Restriction map for pF1KE9645
 (Linear) (Six-base) MAPSORT of: KIEE9645.seq  Check: 3772  from: 1  to: 7476



KIEE9645.orf

 With 182 enzymes: SgfI * 

                        May 29, 2012 11:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    5593    6570    7476 
   Size:       5593     977     906

  Fragments arranged by size: 

                5593     977     906
 
AccI GT'mk_AC

Cuts at:      0    7125    7476 
   Size:       7125     351
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     185     203    1550    2231    2570    2651    2954 
   Size:        107      78      18    1347     681     339      81     303

Cuts at:   2954    2969    2987    3212    3506    3716    3857    4004    6317 
   Size:         15      18     225     294     210     141     147    2313

Cuts at:   6317    6706    6821    7476 
   Size:        389     115     655

  Fragments arranged by size: 

                2313    1347     681     655     389     339     303     294
                 225     210     147     141     115     107      81      78
                  18      18      15
 
AflII C'TTAA_G

Cuts at:      0    1174    4489    7476 
   Size:       1174    3315    2987

  Fragments arranged by size: 

                3315    2987    1174
 
AflIII A'CryG_T

Cuts at:      0    1971    2857    7476 
   Size:       1971     886    4619

  Fragments arranged by size: 

                4619    1971     886
 
AgeI A'CCGG_T

Cuts at:      0    1465    7476 
   Size:       1465    6011
 
AhdI GACnn_n'nnGTC

Cuts at:      0     529    7476 
   Size:        529    6947
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2759    7476 
   Size:       2759    4717
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2170    2191    2254    3763    3982    5137    5743    6025 
   Size:       2170      21      63    1509     219    1155     606     282

Cuts at:   6025    6552    7390    7476 
   Size:        527     838      86

  Fragments arranged by size: 

                2170    1509    1155     838     606     527     282     219
                  86      63      21
 
ApaLI G'TGCA_C

Cuts at:      0    7421    7476 
   Size:       7421      55
 
ApoI r'AATT_y

Cuts at:      0     330     433     855    1114    1239    2181    2267    2519 
   Size:        330     103     422     259     125     942      86     252

Cuts at:   2519    2598    2899    3071    3414    3639    3693    4436    4909 
   Size:         79     301     172     343     225      54     743     473

Cuts at:   4909    5205    5301    5473    5725    5874    6074    6301    7326 
   Size:        296      96     172     252     149     200     227    1025

Cuts at:   7326    7476 
   Size:        150

  Fragments arranged by size: 

                1025     942     743     473     422     343     330     301
                 296     259     252     252     227     225     200     172
                 172     150     149     125     103      96      86      79
                  54
 
AseI AT'TA_AT

Cuts at:      0    2261    6371    6983    7476 
   Size:       2261    4110     612     493

  Fragments arranged by size: 

                4110    2261     612     493
 
AvaI C'yCGr_G

Cuts at:      0    5885    7476 
   Size:       5885    1591
 
BamHI G'GATC_C

Cuts at:      0    5311    7476 
   Size:       5311    2165
 
BanII G_rGCy'C

Cuts at:      0      14    2401    6719    6945    7476 
   Size:         14    2387    4318     226     531

  Fragments arranged by size: 

                4318    2387     531     226      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     209     413     961    1238    1583    2432    2611    3698 
   Size:        209     204     548     277     345     849     179    1087

Cuts at:   3698    5717    6187    7476 
   Size:       2019     470    1289

  Fragments arranged by size: 

                2019    1289    1087     849     548     470     345     277
                 209     204     179
 
BbvCI CC'TCA_GC

Cuts at:      0    3473    6032    7476 
   Size:       3473    2559    1444

  Fragments arranged by size: 

                3473    2559    1444
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    6672    6693    6742    7476 
   Size:       6672      21      49     734

  Fragments arranged by size: 

                6672     734      49      21
 
BclI T'GATC_A

Cuts at:      0    1626    3633    5036    6570    7331    7476 
   Size:       1626    2007    1403    1534     761     145

  Fragments arranged by size: 

                2007    1626    1534    1403     761     145
 
BfrBI ATG'CAT

Cuts at:      0     728    1422    4769    7476 
   Size:        728     694    3347    2707

  Fragments arranged by size: 

                3347    2707     728     694
 
BglII A'GATC_T

Cuts at:      0    5881    7476 
   Size:       5881    1595
 
BlpI GC'TnA_GC

Cuts at:      0       8    7476 
   Size:          8    7468
 
Bme1580I G_kGCm'C

Cuts at:      0    1046    2594    7425    7476 
   Size:       1046    1548    4831      51

  Fragments arranged by size: 

                4831    1548    1046      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2424    6887    7306    7476 
   Size:       2424    4463     419     170

  Fragments arranged by size: 

                4463    2424     419     170
 
BmtI G_CTAG'C

Cuts at:      0    2206    7476 
   Size:       2206    5270
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2679    6698    7476 
   Size:       2679    4019     778

  Fragments arranged by size: 

                4019    2679     778
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1086    4076    4739    5978    6991    7225    7476 
   Size:       1086    2990     663    1239    1013     234     251

  Fragments arranged by size: 

                2990    1239    1086    1013     663     251     234
 
Bpu10I CC'TnA_GC

Cuts at:      0    2132    3059    3473    6032    6500    7476 
   Size:       2132     927     414    2559     468     976

  Fragments arranged by size: 

                2559    2132     976     927     468     414
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1615    2010    4972    5054    5811    7069    7476 
   Size:       1615     395    2962      82     757    1258     407

  Fragments arranged by size: 

                2962    1615    1258     757     407     395      82
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1075    7476 
   Size:       1075    6401
 
BsaAI yAC'GTr

Cuts at:      0    1972    7476 
   Size:       1972    5504
 
BsaBI GATnn'nnATC

Cuts at:      0    3632    7201    7476 
   Size:       3632    3569     275

  Fragments arranged by size: 

                3632    3569     275
 
BsaWI w'CCGG_w

Cuts at:      0    1465    7476 
   Size:       1465    6011
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      84     114    1084    1114    7476 
   Size:         84      30     970      30    6362

  Fragments arranged by size: 

                6362     970      84      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      92    1727    2797    4351    4366    4369    4378    4381 
   Size:         92    1635    1070    1554      15       3       9       3

Cuts at:   4381    4384    4387    4396    4399    5238    7476 
   Size:          3       3       9       3     839    2238

  Fragments arranged by size: 

                2238    1635    1554    1070     839      92      15       9
                   9       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    5988    7476 
   Size:       5988    1488
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     495    4669    5387    6257    7476 
   Size:         64     431    4174     718     870    1219

  Fragments arranged by size: 

                4174    1219     870     718     431      64
 
BsiEI CG_ry'CG

Cuts at:      0    1999    7476 
   Size:       1999    5477
 
BsiHKAI G_wGCw'C

Cuts at:      0    1418    2401    5412    6719    7425    7476 
   Size:       1418     983    3011    1307     706      51

  Fragments arranged by size: 

                3011    1418    1307     983     706      51
 
BsmI GAATG_Cn'

Cuts at:      0     728    4769    6045    7476 
   Size:        728    4041    1276    1431

  Fragments arranged by size: 

                4041    1431    1276     728
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4500    7476 
   Size:       4500    2976
 
Bsp1286I G_dGCh'C

Cuts at:      0      14    1046    1418    2401    2594    5412    6719    6945 
   Size:         14    1032     372     983     193    2818    1307     226

Cuts at:   6945    7425    7476 
   Size:        480      51

  Fragments arranged by size: 

                2818    1307    1032     983     480     372     226     193
                  51      14
 
BspHI T'CATG_A

Cuts at:      0      54    7307    7334    7476 
   Size:         54    7253      27     142

  Fragments arranged by size: 

                7253     142      54      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1524    4641    5593    6570    7476 
   Size:       1524    3117     952     977     906

  Fragments arranged by size: 

                3117    1524     977     952     906
 
BsrDI GCAATG_nn'

Cuts at:      0     122     571    6959    7476 
   Size:        122     449    6388     517

  Fragments arranged by size: 

                6388     517     449     122
 
BsrFI r'CCGG_y

Cuts at:      0    1465    7476 
   Size:       1465    6011
 
BsrGI T'GTAC_A

Cuts at:      0     381    7476 
   Size:        381    7095
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     360     725    7476 
   Size:        360     365    6751

  Fragments arranged by size: 

                6751     365     360
 
BstBI TT'CG_AA

Cuts at:      0    1959    6081    6234    7476 
   Size:       1959    4122     153    1242

  Fragments arranged by size: 

                4122    1959    1242     153
 
BstEII G'GTnAC_C

Cuts at:      0    7300    7476 
   Size:       7300     176
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     768    7476 
   Size:        768    6708
 
BstYI r'GATC_y

Cuts at:      0    1963    3868    5311    5881    6358    7476 
   Size:       1963    1905    1443     570     477    1118

  Fragments arranged by size: 

                1963    1905    1443    1118     570     477
 
Bsu36I CC'TnA_GG

Cuts at:      0    3086    4985    7476 
   Size:       3086    1899    2491

  Fragments arranged by size: 

                3086    2491    1899
 
BtgI C'CryG_G

Cuts at:      0    5378    6509    7476 
   Size:       5378    1131     967

  Fragments arranged by size: 

                5378    1131     967
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1270    2936    3677    7476 
   Size:       1270    1666     741    3799

  Fragments arranged by size: 

                3799    1666    1270     741
 
BtsI GCAGTG_nn'

Cuts at:      0     512     830    1653    4131    5847    7037    7476 
   Size:        512     318     823    2478    1716    1190     439

  Fragments arranged by size: 

                2478    1716    1190     823     512     439     318
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     643     678    7476 
   Size:        643      35    6798

  Fragments arranged by size: 

                6798     643      35
 
DraI TTT'AAA

Cuts at:      0     423    1121    2300    5942    7476 
   Size:        423     698    1179    3642    1534

  Fragments arranged by size: 

                3642    1534    1179     698     423
 
DraIII CAC_nnn'GTG

Cuts at:      0    4589    7476 
   Size:       4589    2887
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     204    2550    2670    3231    3301    3375    4328 
   Size:        179      25    2346     120     561      70      74     953

Cuts at:   4328    4343    4355    4373    4490    4496    5080    5586    6667 
   Size:         15      12      18     117       6     584     506    1081

Cuts at:   6667    6785    6987    7152    7476 
   Size:        118     202     165     324

  Fragments arranged by size: 

                2346    1081     953     584     561     506     324     202
                 179     165     120     118     117      74      70      25
                  18      15      12       6
 
EcoICRI GAG'CTC

Cuts at:      0    2399    6717    7476 
   Size:       2399    4318     759

  Fragments arranged by size: 

                4318    2399     759
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     185     203    1086    1550    2231    2570    2651 
   Size:        107      78      18     883     464     681     339      81

Cuts at:   2651    2954    2969    2987    3212    3506    3716    3857    4004 
   Size:        303      15      18     225     294     210     141     147

Cuts at:   4004    4076    4739    5978    6317    6706    6821    6991    7225 
   Size:         72     663    1239     339     389     115     170     234

Cuts at:   7225    7476 
   Size:        251

  Fragments arranged by size: 

                1239     883     681     663     464     389     339     339
                 303     294     251     234     225     210     170     147
                 141     115     107      81      78      72      18      18
                  15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2215    4421    7460    7476 
   Size:       2215    2206    3039      16

  Fragments arranged by size: 

                3039    2215    2206      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     427    5134    6975    7453    7476 
   Size:        427    4707    1841     478      23

  Fragments arranged by size: 

                4707    1841     478     427      23
 
EcoRI G'AATT_C

Cuts at:      0    2519    3639    5205    5874    7476 
   Size:       2519    1120    1566     669    1602

  Fragments arranged by size: 

                2519    1602    1566    1120     669
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     508     615    1405    3865    6175    7476 
   Size:        508     107     790    2460    2310    1301

  Fragments arranged by size: 

                2460    2310    1301     790     508     107
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     515     547    1537    1569    1660    1692    2160    2192 
   Size:        515      32     990      32      91      32     468      32

Cuts at:   2192    2527    2559    2826    2858    2866    2898    3163    3195 
   Size:        335      32     267      32       8      32     265      32

Cuts at:   3195    3307    3339    3586    3618    3853    3885    4036    4068 
   Size:        112      32     247      32     235      32     151      32

Cuts at:   4068    4396    4428    5737    5769    5809    5841    6323    6355 
   Size:        328      32    1309      32      40      32     482      32

Cuts at:   6355    6513    6545    7029    7061    7151    7183    7323    7355 
   Size:        158      32     484      32      90      32     140      32

Cuts at:   7355    7476 
   Size:        121

  Fragments arranged by size: 

                1309     990     515     484     482     468     335     328
                 267     265     247     235     158     151     140     121
                 112      91      90      40      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                   8
 
HincII GTy'rAC

Cuts at:      0     900    3200    6797    7476 
   Size:        900    2300    3597     679

  Fragments arranged by size: 

                3597    2300     900     679
 
HindIII A'AGCT_T

Cuts at:      0      49    2767    4948    5117    5611    7476 
   Size:         49    2718    2181     169     494    1865

  Fragments arranged by size: 

                2718    2181    1865     494     169      49
 
MfeI C'AATT_G

Cuts at:      0     251    7476 
   Size:        251    7225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     800    1459    4214    5071    7476 
   Size:        800     659    2755     857    2405

  Fragments arranged by size: 

                2755    2405     857     800     659
 
MslI CAynn'nnrTG

Cuts at:      0     766    1427    5495    6758    7476 
   Size:        766     661    4068    1263     718

  Fragments arranged by size: 

                4068    1263     766     718     661
 
MspA1I CmG'CkG

Cuts at:      0       7    4200    4248    6969    7066    7208    7476 
   Size:          7    4193      48    2721      97     142     268

  Fragments arranged by size: 

                4193    2721     268     142      97      48       7
 
NcoI C'CATG_G

Cuts at:      0    5378    6509    7476 
   Size:       5378    1131     967

  Fragments arranged by size: 

                5378    1131     967
 
NdeI CA'TA_TG

Cuts at:      0    3258    7476 
   Size:       3258    4218
 
NheI G'CTAG_C

Cuts at:      0    2202    7476 
   Size:       2202    5274
 
NsiI A_TGCA'T

Cuts at:      0     730    1424    4771    7476 
   Size:        730     694    3347    2705

  Fragments arranged by size: 

                3347    2705     730     694
 
NspI r_CATG'y

Cuts at:      0     839    1422    2861    5004    7476 
   Size:        839     583    1439    2143    2472

  Fragments arranged by size: 

                2472    2143    1439     839     583
 
PciI A'CATG_T

Cuts at:      0    2857    7476 
   Size:       2857    4619
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4785    7390    7476 
   Size:       4785    2605      86

  Fragments arranged by size: 

                4785    2605      86
 
PfoI T'CCnGG_A

Cuts at:      0    4416    7449    7476 
   Size:       4416    3033      27

  Fragments arranged by size: 

                4416    3033      27
 
PmlI CAC'GTG

Cuts at:      0    1972    7476 
   Size:       1972    5504
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2223    2255    7476 
   Size:       2223      32    5221

  Fragments arranged by size: 

                5221    2223      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    6975    7453    7476 
   Size:       6975     478      23

  Fragments arranged by size: 

                6975     478      23
 
PsiI TTA'TAA

Cuts at:      0    5341    6212    7476 
   Size:       5341     871    1264

  Fragments arranged by size: 

                5341    1264     871
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4685    4717    7476 
   Size:       4685      32    2759

  Fragments arranged by size: 

                4685    2759      32
 
PstI C_TGCA'G

Cuts at:      0    5009    7476 
   Size:       5009    2467
 
PvuII CAG'CTG

Cuts at:      0    6969    7066    7208    7476 
   Size:       6969      97     142     268

  Fragments arranged by size: 

                6969     268     142      97
 
SacI G_AGCT'C

Cuts at:      0    2401    6719    7476 
   Size:       2401    4318     757

  Fragments arranged by size: 

                4318    2401     757
 
SapI GCTCTTCn'nnn_

Cuts at:      0     204    7476 
   Size:        204    7272
 
ScaI AGT'ACT

Cuts at:      0    5545    7476 
   Size:       5545    1931
 
SfcI C'TryA_G

Cuts at:      0    1733    2120    3644    5005    7476 
   Size:       1733     387    1524    1361    2471

  Fragments arranged by size: 

                2471    1733    1524    1361     387
 
SmlI C'TyrA_G

Cuts at:      0    1174    1594    1989    4489    4951    5069    5826    7048 
   Size:       1174     420     395    2500     462     118     757    1222

Cuts at:   7048    7476 
   Size:        428

  Fragments arranged by size: 

                2500    1222    1174     757     462     428     420     395
                 118
 
SpeI A'CTAG_T

Cuts at:      0     958    7476 
   Size:        958    6518
 
SspI AAT'ATT

Cuts at:      0    1692    3343    5108    7476 
   Size:       1692    1651    1765    2368

  Fragments arranged by size: 

                2368    1765    1692    1651
 
StyI C'CwwG_G

Cuts at:      0     741    4791    5378    5656    6509    7461    7476 
   Size:        741    4050     587     278     853     952      15

  Fragments arranged by size: 

                4050     952     853     741     587     278      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     821    2013    4596    7476 
   Size:        821    1192    2583    2880

  Fragments arranged by size: 

                2880    2583    1192     821
 
TatI w'GTAC_w

Cuts at:      0     381    2868    5543    7104    7250    7476 
   Size:        381    2487    2675    1561     146     226

  Fragments arranged by size: 

                2675    2487    1561     381     226     146
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4575    4607    5066    5098    7476 
   Size:       4575      32     459      32    2378

  Fragments arranged by size: 

                4575    2378     459      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     826    7476 
   Size:        826    6650
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4693    5996    7476 
   Size:       4693    1303    1480

  Fragments arranged by size: 

                4693    1480    1303
 
XmnI GAAnn'nnTTC

Cuts at:      0    2346    3297    5517    6077    6155    7476 
   Size:       2346     951    2220     560      78    1321

  Fragments arranged by size: 

                2346    2220    1321     951     560      78
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AgeI     AhdI     AlfI
    AlwNI    ApaLI     ApoI     AseI     AvaI    BamHI    BanII     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MslI
   MspA1I     NcoI     NdeI     NheI     NsiI     NspI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI     PsiI     PsrI     PstI    PvuII
     SacI     SapI     ScaI     SfcI     SmlI     SpeI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AleI     AloI     ApaI     AscI
    AsiSI    AvrII     BaeI     BanI     BbeI     BcgI     BglI    BmgBI
    BsaHI    BsiWI    BspEI    BsrBI   BssHII    BssSI  BstZ17I     ClaI
     DrdI     EaeI     EagI     EciI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI
   NgoMIV     NotI     NruI     PacI     PasI     PmeI    PshAI   PspOMI
    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI     XbaI     XhoI     XmaI     ZraI
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