Restriction map for
pF1KE2343
(Circular) (Six-base) MAPSORT of: pF1KE2343.seq Check: 8767 from: 1 to: 5072
pF1KE2343 5072 bp
With 182 enzymes: SgfI *
May 18, 2011 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1629 1629
Size: 5072
AatII G_ACGT'C
Cuts at: 170 170
Size: 5072
AccI GT'mk_AC
Cuts at: 1118 2110 1118
Size: 992 4080
Acc65I G'GTAC_C
Cuts at: 602 2088 602
Size: 1486 3586
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 2897 3329 4076 4344 922
Size: 1975 432 747 268 1650
Fragments arranged by size:
1975 1650 747 432 268
AfeI AGC'GCT
Cuts at: 292 1424 292
Size: 1132 3940
AflIII A'CryG_T
Cuts at: 950 2064 2424 3528 4505 950
Size: 1114 360 1104 977 1517
Fragments arranged by size:
1517 1114 1104 977 360
AgeI A'CCGG_T
Cuts at: 3460 3460
Size: 5072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1441 1441
Size: 5072
AlwNI CAG_nnn'CTG
Cuts at: 162 1806 2377 3944 162
Size: 1644 571 1567 1290
Fragments arranged by size:
1644 1567 1290 571
ApaI G_GGCC'C
Cuts at: 492 562 701 929 1164 1230 1268 2002 492
Size: 70 139 228 235 66 38 734 3562
Fragments arranged by size:
3562 734 235 228 139 70 66 38
ApaLI G'TGCA_C
Cuts at: 3842 3842
Size: 5072
ApoI r'AATT_y
Cuts at: 2076 4425 4681 2076
Size: 2349 256 2467
Fragments arranged by size:
2467 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 394 574 804 928 1362 1604 1941 2092 394
Size: 180 230 124 434 242 337 151 3374
Fragments arranged by size:
3374 434 337 242 230 180 151 124
AvrII C'CTAG_G
Cuts at: 2002 2002
Size: 5072
BamHI G'GATC_C
Cuts at: 390 2097 390
Size: 1707 3365
BanI G'GyrC_C
Cuts at: 379 570 602 609 634 840 880 1456 1501
Size: 191 32 7 25 206 40 576 45
Cuts at: 1501 1641 1705 2088 2502 2750 2785 379
Size: 140 64 383 414 248 35 2666
Fragments arranged by size:
2666 576 414 383 248 206 191 140
64 45 40 35 32 25 7
BanII G_rGCy'C
Cuts at: 401 492 502 562 701 929 962 1111 1139
Size: 91 10 60 139 228 33 149 28
Cuts at: 1139 1164 1230 1268 2002 2086 3116 401
Size: 25 66 38 734 84 1030 2357
Fragments arranged by size:
2357 1030 734 228 149 139 91 84
66 60 38 33 28 25 10
BbeI G_GCGC'C
Cuts at: 383 574 613 638 1460 1505 2506 2754 383
Size: 191 39 25 822 45 1001 248 2701
Fragments arranged by size:
2701 1001 822 248 191 45 39 25
BbvCI CC'TCA_GC
Cuts at: 702 702
Size: 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5038
BciVI GTATCCnnnnn_n'
Cuts at: 2970 3731 5072 2970
Size: 761 1341 2970
Fragments arranged by size:
2970 1341 761
BclI T'GATC_A
Cuts at: 2592 2592
Size: 5072
BglI GCCn_nnn'nGGC
Cuts at: 976 1478 1540 976
Size: 502 62 4508
Fragments arranged by size:
4508 502 62
BglII A'GATC_T
Cuts at: 2587 4624 2587
Size: 2037 3035
BlpI GC'TnA_GC
Cuts at: 2184 2184
Size: 5072
Bme1580I G_kGCm'C
Cuts at: 249 492 562 701 929 1164 1230 1268 1708
Size: 243 70 139 228 235 66 38 440
Cuts at: 1708 1876 2002 2697 2790 3846 249
Size: 168 126 695 93 1056 1475
Fragments arranged by size:
1475 1056 695 440 243 235 228 168
139 126 93 70 66 38
BmrI ACTGGGnnnn_n'
Cuts at: 499 2284 2365 2700 4844 499
Size: 1785 81 335 2144 727
Fragments arranged by size:
2144 1785 727 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 324 3439 324
Size: 3115 1957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1252 1339 1510 1996 1153
Size: 99 87 171 486 4229
Fragments arranged by size:
4229 486 171 99 87
Bpu10I CC'TnA_GC
Cuts at: 702 1345 702
Size: 643 4429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1291 2248 3127 3619 3917 1081
Size: 210 957 879 492 298 2236
Fragments arranged by size:
2236 957 879 492 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 4776 4776
Size: 5072
BsaAI yAC'GTr
Cuts at: 1770 2425 1770
Size: 655 4417
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 167 362 380 571 610 635 1066 1088 1457
Size: 195 18 191 39 25 431 22 369
Cuts at: 1457 1502 2503 2751 3453 4949 167
Size: 45 1001 248 702 1496 290
Fragments arranged by size:
1496 1001 702 431 369 290 248 195
191 45 39 25 22 18
BsaWI w'CCGG_w
Cuts at: 2059 2261 2782 3460 3734 3881 4292 2059
Size: 202 521 678 274 147 411 2839
Fragments arranged by size:
2839 678 521 411 274 202 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 973 1003 1051 1081 1318 1348 157
Size: 30 786 30 48 30 237 30 3881
Fragments arranged by size:
3881 786 237 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 1075 1160 1336 1339 1513 1561 162
Size: 913 85 176 3 174 48 3673
Fragments arranged by size:
3673 913 176 174 85 48 3
BseYI C'CCAG_C
Cuts at: 131 1428 1452 1823 1899 2497 3255 3832 4328
Size: 1297 24 371 76 598 758 577 496
Cuts at: 4328 131
Size: 875
Fragments arranged by size:
1297 875 758 598 577 496 371 76
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 1888 1294
Size: 594 4478
BsiEI CG_ry'CG
Cuts at: 104 587 662 872 1544 1928 2614 2660 3868
Size: 483 75 210 672 384 686 46 1208
Cuts at: 3868 104
Size: 1308
Fragments arranged by size:
1308 1208 686 672 483 384 210 75
46
BsiHKAI G_wGCw'C
Cuts at: 2086 2864 3054 3846 2086
Size: 778 190 792 3312
Fragments arranged by size:
3312 792 778 190
BsiWI C'GTAC_G
Cuts at: 953 953
Size: 5072
BsmBI CGTCTCn'nnnn_
Cuts at: 174 174
Size: 5072
Bsp1286I G_dGCh'C
Cuts at: 249 401 492 502 562 701 929 962 1111
Size: 152 91 10 60 139 228 33 149
Cuts at: 1111 1139 1164 1230 1268 1708 1876 2002 2086
Size: 28 25 66 38 440 168 126 84
Cuts at: 2086 2697 2790 2864 3054 3116 3846 249
Size: 611 93 74 190 62 730 1475
Fragments arranged by size:
1475 730 611 440 228 190 168 152
149 139 126 93 91 84 74 66
62 60 38 33 28 25 10
BspEI T'CCGG_A
Cuts at: 2059 4292 2059
Size: 2233 2839
BspHI T'CATG_A
Cuts at: 4248 4248
Size: 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1629 2122 2336 2638 3019 1629
Size: 493 214 302 381 3682
Fragments arranged by size:
3682 493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1158 1647 1848 3364 3418 4902 5067 50
Size: 1108 489 201 1516 54 1484 165 55
Fragments arranged by size:
1516 1484 1108 489 201 165 55 54
BsrDI GCAATG_nn'
Cuts at: 552 2984 552
Size: 2432 2640
BsrFI r'CCGG_y
Cuts at: 189 541 586 837 1745 3070 3251 3460 189
Size: 352 45 251 908 1325 181 209 1801
Fragments arranged by size:
1801 1325 908 352 251 209 181 45
BsrGI T'GTAC_A
Cuts at: 713 713
Size: 5072
BssHII G'CGCG_C
Cuts at: 623 758 1301 1514 1516 1546 1631 1835 623
Size: 135 543 213 2 30 85 204 3860
Fragments arranged by size:
3860 543 213 204 135 85 30 2
BssSI C'ACGA_G
Cuts at: 1172 3343 3701 1172
Size: 2171 358 2543
Fragments arranged by size:
2543 2171 358
BstAPI GCAn_nnn'nTGC
Cuts at: 551 1806 2449 551
Size: 1255 643 3174
Fragments arranged by size:
3174 1255 643
BstBI TT'CG_AA
Cuts at: 3433 3433
Size: 5072
BstXI CCAn_nnnn'nTGG
Cuts at: 1215 1452 2022 1215
Size: 237 570 4265
Fragments arranged by size:
4265 570 237
BstYI r'GATC_y
Cuts at: 1 390 1802 2097 2564 2587 2922 3168 4180
Size: 389 1412 295 467 23 335 246 1012
Cuts at: 4180 4266 4278 4624 1
Size: 86 12 346 449
Fragments arranged by size:
1412 1012 467 449 389 346 335 295
246 86 23 12
BtgI C'CryG_G
Cuts at: 866 974 1001 1055 1381 1405 1721 1794 866
Size: 108 27 54 326 24 316 73 4144
Fragments arranged by size:
4144 326 316 108 73 54 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3022 3203 3366 3022
Size: 181 163 4728
Fragments arranged by size:
4728 181 163
BtsI GCAGTG_nn'
Cuts at: 269 1282 2315 269
Size: 1013 1033 3026
Fragments arranged by size:
3026 1033 1013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 223 258 1062 1097 223
Size: 35 804 35 4198
Fragments arranged by size:
4198 804 35 35
DraI TTT'AAA
Cuts at: 2071 2071
Size: 5072
DrdI GACnn_nn'nnGTC
Cuts at: 2778 3636 2778
Size: 858 4214
EaeI y'GGCC_r
Cuts at: 148 403 584 659 869 1541 1925 1988 2657
Size: 255 181 75 210 672 384 63 669
Cuts at: 2657 2831 3222 3249 4594 148
Size: 174 391 27 1345 626
Fragments arranged by size:
1345 672 669 626 391 384 255 210
181 174 75 63 27
EagI C'GGCC_G
Cuts at: 584 659 869 1541 1925 2657 584
Size: 75 210 672 384 732 2999
Fragments arranged by size:
2999 732 672 384 210 75
EarI CTCTTCn'nnn_
Cuts at: 3095 3305 3095
Size: 210 4862
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1297 3280 3590 3736 4882 1297
Size: 1983 310 146 1146 1487
Fragments arranged by size:
1983 1487 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2084 2084
Size: 5072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1153 1252 1339 1510 1996 2897 3329 4076
Size: 231 99 87 171 486 901 432 747
Cuts at: 4076 4344 922
Size: 268 1650
Fragments arranged by size:
1650 901 747 486 432 268 231 171
99 87
EcoNI CCTnn'n_nnAGG
Cuts at: 1525 1525
Size: 5072
EcoO109I rG'GnC_Cy
Cuts at: 488 489 558 698 925 969 1101 1161 1226
Size: 1 69 140 227 44 132 60 65
Cuts at: 1226 1227 1265 1971 1999 2006 2211 488
Size: 1 38 706 28 7 205 3349
Fragments arranged by size:
3349 706 227 205 140 132 69 65
60 44 38 28 7 1 1
EcoRI G'AATT_C
Cuts at: 2076 2076
Size: 5072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 5072
FspI TGC'GCA
Cuts at: 2853 2853
Size: 5072
HaeII r_GCGC'y
Cuts at: 294 383 574 613 638 709 1318 1426 1460
Size: 89 191 39 25 71 609 108 34
Cuts at: 1460 1505 1594 1752 1777 1819 1864 1956 1987
Size: 45 89 158 25 42 45 92 31
Cuts at: 1987 2506 2754 3776 4755 294
Size: 519 248 1022 979 611
Fragments arranged by size:
1022 979 611 609 519 248 191 158
108 92 89 89 71 45 45 42
39 34 31 25 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 244 276 973 1005 1051 1083 2914
Size: 32 55 32 697 32 46 32 1831
Cuts at: 2914 2944 2946 2976 157
Size: 30 2 30 2253
Fragments arranged by size:
2253 1831 697 55 46 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2111 2111
Size: 5072
KasI G'GCGC_C
Cuts at: 379 570 609 634 1456 1501 2502 2750 379
Size: 191 39 25 822 45 1001 248 2701
Fragments arranged by size:
2701 1001 822 248 191 45 39 25
KpnI G_GTAC'C
Cuts at: 606 2092 606
Size: 1486 3586
MluI A'CGCG_T
Cuts at: 950 4505 950
Size: 3555 1517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 302 3743 3927 182
Size: 120 3441 184 1327
Fragments arranged by size:
3441 1327 184 120
MscI TGG'CCA
Cuts at: 150 405 1990 2833 150
Size: 255 1585 843 2389
Fragments arranged by size:
2389 1585 843 255
MslI CAynn'nnrTG
Cuts at: 3061 3188 3061
Size: 127 4945
MspA1I CmG'CkG
Cuts at: 711 1003 1057 1383 1407 1436 1452 1723 1796
Size: 292 54 326 24 29 16 271 73
Cuts at: 1796 1812 1908 2183 2497 2857 3870 4115 711
Size: 16 96 275 314 360 1013 245 1668
Fragments arranged by size:
1668 1013 360 326 314 292 275 271
245 96 73 54 29 24 16 16
NaeI GCC'GGC
Cuts at: 191 543 588 839 1747 3253 191
Size: 352 45 251 908 1506 2010
Fragments arranged by size:
2010 1506 908 352 251 45
NarI GG'CG_CC
Cuts at: 380 571 610 635 1457 1502 2503 2751 380
Size: 191 39 25 822 45 1001 248 2701
Fragments arranged by size:
2701 1001 822 248 191 45 39 25
NgoMIV G'CCGG_C
Cuts at: 189 541 586 837 1745 3251 189
Size: 352 45 251 908 1506 2010
Fragments arranged by size:
2010 1506 908 352 251 45
NotI GC'GGCC_GC
Cuts at: 1541 1541
Size: 5072
NspI r_CATG'y
Cuts at: 2125 2625 3532 2125
Size: 500 907 3665
Fragments arranged by size:
3665 907 500
PasI CC'CwG_GG
Cuts at: 214 345 492 1222 214
Size: 131 147 730 4064
Fragments arranged by size:
4064 730 147 131
PciI A'CATG_T
Cuts at: 3528 3528
Size: 5072
PflMI CCAn_nnn'nTGG
Cuts at: 113 201 113
Size: 88 4984
PfoI T'CCnGG_A
Cuts at: 1151 1151
Size: 5072
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 5040
PpuMI rG'GwC_Cy
Cuts at: 1101 1971 1101
Size: 870 4202
PspOMI G'GGCC_C
Cuts at: 488 558 697 925 1160 1226 1264 1998 488
Size: 70 139 228 235 66 38 734 3562
Fragments arranged by size:
3562 734 235 228 139 70 66 38
PstI C_TGCA'G
Cuts at: 674 1640 1811 1952 2119 2804 674
Size: 966 171 141 167 685 2942
Fragments arranged by size:
2942 966 685 171 167 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5072
PvuII CAG'CTG
Cuts at: 1452 1908 2497 2857 1452
Size: 456 589 360 3667
Fragments arranged by size:
3667 589 456 360
RsrII CG'GwC_CG
Cuts at: 1385 3267 1385
Size: 1882 3190
SacI G_AGCT'C
Cuts at: 2086 2086
Size: 5072
SacII CC_GC'GG
Cuts at: 1004 1058 1384 1408 1724 1797 1004
Size: 54 326 24 316 73 4279
Fragments arranged by size:
4279 326 316 73 54 24
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SapI GCTCTTCn'nnn_
Cuts at: 3095 3305 3095
Size: 210 4862
SbfI CC_TGCA'GG
Cuts at: 2119 2119
Size: 5072
ScaI AGT'ACT
Cuts at: 307 4589 307
Size: 4282 790
SfcI C'TryA_G
Cuts at: 33 670 1636 1807 1948 2115 2800 3793 3984
Size: 637 966 171 141 167 685 993 191
Cuts at: 3984 33
Size: 1121
Fragments arranged by size:
1121 993 966 685 637 191 171 167
141
SfoI GGC'GCC
Cuts at: 381 572 611 636 1458 1503 2504 2752 381
Size: 191 39 25 822 45 1001 248 2701
Fragments arranged by size:
2701 1001 822 248 191 45 39 25
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SgrAI Cr'CCGG_yG
Cuts at: 1745 1745
Size: 5072
SmaI CCC'GGG
Cuts at: 806 1364 1606 1943 2094 806
Size: 558 242 337 151 3784
Fragments arranged by size:
3784 558 337 242 151
SmlI C'TyrA_G
Cuts at: 1096 1306 2227 3142 3634 3896 1096
Size: 210 921 915 492 262 2272
Fragments arranged by size:
2272 921 915 492 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5072
SphI G_CATG'C
Cuts at: 2125 2625 2125
Size: 500 4572
StuI AGG'CCT
Cuts at: 449 449
Size: 5072
StyI C'CwwG_G
Cuts at: 428 965 1991 2002 2015 2206 2560 428
Size: 537 1026 11 13 191 354 2940
Fragments arranged by size:
2940 1026 537 354 191 13 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2655 2788 3247 3456 2655
Size: 133 459 209 4271
Fragments arranged by size:
4271 459 209 133
TatI w'GTAC_w
Cuts at: 305 713 4587 305
Size: 408 3874 790
Fragments arranged by size:
3874 790 408
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1318 1350 4589 4621 1318
Size: 32 3239 32 1769
Fragments arranged by size:
3239 1769 32 32
Tth111I GACn'n_nGTC
Cuts at: 2869 2869
Size: 5072
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 972 2567 972
Size: 1595 3477
XmaI C'CCGG_G
Cuts at: 804 1362 1604 1941 2092 804
Size: 558 242 337 151 3784
Fragments arranged by size:
3784 558 337 242 151
ZraI GAC'GTC
Cuts at: 168 168
Size: 5072
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI BaeI BbsI BfrBI
BmgBI BmtI BsmI BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XhoI XmnI