Restriction map for
pF1KE9669
(Circular) (Six-base) MAPSORT of: pF1KE9669.seq Check: 4089 from: 1 to: 12104
pF1KE9669 12104 bp
With 182 enzymes: SgfI *
October 13, 2011 18:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3618 3618
Size: 12104
AatII G_ACGT'C
Cuts at: 3675 7705 3675
Size: 4030 8074
AccI GT'mk_AC
Cuts at: 482 9142 482
Size: 8660 3444
Acc65I G'GTAC_C
Cuts at: 561 4097 9120 561
Size: 3536 5023 3545
Fragments arranged by size:
5023 3545 3536
AclI AA'CG_TT
Cuts at: 11944 11944
Size: 12104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2408 3457 3525 3563 3911 4033 4877 5320
Size: 69 1049 68 38 348 122 844 443
Cuts at: 5320 5465 5717 6135 6974 7912 8365 8872 9929
Size: 145 252 418 839 938 453 507 1057
Cuts at: 9929 10361 11108 11376 2339
Size: 432 747 268 3067
Fragments arranged by size:
3067 1057 1049 938 844 839 747 507
453 443 432 418 348 268 252 145
122 69 68 38
AfeI AGC'GCT
Cuts at: 2380 2380
Size: 12104
AflII C'TTAA_G
Cuts at: 6340 6340
Size: 12104
AflIII A'CryG_T
Cuts at: 5839 8531 9456 10560 11537 5839
Size: 2692 925 1104 977 6406
Fragments arranged by size:
6406 2692 1104 977 925
AgeI A'CCGG_T
Cuts at: 10492 10492
Size: 12104
AhdI GACnn_n'nnGTC
Cuts at: 3377 5840 6789 6855 3377
Size: 2463 949 66 8626
Fragments arranged by size:
8626 2463 949 66
AleI CACnn'nnGTG
Cuts at: 1214 7165 1214
Size: 5951 6153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4000 6301 4000
Size: 2301 9803
AlwNI CAG_nnn'CTG
Cuts at: 802 2437 3449 5170 6787 6889 7531 8695 9409
Size: 1635 1012 1721 1617 102 642 1164 714
Cuts at: 9409 10976 802
Size: 1567 1930
Fragments arranged by size:
1930 1721 1635 1617 1567 1164 1012 714
642 102
ApaI G_GGCC'C
Cuts at: 547 638 1462 547
Size: 91 824 11189
Fragments arranged by size:
11189 824 91
ApaLI G'TGCA_C
Cuts at: 4517 10874 4517
Size: 6357 5747
ApoI r'AATT_y
Cuts at: 1098 2277 2581 2660 3208 3601 3780 3899 4359
Size: 1179 304 79 548 393 179 119 460
Cuts at: 4359 4549 4692 5647 5716 5724 6906 7476 8140
Size: 190 143 955 69 8 1182 570 664
Cuts at: 8140 9108 11457 11713 1098
Size: 968 2349 256 1489
Fragments arranged by size:
2349 1489 1182 1179 968 955 664 570
548 460 393 304 256 190 179 143
119 79 69 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 12104
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12104
AvaI C'yCGr_G
Cuts at: 1668 5135 5939 6956 8805 9124 1668
Size: 3467 804 1017 1849 319 4648
Fragments arranged by size:
4648 3467 1849 1017 804 319
BamHI G'GATC_C
Cuts at: 1935 5423 7811 9129 1935
Size: 3488 2388 1318 4910
Fragments arranged by size:
4910 3488 2388 1318
BanI G'GyrC_C
Cuts at: 444 561 843 1429 1506 4097 7115 8046 8094
Size: 117 282 586 77 2591 3018 931 48
Cuts at: 8094 9120 9534 9782 9817 444
Size: 1026 414 248 35 2731
Fragments arranged by size:
3018 2731 2591 1026 931 586 414 282
248 117 77 48 35
BanII G_rGCy'C
Cuts at: 547 582 638 1462 1790 2102 5958 7205 8905
Size: 35 56 824 328 312 3856 1247 1700
Cuts at: 8905 9118 10148 547
Size: 213 1030 2503
Fragments arranged by size:
3856 2503 1700 1247 1030 824 328 312
213 56 35
BbeI G_GCGC'C
Cuts at: 7119 8098 9538 9786 7119
Size: 979 1440 248 9437
Fragments arranged by size:
9437 1440 979 248
BbsI GAAGACnn'nnnn_
Cuts at: 1679 2396 2855 4082 5672 5920 6860 8624 8666
Size: 717 459 1227 1590 248 940 1764 42
Cuts at: 8666 9029 1679
Size: 363 4754
Fragments arranged by size:
4754 1764 1590 1227 940 717 459 363
248 42
BbvCI CC'TCA_GC
Cuts at: 656 1406 7724 8933 656
Size: 750 6318 1209 3827
Fragments arranged by size:
6318 3827 1209 750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1021 1055 1462 1496 7330 7364 7556 7590 1021
Size: 34 407 34 5834 34 192 34 5535
Fragments arranged by size:
5834 5535 407 192 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1358 6051 10002 10763 12104 1358
Size: 4693 3951 761 1341 1358
Fragments arranged by size:
4693 3951 1358 1341 761
BclI T'GATC_A
Cuts at: 315 4569 5108 9624 315
Size: 4254 539 4516 2795
Fragments arranged by size:
4516 4254 2795 539
BfrBI ATG'CAT
Cuts at: 348 1251 348
Size: 903 11201
BglI GCCn_nnn'nGGC
Cuts at: 761 8426 761
Size: 7665 4439
BglII A'GATC_T
Cuts at: 2640 5384 9619 11656 2640
Size: 2744 4235 2037 3088
Fragments arranged by size:
4235 3088 2744 2037
BlpI GC'TnA_GC
Cuts at: 1841 6998 7199 9216 1841
Size: 5157 201 2017 4729
Fragments arranged by size:
5157 4729 2017 201
Bme1580I G_kGCm'C
Cuts at: 449 547 638 824 1462 1529 1722 4521 9729
Size: 98 91 186 638 67 193 2799 5208
Cuts at: 9729 9822 10878 449
Size: 93 1056 1675
Fragments arranged by size:
5208 2799 1675 1056 638 193 186 98
93 91 67
BmgBI CAC'GTC
Cuts at: 5842 8321 5842
Size: 2479 9625
BmrI ACTGGGnnnn_n'
Cuts at: 1208 1532 1934 2463 2500 3207 4411 6797 8231
Size: 324 402 529 37 707 1204 2386 1434
Cuts at: 8231 8854 9316 9397 9732 11876 1208
Size: 623 462 81 335 2144 1436
Fragments arranged by size:
2386 2144 1436 1434 1204 707 623 529
462 402 335 324 81 37
BmtI G_CTAG'C
Cuts at: 5626 8773 5626
Size: 3147 8957
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4153 6276 6759 9069 10471 4153
Size: 2123 483 2310 1402 5786
Fragments arranged by size:
5786 2310 2123 1402 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 2579 3502 3923 3937 4273 6554 6862 8138
Size: 163 923 421 14 336 2281 308 1276
Cuts at: 8138 8374 8383 8392 8420 8822 8889 2416
Size: 236 9 9 28 402 67 5631
Fragments arranged by size:
5631 2281 1276 923 421 402 336 308
236 163 67 28 14 9 9
Bpu10I CC'TnA_GC
Cuts at: 656 1406 3383 4295 4637 7724 8933 656
Size: 750 1977 912 342 3087 1209 3827
Fragments arranged by size:
3827 3087 1977 1209 912 750 342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3454 3793 5388 7434 9280 10159 10651 10949 3454
Size: 339 1595 2046 1846 879 492 298 4609
Fragments arranged by size:
4609 2046 1846 1595 879 492 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 1559 5363 7410 7750 8953 11808 1559
Size: 3804 2047 340 1203 2855 1855
Fragments arranged by size:
3804 2855 2047 1855 1203 340
BsaAI yAC'GTr
Cuts at: 691 819 2782 2858 9457 691
Size: 128 1963 76 6599 3338
Fragments arranged by size:
6599 3338 1963 128 76
BsaBI GATnn'nnATC
Cuts at: 6 1167 4568 9618 11655 6
Size: 1161 3401 5050 2037 455
Fragments arranged by size:
5050 3401 2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 3672 4884 7116 7702 8095 9535 9783 10485 11981
Size: 1212 2232 586 393 1440 248 702 1496
Cuts at: 11981 3672
Size: 3795
Fragments arranged by size:
3795 2232 1496 1440 1212 702 586 393
248
BsaWI w'CCGG_w
Cuts at: 2762 5155 8613 9293 9814 10492 10766 10913 11324
Size: 2393 3458 680 521 678 274 147 411
Cuts at: 11324 2762
Size: 3542
Fragments arranged by size:
3542 3458 2393 680 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 896 926 1320 1350 1635 1665 2552
Size: 30 349 30 394 30 285 30 887
Cuts at: 2552 2582 2792 2822 4147 4177 4908 4938 6211
Size: 30 210 30 1325 30 731 30 1273
Cuts at: 6211 6241 7239 7269 517
Size: 30 998 30 5352
Fragments arranged by size:
5352 1325 1273 998 887 731 394 349
285 210 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1322 1325 2300 2303 2431 2578 2911 4546 6216
Size: 3 975 3 128 147 333 1635 1670
Cuts at: 6216 6265 6557 6649 7203 7247 7323 7326 7329
Size: 49 292 92 554 44 76 3 3
Cuts at: 7329 7332 7653 8653 8998 1322
Size: 3 321 1000 345 4428
Fragments arranged by size:
4428 1670 1635 1000 975 554 345 333
321 292 147 128 92 76 49 44
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 824 835 1360 2440 6323 6327 6648 6872 7382
Size: 11 525 1080 3883 4 321 224 510
Cuts at: 7382 8871 9529 10287 10864 11360 824
Size: 1489 658 758 577 496 1568
Fragments arranged by size:
3883 1568 1489 1080 758 658 577 525
510 496 321 224 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 886 4826 5248 7733 7882 626
Size: 260 3940 422 2485 149 4848
Fragments arranged by size:
4848 3940 2485 422 260 149
BsiEI CG_ry'CG
Cuts at: 104 4122 8300 9646 9692 10900 104
Size: 4018 4178 1346 46 1208 1308
Fragments arranged by size:
4178 4018 1346 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4521 5215 5958 6940 7205 9118 9896 10086 10878
Size: 694 743 982 265 1913 778 190 792
Cuts at: 10878 4521
Size: 5747
Fragments arranged by size:
5747 1913 982 792 778 743 694 265
190
BsmI GAATG_Cn'
Cuts at: 1773 1842 6194 7947 8898 1773
Size: 69 4352 1753 951 4979
Fragments arranged by size:
4979 4352 1753 951 69
BsmBI CGTCTCn'nnnn_
Cuts at: 410 2343 3663 3679 7259 410
Size: 1933 1320 16 3580 5255
Fragments arranged by size:
5255 3580 1933 1320 16
Bsp1286I G_dGCh'C
Cuts at: 449 547 582 638 824 1462 1529 1722 1790
Size: 98 35 56 186 638 67 193 68
Cuts at: 1790 2102 4521 5215 5958 6940 7205 8905 9118
Size: 312 2419 694 743 982 265 1700 213
Cuts at: 9118 9729 9822 9896 10086 10148 10878 449
Size: 611 93 74 190 62 730 1675
Fragments arranged by size:
2419 1700 1675 982 743 730 694 638
611 312 265 213 193 190 186 98
93 74 68 67 62 56 35
BspEI T'CCGG_A
Cuts at: 11324 11324
Size: 12104
BspHI T'CATG_A
Cuts at: 7008 7685 11280 7008
Size: 677 3595 7832
Fragments arranged by size:
7832 3595 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1874 3618 4255 6297 6508 9154 9368 9670 10051
Size: 1744 637 2042 211 2646 214 302 381
Cuts at: 10051 1874
Size: 3927
Fragments arranged by size:
3927 2646 2042 1744 637 381 302 214
211
BsrBI CCG'CTC
Cuts at: 50 2865 10396 10450 11934 12099 50
Size: 2815 7531 54 1484 165 55
Fragments arranged by size:
7531 2815 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1316 3965 6930 10016 1316
Size: 2649 2965 3086 3404
Fragments arranged by size:
3404 3086 2965 2649
BsrFI r'CCGG_y
Cuts at: 621 5220 5849 5921 10102 10283 10492 621
Size: 4599 629 72 4181 181 209 2233
Fragments arranged by size:
4599 4181 2233 629 209 181 72
BsrGI T'GTAC_A
Cuts at: 4201 4201
Size: 12104
BssSI C'ACGA_G
Cuts at: 10375 10733 10375
Size: 358 11746
BstAPI GCAn_nnn'nTGC
Cuts at: 1758 2167 2428 2437 7720 8452 9481 1758
Size: 409 261 9 5283 732 1029 4381
Fragments arranged by size:
5283 4381 1029 732 409 261 9
BstBI TT'CG_AA
Cuts at: 10465 10465
Size: 12104
BstEII G'GTnAC_C
Cuts at: 190 7728 190
Size: 7538 4566
BstXI CCAn_nnnn'nTGG
Cuts at: 1216 1426 3973 4697 6377 7167 8124 1216
Size: 210 2547 724 1680 790 957 5196
Fragments arranged by size:
5196 2547 1680 957 790 724 210
BstYI r'GATC_y
Cuts at: 1 114 1935 2640 3300 4491 4574 5103 5384
Size: 113 1821 705 660 1191 83 529 281
Cuts at: 5384 5423 6296 6363 6984 7811 8109 8722 9129
Size: 39 873 67 621 827 298 613 407
Cuts at: 9129 9596 9619 9954 10200 11212 11298 11310 11656
Size: 467 23 335 246 1012 86 12 346
Cuts at: 11656 1
Size: 449
Fragments arranged by size:
1821 1191 1012 873 827 705 660 621
613 529 467 449 407 346 335 298
281 246 113 86 83 67 39 23
12
Bsu36I CC'TnA_GG
Cuts at: 440 795 1265 7040 440
Size: 355 470 5775 5504
Fragments arranged by size:
5775 5504 470 355
BtgI C'CryG_G
Cuts at: 107 450 900 1433 3153 3400 4879 4986 5753
Size: 343 450 533 1720 247 1479 107 767
Cuts at: 5753 7208 8294 8897 107
Size: 1455 1086 603 3314
Fragments arranged by size:
3314 1720 1479 1455 1086 767 603 533
450 343 247 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8483 8990 10054 10235 10398 8483
Size: 507 1064 181 163 10189
Fragments arranged by size:
10189 1064 507 181 163
BtsI GCAGTG_nn'
Cuts at: 465 1654 4232 4814 6938 7091 9347 465
Size: 1189 2578 582 2124 153 2256 3222
Fragments arranged by size:
3222 2578 2256 2124 1189 582 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3786 3821 3786
Size: 35 12069
DraI TTT'AAA
Cuts at: 3915 8949 9103 3915
Size: 5034 154 6916
Fragments arranged by size:
6916 5034 154
DraIII CAC_nnn'GTG
Cuts at: 864 3495 4032 9040 864
Size: 2631 537 5008 3928
Fragments arranged by size:
5008 3928 2631 537
DrdI GACnn_nn'nnGTC
Cuts at: 7994 9006 9810 10668 7994
Size: 1012 804 858 9430
Fragments arranged by size:
9430 1012 858 804
EaeI y'GGCC_r
Cuts at: 804 1599 4023 4119 5127 5246 6164 7102 7215
Size: 795 2424 96 1008 119 918 938 113
Cuts at: 7215 8297 9689 9863 10254 10281 11626 804
Size: 1082 1392 174 391 27 1345 1282
Fragments arranged by size:
2424 1392 1345 1282 1082 1008 938 918
795 391 174 119 113 96 27
EagI C'GGCC_G
Cuts at: 4119 8297 9689 4119
Size: 4178 1392 6534
Fragments arranged by size:
6534 4178 1392
EarI CTCTTCn'nnn_
Cuts at: 1846 2341 2365 3544 3617 3887 3902 4434 6006
Size: 495 24 1179 73 270 15 532 1572
Cuts at: 6006 6011 6674 6815 6824 6857 7180 7630 8250
Size: 5 663 141 9 33 323 450 620
Cuts at: 8250 8818 9038 10127 10337 1846
Size: 568 220 1089 210 3613
Fragments arranged by size:
3613 1572 1179 1089 663 620 568 532
495 450 323 270 220 210 141 73
33 24 15 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4129 5392 10312 10622 10768 11914 4129
Size: 1263 4920 310 146 1146 4319
Fragments arranged by size:
4920 4319 1263 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5956 7203 9116 5956
Size: 1247 1913 8944
Fragments arranged by size:
8944 1913 1247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2408 2416 2579 3457 3502 3525 3563 3911
Size: 69 8 163 878 45 23 38 348
Cuts at: 3911 3923 3937 4033 4273 4877 5320 5465 5717
Size: 12 14 96 240 604 443 145 252
Cuts at: 5717 6135 6554 6862 6974 7912 8138 8365 8374
Size: 418 419 308 112 938 226 227 9
Cuts at: 8374 8383 8392 8420 8822 8872 8889 9929 10361
Size: 9 9 28 402 50 17 1040 432
Cuts at: 10361 11108 11376 2339
Size: 747 268 3067
Fragments arranged by size:
3067 1040 938 878 747 604 443 432
419 418 402 348 308 268 252 240
227 226 163 145 112 96 69 50
45 38 28 23 17 14 12 9
9 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 769 1063 4141 4368 5272 6108 769
Size: 294 3078 227 904 836 6765
Fragments arranged by size:
6765 3078 904 836 294 227
EcoO109I rG'GnC_Cy
Cuts at: 543 544 634 635 762 799 1459 2015 2331
Size: 1 90 1 127 37 660 556 316
Cuts at: 2331 3326 4508 5319 5904 8941 9243 543
Size: 995 1182 811 585 3037 302 3404
Fragments arranged by size:
3404 3037 1182 995 811 660 585 556
316 302 127 90 37 1 1
EcoRI G'AATT_C
Cuts at: 2581 2660 3208 9108 2581
Size: 79 548 5900 5577
Fragments arranged by size:
5900 5577 548 79
EcoRV GAT'ATC
Cuts at: 1045 1045
Size: 12104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6944 7209 7521 7588 10123 6944
Size: 265 312 67 2535 8925
Fragments arranged by size:
8925 2535 312 265 67
FspI TGC'GCA
Cuts at: 9885 9885
Size: 12104
HaeII r_GCGC'y
Cuts at: 2382 3408 7119 8098 9538 9786 10808 11787 2382
Size: 1026 3711 979 1440 248 1022 979 2699
Fragments arranged by size:
3711 2699 1440 1026 1022 979 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 517 549 2440 2472 2802 2834 3363 3395 4147
Size: 32 1891 32 330 32 529 32 752
Cuts at: 4147 4179 4528 4560 5129 5161 5826 5858 6109
Size: 32 349 32 569 32 665 32 251
Cuts at: 6109 6141 6211 6243 6432 6464 6775 6807 6841
Size: 32 70 32 189 32 311 32 34
Cuts at: 6841 6873 7347 7379 9946 9976 9978 10008 517
Size: 32 474 32 2567 30 2 30 2613
Fragments arranged by size:
2613 2567 1891 752 665 569 529 474
349 330 311 251 189 70 34 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1077 1618 2250 2760 5148 9143 1077
Size: 541 632 510 2388 3995 4038
Fragments arranged by size:
4038 3995 2388 632 541 510
HindIII A'AGCT_T
Cuts at: 5091 6357 7736 5091
Size: 1266 1379 9459
Fragments arranged by size:
9459 1379 1266
HpaI GTT'AAC
Cuts at: 1077 1077
Size: 12104
KasI G'GCGC_C
Cuts at: 7115 8094 9534 9782 7115
Size: 979 1440 248 9437
Fragments arranged by size:
9437 1440 979 248
KpnI G_GTAC'C
Cuts at: 565 4101 9124 565
Size: 3536 5023 3545
Fragments arranged by size:
5023 3545 3536
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 12104
MluI A'CGCG_T
Cuts at: 11537 11537
Size: 12104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1763 2483 3005 3247 3440 6418 6531 6839 10775
Size: 720 522 242 193 2978 113 308 3936
Cuts at: 10775 10959 1763
Size: 184 2908
Fragments arranged by size:
3936 2978 2908 720 522 308 242 193
184 113
MscI TGG'CCA
Cuts at: 806 1601 4025 5129 5248 6166 7104 9865 806
Size: 795 2424 1104 119 918 938 2761 3045
Fragments arranged by size:
3045 2761 2424 1104 938 918 795 119
MslI CAynn'nnrTG
Cuts at: 1214 3592 3841 5047 5513 7165 7372 10093 10220
Size: 2378 249 1206 466 1652 207 2721 127
Cuts at: 10220 1214
Size: 3098
Fragments arranged by size:
3098 2721 2378 1652 1206 466 249 207
127
MspA1I CmG'CkG
Cuts at: 5122 5252 6327 6652 6704 7305 7794 8296 8305
Size: 130 1075 325 52 601 489 502 9
Cuts at: 8305 8412 8452 8572 9215 9529 9889 10902 11147
Size: 107 40 120 643 314 360 1013 245
Cuts at: 11147 5122
Size: 6079
Fragments arranged by size:
6079 1075 1013 643 601 502 489 360
325 314 245 130 120 107 52 40
9
NaeI GCC'GGC
Cuts at: 623 5851 10285 623
Size: 5228 4434 2442
Fragments arranged by size:
5228 4434 2442
NarI GG'CG_CC
Cuts at: 7116 8095 9535 9783 7116
Size: 979 1440 248 9437
Fragments arranged by size:
9437 1440 979 248
NcoI C'CATG_G
Cuts at: 107 450 900 1433 3153 3400 5753 7208 107
Size: 343 450 533 1720 247 2353 1455 5003
Fragments arranged by size:
5003 2353 1720 1455 533 450 343 247
NdeI CA'TA_TG
Cuts at: 4104 4104
Size: 12104
NgoMIV G'CCGG_C
Cuts at: 621 5849 10283 621
Size: 5228 4434 2442
Fragments arranged by size:
5228 4434 2442
NheI G'CTAG_C
Cuts at: 5622 8769 5622
Size: 3147 8957
NotI GC'GGCC_GC
Cuts at: 8297 8297
Size: 12104
NsiI A_TGCA'T
Cuts at: 350 1253 350
Size: 903 11201
NspI r_CATG'y
Cuts at: 495 666 1473 1500 3066 5340 5622 5767 9157
Size: 171 807 27 1566 2274 282 145 3390
Cuts at: 9157 9657 10564 495
Size: 500 907 2035
Fragments arranged by size:
3390 2274 2035 1566 907 807 500 282
171 145 27
PasI CC'CwG_GG
Cuts at: 388 548 3306 388
Size: 160 2758 9186
Fragments arranged by size:
9186 2758 160
PciI A'CATG_T
Cuts at: 10560 10560
Size: 12104
PfoI T'CCnGG_A
Cuts at: 117 1580 5273 7785 8436 8838 117
Size: 1463 3693 2512 651 402 3383
Fragments arranged by size:
3693 3383 2512 1463 651 402
PmeI GTTT'AAAC
Cuts at: 9103 9103
Size: 12104
PmlI CAC'GTG
Cuts at: 691 819 2858 691
Size: 128 2039 9937
Fragments arranged by size:
9937 2039 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2517 2549 8659 8691 11243 11275 2517
Size: 32 6110 32 2552 32 3346
Fragments arranged by size:
6110 3346 2552 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 799 2015 2331 3326 5319 8941 799
Size: 1216 316 995 1993 3622 3962
Fragments arranged by size:
3962 3622 1993 1216 995 316
PshAI GACnn'nnGTC
Cuts at: 402 402
Size: 12104
PspOMI G'GGCC_C
Cuts at: 543 634 1458 543
Size: 91 824 11189
Fragments arranged by size:
11189 824 91
PspXI vC'TCGA_Gb
Cuts at: 5135 5135
Size: 12104
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1577 1609 1577
Size: 32 12072
PstI C_TGCA'G
Cuts at: 1661 5038 5457 9006 9151 9836 1661
Size: 3377 419 3549 145 685 3929
Fragments arranged by size:
3929 3549 3377 685 419 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12104
PvuII CAG'CTG
Cuts at: 5252 6327 6652 7794 8412 8452 8572 9529 9889
Size: 1075 325 1142 618 40 120 957 360
Cuts at: 9889 5252
Size: 7467
Fragments arranged by size:
7467 1142 1075 957 618 360 325 120
40
RsrII CG'GwC_CG
Cuts at: 10299 10299
Size: 12104
SacI G_AGCT'C
Cuts at: 5958 7205 9118 5958
Size: 1247 1913 8944
Fragments arranged by size:
8944 1913 1247
SacII CC_GC'GG
Cuts at: 8297 8297
Size: 12104
SalI G'TCGA_C
Cuts at: 9141 9141
Size: 12104
SanDI GG'GwC_CC
Cuts at: 2015 2015
Size: 12104
SapI GCTCTTCn'nnn_
Cuts at: 3617 9038 10127 10337 3617
Size: 5421 1089 210 5384
Fragments arranged by size:
5421 5384 1089 210
SbfI CC_TGCA'GG
Cuts at: 9151 9151
Size: 12104
ScaI AGT'ACT
Cuts at: 11621 11621
Size: 12104
SexAI A'CCwGG_T
Cuts at: 4757 4757
Size: 12104
SfcI C'TryA_G
Cuts at: 33 1157 1657 2633 4341 4423 4800 5034 5453
Size: 1124 500 976 1708 82 377 234 419
Cuts at: 5453 8228 9002 9147 9832 10825 11016 33
Size: 2775 774 145 685 993 191 1121
Fragments arranged by size:
2775 1708 1124 1121 993 976 774 685
500 419 377 234 191 145 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 761 761
Size: 12104
SfoI GGC'GCC
Cuts at: 7117 8096 9536 9784 7117
Size: 979 1440 248 9437
Fragments arranged by size:
9437 1440 979 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12104
SmaI CCC'GGG
Cuts at: 1670 8807 9126 1670
Size: 7137 319 4648
Fragments arranged by size:
7137 4648 319
SmlI C'TyrA_G
Cuts at: 3469 3772 5135 5403 5939 6340 7449 9259 10174
Size: 303 1363 268 536 401 1109 1810 915
Cuts at: 10174 10666 10928 3469
Size: 492 262 4645
Fragments arranged by size:
4645 1810 1363 1109 915 536 492 401
303 268 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 12104
SphI G_CATG'C
Cuts at: 495 5340 9157 9657 495
Size: 4845 3817 500 2942
Fragments arranged by size:
4845 3817 2942 500
SspI AAT'ATT
Cuts at: 153 3396 3757 153
Size: 3243 361 8500
Fragments arranged by size:
8500 3243 361
StuI AGG'CCT
Cuts at: 176 1106 7139 7447 7571 8420 176
Size: 930 6033 308 124 849 3860
Fragments arranged by size:
6033 3860 930 849 308 124
StyI C'CwwG_G
Cuts at: 107 240 450 750 900 1433 1510 1560 1714
Size: 133 210 300 150 533 77 50 154
Cuts at: 1714 2084 2102 3153 3400 4679 5141 5176 5585
Size: 370 18 1051 247 1279 462 35 409
Cuts at: 5585 5753 6656 7134 7208 9238 9592 107
Size: 168 903 478 74 2030 354 2619
Fragments arranged by size:
2619 2030 1279 1051 903 533 478 462
409 370 354 300 247 210 168 154
150 133 77 74 50 35 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 466 1314 1745 1848 5130 6413 7173 7599 8071
Size: 848 431 103 3282 1283 760 426 472
Cuts at: 8071 9687 9820 10279 10488 466
Size: 1616 133 459 209 2082
Fragments arranged by size:
3282 2082 1616 1283 848 760 472 459
431 426 209 133 103
TatI w'GTAC_w
Cuts at: 3290 4201 5051 5342 5522 11619 3290
Size: 911 850 291 180 6097 3775
Fragments arranged by size:
6097 3775 911 850 291 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1320 1352 1562 1594 2792 2824 5183 5215 7239
Size: 32 210 32 1198 32 2359 32 2024
Cuts at: 7239 7271 11621 11653 1320
Size: 32 4350 32 1771
Fragments arranged by size:
4350 2359 2024 1771 1198 210 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 9901 9901
Size: 12104
XbaI T'CTAG_A
Cuts at: 1794 6895 9135 1794
Size: 5101 2240 4763
Fragments arranged by size:
5101 4763 2240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 457 1609 9599 457
Size: 1152 7990 2962
Fragments arranged by size:
7990 2962 1152
XhoI C'TCGA_G
Cuts at: 5135 5939 5135
Size: 804 11300
XmaI C'CCGG_G
Cuts at: 1668 8805 9124 1668
Size: 7137 319 4648
Fragments arranged by size:
7137 4648 319
XmnI GAAnn'nnTTC
Cuts at: 154 2707 3781 6148 154
Size: 2553 1074 2367 6110
Fragments arranged by size:
6110 2553 2367 1074
ZraI GAC'GTC
Cuts at: 3673 7703 3673
Size: 4030 8074
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BssHII BstZ17I ClaI
FseI FspAI NruI PacI PflMI PsiI SgrAI SnaBI
SrfI SwaI