Restriction map for
pF1KE1600
(Circular) (Six-base) MAPSORT of: pF1KE1600.seq Check: 4077 from: 1 to: 9806
pF1KE1600 9806 bp
With 182 enzymes: SgfI *
July 13, 2010 13:08 ..
AatII G_ACGT'C
Cuts at: 5693 5693
Size: 9806
AccI GT'mk_AC
Cuts at: 3272 6629 6844 3272
Size: 3357 215 6234
Fragments arranged by size:
6234 3357 215
Acc65I G'GTAC_C
Cuts at: 800 1805 4554 6822 800
Size: 1005 2749 2268 3784
Fragments arranged by size:
3784 2749 2268 1005
AclI AA'CG_TT
Cuts at: 3443 9646 3443
Size: 6203 3603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3537 3703 5669 5741 7631 8063 8810 9078 3537
Size: 166 1966 72 1890 432 747 268 4265
Fragments arranged by size:
4265 1966 1890 747 432 268 166 72
AfeI AGC'GCT
Cuts at: 642 1144 4735 642
Size: 502 3591 5713
Fragments arranged by size:
5713 3591 502
AflII C'TTAA_G
Cuts at: 5601 5601
Size: 9806
AflIII A'CryG_T
Cuts at: 1823 3977 4223 6122 7158 8262 9239 1823
Size: 2154 246 1899 1036 1104 977 2390
Fragments arranged by size:
2390 2154 1899 1104 1036 977 246
AgeI A'CCGG_T
Cuts at: 8194 8194
Size: 9806
AhdI GACnn_n'nnGTC
Cuts at: 2414 3364 5217 2414
Size: 950 1853 7003
Fragments arranged by size:
7003 1853 950
AleI CACnn'nnGTG
Cuts at: 3229 3229
Size: 9806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2768 3865 4480 6205 2768
Size: 1097 615 1725 6369
Fragments arranged by size:
6369 1725 1097 615
AlwNI CAG_nnn'CTG
Cuts at: 1782 2923 3498 7111 8678 1782
Size: 1141 575 3613 1567 2910
Fragments arranged by size:
3613 2910 1567 1141 575
ApaI G_GGCC'C
Cuts at: 460 762 1078 2098 2510 4294 4357 4495 5741
Size: 302 316 1020 412 1784 63 138 1246
Cuts at: 5741 460
Size: 4525
Fragments arranged by size:
4525 1784 1246 1020 412 316 302 138
63
ApaLI G'TGCA_C
Cuts at: 3760 8576 3760
Size: 4816 4990
ApoI r'AATT_y
Cuts at: 5141 5570 6382 6694 6810 9159 9415 5141
Size: 429 812 312 116 2349 256 5532
Fragments arranged by size:
5532 2349 812 429 312 256 116
AscI GG'CGCG_CC
Cuts at: 371 371
Size: 9806
AseI AT'TA_AT
Cuts at: 20 5027 20
Size: 5007 4799
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9806
AvaI C'yCGr_G
Cuts at: 453 540 586 715 1078 1246 1633 2088 2200
Size: 87 46 129 363 168 387 455 112
Cuts at: 2200 3013 3609 4110 4287 4356 6103 6826 453
Size: 813 596 501 177 69 1747 723 3433
Fragments arranged by size:
3433 1747 813 723 596 501 455 387
363 177 168 129 112 87 69 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1588 1621 1588
Size: 33 9773
BamHI G'GATC_C
Cuts at: 1488 3613 6831 1488
Size: 2125 3218 4463
Fragments arranged by size:
4463 3218 2125
BanI G'GyrC_C
Cuts at: 415 550 800 1372 1444 1805 2478 2874 2892
Size: 135 250 572 72 361 673 396 18
Cuts at: 2892 3448 4133 4512 4554 4710 5585 6822 7236
Size: 556 685 379 42 156 875 1237 414
Cuts at: 7236 7484 7519 415
Size: 248 35 2702
Fragments arranged by size:
2702 1237 875 685 673 572 556 414
396 379 361 250 248 156 135 72
42 35 18
BanII G_rGCy'C
Cuts at: 460 762 1015 1042 1078 1441 1987 2098 2510
Size: 302 253 27 36 363 546 111 412
Cuts at: 2510 3472 3539 3884 3935 4294 4357 4495 5741
Size: 962 67 345 51 359 63 138 1246
Cuts at: 5741 5822 6820 7850 460
Size: 81 998 1030 2416
Fragments arranged by size:
2416 1246 1030 998 962 546 412 363
359 345 302 253 138 111 81 67
63 51 36 27
BbeI G_GCGC'C
Cuts at: 419 554 1376 2878 2896 4516 4714 5589 7240
Size: 135 822 1502 18 1620 198 875 1651
Cuts at: 7240 7488 419
Size: 248 2737
Fragments arranged by size:
2737 1651 1620 1502 875 822 248 198
135 18
BbsI GAAGACnn'nnnn_
Cuts at: 1895 3543 5288 5309 5320 5729 1895
Size: 1648 1745 21 11 409 5972
Fragments arranged by size:
5972 1745 1648 409 21 11
BbvCI CC'TCA_GC
Cuts at: 377 1102 1550 1661 4433 4629 377
Size: 725 448 111 2772 196 5554
Fragments arranged by size:
5554 2772 725 448 196 111
BciVI GTATCCnnnnn_n'
Cuts at: 1616 2179 4100 4337 5782 6334 7704 8465 9806
Size: 563 1921 237 1445 552 1370 761 1341
Cuts at: 9806 1616
Size: 1616
Fragments arranged by size:
1921 1616 1445 1370 1341 761 563 552
237
BclI T'GATC_A
Cuts at: 690 5187 7326 690
Size: 4497 2139 3170
Fragments arranged by size:
4497 3170 2139
BfrBI ATG'CAT
Cuts at: 2065 2367 2541 5888 2065
Size: 302 174 3347 5983
Fragments arranged by size:
5983 3347 302 174
BglI GCCn_nnn'nGGC
Cuts at: 668 757 845 967 1082 1123 1396 1405 1592
Size: 89 88 122 115 41 273 9 187
Cuts at: 1592 1718 4217 4499 668
Size: 126 2499 282 5975
Fragments arranged by size:
5975 2499 282 273 187 126 122 115
89 88 41 9
BglII A'GATC_T
Cuts at: 1849 1932 7321 9358 1849
Size: 83 5389 2037 2297
Fragments arranged by size:
5389 2297 2037 83
BlpI GC'TnA_GC
Cuts at: 469 1036 1986 5879 6918 469
Size: 567 950 3893 1039 3357
Fragments arranged by size:
3893 3357 1039 950 567
Bme1580I G_kGCm'C
Cuts at: 460 562 587 762 1078 2098 2483 2510 2579
Size: 102 25 175 316 1020 385 27 69
Cuts at: 2579 3764 4136 4294 4357 4495 5741 6229 7431
Size: 1185 372 158 63 138 1246 488 1202
Cuts at: 7431 7524 8580 460
Size: 93 1056 1686
Fragments arranged by size:
1686 1246 1202 1185 1056 1020 488 385
372 316 175 158 138 102 93 69
63 27 25
BmgBI CAC'GTC
Cuts at: 4599 4599
Size: 9806
BmrI ACTGGGnnnn_n'
Cuts at: 1287 1371 1785 2477 2642 3221 3633 5070 6421
Size: 84 414 692 165 579 412 1437 1351
Cuts at: 6421 6558 7018 7099 7434 9578 1287
Size: 137 460 81 335 2144 1515
Fragments arranged by size:
2144 1515 1437 1351 692 579 460 414
412 335 165 137 84 81
BmtI G_CTAG'C
Cuts at: 3116 6664 3116
Size: 3548 6258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3321 3323 8173 3321
Size: 2 4850 4954
Fragments arranged by size:
4954 4850 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2064 2439 3571 4184 4404 4689 4762 5830
Size: 490 375 1132 613 220 285 73 1068
Cuts at: 5830 6322 1574
Size: 492 5058
Fragments arranged by size:
5058 1132 1068 613 492 490 375 285
220 73
Bpu10I CC'TnA_GC
Cuts at: 377 1102 1550 1661 4433 4629 377
Size: 725 448 111 2772 196 5554
Fragments arranged by size:
5554 2772 725 448 196 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2256 2385 3300 3438 6132 6982 7861 8353 8651
Size: 129 915 138 2694 850 879 492 298
Cuts at: 8651 2256
Size: 3411
Fragments arranged by size:
3411 2694 915 879 850 492 298 138
129
BsaI GGTCTCn'nnnn_
Cuts at: 1500 5159 6298 9510 1500
Size: 3659 1139 3212 1796
Fragments arranged by size:
3659 3212 1796 1139
BsaAI yAC'GTr
Cuts at: 5009 7159 5009
Size: 2150 7656
BsaBI GATnn'nnATC
Cuts at: 6 7320 9357 6
Size: 7314 2037 455
Fragments arranged by size:
7314 2037 455
BsaHI Gr'CG_yC
Cuts at: 416 551 1373 2875 2893 4513 4678 4711 5360
Size: 135 822 1502 18 1620 165 33 649
Cuts at: 5360 5586 5690 7237 7485 8187 9683 416
Size: 226 104 1547 248 702 1496 539
Fragments arranged by size:
1620 1547 1502 1496 822 702 649 539
248 226 165 135 104 33 18
BsaWI w'CCGG_w
Cuts at: 3997 5792 6995 7516 8194 8468 8615 9026 3997
Size: 1795 1203 521 678 274 147 411 4777
Fragments arranged by size:
4777 1795 1203 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 724 754 4863 4893 724
Size: 30 4109 30 5637
Fragments arranged by size:
5637 4109 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 893 896 899 911 914 917 920 923 926
Size: 3 3 12 3 3 3 3 3
Cuts at: 926 929 944 1850 3111 3189 3547 5260 5302
Size: 3 15 906 1261 78 358 1713 42
Cuts at: 5302 5311 5567 5831 6061 6350 893
Size: 9 256 264 230 289 4349
Fragments arranged by size:
4349 1713 1261 906 358 289 264 256
230 78 42 15 12 9 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 676 1210 1261 1796 2463 2599 3103 3240 3696
Size: 534 51 535 667 136 504 137 456
Cuts at: 3696 4714 4719 5170 7231 7989 8566 9062 676
Size: 1018 5 451 2061 758 577 496 1420
Fragments arranged by size:
2061 1420 1018 758 667 577 535 534
504 496 456 451 137 136 51 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3482 5488 5500 6196 6526 6660 3482
Size: 2006 12 696 330 134 6628
Fragments arranged by size:
6628 2006 696 330 134 12
BsiEI CG_ry'CG
Cuts at: 104 528 578 611 672 962 1115 1343 1409
Size: 424 50 33 61 290 153 228 66
Cuts at: 1409 3276 4062 7348 7394 8602 104
Size: 1867 786 3286 46 1208 1308
Fragments arranged by size:
3286 1867 1308 1208 786 424 290 228
153 66 61 50 46 33
BsiHKAI G_wGCw'C
Cuts at: 539 1042 1987 3472 3764 5540 6027 6820 7598
Size: 503 945 1485 292 1776 487 793 778
Cuts at: 7598 7788 8580 539
Size: 190 792 1765
Fragments arranged by size:
1776 1765 1485 945 793 792 778 503
487 292 190
BsmI GAATG_Cn'
Cuts at: 1655 2594 1655
Size: 939 8867
BsmBI CGTCTCn'nnnn_
Cuts at: 106 5825 106
Size: 5719 4087
Bsp1286I G_dGCh'C
Cuts at: 460 539 562 587 762 1015 1042 1078 1441
Size: 79 23 25 175 253 27 36 363
Cuts at: 1441 1987 2098 2483 2510 2579 3472 3539 3764
Size: 546 111 385 27 69 893 67 225
Cuts at: 3764 3884 3935 4136 4294 4357 4495 5540 5741
Size: 120 51 201 158 63 138 1045 201
Cuts at: 5741 5822 6027 6229 6820 7431 7524 7598 7788
Size: 81 205 202 591 611 93 74 190
Cuts at: 7788 7850 8580 460
Size: 62 730 1686
Fragments arranged by size:
1686 1045 893 730 611 591 546 385
363 253 225 205 202 201 201 190
175 158 138 120 111 93 81 79
74 69 67 63 62 51 36 27
27 25 23
BspEI T'CCGG_A
Cuts at: 3997 9026 3997
Size: 5029 4777
BspHI T'CATG_A
Cuts at: 4575 8982 4575
Size: 4407 5399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3086 5602 6856 7070 7372 7753 3086
Size: 2516 1254 214 302 381 5139
Fragments arranged by size:
5139 2516 1254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 656 940 6689 8098 8152 9636 9801 50
Size: 606 284 5749 1409 54 1484 165 55
Fragments arranged by size:
5749 1484 1409 606 284 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4010 4464 5713 5940 7718 4010
Size: 454 1249 227 1778 6098
Fragments arranged by size:
6098 1778 1249 454 227
BsrFI r'CCGG_y
Cuts at: 516 573 839 1117 1150 6713 7804 7985 8194
Size: 57 266 278 33 5563 1091 181 209
Cuts at: 8194 516
Size: 2128
Fragments arranged by size:
5563 2128 1091 278 266 209 181 57
33
BsrGI T'GTAC_A
Cuts at: 3980 4226 4262 3980
Size: 246 36 9524
Fragments arranged by size:
9524 246 36
BssHII G'CGCG_C
Cuts at: 371 1265 371
Size: 894 8912
BssSI C'ACGA_G
Cuts at: 2010 4213 6001 6706 8077 8435 2010
Size: 2203 1788 705 1371 358 3381
Fragments arranged by size:
3381 2203 1788 1371 705 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1671 1782 1996 7183 1671
Size: 111 214 5187 4294
Fragments arranged by size:
5187 4294 214 111
BstBI TT'CG_AA
Cuts at: 8167 8167
Size: 9806
BstEII G'GTnAC_C
Cuts at: 3676 4827 3676
Size: 1151 8655
BstXI CCAn_nnnn'nTGG
Cuts at: 2542 2566 3646 5083 2542
Size: 24 1080 1437 7265
Fragments arranged by size:
7265 1437 1080 24
BstYI r'GATC_y
Cuts at: 1 1468 1488 1849 1932 3613 4364 6246 6831
Size: 1467 20 361 83 1681 751 1882 585
Cuts at: 6831 7298 7321 7656 7902 8914 9000 9012 9358
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9358 1
Size: 449
Fragments arranged by size:
1882 1681 1467 1012 751 585 467 449
361 346 335 246 86 83 23 20
12
Bsu36I CC'TnA_GG
Cuts at: 4955 4955
Size: 9806
BtgI C'CryG_G
Cuts at: 107 962 1019 1343 1460 1640 1961 2900 3329
Size: 855 57 324 117 180 321 939 429
Cuts at: 3329 3659 4808 6446 6665 107
Size: 330 1149 1638 219 3248
Fragments arranged by size:
3248 1638 1149 939 855 429 330 324
321 219 180 117 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1447 5325 5339 5838 5898 7756 7937 8100 1447
Size: 3878 14 499 60 1858 181 163 3153
Fragments arranged by size:
3878 3153 1858 499 181 163 60 14
BtsI GCAGTG_nn'
Cuts at: 2744 3465 4687 7049 2744
Size: 721 1222 2362 5501
Fragments arranged by size:
5501 2362 1222 721
ClaI AT'CG_AT
Cuts at: 5684 5684
Size: 9806
DraI TTT'AAA
Cuts at: 6805 6805
Size: 9806
DrdI GACnn_nn'nnGTC
Cuts at: 3639 4683 7512 8370 3639
Size: 1044 2829 858 5075
Fragments arranged by size:
5075 2829 1044 858
EaeI y'GGCC_r
Cuts at: 438 525 608 669 959 1112 1152 1329 1340
Size: 87 83 61 290 153 40 177 11
Cuts at: 1340 1406 1643 3166 3714 5704 6137 6353 6647
Size: 66 237 1523 548 1990 433 216 294
Cuts at: 6647 6668 7391 7565 7956 7983 9328 438
Size: 21 723 174 391 27 1345 916
Fragments arranged by size:
1990 1523 1345 916 723 548 433 391
294 290 237 216 177 174 153 87
83 66 61 40 27 21 11
EagI C'GGCC_G
Cuts at: 525 608 669 959 1112 1340 1406 7391 525
Size: 83 61 290 153 228 66 5985 2940
Fragments arranged by size:
5985 2940 290 228 153 83 66 61
EarI CTCTTCn'nnn_
Cuts at: 1498 3290 5645 7829 8039 1498
Size: 1792 2355 2184 210 3265
Fragments arranged by size:
3265 2355 2184 1792 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 716 726 732 890 8014 8324 8470 9616 716
Size: 10 6 158 7124 310 146 1146 906
Fragments arranged by size:
7124 1146 906 310 158 146 10 6
EcoICRI GAG'CTC
Cuts at: 1040 1985 3470 6818 1040
Size: 945 1485 3348 4028
Fragments arranged by size:
4028 3348 1485 945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2064 2439 3537 3571 3703 4184 4404 4689
Size: 490 375 1098 34 132 481 220 285
Cuts at: 4689 4762 5669 5741 5830 6322 7631 8063 8810
Size: 73 907 72 89 492 1309 432 747
Cuts at: 8810 9078 1574
Size: 268 2302
Fragments arranged by size:
2302 1309 1098 907 747 492 490 481
432 375 285 268 220 132 89 73
72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 4335 4523 1105
Size: 3230 188 6388
Fragments arranged by size:
6388 3230 188
EcoO109I rG'GnC_Cy
Cuts at: 758 759 1075 2094 2506 3891 4102 4353 4429
Size: 1 316 1019 412 1385 211 251 76
Cuts at: 4429 4491 4492 5738 6265 6945 758
Size: 62 1 1246 527 680 3619
Fragments arranged by size:
3619 1385 1246 1019 680 527 412 316
251 211 76 62 1 1
EcoRI G'AATT_C
Cuts at: 5141 6694 6810 5141
Size: 1553 116 8137
Fragments arranged by size:
8137 1553 116
EcoRV GAT'ATC
Cuts at: 4944 6726 6734 4944
Size: 1782 8 8016
Fragments arranged by size:
8016 1782 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2426 3912 5781 7825 2426
Size: 1486 1869 2044 4407
Fragments arranged by size:
4407 2044 1869 1486
FspI TGC'GCA
Cuts at: 7587 7587
Size: 9806
HaeII r_GCGC'y
Cuts at: 419 554 644 1146 1376 2878 2896 4032 4212
Size: 135 90 502 230 1502 18 1136 180
Cuts at: 4212 4516 4714 4737 5589 6454 7240 7488 8510
Size: 304 198 23 852 865 786 248 1022
Cuts at: 8510 9489 419
Size: 979 736
Fragments arranged by size:
1502 1136 1022 979 865 852 786 736
502 304 248 230 198 180 135 90
23 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1176 1208 1662 1694 2400 2432 2929 2961 3007
Size: 32 454 32 706 32 497 32 46
Cuts at: 3007 3039 3350 3382 3757 3789 4557 4589 5203
Size: 32 311 32 375 32 768 32 614
Cuts at: 5203 5235 5680 5712 7648 7678 7680 7710 1176
Size: 32 445 32 1936 30 2 30 3272
Fragments arranged by size:
3272 1936 768 706 614 497 454 445
375 311 46 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3273 3644 5454 6845 3273
Size: 371 1810 1391 6234
Fragments arranged by size:
6234 1810 1391 371
HindIII A'AGCT_T
Cuts at: 6534 6534
Size: 9806
KasI G'GCGC_C
Cuts at: 415 550 1372 2874 2892 4512 4710 5585 7236
Size: 135 822 1502 18 1620 198 875 1651
Cuts at: 7236 7484 415
Size: 248 2737
Fragments arranged by size:
2737 1651 1620 1502 875 822 248 198
135 18
KpnI G_GTAC'C
Cuts at: 804 1809 4558 6826 804
Size: 1005 2749 2268 3784
Fragments arranged by size:
3784 2749 2268 1005
MfeI C'AATT_G
Cuts at: 1610 1610
Size: 9806
MluI A'CGCG_T
Cuts at: 9239 9239
Size: 9806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 1606 3381 6280 8477 8661 362
Size: 1244 1775 2899 2197 184 1507
Fragments arranged by size:
2899 2197 1775 1507 1244 184
MscI TGG'CCA
Cuts at: 3168 6139 6355 6670 7567 3168
Size: 2971 216 315 897 5407
Fragments arranged by size:
5407 2971 897 315 216
MslI CAynn'nnrTG
Cuts at: 197 218 1069 3134 3229 3289 3847 5893 6502
Size: 21 851 2065 95 60 558 2046 609
Cuts at: 6502 6595 7795 7922 197
Size: 93 1200 127 2081
Fragments arranged by size:
2081 2065 2046 1200 851 609 558 127
95 93 60 21
MspA1I CmG'CkG
Cuts at: 465 893 964 1021 1197 1345 1387 1602 2498
Size: 428 71 57 176 148 42 215 896
Cuts at: 2498 2599 4407 5365 6739 6917 7231 7591 8604
Size: 101 1808 958 1374 178 314 360 1013
Cuts at: 8604 8849 465
Size: 245 1422
Fragments arranged by size:
1808 1422 1374 1013 958 896 428 360
314 245 215 178 176 148 101 71
57 42
NaeI GCC'GGC
Cuts at: 518 841 1119 1152 7987 518
Size: 323 278 33 6835 2337
Fragments arranged by size:
6835 2337 323 278 33
NarI GG'CG_CC
Cuts at: 416 551 1373 2875 2893 4513 4711 5586 7237
Size: 135 822 1502 18 1620 198 875 1651
Cuts at: 7237 7485 416
Size: 248 2737
Fragments arranged by size:
2737 1651 1620 1502 875 822 248 198
135 18
NcoI C'CATG_G
Cuts at: 107 1460 1640 2900 3659 6446 6665 107
Size: 1353 180 1260 759 2787 219 3248
Fragments arranged by size:
3248 2787 1353 1260 759 219 180
NdeI CA'TA_TG
Cuts at: 4178 4178
Size: 9806
NgoMIV G'CCGG_C
Cuts at: 516 839 1117 1150 7985 516
Size: 323 278 33 6835 2337
Fragments arranged by size:
6835 2337 323 278 33
NheI G'CTAG_C
Cuts at: 3112 6660 3112
Size: 3548 6258
NotI GC'GGCC_GC
Cuts at: 525 669 959 1112 1340 1406 525
Size: 144 290 153 228 66 8925
Fragments arranged by size:
8925 290 228 153 144 66
NsiI A_TGCA'T
Cuts at: 2067 2369 2543 5890 2067
Size: 302 174 3347 5983
Fragments arranged by size:
5983 3347 302 174
NspI r_CATG'y
Cuts at: 1827 2065 2371 2664 3852 3972 3981 4191 4227
Size: 238 306 293 1188 120 9 210 36
Cuts at: 4227 4236 4755 6600 6859 7359 8266 1827
Size: 9 519 1845 259 500 907 3367
Fragments arranged by size:
3367 1845 1188 907 519 500 306 293
259 238 210 120 36 9 9
PasI CC'CwG_GG
Cuts at: 555 1473 2503 2580 5734 555
Size: 918 1030 77 3154 4627
Fragments arranged by size:
4627 3154 1030 918 77
PciI A'CATG_T
Cuts at: 1823 3977 4223 8262 1823
Size: 2154 246 4039 3367
Fragments arranged by size:
4039 3367 2154 246
PflMI CCAn_nnn'nTGG
Cuts at: 2599 2623 5223 2599
Size: 24 2600 7182
Fragments arranged by size:
7182 2600 24
PfoI T'CCnGG_A
Cuts at: 614 2622 6326 614
Size: 2008 3704 4094
Fragments arranged by size:
4094 3704 2008
PmeI GTTT'AAAC
Cuts at: 6805 6805
Size: 9806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1836 1868 2836 2868 8945 8977 1836
Size: 32 968 32 6077 32 2665
Fragments arranged by size:
6077 2665 968 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3891 6265 3891
Size: 2374 7432
PspOMI G'GGCC_C
Cuts at: 456 758 1074 2094 2506 4290 4353 4491 5737
Size: 302 316 1020 412 1784 63 138 1246
Cuts at: 5737 456
Size: 4525
Fragments arranged by size:
4525 1784 1246 1020 412 316 302 138
63
PspXI vC'TCGA_Gb
Cuts at: 6103 6103
Size: 9806
PstI C_TGCA'G
Cuts at: 785 4412 5370 6076 6237 6853 7538 785
Size: 3627 958 706 161 616 685 3053
Fragments arranged by size:
3627 3053 958 706 685 616 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9806
PvuII CAG'CTG
Cuts at: 1197 2599 6739 7231 7591 1197
Size: 1402 4140 492 360 3412
Fragments arranged by size:
4140 3412 1402 492 360
RsrII CG'GwC_CG
Cuts at: 1453 4296 8001 1453
Size: 2843 3705 3258
Fragments arranged by size:
3705 3258 2843
SacI G_AGCT'C
Cuts at: 1042 1987 3472 6820 1042
Size: 945 1485 3348 4028
Fragments arranged by size:
4028 3348 1485 945
SacII CC_GC'GG
Cuts at: 965 1022 1346 965
Size: 57 324 9425
Fragments arranged by size:
9425 324 57
SalI G'TCGA_C
Cuts at: 3271 6843 3271
Size: 3572 6234
SanDI GG'GwC_CC
Cuts at: 6265 6265
Size: 9806
SapI GCTCTTCn'nnn_
Cuts at: 5645 7829 8039 5645
Size: 2184 210 7412
Fragments arranged by size:
7412 2184 210
SbfI CC_TGCA'GG
Cuts at: 6853 6853
Size: 9806
ScaI AGT'ACT
Cuts at: 5110 9323 5110
Size: 4213 5593
SfcI C'TryA_G
Cuts at: 33 250 781 834 2123 2493 2829 2883 3036
Size: 217 531 53 1289 370 336 54 153
Cuts at: 3036 4332 4408 5366 6072 6233 6849 7534 8527
Size: 1296 76 958 706 161 616 685 993
Cuts at: 8527 8718 33
Size: 191 1121
Fragments arranged by size:
1296 1289 1121 993 958 706 685 616
531 370 336 217 191 161 153 76
54 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 668 1405 1592 4499 668
Size: 737 187 2907 5975
Fragments arranged by size:
5975 2907 737 187
SfoI GGC'GCC
Cuts at: 417 552 1374 2876 2894 4514 4712 5587 7238
Size: 135 822 1502 18 1620 198 875 1651
Cuts at: 7238 7486 417
Size: 248 2737
Fragments arranged by size:
2737 1651 1620 1502 875 822 248 198
135 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9806
SmaI CCC'GGG
Cuts at: 455 542 588 717 1080 1635 2090 2202 4358
Size: 87 46 129 363 555 455 112 2156
Cuts at: 4358 6828 455
Size: 2470 3433
Fragments arranged by size:
3433 2470 2156 555 455 363 129 112
87 46
SmlI C'TyrA_G
Cuts at: 2235 2400 3315 3453 5601 6103 6111 6961 7876
Size: 165 915 138 2148 502 8 850 915
Cuts at: 7876 8368 8630 2235
Size: 492 262 3411
Fragments arranged by size:
3411 2148 915 915 850 502 492 262
165 138 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9806
SphI G_CATG'C
Cuts at: 2371 3852 3972 6859 7359 2371
Size: 1481 120 2887 500 4818
Fragments arranged by size:
4818 2887 1481 500 120
SspI AAT'ATT
Cuts at: 5031 5904 5031
Size: 873 8933
StuI AGG'CCT
Cuts at: 5963 5963
Size: 9806
StyI C'CwwG_G
Cuts at: 107 419 1460 1640 2019 2900 3659 4098 5222
Size: 312 1041 180 379 881 759 439 1124
Cuts at: 5222 6446 6471 6650 6665 6940 7294 107
Size: 1224 25 179 15 275 354 2619
Fragments arranged by size:
2619 1224 1124 1041 881 759 439 379
354 312 275 180 179 25 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1470 3290 4076 5471 5761 6264 7389 7522 7981
Size: 1820 786 1395 290 503 1125 133 459
Cuts at: 7981 8190 1470
Size: 209 3086
Fragments arranged by size:
3086 1820 1395 1125 786 503 459 290
209 133
TatI w'GTAC_w
Cuts at: 1250 3748 3980 4226 4238 4262 5108 6777 9321
Size: 2498 232 246 12 24 846 1669 2544
Cuts at: 9321 1250
Size: 1735
Fragments arranged by size:
2544 2498 1735 1669 846 246 232 24
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 724 756 4364 4396 4863 4895 9323 9355 724
Size: 32 3608 32 467 32 4428 32 1175
Fragments arranged by size:
4428 3608 1175 467 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7603 7603
Size: 9806
XbaI T'CTAG_A
Cuts at: 6837 6837
Size: 9806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2368 6672 7301 2143
Size: 225 4304 629 4648
Fragments arranged by size:
4648 4304 629 225
XhoI C'TCGA_G
Cuts at: 6103 6103
Size: 9806
XmaI C'CCGG_G
Cuts at: 453 540 586 715 1078 1633 2088 2200 4356
Size: 87 46 129 363 555 455 112 2156
Cuts at: 4356 6826 453
Size: 2470 3433
Fragments arranged by size:
3433 2470 2156 555 455 363 129 112
87 46
ZraI GAC'GTC
Cuts at: 5691 5691
Size: 9806
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AloI AvrII BcgI BsiWI BstZ17I CspCI DraIII
FseI FspAI HpaI NruI PacI PmlI PshAI PsiI
PsrI SexAI SgrAI SnaBI SrfI SwaI XmnI