Restriction map for pF1KE2373
 (Linear) (Six-base) MAPSORT of: KIEE2373.seq  Check: 4021  from: 1  to: 3429



KIEE2373.orf

 With 182 enzymes: SgfI * 

                        September 6, 2011 14:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1950    2533    3274    3429 
   Size:       1950     583     741     155

  Fragments arranged by size: 

                1950     741     583     155
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     594    2419    3429 
   Size:        544      50    1825    1010

  Fragments arranged by size: 

                1825    1010     544      50
 
AfeI AGC'GCT

Cuts at:      0     275    1219    3429 
   Size:        275     944    2210

  Fragments arranged by size: 

                2210     944     275
 
AflIII A'CryG_T

Cuts at:      0     103     733    3026    3429 
   Size:        103     630    2293     403

  Fragments arranged by size: 

                2293     630     403     103
 
AleI CACnn'nnGTG

Cuts at:      0     580    1018    3400    3429 
   Size:        580     438    2382      29

  Fragments arranged by size: 

                2382     580     438      29
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     388    2230    2369    3429 
   Size:        388    1842     139    1060

  Fragments arranged by size: 

                1842    1060     388     139
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2299    2331    3429 
   Size:       2299      32    1098

  Fragments arranged by size: 

                2299    1098      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     360     522     594     891    2373    2568    2582    2895 
   Size:        360     162      72     297    1482     195      14     313

Cuts at:   2895    3130    3182    3301    3429 
   Size:        235      52     119     128

  Fragments arranged by size: 

                1482     360     313     297     235     195     162     128
                 119      72      52      14
 
ApaI G_GGCC'C

Cuts at:      0    1489    1698    2100    2160    3293    3429 
   Size:       1489     209     402      60    1133     136

  Fragments arranged by size: 

                1489    1133     402     209     136      60
 
ApaLI G'TGCA_C

Cuts at:      0     624    3429 
   Size:        624    2805
 
ApoI r'AATT_y

Cuts at:      0    1556    3429 
   Size:       1556    1873
 
AvaI C'yCGr_G

Cuts at:      0    2545    3429 
   Size:       2545     884
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2906    2939    3429 
   Size:       2906      33     490

  Fragments arranged by size: 

                2906     490      33
 
BamHI G'GATC_C

Cuts at:      0    1352    3429 
   Size:       1352    2077
 
BanI G'GyrC_C

Cuts at:      0     582     990    1284    1997    2051    2506    3429 
   Size:        582     408     294     713      54     455     923

  Fragments arranged by size: 

                 923     713     582     455     408     294      54
 
BanII G_rGCy'C

Cuts at:      0      54     352     408    1489    1643    1698    1789    2100 
   Size:         54     298      56    1081     154      55      91     311

Cuts at:   2100    2160    3293    3429 
   Size:         60    1133     136

  Fragments arranged by size: 

                1133    1081     311     298     154     136      91      60
                  56      55      54
 
BbeI G_GCGC'C

Cuts at:      0    2055    2510    3429 
   Size:       2055     455     919

  Fragments arranged by size: 

                2055     919     455
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1140    1935    2523    3337    3429 
   Size:       1140     795     588     814      92

  Fragments arranged by size: 

                1140     814     795     588      92
 
BbvCI CC'TCA_GC

Cuts at:      0    1153    2635    3302    3429 
   Size:       1153    1482     667     127

  Fragments arranged by size: 

                1482    1153     667     127
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2907    3429 
   Size:       2907     522
 
BfrBI ATG'CAT

Cuts at:      0    1307    3429 
   Size:       1307    2122
 
BglI GCCn_nnn'nGGC

Cuts at:      0     903     999    1475    1484    2009    3080    3429 
   Size:        903      96     476       9     525    1071     349

  Fragments arranged by size: 

                1071     903     525     476     349      96       9
 
BlpI GC'TnA_GC

Cuts at:      0    2361    3429 
   Size:       2361    1068
 
Bme1580I G_kGCm'C

Cuts at:      0     587     628    1489    1698    2100    2160    2844    2859 
   Size:        587      41     861     209     402      60     684      15

Cuts at:   2859    3293    3429 
   Size:        434     136

  Fragments arranged by size: 

                 861     684     587     434     402     209     136      60
                  41      15
 
BmgBI CAC'GTC

Cuts at:      0    1052    3429 
   Size:       1052    2377
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     370     489     582     807    3429 
   Size:        370     119      93     225    2622

  Fragments arranged by size: 

                2622     370     225     119      93
 
BmtI G_CTAG'C

Cuts at:      0    1741    2791    3429 
   Size:       1741    1050     638

  Fragments arranged by size: 

                1741    1050     638
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     868     942    1117    1204    1313    1924    2229 
   Size:        210     658      74     175      87     109     611     305

Cuts at:   2229    2590    2674    2686    2797    2917    3262    3310    3429 
   Size:        361      84      12     111     120     345      48     119

  Fragments arranged by size: 

                 658     611     361     345     305     210     175     120
                 119     111     109      87      84      74      48      12
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1153    2374    2635    2947    3302    3429 
   Size:       1153    1221     261     312     355     127

  Fragments arranged by size: 

                1221    1153     355     312     261     127
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     898    3429 
   Size:        202     696    2531

  Fragments arranged by size: 

                2531     696     202
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      32    1324    2650    3429 
   Size:         32    1292    1326     779

  Fragments arranged by size: 

                1326    1292     779      32
 
BsaAI yAC'GTr

Cuts at:      0    3426    3429 
   Size:       3426       3
 
BsaBI GATnn'nnATC

Cuts at:      0    1777    3429 
   Size:       1777    1652
 
BsaHI Gr'CG_yC

Cuts at:      0     450     878    2052    2447    2507    3429 
   Size:        450     428    1174     395      60     922

  Fragments arranged by size: 

                1174     922     450     428     395      60
 
BsaWI w'CCGG_w

Cuts at:      0    1298    1372    3413    3429 
   Size:       1298      74    2041      16

  Fragments arranged by size: 

                2041    1298      74      16
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2227    2257    2615    2645    3429 
   Size:       2227      30     358      30     784

  Fragments arranged by size: 

                2227     784     358      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250    1132    1141    2032    2200    2242    2623    2914 
   Size:        250     882       9     891     168      42     381     291

Cuts at:   2914    2917    2920    2923    3429 
   Size:          3       3       3     506

  Fragments arranged by size: 

                 891     882     506     381     291     250     168      42
                   9       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      55     270     629     802    1214    1220    1742    2092 
   Size:         55     215     359     173     412       6     522     350

Cuts at:   2092    2268    2409    2846    2885    3051    3429 
   Size:        176     141     437      39     166     378

  Fragments arranged by size: 

                 522     437     412     378     359     350     215     176
                 173     166     141      55      39       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1222    1978    2146    2500    2539    3429 
   Size:       1222     756     168     354      39     890

  Fragments arranged by size: 

                1222     890     756     354     168      39
 
BsiEI CG_ry'CG

Cuts at:      0    1900    3429 
   Size:       1900    1529
 
BsiHKAI G_wGCw'C

Cuts at:      0     533     628     733    1817    3429 
   Size:        533      95     105    1084    1612

  Fragments arranged by size: 

                1612    1084     533     105      95
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      94    2591    3429 
   Size:         94    2497     838

  Fragments arranged by size: 

                2497     838      94
 
Bsp1286I G_dGCh'C

Cuts at:      0      54     352     408     533     587     628     733    1489 
   Size:         54     298      56     125      54      41     105     756

Cuts at:   1489    1643    1698    1789    1817    2100    2160    2844    2859 
   Size:        154      55      91      28     283      60     684      15

Cuts at:   2859    3293    3429 
   Size:        434     136

  Fragments arranged by size: 

                 756     684     434     298     283     154     136     125
                 105      91      60      56      55      54      54      41
                  28      15
 
BspEI T'CCGG_A

Cuts at:      0    1298    1372    3429 
   Size:       1298      74    2057

  Fragments arranged by size: 

                2057    1298      74
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     602    1950    2100    2533    3211    3274    3429 
   Size:        602    1348     150     433     678      63     155

  Fragments arranged by size: 

                1348     678     602     433     155     150      63
 
BsrBI CCG'CTC

Cuts at:      0     474     849    1131    3429 
   Size:        474     375     282    2298

  Fragments arranged by size: 

                2298     474     375     282
 
BsrDI GCAATG_nn'

Cuts at:      0     978    1322    3429 
   Size:        978     344    2107

  Fragments arranged by size: 

                2107     978     344
 
BsrFI r'CCGG_y

Cuts at:      0     550     559    2723    2864    3429 
   Size:        550       9    2164     141     565

  Fragments arranged by size: 

                2164     565     550     141       9
 
BsrGI T'GTAC_A

Cuts at:      0    3173    3429 
   Size:       3173     256
 
BssSI C'ACGA_G

Cuts at:      0    2182    3429 
   Size:       2182    1247
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1422    1997    3429 
   Size:       1422     575    1432

  Fragments arranged by size: 

                1432    1422     575
 
BstEII G'GTnAC_C

Cuts at:      0    1135    1918    3429 
   Size:       1135     783    1511

  Fragments arranged by size: 

                1511    1135     783
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     669    2937    3070    3429 
   Size:        669    2268     133     359

  Fragments arranged by size: 

                2268     669     359     133
 
BstYI r'GATC_y

Cuts at:      0     716    1352    3429 
   Size:        716     636    2077

  Fragments arranged by size: 

                2077     716     636
 
Bsu36I CC'TnA_GG

Cuts at:      0    1087    1124    3429 
   Size:       1087      37    2305

  Fragments arranged by size: 

                2305    1087      37
 
BtgI C'CryG_G

Cuts at:      0     377    1075    2079    3429 
   Size:        377     698    1004    1350

  Fragments arranged by size: 

                1350    1004     698     377
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     474    1806    3234    3429 
   Size:        474    1332    1428     195

  Fragments arranged by size: 

                1428    1332     474     195
 
BtsI GCAGTG_nn'

Cuts at:      0    1234    3357    3429 
   Size:       1234    2123      72

  Fragments arranged by size: 

                2123    1234      72
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     735     770    1305    1340    3429 
   Size:        735      35     535      35    2089

  Fragments arranged by size: 

                2089     735     535      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     537    3429 
   Size:        537    2892
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     916    1144    3429 
   Size:        916     228    2285

  Fragments arranged by size: 

                2285     916     228
 
EaeI y'GGCC_r

Cuts at:      0     278     429     455     548     659     743     932    1256 
   Size:        278     151      26      93     111      84     189     324

Cuts at:   1256    1897    2039    2435    2762    2939    3429 
   Size:        641     142     396     327     177     490

  Fragments arranged by size: 

                 641     490     396     327     324     278     189     177
                 151     142     111      93      84      26
 
EagI C'GGCC_G

Cuts at:      0    1897    3429 
   Size:       1897    1532
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     131     958    1591    1939    3429 
   Size:         59      72     827     633     348    1490

  Fragments arranged by size: 

                1490     827     633     348      72      59
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     388     654    1054    1180    1492    3429 
   Size:        388     266     400     126     312    1937

  Fragments arranged by size: 

                1937     400     388     312     266     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     544     594     868     942    1117    1204    1313 
   Size:        210     334      50     274      74     175      87     109

Cuts at:   1313    1924    2229    2419    2590    2674    2686    2797    2917 
   Size:        611     305     190     171      84      12     111     120

Cuts at:   2917    3262    3310    3429 
   Size:        345      48     119

  Fragments arranged by size: 

                 611     345     334     305     274     210     190     175
                 171     120     119     111     109      87      84      74
                  50      48      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2950    3429 
   Size:       2950     479
 
EcoO109I rG'GnC_Cy

Cuts at:      0     822    1486    1614    1694    1695    1713    1730    2096 
   Size:        822     664     128      80       1      18      17     366

Cuts at:   2096    2157    2370    2418    3037    3276    3290    3350    3429 
   Size:         61     213      48     619     239      14      60      79

  Fragments arranged by size: 

                 822     664     619     366     239     213     128      80
                  79      61      60      48      18      17      14       1
            
 
HaeII r_GCGC'y

Cuts at:      0     277     891    1178    1221    1247    1865    2055    2145 
   Size:        277     614     287      43      26     618     190      90

Cuts at:   2145    2154    2510    3429 
   Size:          9     356     919

  Fragments arranged by size: 

                 919     618     614     356     287     277     190      90
                  43      26       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     672     704    1383    1415    2227    2259    3429 
   Size:        672      32     679      32     812      32    1170

  Fragments arranged by size: 

                1170     812     679     672      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    2320    3429 
   Size:       2320    1109
 
KasI G'GCGC_C

Cuts at:      0    2051    2506    3429 
   Size:       2051     455     923

  Fragments arranged by size: 

                2051     923     455
 
MluI A'CGCG_T

Cuts at:      0     103    3429 
   Size:        103    3326
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2051    2835    3429 
   Size:       2051     784     594

  Fragments arranged by size: 

                2051     784     594
 
MscI TGG'CCA

Cuts at:      0     431     457     661    2041    2941    3429 
   Size:        431      26     204    1380     900     488

  Fragments arranged by size: 

                1380     900     488     431     204      26
 
MslI CAynn'nnrTG

Cuts at:      0     580     925    1018    1606    3031    3400    3429 
   Size:        580     345      93     588    1425     369      29

  Fragments arranged by size: 

                1425     588     580     369     345      93      29
 
MspA1I CmG'CkG

Cuts at:      0     379     795    1077    1157    1461    1746    1902    2169 
   Size:        379     416     282      80     304     285     156     267

Cuts at:   2169    2343    2353    2388    2433    2846    2871    2895    3429 
   Size:        174      10      35      45     413      25      24     534

  Fragments arranged by size: 

                 534     416     413     379     304     285     282     267
                 174     156      80      45      35      25      24      10
            
 
NarI GG'CG_CC

Cuts at:      0    2052    2507    3429 
   Size:       2052     455     922

  Fragments arranged by size: 

                2052     922     455
 
NheI G'CTAG_C

Cuts at:      0    1737    2787    3429 
   Size:       1737    1050     642

  Fragments arranged by size: 

                1737    1050     642
 
NotI GC'GGCC_GC

Cuts at:      0    1897    3429 
   Size:       1897    1532
 
NsiI A_TGCA'T

Cuts at:      0    1309    3429 
   Size:       1309    2120
 
NspI r_CATG'y

Cuts at:      0    3030    3226    3429 
   Size:       3030     196     203

  Fragments arranged by size: 

                3030     203     196
 
PasI CC'CwG_GG

Cuts at:      0    2278    3295    3429 
   Size:       2278    1017     134

  Fragments arranged by size: 

                2278    1017     134
 
PciI A'CATG_T

Cuts at:      0    3026    3429 
   Size:       3026     403
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     540    3286    3429 
   Size:        540    2746     143

  Fragments arranged by size: 

                2746     540     143
 
PfoI T'CCnGG_A

Cuts at:      0     226    1550    1682    2303    3429 
   Size:        226    1324     132     621    1126

  Fragments arranged by size: 

                1324    1126     621     226     132
 
PmlI CAC'GTG

Cuts at:      0    3426    3429 
   Size:       3426       3
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     720     752    3429 
   Size:        720      32    2677

  Fragments arranged by size: 

                2677     720      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     822    1614    1730    2370    3037    3429 
   Size:        822     792     116     640     667     392

  Fragments arranged by size: 

                 822     792     667     640     392     116
 
PshAI GACnn'nnGTC

Cuts at:      0    1336    3337    3429 
   Size:       1336    2001      92

  Fragments arranged by size: 

                2001    1336      92
 
PspOMI G'GGCC_C

Cuts at:      0    1485    1694    2096    2156    3289    3429 
   Size:       1485     209     402      60    1133     140

  Fragments arranged by size: 

                1485    1133     402     209     140      60
 
PstI C_TGCA'G

Cuts at:      0     203     467     518    1427    1592    2225    2348    2786 
   Size:        203     264      51     909     165     633     123     438

Cuts at:   2786    3347    3429 
   Size:        561      82

  Fragments arranged by size: 

                 909     633     561     438     264     203     165     123
                  82      51
 
PvuII CAG'CTG

Cuts at:      0    1746    2169    2343    2388    2433    2871    2895    3429 
   Size:       1746     423     174      45      45     438      24     534

  Fragments arranged by size: 

                1746     534     438     423     174      45      45      24
            
 
SacII CC_GC'GG

Cuts at:      0     380    1078    3429 
   Size:        380     698    2351

  Fragments arranged by size: 

                2351     698     380
 
SanDI GG'GwC_CC

Cuts at:      0    1614    1730    3037    3429 
   Size:       1614     116    1307     392

  Fragments arranged by size: 

                1614    1307     392     116
 
SapI GCTCTTCn'nnn_

Cuts at:      0     958    1591    1939    3429 
   Size:        958     633     348    1490

  Fragments arranged by size: 

                1490     958     633     348
 
SexAI A'CCwGG_T

Cuts at:      0     533    2672    3429 
   Size:        533    2139     757

  Fragments arranged by size: 

                2139     757     533
 
SfcI C'TryA_G

Cuts at:      0     199     463     514    1423    1588    2124    2221    2344 
   Size:        199     264      51     909     165     536      97     123

Cuts at:   2344    2782    3343    3429 
   Size:        438     561      86

  Fragments arranged by size: 

                 909     561     536     438     264     199     165     123
                  97      86      51
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1475    1484    3429 
   Size:       1475       9    1945

  Fragments arranged by size: 

                1945    1475       9
 
SfoI GGC'GCC

Cuts at:      0    2053    2508    3429 
   Size:       2053     455     921

  Fragments arranged by size: 

                2053     921     455
 
SmlI C'TyrA_G

Cuts at:      0     217     913    3429 
   Size:        217     696    2516

  Fragments arranged by size: 

                2516     696     217
 
SphI G_CATG'C

Cuts at:      0    3226    3429 
   Size:       3226     203
 
StuI AGG'CCT

Cuts at:      0      70    3367    3429 
   Size:         70    3297      62

  Fragments arranged by size: 

                3297      70      62
 
StyI C'CwwG_G

Cuts at:      0      71    2930    3429 
   Size:         71    2859     499

  Fragments arranged by size: 

                2859     499      71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     643    1212    1228    1737    1944    3408    3429 
   Size:        643     569      16     509     207    1464      21

  Fragments arranged by size: 

                1464     643     569     509     207      21      16
 
TatI w'GTAC_w

Cuts at:      0    3173    3429 
   Size:       3173     256
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1823    1855    3429 
   Size:       1823      32    1574

  Fragments arranged by size: 

                1823    1574      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1050    3429 
   Size:       1050    2379
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1797    2292    2814    3429 
   Size:       1797     495     522     615

  Fragments arranged by size: 

                1797     615     522     495
 
XmnI GAAnn'nnTTC

Cuts at:      0    3379    3429 
   Size:       3379      50
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I  HindIII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI
    PpuMI    PshAI   PspOMI     PstI    PvuII    SacII    SanDI     SapI
    SexAI     SfcI     SfiI     SfoI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AscI
     AseI    AsiSI    AvrII     BcgI     BclI    BglII     BplI    BsiWI
     BsmI    BspHI   BssHII    BstBI  BstZ17I     ClaI     DraI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KpnI     MfeI     NaeI     NcoI     NdeI   NgoMIV     NruI     PacI
     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SbfI     ScaI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XhoI     XmaI     ZraI
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