Restriction map for pF1KB9280
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AlwI        AocI        Aor51HI     ApaI        AseI        
AsnI        Asp700I     AspI        AviII       AvrII       
BalI        BamHI       BglI        BglII       BlnI        
BsaBI       BsaWI       BseAI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraII       DraIII      DsaI        
Eco47III    Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI         1360  AAATTC               [GA]^AATT[CT]
AflII         789  CTTAAG               C^TTAAG
AhdI          481  GACAAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1216  GTGCAC               G^TGCAC
ApaLI        1216  GTGCAC               G^TGCAC
ApoI         1360  AAATTC               [GA]^AATT[CT]
AscI          371  GGCGCGCC             GG^CGCGCC
AspEI         481  GACAAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        1576  GGGCTC               G[GA]GC[CT]^C
BbeI          472  GGCGCC               GGCGC^C
BbrPI        1213  CACGTG               CAC^GTG
BbvCI         182  CCTCAGC              CCTCAGC(-5/-2)
BcgI          533  CGATCATCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1233  GGATAC               GTATCC(6/5)
BfrI          789  CTTAAG               C^TTAAG
BsaAI        1213  CACGTG               [CT]AC^GT[GA]
BsaI          454  GAGACC               GGTCTC(1/5)
BsiEI          34  CGGCCG               CG[GA][CT]^CG
BspHI        1226  TCATGA               T^CATGA
BspMI          11  GCAGGT               ACCTGC(4/8)
BssHII        372  GCGCGC               G^CGCGC
BssSI          73  CACGAG               CACGAG(-5/-1)
BstBI         382  TTCGAA               TT^CGAA
BstXI         227  CCAACAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI           34  CGGCCG               [CT]^GGCC[GA]
EagI           34  CGGCCG               C^GGCCG
Eam1105I      481  GACAAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          34  CGGCCG               C^GGCCG
Eco52I         34  CGGCCG               C^GGCCG
EcoNI        1679  CCTCGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1053  GATATC               GAT^ATC
EcoT14I      1035  CCTTGG               C^C[AT][AT]GG
HphI         1084  GGTGA                GGTGA(8/7)
KasI          472  GGCGCC               G^GCGCC
NarI          472  GGCGCC               GG^CGCC
NotI           33  GCGGCCGC             GC^GGCCGC
NspI          425  GCATGT               [GA]CATG^[CT]
NspV          382  TTCGAA               TT^CGAA
PflMI          72  CCACGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1213  CACGTG               CAC^GTG
PmlI         1213  CACGTG               CAC^GTG
RcaI         1226  TCATGA               T^CATGA
SfoI          472  GGCGCC               GGC^GCC
SfuI          382  TTCGAA               TT^CGAA
SgrAI         367  CACCGGCG             C[GA]^CCGG[CT]G
StyI         1035  CCTTGG               C^C[AT][AT]GG
Van91I         72  CCACGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          853  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Acc65I       1001  GGTACC               G^GTACC
             1092  GGTACC              
AflIII        892  ACACGT               A^C[GA][CT]GT
             1212  ACACGT              
Asp718I      1001  GGTACC               G^GTACC
             1092  GGTACC              
AvaI          190  CCCGAG               C^[CT]CG[GA]G
              211  CCCGAG              
BbsI          906  GAAGAC               GAAGAC(2/6)
             1501  GAAGAC              
BclI          551  TGATCA               T^GATCA
             1008  TGATCA              
BcnI          716  CCCGG                CC^[GC]GG
             1673  CCCGG               
BpmI          269  CTGGAG               CTGGAG(16/14)
              612  CTGGAG              
BpuAI         906  GAAGAC               GAAGAC(2/6)
             1501  GAAGAC              
BsmFI          57  GTCCC                GGGAC(10/14)
             1671  GTCCC               
BsoBI         190  CCCGAG               C^[CT]CG[GA]G
              211  CCCGAG              
BsrFI         250  ACCGGC               [GA]^CCGG[CT]
              368  ACCGGC              
BstAPI         93  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              151  GCAGCCGCTGC         
Cfr10I        250  ACCGGC               [GA]^CCGG[CT]
              368  ACCGGC              
DrdI          456  GACCGAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1642  GACAAGCACGTC        
EarI         1701  CTCTTC               CTCTTC(1/4)
             1512  GAAGAG              
FbaI          551  TGATCA               T^GATCA
             1008  TGATCA              
HaeII         237  GGCGCT               [GA]GCGC^[CT]
              472  GGCGCC              
KpnI         1001  GGTACC               GGTAC^C
             1092  GGTACC              
Ksp632I      1701  CTCTTC               CTCTTC(1/4)
             1512  GAAGAG              
MslI          701  CATTCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1431  CACTTTGGTG          
NciI          716  CCCGG                CC^[GC]GG
             1673  CCCGG               
SmlI           48  CTTGAG               C^T[CT][GA]AG
              789  CTTAAG              
Tsp45I       1085  GTGAC                ^GT[GC]AC
             1651  GTCAC               
XcmI          749  CCACCGCTGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1563  CCACGCCCTCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaII       BanI        BbvI        
BfaI        BlpI        BmyI        Bpu1102I    BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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