Restriction map for pF1KE3388
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccI        AclI        AflII       AflIII      
AgeI        AocI        ApaI        AscI        AspI        
BbrPI       BbvCI       BcgI        BfrI        BsaAI       
BsiWI       Bsp1407I    BspDI       BspLU11I    BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DrdI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FseI        HpaI        KspI        
MluI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    RsrII       SacII       SalI        SapI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SspI        SwaI        
Tth111I     XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I       2563  GGTACC               G^GTACC
AccIII       1115  TCCGGA               T^CCGGA
AcyI           16  GGCGCC               G[GA]^CG[CT]C
AhdI         1660  GACGATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        744  GTGCAC               G^TGCAC
Aor51HI       608  AGCGCT               AGC^GCT
ApaLI         744  GTGCAC               G^TGCAC
AseI         2227  ATTAAT               AT^TAAT
AsnI         2227  ATTAAT               AT^TAAT
Asp718I      2563  GGTACC               G^GTACC
AspEI        1660  GACGATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3143  TGCGCA               TGC^GCA
AvrII        2085  CCTAGG               C^CTAGG
BalI         1604  TGGCCA               TGG^CCA
BbeI           16  GGCGCC               GGCGC^C
BciVI        2981  GTATCC               GTATCC(6/5)
BclI         2063  TGATCA               T^GATCA
BlnI         2085  CCTAGG               C^CTAGG
BsaBI        2709  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          16  GGCGCC               G[GA]^CG[CT]C
BsaI         1763  GAGACC               GGTCTC(1/5)
BseAI        1115  TCCGGA               T^CCGGA
BsmI         1902  GAATGC               GAATGC(1/-1)
BspEI        1115  TCCGGA               T^CCGGA
BspMI        2742  GCAGGT               ACCTGC(4/8)
BssSI        3355  CTCGTG               CACGAG(-5/-1)
DraI         3367  TTTAAA               TTT^AAA
EagI         1795  CGGCCG               C^GGCCG
Eam1105I     1660  GACGATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1795  CGGCCG               C^GGCCG
Eco47III      608  AGCGCT               AGC^GCT
Eco52I       1795  CGGCCG               C^GGCCG
EcoRV        2325  GATATC               GAT^ATC
FbaI         2063  TGATCA               T^GATCA
FspI         3143  TGCGCA               TGC^GCA
HgaI         1340  GCGTC                GACGC(5/10)
Hin1I          16  GGCGCC               G[GA]^CG[CT]C
HincII        331  GTCAAC               GT[CT]^[GA]AC
HindII        331  GTCAAC               GT[CT]^[GA]AC
HindIII       226  AAGCTT               A^AGCTT
KasI           16  GGCGCC               G^GCGCC
KpnI         2563  GGTACC               GGTAC^C
MamI         2709  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          492  CAATTG               C^AATTG
MluNI        1604  TGGCCA               TGG^CCA
MroI         1115  TCCGGA               T^CCGGA
MscI         1604  TGGCCA               TGG^CCA
MunI          492  CAATTG               C^AATTG
NarI           16  GGCGCC               GG^CGCC
NspI         2639  GCATGC               [GA]CATG^[CT]
PacI         2224  TTAATTAA             TTAAT^TAA
PshBI        2227  ATTAAT               AT^TAAT
PvuI          659  CGATCG               CGAT^CG
SbfI          783  CCTGCAGG             CCTGCA^GG
ScaI         1242  AGTACT               AGT^ACT
SfoI           16  GGCGCC               GGC^GCC
SphI         2639  GCATGC               GCATG^C
Sse8387I      783  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         513  CGCG                 CG^CG
             2350  CGCG                
AcsI          592  AAATTT               [GA]^AATT[CT]
             3346  AAATTC              
ApoI          592  AAATTT               [GA]^AATT[CT]
             3346  AAATTC              
Asp700I      1533  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3126  GAATAACTTC          
BamHI        3192  GGATCC               G^GATCC
             3590  GGATCC              
BanI           16  GGCGCC               G^G[CT][GA]CC
             2563  GGTACC              
BfaI         2086  CTAG                 C^TAG
             2263  CTAG                
BglII        1289  AGATCT               A^GATCT
             2708  AGATCT              
BlpI         1256  GCTCAGC              GC^T[ACGT]AGC
             3612  GCTGAGC             
Bpu1102I     1256  GCTCAGC              GC^T[ACGT]AGC
             3612  GCTGAGC             
BsaWI        1115  TCCGGA               [AT]^CCGG[AT]
             1759  ACCGGA              
BspHI        1061  TCATGA               T^CATGA
             2288  TCATGA              
BsrBI        1213  CCGCTC               CCGCTC(-3/-3)
             3538  GAGCGG              
BstUI         513  CGCG                 CG^CG
             2350  CGCG                
CelII        1256  GCTCAGC              GC^T[ACGT]AGC
             3612  GCTGAGC             
MaeI         2086  CTAG                 C^TAG
             2263  CTAG                
MvnI          513  CGCG                 CG^CG
             2350  CGCG                
NcoI          815  CCATGG               C^CATGG
             3056  CCATGG              
PaeR7I       1814  CTCGAG               C^TCGAG
             2657  CTCGAG              
RcaI         1061  TCATGA               T^CATGA
             2288  TCATGA              
XhoI         1814  CTCGAG               C^TCGAG
             2657  CTCGAG              
XmnI         1533  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3126  GAATAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
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