Restriction map for pF1KB9280
 (Linear) (Six-base) MAPSORT of: KIBB9280.seq  Check: 5772  from: 1  to: 1737



KIBB9280.orf

 With 182 enzymes: SgfI * 

                        September 22, 2011 10:48  ..

Acc65I G'GTAC_C

Cuts at:      0    1001    1092    1737 
   Size:       1001      91     645

  Fragments arranged by size: 

                1001     645      91
 
AflII C'TTAA_G

Cuts at:      0     789    1737 
   Size:        789     948
 
AflIII A'CryG_T

Cuts at:      0     892    1212    1737 
   Size:        892     320     525

  Fragments arranged by size: 

                 892     525     320
 
AhdI GACnn_n'nnGTC

Cuts at:      0     486    1737 
   Size:        486    1251
 
AleI CACnn'nnGTG

Cuts at:      0    1435    1737 
   Size:       1435     302
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     157     934    1737 
   Size:         99      58     777     803

  Fragments arranged by size: 

                 803     777      99      58
 
ApaLI G'TGCA_C

Cuts at:      0    1216    1737 
   Size:       1216     521
 
ApoI r'AATT_y

Cuts at:      0    1360    1737 
   Size:       1360     377
 
AscI GG'CGCG_CC

Cuts at:      0     372    1737 
   Size:        372    1365
 
AvaI C'yCGr_G

Cuts at:      0     190     211    1737 
   Size:        190      21    1526

  Fragments arranged by size: 

                1526     190      21
 
BanI G'GyrC_C

Cuts at:      0     316     472    1001    1092    1737 
   Size:        316     156     529      91     645

  Fragments arranged by size: 

                 645     529     316     156      91
 
BanII G_rGCy'C

Cuts at:      0    1580    1737 
   Size:       1580     157
 
BbeI G_GCGC'C

Cuts at:      0     476    1737 
   Size:        476    1261
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     913    1508    1737 
   Size:        913     595     229

  Fragments arranged by size: 

                 913     595     229
 
BbvCI CC'TCA_GC

Cuts at:      0     183    1737 
   Size:        183    1554
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     522     556    1737 
   Size:        522      34    1181

  Fragments arranged by size: 

                1181     522      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1227    1737 
   Size:       1227     510
 
BclI T'GATC_A

Cuts at:      0     551    1008    1737 
   Size:        551     457     729

  Fragments arranged by size: 

                 729     551     457
 
BlpI GC'TnA_GC

Cuts at:      0      40     400    1664    1737 
   Size:         40     360    1264      73

  Fragments arranged by size: 

                1264     360      73      40
 
Bme1580I G_kGCm'C

Cuts at:      0     302    1220    1737 
   Size:        302     918     517

  Fragments arranged by size: 

                 918     517     302
 
BmgBI CAC'GTC

Cuts at:      0    1650    1737 
   Size:       1650      87
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      99    1737 
   Size:         99    1638
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     633    1737 
   Size:        290     343    1104

  Fragments arranged by size: 

                1104     343     290
 
Bpu10I CC'TnA_GC

Cuts at:      0     183     501    1737 
   Size:        183     318    1236

  Fragments arranged by size: 

                1236     318     183
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69    1737 
   Size:         69    1668
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     448    1737 
   Size:        448    1289
 
BsaAI yAC'GTr

Cuts at:      0    1215    1737 
   Size:       1215     522
 
BsaHI Gr'CG_yC

Cuts at:      0      26     473     487    1737 
   Size:         26     447      14    1250

  Fragments arranged by size: 

                1250     447      26      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     208     238    1737 
   Size:        208      30    1499

  Fragments arranged by size: 

                1499     208      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     287     840    1737 
   Size:        137     150     553     897

  Fragments arranged by size: 

                 897     553     150     137
 
BseYI C'CCAG_C

Cuts at:      0     360    1737 
   Size:        360    1377
 
BsiEI CG_ry'CG

Cuts at:      0      37    1737 
   Size:         37    1700
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     870    1220    1737 
   Size:        218     652     350     517

  Fragments arranged by size: 

                 652     517     350     218
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     302     870    1220    1580    1737 
   Size:        218      84     568     350     360     157

  Fragments arranged by size: 

                 568     360     350     218     157      84
 
BspHI T'CATG_A

Cuts at:      0    1226    1737 
   Size:       1226     511
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       2    1737 
   Size:          2    1735
 
BsrBI CCG'CTC

Cuts at:      0      39     296    1668    1737 
   Size:         39     257    1372      69

  Fragments arranged by size: 

                1372     257      69      39
 
BsrFI r'CCGG_y

Cuts at:      0     250     368    1737 
   Size:        250     118    1369

  Fragments arranged by size: 

                1369     250     118
 
BssHII G'CGCG_C

Cuts at:      0     372    1737 
   Size:        372    1365
 
BssSI C'ACGA_G

Cuts at:      0      73    1737 
   Size:         73    1664
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     157    1737 
   Size:         99      58    1580

  Fragments arranged by size: 

                1580      99      58
 
BstBI TT'CG_AA

Cuts at:      0     383    1737 
   Size:        383    1354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     234    1737 
   Size:        234    1503
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1057    1737 
   Size:       1057     680
 
BtsI GCAGTG_nn'

Cuts at:      0     318    1737 
   Size:        318    1419
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     462    1648    1737 
   Size:        462    1186      89

  Fragments arranged by size: 

                1186     462      89
 
EaeI y'GGCC_r

Cuts at:      0      34    1737 
   Size:         34    1703
 
EagI C'GGCC_G

Cuts at:      0      34    1737 
   Size:         34    1703
 
EarI CTCTTCn'nnn_

Cuts at:      0    1507    1707    1737 
   Size:       1507     200      30

  Fragments arranged by size: 

                1507     200      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     272    1737 
   Size:        272    1465
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     633    1737 
   Size:        290     343    1104

  Fragments arranged by size: 

                1104     343     290
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1683    1737 
   Size:       1683      54
 
EcoRV GAT'ATC

Cuts at:      0    1055    1737 
   Size:       1055     682
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     900    1737 
   Size:        900     837
 
HaeII r_GCGC'y

Cuts at:      0     241     476    1737 
   Size:        241     235    1261

  Fragments arranged by size: 

                1261     241     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504     835     867    1221    1253    1737 
   Size:        472      32     331      32     354      32     484

  Fragments arranged by size: 

                 484     472     354     331      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     472    1737 
   Size:        472    1265
 
KpnI G_GTAC'C

Cuts at:      0    1005    1096    1737 
   Size:       1005      91     641

  Fragments arranged by size: 

                1005     641      91
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     251    1737 
   Size:         99     152    1486

  Fragments arranged by size: 

                1486     152      99
 
MslI CAynn'nnrTG

Cuts at:      0     705    1435    1737 
   Size:        705     730     302

  Fragments arranged by size: 

                 730     705     302
 
MspA1I CmG'CkG

Cuts at:      0       8     157     172     754    1242    1267    1737 
   Size:          8     149      15     582     488      25     470

  Fragments arranged by size: 

                 582     488     470     149      25      15       8
 
NarI GG'CG_CC

Cuts at:      0     473    1737 
   Size:        473    1264
 
NotI GC'GGCC_GC

Cuts at:      0      34    1737 
   Size:         34    1703
 
NspI r_CATG'y

Cuts at:      0     429    1737 
   Size:        429    1308
 
PasI CC'CwG_GG

Cuts at:      0    1573    1737 
   Size:       1573     164
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78    1737 
   Size:         78    1659
 
PfoI T'CCnGG_A

Cuts at:      0    1684    1737 
   Size:       1684      53
 
PmlI CAC'GTG

Cuts at:      0    1215    1737 
   Size:       1215     522
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     337     369    1737 
   Size:        337      32    1368

  Fragments arranged by size: 

                1368     337      32
 
PstI C_TGCA'G

Cuts at:      0      13     104     153    1737 
   Size:         13      91      49    1584

  Fragments arranged by size: 

                1584      91      49      13
 
PvuII CAG'CTG

Cuts at:      0       8    1242    1267    1737 
   Size:          8    1234      25     470

  Fragments arranged by size: 

                1234     470      25       8
 
SfcI C'TryA_G

Cuts at:      0       9     100     149    1172    1737 
   Size:          9      91      49    1023     565

  Fragments arranged by size: 

                1023     565      91      49       9
 
SfoI GGC'GCC

Cuts at:      0     474    1737 
   Size:        474    1263
 
SgrAI Cr'CCGG_yG

Cuts at:      0     368    1737 
   Size:        368    1369
 
SmlI C'TyrA_G

Cuts at:      0      48     789    1737 
   Size:         48     741     948

  Fragments arranged by size: 

                 948     741      48
 
StyI C'CwwG_G

Cuts at:      0    1035    1737 
   Size:       1035     702
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     472     535    1132    1737 
   Size:        472      63     597     605

  Fragments arranged by size: 

                 605     597     472      63
 
TatI w'GTAC_w

Cuts at:      0     936    1275    1737 
   Size:        936     339     462

  Fragments arranged by size: 

                 936     462     339
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     208     240    1737 
   Size:        208      32    1497

  Fragments arranged by size: 

                1497     208      32
 
XbaI T'CTAG_A

Cuts at:      0     853    1737 
   Size:        853     884
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     756    1570    1737 
   Size:        756     814     167

  Fragments arranged by size: 

                 814     756     167
 

 Enzymes that do cut:

   Acc65I    AflII   AflIII     AhdI     AleI    AlwNI    ApaLI     ApoI
     AscI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BtgZI     BtsI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRV     FalI    HaeII    Hin4I
     KasI     KpnI     MmeI     MslI   MspA1I     NarI     NotI     NspI
     PasI    PflMI     PfoI     PmlI     PsrI     PstI    PvuII     SfcI
     SfoI    SgrAI     SmlI     StyI    TaqII     TatI     TstI     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI     AgeI     AlfI
     AloI     ApaI     AseI    AsiSI    AvrII     BaeI    BamHI    BfrBI
     BglI    BglII     BmrI     BmtI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII  EcoICRI EcoO109I
    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI
     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XhoI
     XmaI     XmnI     ZraI
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