Restriction map for pF1KE3330
 (Linear) (Six-base) MAPSORT of: KIEE3330.seq  Check: 8928  from: 1  to: 2829



KIEE3330.orf

 With 182 enzymes: SgfI * 

                        March 2, 2011 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2115    2538    2787    2829 
   Size:       2115     423     249      42

  Fragments arranged by size: 

                2115     423     249      42
 
AccI GT'mk_AC

Cuts at:      0     646    1460    2829 
   Size:        646     814    1369

  Fragments arranged by size: 

                1369     814     646
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     571     826    1546    1641    2040    2829 
   Size:         77     494     255     720      95     399     789

  Fragments arranged by size: 

                 789     720     494     399     255      95      77
 
AgeI A'CCGG_T

Cuts at:      0    1445    2829 
   Size:       1445    1384
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2025    2829 
   Size:       2025     804
 
AleI CACnn'nnGTG

Cuts at:      0    2588    2829 
   Size:       2588     241
 
AlwNI CAG_nnn'CTG

Cuts at:      0     372     388    1644    1664    2130    2767    2829 
   Size:        372      16    1256      20     466     637      62

  Fragments arranged by size: 

                1256     637     466     372      62      20      16
 
ApaI G_GGCC'C

Cuts at:      0    1023    1123    1547    2366    2715    2829 
   Size:       1023     100     424     819     349     114

  Fragments arranged by size: 

                1023     819     424     349     114     100
 
ApaLI G'TGCA_C

Cuts at:      0     545    1777    2829 
   Size:        545    1232    1052

  Fragments arranged by size: 

                1232    1052     545
 
ApoI r'AATT_y

Cuts at:      0     186    1687    2829 
   Size:        186    1501    1142

  Fragments arranged by size: 

                1501    1142     186
 
AvaI C'yCGr_G

Cuts at:      0       5     728    1187    1488    2283    2829 
   Size:          5     723     459     301     795     546

  Fragments arranged by size: 

                 795     723     546     459     301       5
 
BamHI G'GATC_C

Cuts at:      0     114    2829 
   Size:        114    2715
 
BanI G'GyrC_C

Cuts at:      0     732    2433    2478    2550    2829 
   Size:        732    1701      45      72     279

  Fragments arranged by size: 

                1701     732     279      72      45
 
BanII G_rGCy'C

Cuts at:      0      13     883    1023    1123    1547    1766    2366    2715 
   Size:         13     870     140     100     424     219     600     349

Cuts at:   2715    2829 
   Size:        114

  Fragments arranged by size: 

                 870     600     424     349     219     140     114     100
                  13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     343     430    2046    2829 
   Size:        343      87    1616     783

  Fragments arranged by size: 

                1616     783     343      87
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55    1619    1984    2491    2829 
   Size:         55    1564     365     507     338

  Fragments arranged by size: 

                1564     507     365     338      55
 
BglII A'GATC_T

Cuts at:      0    1889    2829 
   Size:       1889     940
 
Bme1580I G_kGCm'C

Cuts at:      0     549     735    1023    1085    1123    1547    1781    2366 
   Size:        549     186     288      62      38     424     234     585

Cuts at:   2366    2438    2715    2829 
   Size:         72     277     114

  Fragments arranged by size: 

                 585     549     424     288     277     234     186     114
                  72      62      38
 
BmgBI CAC'GTC

Cuts at:      0     644    1719    2829 
   Size:        644    1075    1110

  Fragments arranged by size: 

                1110    1075     644
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2323    2391    2829 
   Size:       2323      68     438

  Fragments arranged by size: 

                2323     438      68
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2769    2829 
   Size:       2769      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     796    1780    2829 
   Size:        214     582     984    1049

  Fragments arranged by size: 

                1049     984     582     214
 
Bpu10I CC'TnA_GC

Cuts at:      0     957    1067    1645    2317    2829 
   Size:        957     110     578     672     512

  Fragments arranged by size: 

                 957     672     578     512     110
 
BsaAI yAC'GTr

Cuts at:      0    1830    2358    2829 
   Size:       1830     528     471

  Fragments arranged by size: 

                1830     528     471
 
BsaBI GATnn'nnATC

Cuts at:      0     328    2829 
   Size:        328    2501
 
BsaHI Gr'CG_yC

Cuts at:      0     443    2829 
   Size:        443    2386
 
BsaWI w'CCGG_w

Cuts at:      0     332     563    1445    2114    2829 
   Size:        332     231     882     669     715

  Fragments arranged by size: 

                 882     715     669     332     231
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     709     739    2829 
   Size:        709      30    2090

  Fragments arranged by size: 

                2090     709      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1444    1570    2546    2752    2755    2758    2829 
   Size:       1444     126     976     206       3       3      71

  Fragments arranged by size: 

                1444     976     206     126      71       3       3
 
BseYI C'CCAG_C

Cuts at:      0     459     915     927    1228    1342    1434    1617    1944 
   Size:        459     456      12     301     114      92     183     327

Cuts at:   1944    2197    2575    2775    2829 
   Size:        253     378     200      54

  Fragments arranged by size: 

                 459     456     378     327     301     253     200     183
                 114      92      54      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     328    2539    2746    2829 
   Size:        226     102    2211     207      83

  Fragments arranged by size: 

                2211     226     207     102      83
 
BsiEI CG_ry'CG

Cuts at:      0      31      50      62    2829 
   Size:         31      19      12    2767

  Fragments arranged by size: 

                2767      31      19      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     549     670    1587    1781    2323    2722    2829 
   Size:        549     121     917     194     542     399     107

  Fragments arranged by size: 

                 917     549     542     399     194     121     107
 
BsiWI C'GTAC_G

Cuts at:      0    1149    2829 
   Size:       1149    1680
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     549     670     735     883    1023    1085    1123 
   Size:         13     536     121      65     148     140      62      38

Cuts at:   1123    1547    1587    1766    1781    2323    2366    2438    2715 
   Size:        424      40     179      15     542      43      72     277

Cuts at:   2715    2722    2829 
   Size:          7     107

  Fragments arranged by size: 

                 542     536     424     277     179     148     140     121
                 107      72      65      62      43      40      38      15
                  13       7
 
BspEI T'CCGG_A

Cuts at:      0     332    2114    2829 
   Size:        332    1782     715

  Fragments arranged by size: 

                1782     715     332
 
BspHI T'CATG_A

Cuts at:      0    2171    2829 
   Size:       2171     658
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2115    2507    2538    2787    2829 
   Size:       2115     392      31     249      42

  Fragments arranged by size: 

                2115     392     249      42      31
 
BsrBI CCG'CTC

Cuts at:      0     815     825    2829 
   Size:        815      10    2004

  Fragments arranged by size: 

                2004     815      10
 
BsrDI GCAATG_nn'

Cuts at:      0    1863    2343    2829 
   Size:       1863     480     486

  Fragments arranged by size: 

                1863     486     480
 
BsrFI r'CCGG_y

Cuts at:      0    1445    2120    2201    2231    2475    2481    2829 
   Size:       1445     675      81      30     244       6     348

  Fragments arranged by size: 

                1445     675     348     244      81      30       6
 
BsrGI T'GTAC_A

Cuts at:      0    1214    2829 
   Size:       1214    1615
 
BssHII G'CGCG_C

Cuts at:      0     721    2829 
   Size:        721    2108
 
BssSI C'ACGA_G

Cuts at:      0     505    1176    2829 
   Size:        505     671    1653

  Fragments arranged by size: 

                1653     671     505
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2130    2550    2829 
   Size:       2130     420     279

  Fragments arranged by size: 

                2130     420     279
 
BstEII G'GTnAC_C

Cuts at:      0    1468    1626    2829 
   Size:       1468     158    1203

  Fragments arranged by size: 

                1468    1203     158
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     511     576    2829 
   Size:        511      65    2253

  Fragments arranged by size: 

                2253     511      65
 
BstYI r'GATC_y

Cuts at:      0     114    1889    2522    2829 
   Size:        114    1775     633     307

  Fragments arranged by size: 

                1775     633     307     114
 
BtgI C'CryG_G

Cuts at:      0      22     316    1015    2429    2624    2645    2829 
   Size:         22     294     699    1414     195      21     184

  Fragments arranged by size: 

                1414     699     294     195     184      22      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     792    1218    1236    2829 
   Size:        792     426      18    1593

  Fragments arranged by size: 

                1593     792     426      18
 
BtsI GCAGTG_nn'

Cuts at:      0     243    2601    2829 
   Size:        243    2358     228

  Fragments arranged by size: 

                2358     243     228
 
DraIII CAC_nnn'GTG

Cuts at:      0     702    1785    1830    2829 
   Size:        702    1083      45     999

  Fragments arranged by size: 

                1083     999     702      45
 
EaeI y'GGCC_r

Cuts at:      0      28      47      59     407    1511    1847    2829 
   Size:         28      19      12     348    1104     336     982

  Fragments arranged by size: 

                1104     982     348     336      28      19      12
 
EagI C'GGCC_G

Cuts at:      0      28      47      59    2829 
   Size:         28      19      12    2770

  Fragments arranged by size: 

                2770      28      19      12
 
EarI CTCTTCn'nnn_

Cuts at:      0    1912    2829 
   Size:       1912     917
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     336    1743    2829 
   Size:        336    1407    1086

  Fragments arranged by size: 

                1407    1086     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     214     571     796     826    1546    1641    1780 
   Size:         77     137     357     225      30     720      95     139

Cuts at:   1780    2040    2829 
   Size:        260     789

  Fragments arranged by size: 

                 789     720     357     260     225     139     137      95
                  77      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1552    2047    2829 
   Size:       1552     495     782

  Fragments arranged by size: 

                1552     782     495
 
EcoO109I rG'GnC_Cy

Cuts at:      0     133     369    1020    1119    1120    1543    1544    1842 
   Size:        133     236     651      99       1     423       1     298

Cuts at:   1842    2362    2376    2492    2711    2829 
   Size:        520      14     116     219     118

  Fragments arranged by size: 

                 651     520     423     298     236     219     133     118
                 116      99      14       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1687    2829 
   Size:       1687    1142
 
FspI TGC'GCA

Cuts at:      0     904    2829 
   Size:        904    1925
 
FspAI rTGC'GCAy

Cuts at:      0     904    2829 
   Size:        904    1925
 
HaeII r_GCGC'y

Cuts at:      0      19     865    2681    2829 
   Size:         19     846    1816     148

  Fragments arranged by size: 

                1816     846     148      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     150     182     347     379     652     684     709     741 
   Size:        150      32     165      32     273      32      25      32

Cuts at:    741    1159    1191    1801    1833    2011    2043    2829 
   Size:        418      32     610      32     178      32     786

  Fragments arranged by size: 

                 786     610     418     273     178     165     150      32
                  32      32      32      32      32      32      25
 
HincII GTy'rAC

Cuts at:      0    2463    2829 
   Size:       2463     366
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     490     638     842    2114    2282    2660    2829 
   Size:        187     303     148     204    1272     168     378     169

  Fragments arranged by size: 

                1272     378     303     204     187     169     168     148
            
 
MscI TGG'CCA

Cuts at:      0     409    1513    1849    2829 
   Size:        409    1104     336     980

  Fragments arranged by size: 

                1104     980     409     336
 
MslI CAynn'nnrTG

Cuts at:      0     509     574     832    1357    2407    2588    2644    2829 
   Size:        509      65     258     525    1050     181      56     185

  Fragments arranged by size: 

                1050     525     509     258     185     181      65      56
            
 
MspA1I CmG'CkG

Cuts at:      0      24      33     318     622     846    1320    1329    1970 
   Size:         24       9     285     304     224     474       9     641

Cuts at:   1970    2820    2829 
   Size:        850       9

  Fragments arranged by size: 

                 850     641     474     304     285     224      24       9
                   9       9
 
NaeI GCC'GGC

Cuts at:      0    2203    2233    2483    2829 
   Size:       2203      30     250     346

  Fragments arranged by size: 

                2203     346     250      30
 
NcoI C'CATG_G

Cuts at:      0    2429    2645    2829 
   Size:       2429     216     184

  Fragments arranged by size: 

                2429     216     184
 
NgoMIV G'CCGG_C

Cuts at:      0    2201    2231    2481    2829 
   Size:       2201      30     250     348

  Fragments arranged by size: 

                2201     348     250      30
 
NotI GC'GGCC_GC

Cuts at:      0      28      59    2829 
   Size:         28      31    2770

  Fragments arranged by size: 

                2770      31      28
 
NspI r_CATG'y

Cuts at:      0     903    1281    2829 
   Size:        903     378    1548

  Fragments arranged by size: 

                1548     903     378
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372     405    1353    1509    1830    2100    2707    2829 
   Size:        372      33     948     156     321     270     607     122

  Fragments arranged by size: 

                 948     607     372     321     270     156     122      33
            
 
PfoI T'CCnGG_A

Cuts at:      0      85     365    2829 
   Size:         85     280    2464

  Fragments arranged by size: 

                2464     280      85
 
PmlI CAC'GTG

Cuts at:      0    1830    2829 
   Size:       1830     999
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     499     531    2829 
   Size:        499      32    2298

  Fragments arranged by size: 

                2298     499      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     133     369    1842    2829 
   Size:        133     236    1473     987

  Fragments arranged by size: 

                1473     987     236     133
 
PspOMI G'GGCC_C

Cuts at:      0    1019    1119    1543    2362    2711    2829 
   Size:       1019     100     424     819     349     118

  Fragments arranged by size: 

                1019     819     424     349     118     100
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1987    2019    2829 
   Size:       1987      32     810

  Fragments arranged by size: 

                1987     810      32
 
PstI C_TGCA'G

Cuts at:      0    1028    2072    2798    2829 
   Size:       1028    1044     726      31

  Fragments arranged by size: 

                1044    1028     726      31
 
PvuII CAG'CTG

Cuts at:      0     846    1329    2820    2829 
   Size:        846     483    1491       9

  Fragments arranged by size: 

                1491     846     483       9
 
SacII CC_GC'GG

Cuts at:      0      25     319    2829 
   Size:         25     294    2510

  Fragments arranged by size: 

                2510     294      25
 
SfcI C'TryA_G

Cuts at:      0    1024    2068    2794    2829 
   Size:       1024    1044     726      35

  Fragments arranged by size: 

                1044    1024     726      35
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1984    2491    2829 
   Size:       1984     507     338

  Fragments arranged by size: 

                1984     507     338
 
SmaI CCC'GGG

Cuts at:      0       7     730    1490    2829 
   Size:          7     723     760    1339

  Fragments arranged by size: 

                1339     760     723       7
 
SphI G_CATG'C

Cuts at:      0     903    2829 
   Size:        903    1926
 
StyI C'CwwG_G

Cuts at:      0     222    1631    2429    2645    2829 
   Size:        222    1409     798     216     184

  Fragments arranged by size: 

                1409     798     222     216     184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     750    2148    2829 
   Size:        750    1398     681

  Fragments arranged by size: 

                1398     750     681
 
TatI w'GTAC_w

Cuts at:      0     800    1214    2829 
   Size:        800     414    1615

  Fragments arranged by size: 

                1615     800     414
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      81     113    1033    1065    2829 
   Size:         81      32     920      32    1764

  Fragments arranged by size: 

                1764     920      81      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1350    1827    2097    2593    2643    2829 
   Size:       1350     477     270     496      50     186

  Fragments arranged by size: 

                1350     496     477     270     186      50
 
XmaI C'CCGG_G

Cuts at:      0       5     728    1488    2829 
   Size:          5     723     760    1341

  Fragments arranged by size: 

                1341     760     723       5
 
XmnI GAAnn'nnTTC

Cuts at:      0    2191    2829 
   Size:       2191     638
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AhdI     AleI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BglI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NotI     NspI    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI
     PsrI     PstI    PvuII    SacII     SfcI     SfiI     SmaI     SphI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AlfI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI     BmtI    BpuEI     BsaI     BsmI
    BsmBI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
  EcoICRI    EcoRV     FalI     FseI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NruI     NsiI     PacI
     PasI     PciI     PmeI    PshAI     PsiI    PspXI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     ZraI
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