Restriction map for pF1KE3330
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AocI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BbeI        BbvCI       
BcgI        BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaI        BsmBI       BsmI        
BspDI       BspLU11I    Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        Eco47III    Eco81I      EcoRV       EcoT22I     
FbaI        FseI        HindIII     HpaI        KasI        
KpnI        MfeI        MluI        MunI        NarI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI          442  GGCGTC               G[GA]^CG[CT]C
AgeI         1445  ACCGGT               A^CCGGT
AhdI         2020  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2187  GAACGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2020  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         902  TGCGCA               TGC^GCA
BamHI         114  GGATCC               G^GATCC
BbrPI        1828  CACGTG               CAC^GTG
BfaI         1864  CTAG                 C^TAG
BglII        1889  AGATCT               A^GATCT
BsaBI         324  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         442  GGCGTC               G[GA]^CG[CT]C
BsiWI        1149  CGTACG               C^GTACG
Bsp1407I     1214  TGTACA               T^GTACA
BspHI        2171  TCATGA               T^CATGA
BsrGI        1214  TGTACA               T^GTACA
BssHII        721  GCGCGC               G^CGCGC
Eam1105I     2020  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1906  CTCTTC               CTCTTC(1/4)
EcoRI        1687  GAATTC               G^AATTC
FspI          902  TGCGCA               TGC^GCA
Hin1I         442  GGCGTC               G[GA]^CG[CT]C
HincII       2461  GTCAAC               GT[CT]^[GA]AC
HindII       2461  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1906  CTCTTC               CTCTTC(1/4)
MaeI         1864  CTAG                 C^TAG
MamI          324  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         1370  TTAA                 T^TAA
PinAI        1445  ACCGGT               A^CCGGT
PmaCI        1828  CACGTG               CAC^GTG
PmlI         1828  CACGTG               CAC^GTG
RcaI         2171  TCATGA               T^CATGA
SphI          899  GCATGC               GCATG^C
SspBI        1214  TGTACA               T^GTACA
Tru9I        1370  TTAA                 T^TAA
XmnI         2187  GAACGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          645  GTCTAC               GT^[AC][GT]AC
             1459  GTCTAC              
AccIII        332  TCCGGA               T^CCGGA
             2114  TCCGGA              
AcsI          186  GAATTT               [GA]^AATT[CT]
             1687  GAATTC              
Alw44I        545  GTGCAC               G^TGCAC
             1777  GTGCAC              
ApaLI         545  GTGCAC               G^TGCAC
             1777  GTGCAC              
ApoI          186  GAATTT               [GA]^AATT[CT]
             1687  GAATTC              
BsaAI        1828  CACGTG               [CT]AC^GT[GA]
             2356  TACGTG              
BseAI         332  TCCGGA               T^CCGGA
             2114  TCCGGA              
BsmAI        1190  GAGAC                GTCTC(1/5)
             2764  GAGAC               
BspEI         332  TCCGGA               T^CCGGA
             2114  TCCGGA              
BsrBI         813  CCGCTC               CCGCTC(-3/-3)
              823  CCGCTC              
BsrDI        1856  GCAATG               GCAATG(2/0)
             2336  GCAATG              
BssSI         505  CACGAG               CACGAG(-5/-1)
             1176  CTCGTG              
BstAPI       2124  GCAGGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2544  GCAGCAGGTGC         
BstEII       1468  GGTCACC              G^GT[ACGT]ACC
             1626  GGTGACC             
BstPI        1468  GGTCACC              G^GT[ACGT]ACC
             1626  GGTGACC             
BstXI         504  CCACGAGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              569  CCATTTATGTGG        
EcoNI        1548  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2043  CCTGTGGGAGG         
EcoO65I      1468  GGTCACC              G^GT[ACGT]ACC
             1626  GGTGACC             
KspI           22  CCGCGG               CCGC^GG
              316  CCGCGG              
MroI          332  TCCGGA               T^CCGGA
             2114  TCCGGA              
NcoI         2429  CCATGG               C^CATGG
             2645  CCATGG              
NotI           27  GCGGCCGC             GC^GGCCGC
               58  GCGGCCGC            
NspI          899  GCATGC               [GA]CATG^[CT]
             1277  GCATGT              
PleI          580  GACTC                GAGTC(4/5)
             2014  GACTC               
SacII          22  CCGCGG               CCGC^GG
              316  CCGCGG              
SfiI         1977  GGCCCCAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2484  GGCCTATGGGGCC       
TaqI         2012  TCGA                 T^CGA
             2177  TCGA                
TthHB8I      2012  TCGA                 T^CGA
             2177  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EagI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StyI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XcmI        XhoII       XmaCI       XmaI        
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