Restriction map for pF1KE2301
##################################
# NO CUT ENZYMES (   41 enzymes) #
##################################

AatII       AscI        AseI        AsnI        AvrII       
BamHI       BbeI        BglI        BlnI        BsiWI       
BsmBI       BssHII      BstBI       BstEII      BstPI       
DrdI        EcoO65I     FseI        HindIII     HpaI        
KasI        MfeI        MluI        MunI        NarI        
NdeI        NheI        NruI        NspV        PacI        
PmeI        PshBI       PvuI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SwaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       5881  GGTACC               G^GTACC
AccIII       5451  TCCGGA               T^CCGGA
AclI         3881  AACGTT               AA^CGTT
AgeI         2879  ACCGGT               A^CCGGT
Asp718I      5881  GGTACC               G^GTACC
AspI         4185  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3848  TGCGCA               TGC^GCA
BalI          716  TGGCCA               TGG^CCA
BbsI         4074  GTCTTC               GAAGAC(2/6)
BcgI         4850  CGAAGAAGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4097  TGATCA               T^GATCA
BpuAI        4074  GTCTTC               GAAGAC(2/6)
BsaBI        4689  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        5451  TCCGGA               T^CCGGA
BspDI        4972  ATCGAT               AT^CGAT
BspEI        5451  TCCGGA               T^CCGGA
Bst1107I     1839  GTATAC               GTA^TAC
BstZ17I      1839  GTATAC               GTA^TAC
ClaI         4972  ATCGAT               AT^CGAT
CpoI         2881  CGGTCCG              CG^G[AT]CCG
DraI         2952  TTTAAA               TTT^AAA
EagI           46  CGGCCG               C^GGCCG
EclXI          46  CGGCCG               C^GGCCG
Eco52I         46  CGGCCG               C^GGCCG
EcoRI        1774  GAATTC               G^AATTC
EcoT22I      4585  ATGCAT               ATGCA^T
FbaI         4097  TGATCA               T^GATCA
FspI         3848  TGCGCA               TGC^GCA
KpnI         5881  GGTACC               GGTAC^C
KspI           49  CCGCGG               CCGC^GG
MamI         4689  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         716  TGGCCA               TGG^CCA
MroI         5451  TCCGGA               T^CCGGA
MscI          716  TGGCCA               TGG^CCA
NaeI          884  GCCGGC               GCC^GGC
NcoI         5885  CCATGG               C^CATGG
NgoMIV        884  GCCGGC               G^CCGGC
NotI           45  GCGGCCGC             GC^GGCCGC
NsiI         4585  ATGCAT               ATGCA^T
PaeR7I       5144  CTCGAG               C^TCGAG
PflFI        4185  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2879  ACCGGT               A^CCGGT
Psp1406I     3881  AACGTT               AA^CGTT
RsrII        2881  CGGTCCG              CG^G[AT]CCG
SacII          49  CCGCGG               CCGC^GG
SalI         4708  GTCGAC               G^TCGAC
SmaI         5415  CCCGGG               CCC^GGG
SspI         2642  AATATT               AAT^ATT
Tth111I      4185  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         5144  CTCGAG               C^TCGAG
XmaCI        5415  CCCGGG               C^CCGGG
XmaI         5415  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflII        3286  CTTAAG               C^TTAAG
             3889  CTTAAG              
AhdI         3813  GACAGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4711  GACATGCAGTC         
Alw44I       4519  GTGCAC               G^TGCAC
             5995  GTGCAC              
AocI           76  CCTCAGG              CC^T[ACGT]AGG
             5187  CCTGAGG             
Aor51HI      2903  AGCGCT               AGC^GCT
             4262  AGCGCT              
ApaLI        4519  GTGCAC               G^TGCAC
             5995  GTGCAC              
AspEI        3813  GACAGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4711  GACATGCAGTC         
BbrPI        2191  CACGTG               CAC^GTG
             4947  CACGTG              
BfrI         3286  CTTAAG               C^TTAAG
             3889  CTTAAG              
BsaWI        2879  ACCGGT               [AT]^CCGG[AT]
             5451  TCCGGA              
BsiEI          46  CGGCCG               CG[GA][CT]^CG
             3609  CGACCG              
BsmI          740  GAATGC               GAATGC(1/-1)
             4037  GCATTC              
BssSI        2568  CACGAG               CACGAG(-5/-1)
             5558  CTCGTG              
Bsu36I         76  CCTCAGG              CC^T[ACGT]AGG
             5187  CCTGAGG             
DraIII       3260  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4662  CACGGAGTG           
Eam1105I     3813  GACAGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4711  GACATGCAGTC         
Eco47III     2903  AGCGCT               AGC^GCT
             4262  AGCGCT              
Eco81I         76  CCTCAGG              CC^T[ACGT]AGG
             5187  CCTGAGG             
EcoNI        2814  CCTCAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3583  CCTACAGTAGG         
EcoRV        2329  GATATC               GAT^ATC
             2482  GATATC              
PmaCI        2191  CACGTG               CAC^GTG
             4947  CACGTG              
PmlI         2191  CACGTG               CAC^GTG
             4947  CACGTG              
PshAI         262  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5646  GACTGGCGTC          
SacI         2971  GAGCTC               GAGCT^C
             4729  GAGCTC              
SexAI        2525  ACCAGGT              A^CC[AT]GGT
             4613  ACCTGGT             
StuI         3290  AGGCCT               AGG^CCT
             5517  AGGCCT              
XbaI         3075  TCTAGA               T^CTAGA
             4257  TCTAGA              
XcmI         4673  CCATGCTCCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5466  CCACCCAAACCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApaI        ApoI        Asp700I     AspHI       AvaI        
AvaII       BanI        BanII       BbvCI       BbvI        
BciVI       BcnI        BfaI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SfaNI       SfcI        SmlI        Sse8387I    SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI        
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