Restriction map for pF1KE2373
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BcgI        
BclI        BfrI        BglII       BlnI        BsiWI       
BsmI        BspDI       BspHI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    EcoRI       EcoRV       FbaI        FseI        
FspI        HincII      HindII      HpaI        KpnI        
MfeI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tru9I       XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI         1556  AAATTC               [GA]^AATT[CT]
Alw44I        624  GTGCAC               G^TGCAC
ApaLI         624  GTGCAC               G^TGCAC
ApoI         1556  AAATTC               [GA]^AATT[CT]
Asp700I      3375  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1047  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2545  CCCGAG               C^[CT]CG[GA]G
BamHI        1352  GGATCC               G^GATCC
BbrPI        3424  CACGTG               CAC^GTG
BciVI        2913  GGATAC               GTATCC(6/5)
BlpI         2360  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2360  GCTCAGC              GC^T[ACGT]AGC
BsaAI        3424  CACGTG               [CT]AC^GT[GA]
BsaBI        1773  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1897  CGGCCG               CG[GA][CT]^CG
BsoBI        2545  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     3173  TGTACA               T^GTACA
BspLU11I     3026  ACATGT               A^CATGT
BsrGI        3173  TGTACA               T^GTACA
BssSI        2182  CACGAG               CACGAG(-5/-1)
CelII        2360  GCTCAGC              GC^T[ACGT]AGC
DraIII        532  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1897  CGGCCG               C^GGCCG
EclXI        1897  CGGCCG               C^GGCCG
Eco52I       1897  CGGCCG               C^GGCCG
EcoNI        2946  CCTGAGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1305  ATGCAT               ATGCA^T
HindIII      2320  AAGCTT               A^AGCTT
MamI         1773  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          103  ACGCGT               A^CGCGT
NotI         1896  GCGGCCGC             GC^GGCCGC
NsiI         1305  ATGCAT               ATGCA^T
PflFI        1047  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3424  CACGTG               CAC^GTG
PmlI         3424  CACGTG               CAC^GTG
SphI         3222  GCATGC               GCATG^C
SspBI        3173  TGTACA               T^GTACA
Tth111I      1047  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         3375  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccIII       1298  TCCGGA               T^CCGGA
             1372  TCCGGA              
AocI         1086  CCTGAGG              CC^T[ACGT]AGG
             1123  CCTGAGG             
Aor51HI       273  AGCGCT               AGC^GCT
             1217  AGCGCT              
BbeI         2051  GGCGCC               GGCGC^C
             2506  GGCGCC              
BseAI        1298  TCCGGA               T^CCGGA
             1372  TCCGGA              
BsmBI         100  GAGACG               CGTCTC(1/5)
             2597  GAGACG              
BspEI        1298  TCCGGA               T^CCGGA
             1372  TCCGGA              
BsrDI        1323  CATTGC               GCAATG(2/0)
              971  GCAATG              
BstAPI       1416  GCAACGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1991  GCAAGTGGTGC         
BstEII       1135  GGTGACC              G^GT[ACGT]ACC
             1918  GGTGACC             
BstPI        1135  GGTGACC              G^GT[ACGT]ACC
             1918  GGTGACC             
BstYI         716  AGATCC               [GA]^GATC[CT]
             1352  GGATCC              
Bsu36I       1086  CCTGAGG              CC^T[ACGT]AGG
             1123  CCTGAGG             
DrdI          910  GACCTCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1138  GACCTCCTCGTC        
Eco47III      273  AGCGCT               AGC^GCT
             1217  AGCGCT              
Eco81I       1086  CCTGAGG              CC^T[ACGT]AGG
             1123  CCTGAGG             
EcoO65I      1135  GGTGACC              G^GT[ACGT]ACC
             1918  GGTGACC             
EcoT14I        71  CCTTGG               C^C[AT][AT]GG
             2930  CCAAGG              
KasI         2051  GGCGCC               G^GCGCC
             2506  GGCGCC              
KspI          377  CCGCGG               CCGC^GG
             1075  CCGCGG              
MflI          716  AGATCC               [GA]^GATC[CT]
             1352  GGATCC              
MroI         1298  TCCGGA               T^CCGGA
             1372  TCCGGA              
NarI         2051  GGCGCC               GG^CGCC
             2506  GGCGCC              
NheI         1737  GCTAGC               G^CTAGC
             2787  GCTAGC              
NspI         3026  ACATGT               [GA]CATG^[CT]
             3222  GCATGC              
PflMI         534  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3280  CCACAGGATGG         
PshAI        1332  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3333  GACATCTGTC          
SacII         377  CCGCGG               CCGC^GG
             1075  CCGCGG              
SexAI         533  ACCAGGT              A^CC[AT]GGT
             2672  ACCTGGT             
SfiI         1468  GGCCGGGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1477  GGCCGCACGGGCC       
SfoI         2051  GGCGCC               GGC^GCC
             2506  GGCGCC              
SmlI          217  CTCAAG               C^T[CT][GA]AG
              913  CTCAAG              
StuI           68  AGGCCT               AGG^CCT
             3365  AGGCCT              
StyI           71  CCTTGG               C^C[AT][AT]GG
             2930  CCAAGG              
Van91I        534  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3280  CCACAGGATGG         
XhoII         716  AGATCC               [GA]^GATC[CT]
             1352  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        
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