Restriction map for
pF1KB5948
(Circular) (Six-base) MAPSORT of: pF1KB5948.seq Check: 2771 from: 1 to: 4745
pF1KB5948 4745 bp
With 182 enzymes: SgfI *
November 17, 2014 16:43 ..
AccI GT'mk_AC
Cuts at: 1783 1783
Size: 4745
Acc65I G'GTAC_C
Cuts at: 1761 1761
Size: 4745
AclI AA'CG_TT
Cuts at: 4585 4585
Size: 4745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 245 311 641 2570 3002 3749 4017 167
Size: 78 66 330 1929 432 747 268 895
Fragments arranged by size:
1929 895 747 432 330 268 78 66
AflIII A'CryG_T
Cuts at: 2097 3201 4178 2097
Size: 1104 977 2664
Fragments arranged by size:
2664 1104 977
AgeI A'CCGG_T
Cuts at: 3133 3133
Size: 4745
AleI CACnn'nnGTG
Cuts at: 1372 1372
Size: 4745
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1224 1224
Size: 4745
AlwNI CAG_nnn'CTG
Cuts at: 592 949 2050 3617 592
Size: 357 1101 1567 1720
Fragments arranged by size:
1720 1567 1101 357
ApaLI G'TGCA_C
Cuts at: 545 3515 545
Size: 2970 1775
ApoI r'AATT_y
Cuts at: 376 386 478 1046 1749 4098 4354 376
Size: 10 92 568 703 2349 256 767
Fragments arranged by size:
2349 767 703 568 256 92 10
AseI AT'TA_AT
Cuts at: 20 1033 1040 20
Size: 1013 7 3725
Fragments arranged by size:
3725 1013 7
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
AvaI C'yCGr_G
Cuts at: 1291 1765 1291
Size: 474 4271
BamHI G'GATC_C
Cuts at: 1770 1770
Size: 4745
BanI G'GyrC_C
Cuts at: 1395 1761 2175 2423 2458 1395
Size: 366 414 248 35 3682
Fragments arranged by size:
3682 414 366 248 35
BanII G_rGCy'C
Cuts at: 1553 1759 2789 1553
Size: 206 1030 3509
Fragments arranged by size:
3509 1030 206
BbeI G_GCGC'C
Cuts at: 2179 2427 2179
Size: 248 4497
BciVI GTATCCnnnnn_n'
Cuts at: 547 2643 3404 4745 547
Size: 2096 761 1341 547
Fragments arranged by size:
2096 1341 761 547
BclI T'GATC_A
Cuts at: 2265 2265
Size: 4745
BglII A'GATC_T
Cuts at: 314 857 2260 4297 314
Size: 543 1403 2037 762
Fragments arranged by size:
2037 1403 762 543
BlpI GC'TnA_GC
Cuts at: 1857 1857
Size: 4745
Bme1580I G_kGCm'C
Cuts at: 405 549 1338 2370 2463 3519 405
Size: 144 789 1032 93 1056 1631
Fragments arranged by size:
1631 1056 1032 789 144 93
BmrI ACTGGGnnnn_n'
Cuts at: 1957 2038 2373 4517 1957
Size: 81 335 2144 2185
Fragments arranged by size:
2185 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 4745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1529 1537 1668 1529
Size: 8 131 4606
Fragments arranged by size:
4606 131 8
Bpu10I CC'TnA_GC
Cuts at: 1349 1349
Size: 4745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 1921 2800 3292 3590 1627
Size: 294 879 492 298 2782
Fragments arranged by size:
2782 879 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 1168 1451 4449 1168
Size: 283 2998 1464
Fragments arranged by size:
2998 1464 283
BsaAI yAC'GTr
Cuts at: 2098 2098
Size: 4745
BsaBI GATnn'nnATC
Cuts at: 6 2259 4296 6
Size: 2253 2037 455
Fragments arranged by size:
2253 2037 455
BsaHI Gr'CG_yC
Cuts at: 2176 2424 3126 4622 2176
Size: 248 702 1496 2299
Fragments arranged by size:
2299 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1934 2455 3133 3407 3554 3965 1934
Size: 521 678 274 147 411 2714
Fragments arranged by size:
2714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 395 425 184
Size: 30 181 30 4504
Fragments arranged by size:
4504 181 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 137 284 868 1685 137
Size: 147 584 817 3197
Fragments arranged by size:
3197 817 584 147
BseYI C'CCAG_C
Cuts at: 2170 2928 3505 4001 2170
Size: 758 577 496 2914
Fragments arranged by size:
2914 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 1225
Size: 4745
BsiEI CG_ry'CG
Cuts at: 104 2287 2333 3541 104
Size: 2183 46 1208 1308
Fragments arranged by size:
2183 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 549 1553 1759 2537 2727 3519 549
Size: 1004 206 778 190 792 1775
Fragments arranged by size:
1775 1004 792 778 206 190
Bsp1286I G_dGCh'C
Cuts at: 405 549 1338 1553 1759 2370 2463 2537 2727
Size: 144 789 215 206 611 93 74 190
Cuts at: 2727 2789 3519 405
Size: 62 730 1631
Fragments arranged by size:
1631 789 730 611 215 206 190 144
93 74 62
BspEI T'CCGG_A
Cuts at: 3965 3965
Size: 4745
BspHI T'CATG_A
Cuts at: 1055 3921 1055
Size: 2866 1879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1795 2009 2311 2692 1795
Size: 214 302 381 3848
Fragments arranged by size:
3848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3037 3091 4575 4740 50
Size: 2987 54 1484 165 55
Fragments arranged by size:
2987 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 403 734 968 2657 403
Size: 331 234 1689 2491
Fragments arranged by size:
2491 1689 331 234
BsrFI r'CCGG_y
Cuts at: 2743 2924 3133 2743
Size: 181 209 4355
Fragments arranged by size:
4355 209 181
BsrGI T'GTAC_A
Cuts at: 806 806
Size: 4745
BssSI C'ACGA_G
Cuts at: 3016 3374 3016
Size: 358 4387
BstAPI GCAn_nnn'nTGC
Cuts at: 1217 2122 1217
Size: 905 3840
BstBI TT'CG_AA
Cuts at: 3106 3106
Size: 4745
BstXI CCAn_nnnn'nTGG
Cuts at: 398 398
Size: 4745
BstYI r'GATC_y
Cuts at: 1 314 857 1630 1770 2237 2260 2595 2841
Size: 313 543 773 140 467 23 335 246
Cuts at: 2841 3853 3939 3951 4297 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 773 543 467 449 346 335 313
246 140 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1400 1400
Size: 4745
BtgI C'CryG_G
Cuts at: 215 215
Size: 4745
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2695 2876 3039 2695
Size: 181 163 4401
Fragments arranged by size:
4401 181 163
BtsI GCAGTG_nn'
Cuts at: 1988 1988
Size: 4745
DraI TTT'AAA
Cuts at: 1595 1664 1744 1595
Size: 69 80 4596
Fragments arranged by size:
4596 80 69
DrdI GACnn_nn'nnGTC
Cuts at: 2451 3309 2451
Size: 858 3887
EaeI y'GGCC_r
Cuts at: 989 2330 2504 2895 2922 4267 989
Size: 1341 174 391 27 1345 1467
Fragments arranged by size:
1467 1345 1341 391 174 27
EagI C'GGCC_G
Cuts at: 2330 2330
Size: 4745
EarI CTCTTCn'nnn_
Cuts at: 125 221 326 629 2768 2978 125
Size: 96 105 303 2139 210 1892
Fragments arranged by size:
2139 1892 303 210 105 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2953 3263 3409 4555 2953
Size: 310 146 1146 3143
Fragments arranged by size:
3143 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1551 1757 1551
Size: 206 4539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 245 311 641 1529 1537 1668 2570 3002
Size: 78 66 330 888 8 131 902 432
Cuts at: 3002 3749 4017 167
Size: 747 268 895
Fragments arranged by size:
902 895 888 747 432 330 268 131
78 66 8
EcoO109I rG'GnC_Cy
Cuts at: 894 1884 894
Size: 990 3755
EcoRI G'AATT_C
Cuts at: 376 386 1749 376
Size: 10 1363 3372
Fragments arranged by size:
3372 1363 10
EcoRV GAT'ATC
Cuts at: 1440 1440
Size: 4745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 265 2764 265
Size: 2499 2246
FspI TGC'GCA
Cuts at: 2526 2526
Size: 4745
HaeII r_GCGC'y
Cuts at: 309 2179 2427 3449 4428 309
Size: 1870 248 1022 979 626
Fragments arranged by size:
1870 1022 979 626 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1429 1461 1555 1587 2587 2617 2619 2649 1429
Size: 32 94 32 1000 30 2 30 3525
Fragments arranged by size:
3525 1000 94 32 32 30 30 2
HincII GTy'rAC
Cuts at: 504 1784 504
Size: 1280 3465
HindIII A'AGCT_T
Cuts at: 1226 1418 1226
Size: 192 4553
KasI G'GCGC_C
Cuts at: 2175 2423 2175
Size: 248 4497
KpnI G_GTAC'C
Cuts at: 1765 1765
Size: 4745
MfeI C'AATT_G
Cuts at: 1463 1463
Size: 4745
MluI A'CGCG_T
Cuts at: 4178 4178
Size: 4745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 3416 3600 446
Size: 2970 184 1591
Fragments arranged by size:
2970 1591 184
MscI TGG'CCA
Cuts at: 991 2506 991
Size: 1515 3230
MslI CAynn'nnrTG
Cuts at: 1372 2734 2861 1372
Size: 1362 127 3256
Fragments arranged by size:
3256 1362 127
MspA1I CmG'CkG
Cuts at: 1204 1576 1856 2170 2530 3543 3788 1204
Size: 372 280 314 360 1013 245 2161
Fragments arranged by size:
2161 1013 372 360 314 280 245
NaeI GCC'GGC
Cuts at: 2926 2926
Size: 4745
NarI GG'CG_CC
Cuts at: 2176 2424 2176
Size: 248 4497
NcoI C'CATG_G
Cuts at: 215 215
Size: 4745
NgoMIV G'CCGG_C
Cuts at: 2924 2924
Size: 4745
NspI r_CATG'y
Cuts at: 1798 2298 3205 1798
Size: 500 907 3338
Fragments arranged by size:
3338 907 500
PciI A'CATG_T
Cuts at: 3201 3201
Size: 4745
PmeI GTTT'AAAC
Cuts at: 1744 1744
Size: 4745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4713
PpuMI rG'GwC_Cy
Cuts at: 894 894
Size: 4745
PstI C_TGCA'G
Cuts at: 1213 1792 2477 1213
Size: 579 685 3481
Fragments arranged by size:
3481 685 579
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4745
PvuII CAG'CTG
Cuts at: 1204 1576 2170 2530 1204
Size: 372 594 360 3419
Fragments arranged by size:
3419 594 372 360
RsrII CG'GwC_CG
Cuts at: 2940 2940
Size: 4745
SacI G_AGCT'C
Cuts at: 1553 1759 1553
Size: 206 4539
SalI G'TCGA_C
Cuts at: 1782 1782
Size: 4745
SanDI GG'GwC_CC
Cuts at: 894 894
Size: 4745
SapI GCTCTTCn'nnn_
Cuts at: 2768 2978 2768
Size: 210 4535
SbfI CC_TGCA'GG
Cuts at: 1792 1792
Size: 4745
ScaI AGT'ACT
Cuts at: 801 4262 801
Size: 3461 1284
SexAI A'CCwGG_T
Cuts at: 1250 1250
Size: 4745
SfcI C'TryA_G
Cuts at: 33 1209 1788 2473 3466 3657 33
Size: 1176 579 685 993 191 1121
Fragments arranged by size:
1176 1121 993 685 579 191
SfoI GGC'GCC
Cuts at: 2177 2425 2177
Size: 248 4497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
SmaI CCC'GGG
Cuts at: 1767 1767
Size: 4745
SmlI C'TyrA_G
Cuts at: 1606 1900 2815 3307 3569 1606
Size: 294 915 492 262 2782
Fragments arranged by size:
2782 915 492 294 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4745
SphI G_CATG'C
Cuts at: 1798 2298 1798
Size: 500 4245
SspI AAT'ATT
Cuts at: 1080 1284 1080
Size: 204 4541
StyI C'CwwG_G
Cuts at: 215 1637 1879 2233 215
Size: 1422 242 354 2727
Fragments arranged by size:
2727 1422 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1099 2328 2461 2920 3129 1099
Size: 1229 133 459 209 2715
Fragments arranged by size:
2715 1229 459 209 133
TatI w'GTAC_w
Cuts at: 577 799 806 4260 577
Size: 222 7 3454 1062
Fragments arranged by size:
3454 1062 222 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 372 395 404 427 971 1003 4262 4294 372
Size: 23 9 23 544 32 3259 32 823
Fragments arranged by size:
3259 823 544 32 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 2542 2542
Size: 4745
XbaI T'CTAG_A
Cuts at: 465 1776 465
Size: 1311 3434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2240 2240
Size: 4745
XmaI C'CCGG_G
Cuts at: 1765 1765
Size: 4745
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI