Restriction map for pF1KB5948
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApaI        AscI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MaeII       MamI        MluI        MroI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI         1629  CCGC                 CCGC(-3/-1)
Alw44I        437  GTGCAC               G^TGCAC
AocI         1291  CCTGAGG              CC^T[ACGT]AGG
ApaLI         437  GTGCAC               G^TGCAC
AvaI         1183  CTCGGG               C^[CT]CG[GA]G
BalI          881  TGGCCA               TGG^CCA
BanI         1287  GGCACC               G^G[CT][GA]CC
BanII        1441  GAGCTC               G[GA]GC[CT]^C
BciVI         445  GGATAC               GTATCC(6/5)
BsgI         1132  CTGCAC               GTGCAG(16/14)
BsmFI         785  GGGAC                GGGAC(10/14)
BsoBI        1183  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      698  TGTACA               T^GTACA
BspHI         947  TCATGA               T^CATGA
BsrGI         698  TGTACA               T^GTACA
BstAPI       1103  GCAGAGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         283  CCAGAGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1291  CCTGAGG              CC^T[ACGT]AGG
CfoI          198  GCGC                 GCG^C
DraII         785  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          107  CCATGG               C^C[GA][CT]GG
EaeI          881  TGGCCA               [CT]^GGCC[GA]
Eco81I       1291  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      785  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV        1330  GATATC               GAT^ATC
HaeII         197  AGCGCC               [GA]GCGC^[CT]
HhaI          198  GCGC                 GCG^C
HinP1I        198  GCGC                 G^CGC
HincII        394  GTCAAC               GT[CT]^[GA]AC
HindII        394  GTCAAC               GT[CT]^[GA]AC
MfeI         1355  CAATTG               C^AATTG
MluNI         881  TGGCCA               TGG^CCA
MscI          881  TGGCCA               TGG^CCA
MslI         1260  CACTATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1355  CAATTG               C^AATTG
NcoI          107  CCATGG               C^CATGG
PpuMI         785  GGGACCC              [GA]G^G[AT]CC[CT]
PstI         1101  CTGCAG               CTGCA^G
RcaI          947  TCATGA               T^CATGA
SacI         1441  GAGCTC               GAGCT^C
ScaI          691  AGTACT               AGT^ACT
SexAI        1142  ACCAGGT              A^CC[AT]GGT
SfcI         1101  CTGCAG               C^T[GA][CT]AG
SmlI         1498  CTTGAG               C^T[CT][GA]AG
SspBI         698  TGTACA               T^GTACA
XbaI          357  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwI         1149  GATCC                GGATC(4/5)
             1522  GGATC               
AlwNI         479  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              836  CAGGTACTG           
AseI          924  ATTAAT               AT^TAAT
              931  ATTAAT              
AsnI          924  ATTAAT               AT^TAAT
              931  ATTAAT              
AspHI         437  GTGCAC               G[AT]GC[AT]^C
             1441  GAGCTC              
AvaII         786  GGACC                G^G[AT]CC
             1318  GGTCC               
BfaI          358  CTAG                 C^TAG
              856  CTAG                
BglII         206  AGATCT               A^GATCT
              749  AGATCT              
BsaI         1066  GAGACC               GGTCTC(1/5)
             1337  GGTCTC              
BsiHKAI       437  GTGCAC               G[AT]GC[AT]^C
             1441  GAGCTC              
BsiYI         157  CCAGGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1274  CCCCATATTGG         
BslI          157  CCAGGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1274  CCCCATATTGG         
Cac8I        1112  GCCTGC               GC[ACGT]^[ACGT]GC
             1116  GCAAGC              
DraI         1485  TTTAAA               TTT^AAA
             1554  TTTAAA              
EcoRI         268  GAATTC               G^AATTC
              278  GAATTC              
EcoT14I       107  CCATGG               C^C[AT][AT]GG
             1529  CCAAGG              
HindIII      1118  AAGCTT               A^AGCTT
             1310  AAGCTT              
MaeI          358  CTAG                 C^TAG
              856  CTAG                
MspA1I       1094  CAGCTG               C[AC]G^C[GT]G
             1466  CAGCTG              
PleI         1302  GACTC                GAGTC(4/5)
             1584  GACTC               
PshBI         924  ATTAAT               AT^TAAT
              931  ATTAAT              
PvuII        1094  CAGCTG               CAG^CTG
             1466  CAGCTG              
SspI          970  AATATT               AAT^ATT
             1174  AATATT              
StyI          107  CCATGG               C^C[AT][AT]GG
             1529  CCAAGG              
TaqI          466  TCGA                 T^CGA
             1305  TCGA                
TspRI          44  CACTG                CA[GC]TG[ACGT][ACGT]^
              273  CACTG               
TthHB8I       466  TCGA                 T^CGA
             1305  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsmAI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com