Restriction map for pF1KE1600
##################################
# NO CUT ENZYMES (   41 enzymes) #
##################################

AgeI        Asp700I     AspI        AviII       AvrII       
BbrPI       BcgI        BlnI        BsaBI       BsiWI       
Bst1107I    BstBI       BstZ17I     DraI        DraIII      
FseI        FspI        HpaI        MamI        MluI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
SbfI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        Tth111I     XbaI        
XmnI        


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# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        5581  GACGTC               GACGT^C
AccIII       3889  TCCGGA               T^CCGGA
AclI         3334  AACGTT               AA^CGTT
AflII        5493  CTTAAG               C^TTAAG
Alw44I       3652  GTGCAC               G^TGCAC
AocI         4846  CCTGAGG              CC^T[ACGT]AGG
ApaLI        3652  GTGCAC               G^TGCAC
AscI          262  GGCGCGCC             GG^CGCGCC
AseI         4918  ATTAAT               AT^TAAT
AsnI         4918  ATTAAT               AT^TAAT
BfrI         5493  CTTAAG               C^TTAAG
BsaAI        4899  TACGTG               [CT]AC^GT[GA]
BseAI        3889  TCCGGA               T^CCGGA
BspDI        5575  ATCGAT               AT^CGAT
BspEI        3889  TCCGGA               T^CCGGA
BspHI        4467  TCATGA               T^CATGA
Bsu36I       4846  CCTGAGG              CC^T[ACGT]AGG
ClaI         5575  ATCGAT               AT^CGAT
Eco81I       4846  CCTGAGG              CC^T[ACGT]AGG
HindIII      6426  AAGCTT               A^AGCTT
MfeI         1502  CAATTG               C^AATTG
MroI         3889  TCCGGA               T^CCGGA
MunI         1502  CAATTG               C^AATTG
NdeI         4069  CATATG               CA^TATG
PaeR7I       5995  CTCGAG               C^TCGAG
PshBI        4918  ATTAAT               AT^TAAT
Psp1406I     3334  AACGTT               AA^CGTT
RcaI         4467  TCATGA               T^CATGA
SalI         3163  GTCGAC               G^TCGAC
SapI         5542  GAAGAGC              GCTCTTC(1/4)
ScaI         5000  AGTACT               AGT^ACT
StuI         5853  AGGCCT               AGG^CCT
XhoI         5995  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         3163  GTCGAC               GT^[AC][GT]AC
             6520  GTAGAC              
BamHI        1380  GGATCC               G^GATCC
             3505  GGATCC              
BclI          582  TGATCA               T^GATCA
             5079  TGATCA              
BglII        1741  AGATCT               A^GATCT
             1824  AGATCT              
BsaWI        3889  TCCGGA               [AT]^CCGG[AT]
             5684  ACCGGA              
BsmBI           4  GAGACG               CGTCTC(1/5)
             5723  GAGACG              
BsmI         1541  GAATGC               GAATGC(1/-1)
             2480  GAATGC              
BspMI        2969  ACCTGC               ACCTGC(4/8)
             5503  GCAGGT              
BssHII        263  GCGCGC               G^CGCGC
             1157  GCGCGC              
BstEII       3568  GGTGACC              G^GT[ACGT]ACC
             4719  GGTCACC             
BstPI        3568  GGTGACC              G^GT[ACGT]ACC
             4719  GGTCACC             
CpoI         1344  CGGACCG              CG^G[AT]CCG
             4187  CGGACCG             
DrdI         3525  GACCCCCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4569  GACGCCACCGTC        
EcoO65I      3568  GGTGACC              G^GT[ACGT]ACC
             4719  GGTCACC             
EcoRI        5033  GAATTC               G^AATTC
             6586  GAATTC              
FbaI          582  TGATCA               T^GATCA
             5079  TGATCA              
NheI         3004  GCTAGC               G^CTAGC
             6552  GCTAGC              
PpuMI        3782  AGGACCC              [GA]G^G[AT]CC[CT]
             6156  GGGACCC             
RsrII        1344  CGGACCG              CG^G[AT]CCG
             4187  CGGACCG             
SspI         4921  AATATT               AAT^ATT
             5794  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  164 enzymes) #
#########################################

Acc65I      AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AhdI        AluI        AlwI        
AlwNI       Aor51HI     ApaI        ApoI        Asp718I     
AspEI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BciVI       BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NotI        
NsiI        NspI        PflMI       PleI        PstI        
PvuII       RsaI        SacI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
SfoI        SmaI        SmlI        SphI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI        
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