   3880    3880 
   Size:       4040
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1865    2297    3044    3312    1865 
   Size:        432     747     268    2593

  Fragments arranged by size: 

                2593     747     432     268
 
AflIII A'CryG_T

Cuts at:   1392    2496    3473    1392 
   Size:       1104     977    1959

  Fragments arranged by size: 

                1959    1104     977
 
AgeI A'CCGG_T

Cuts at:   2428    2428 
   Size:       4040
 
AlwNI CAG_nnn'CTG

Cuts at:    395    1345    2912     395 
   Size:        950    1567    1523

  Fragments arranged by size: 

                1567    1523     950
 
ApaLI G'TGCA_C

Cuts at:   2810    2810 
   Size:       4040
 
ApoI r'AATT_y

Cuts at:    168     198     245     577    1044    3393    3649     168 
   Size:         30      47     332     467    2349     256     559

  Fragments arranged by size: 

                2349     559     467     332     256      47      30
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4040
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4040
 
AvaI C'yCGr_G

Cuts at:   1060    1060 
   Size:       4040
 
BamHI G'GATC_C

Cuts at:   1065    1065 
   Size:       4040
 
BanI G'GyrC_C

Cuts at:   1056    1470    1718    1753    1056 
   Size:        414     248      35    3343

  Fragments arranged by size: 

                3343     414     248      35
 
BanII G_rGCy'C

Cuts at:   1054    2084    1054 
   Size:       1030    3010
 
BbeI G_GCGC'C

Cuts at:   1474    1722    1474 
   Size:        248    3792
 
BbsI GAAGACnn'nnnn_

Cuts at:    879    1027     879 
   Size:        148    3892
 
BbvCI CC'TCA_GC

Cuts at:    412     412 
   Size:       4040
 
BciVI GTATCCnnnnn_n'

Cuts at:   1938    2699    4040    1938 
   Size:        761    1341    1938

  Fragments arranged by size: 

                1938    1341     761
 
BclI T'GATC_A

Cuts at:    377    1560     377 
   Size:       1183    2857
 
BglII A'GATC_T

Cuts at:    227    1555    3592     227 
   Size:       1328    2037     675

  Fragments arranged by size: 

                2037    1328     675
 
BlpI GC'TnA_GC

Cuts at:   1152    1152 
   Size:       4040
 
Bme1580I G_kGCm'C

Cuts at:   1665    1758    2814    1665 
   Size:         93    1056    2891

  Fragments arranged by size: 

                2891    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1014    1252    1333    1668    3812    1014 
   Size:        238      81     335    2144    1242

  Fragments arranged by size: 

                2144    1242     335     238      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2407    2407 
   Size:       4040
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    252    1016     252 
   Size:        764    3276
 
Bpu10I CC'TnA_GC

Cuts at:    412     412 
   Size:       4040
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    415     709     740    1216    2095    2587    2885     415 
   Size:        294      31     476     879     492     298    1570

  Fragments arranged by size: 

                1570     879     492     476     298     294      31
 
BsaI GGTCTCn'nnnn_

Cuts at:    270    3744     270 
   Size:       3474     566
 
BsaAI yAC'GTr

Cuts at:   1393    1393 
   Size:       4040
 
BsaBI GATnn'nnATC

Cuts at:      6    1554    3591       6 
   Size:       1548    2037     455

  Fragments arranged by size: 

                2037    1548     455
 
BsaHI Gr'CG_yC

Cuts at:   1471    1719    2421    3917    1471 
   Size:        248     702    1496    1594

  Fragments arranged by size: 

                1594    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1229    1750    2428    2702    2849    3260    1229 
   Size:        521     678     274     147     411    2009

  Fragments arranged by size: 

                2009     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1014    1044    1014 
   Size:         30    4010
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    489     489 
   Size:       4040
 
BseYI C'CCAG_C

Cuts at:    763    1465    2223    2800    3296     763 
   Size:        702     758     577     496    1507

  Fragments arranged by size: 

                1507     758     702     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1582    1628    2836     104 
   Size:       1478      46    1208    1308

  Fragments arranged by size: 

                1478    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1054    1832    2022    2814    1054 
   Size:        778     190     792    2280

  Fragments arranged by size: 

                2280     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1054    1665    1758    1832    2022    2084    2814    1054 
   Size:        611      93      74     190      62     730    2280

  Fragments arranged by size: 

                2280     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3260    3260 
   Size:       4040
 
BspHI T'CATG_A

Cuts at:   3216    3216 
   Size:       4040
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1090    1304    1606    1987    1090 
   Size:        214     302     381    3143

  Fragments arranged by size: 

                3143     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2332    2386    3870    4035      50 
   Size:       2282      54    1484     165      55

  Fragments arranged by size: 

                2282    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    626    1952     626 
   Size:       1326    2714
 
BsrFI r'CCGG_y

Cuts at:   2038    2219    2428    2038 
   Size:        181     209    3650

  Fragments arranged by size: 

                3650     209     181
 
BssSI C'ACGA_G

Cuts at:   2311    2669    2311 
   Size:        358    3682
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1417    1417 
   Size:       4040
 
BstBI TT'CG_AA

Cuts at:   2401    2401 
   Size:       4040
 
BstYI r'GATC_y

Cuts at:      1     227     751     759    1065    1532    1555    1890    2136 
   Size:        226     524       8     306     467      23     335     246

Cuts at:   2136    3148    3234    3246    3592       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     524     467     449     346     335     306     246
                 226      86      23      12       8
 
BtgI C'CryG_G

Cuts at:    647     741     647 
   Size:         94    3946
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1990    2171    2334    1990 
   Size:        181     163    3696

  Fragments arranged by size: 

                3696     181     163
 
BtsI GCAGTG_nn'

Cuts at:    251     380    1283     251 
   Size:        129     903    3008

  Fragments arranged by size: 

                3008     903     129
 
DraI TTT'AAA

Cuts at:   1039    1039 
   Size:       4040
 
DrdI GACnn_nn'nnGTC

Cuts at:   1746    2604    1746 
   Size:        858    3182
 
EaeI y'GGCC_r

Cuts at:   1625    1799    2190    2217    3562    1625 
   Size:        174     391      27    1345    2103

  Fragments arranged by size: 

                2103    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1625    1625 
   Size:       4040
 
EarI CTCTTCn'nnn_

Cuts at:    607    2063    2273     607 
   Size:       1456     210    2374

  Fragments arranged by size: 

                2374    1456     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2248    2558    2704    3850    2248 
   Size:        310     146    1146    2438

  Fragments arranged by size: 

                2438    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1052    1052 
   Size:       4040
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    252    1016    1865    2297    3044    3312     252 
   Size:        764     849     432     747     268     980

  Fragments arranged by size: 

                 980     849     764     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    511    1179     511 
   Size:        668    3372
 
EcoRI G'AATT_C

Cuts at:   1044    1044 
   Size:       4040
 
EcoRV GAT'ATC

Cuts at:    848     848 
   Size:       4040
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    249    2059     249 
   Size:       1810    2230
 
FspI TGC'GCA

Cuts at:   1821    1821 
   Size:       4040
 
HaeII r_GCGC'y

Cuts at:   1474    1722    2744    3723    1474 
   Size:        248    1022     979    1791

  Fragments arranged by size: 

                1791    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    212     244    1014    1046    1882    1912    1914    1944     212 
   Size:         32     770      32     836      30       2      30    2308

  Fragments arranged by size: 

                2308     836     770      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1079    1079 
   Size:       4040
 
HindIII A'AGCT_T

Cuts at:    785     785 
   Size:       4040
 
KasI G'GCGC_C

Cuts at:   1470    1718    1470 
   Size:        248    3792
 
KpnI G_GTAC'C

Cuts at:   1060    1060 
   Size:       4040
 
MluI A'CGCG_T

Cuts at:   3473    3473 
   Size:       4040
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    473    2711    2895     473 
   Size:       2238     184    1618

  Fragments arranged by size: 

                2238    1618     184
 
MscI TGG'CCA

Cuts at:   1801    1801 
   Size:       4040
 
MslI CAynn'nnrTG

Cuts at:    496    2029    2156     496 
   Size:       1533     127    2380

  Fragments arranged by size: 

                2380    1533     127
 
MspA1I CmG'CkG

Cuts at:   1151    1465    1825    2838    3083    1151 
   Size:        314     360    1013     245    2108

  Fragments arranged by size: 

                2108    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2221    2221 
   Size:       4040
 
NarI GG'CG_CC

Cuts at:   1471    1719    1471 
   Size:        248    3792
 
NcoI C'CATG_G

Cuts at:    647     647 
   Size:       4040
 
NgoMIV G'CCGG_C

Cuts at:   2219    2219 
   Size:       4040
 
NspI r_CATG'y

Cuts at:   1093    1593    2500    1093 
   Size:        500     907    2633

  Fragments arranged by size: 

                2633     907     500
 
PciI A'CATG_T

Cuts at:   2496    2496 
   Size:       4040
 
PmeI GTTT'AAAC

Cuts at:   1039    1039 
   Size:       4040
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3179    3211    3179 
   Size:         32    4008
 
PsiI TTA'TAA

Cuts at:    362     362 
   Size:       4040
 
PstI C_TGCA'G

Cuts at:   1087    1772    1087 
   Size:        685    3355
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4040
 
PvuII CAG'CTG

Cuts at:   1465    1825    1465 
   Size:        360    3680
 
RsrII CG'GwC_CG

Cuts at:   2235    2235 
   Size:       4040
 
SacI G_AGCT'C

Cuts at:   1054    1054 
   Size:       4040
 
SalI G'TCGA_C

Cuts at:   1077    1077 
   Size:       4040
 
SapI GCTCTTCn'nnn_

Cuts at:   2063    2273    2063 
   Size:        210    3830
 
SbfI CC_TGCA'GG

Cuts at:   1087    1087 
   Size:       4040
 
ScaI AGT'ACT

Cuts at:   3557    3557 
   Size:       4040
 
SfcI C'TryA_G

Cuts at:     33    1083    1768    2761    2952      33 
   Size:       1050     685     993     191    1121

  Fragments arranged by size: 

                1121    1050     993     685     191
 
SfoI GGC'GCC

Cuts at:   1472    1720    1472 
   Size:        248    3792
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4040
 
SmaI CCC'GGG

Cuts at:   1062    1062 
   Size:       4040
 
SmlI C'TyrA_G

Cuts at:    430     724     755    1195    2110    2602    2864     430 
   Size:        294      31     440     915     492     262    1606

  Fragments arranged by size: 

                1606     915     492     440     294     262      31
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4040
 
SphI G_CATG'C

Cuts at:   1093    1593    1093 
   Size:        500    3540
 
StyI C'CwwG_G

Cuts at:    647    1174    1528     647 
   Size:        527     354    3159

  Fragments arranged by size: 

                3159     527     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    947     996    1623    1756    2215    2424     947 
   Size:         49     627     133     459     209    2563

  Fragments arranged by size: 

                2563     627     459     209     133      49
 
TatI w'GTAC_w

Cuts at:    623    3555     623 
   Size:       2932    1108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3557    3589    3557 
   Size:         32    4008
 
Tth111I GACn'n_nGTC

Cuts at:   1837    1837 
   Size:       4040
 
XbaI T'CTAG_A

Cuts at:   1071    1071 
   Size:       4040
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    144    1535     144 
   Size:       1391    2649
 
XmaI C'CCGG_G

Cuts at:   1060    1060 
   Size:       4040
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI     PmeI     PpiI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI     FseI
    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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