Restriction map for pF1KE1600
 (Linear) (Six-base) MAPSORT of: KIEE1600.seq  Check: 2853  from: 1  to: 6693



KIEE1600.orf

 With 182 enzymes: SgfI * 

                        July 13, 2010 13:08  ..

AatII G_ACGT'C

Cuts at:      0    5585    6693 
   Size:       5585    1108
 
AccI GT'mk_AC

Cuts at:      0    3164    6521    6693 
   Size:       3164    3357     172

  Fragments arranged by size: 

                3357    3164     172
 
Acc65I G'GTAC_C

Cuts at:      0     692    1697    4446    6693 
   Size:        692    1005    2749    2247

  Fragments arranged by size: 

                2749    2247    1005     692
 
AclI AA'CG_TT

Cuts at:      0    3335    6693 
   Size:       3335    3358
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3429    3595    5561    5633    6693 
   Size:       3429     166    1966      72    1060

  Fragments arranged by size: 

                3429    1966    1060     166      72
 
AfeI AGC'GCT

Cuts at:      0     534    1036    4627    6693 
   Size:        534     502    3591    2066

  Fragments arranged by size: 

                3591    2066     534     502
 
AflII C'TTAA_G

Cuts at:      0    5493    6693 
   Size:       5493    1200
 
AflIII A'CryG_T

Cuts at:      0    1715    3869    4115    6014    6693 
   Size:       1715    2154     246    1899     679

  Fragments arranged by size: 

                2154    1899    1715     679     246
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2306    3256    5109    6693 
   Size:       2306     950    1853    1584

  Fragments arranged by size: 

                2306    1853    1584     950
 
AleI CACnn'nnGTG

Cuts at:      0    3121    6693 
   Size:       3121    3572
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2660    3757    4372    6097    6693 
   Size:       2660    1097     615    1725     596

  Fragments arranged by size: 

                2660    1725    1097     615     596
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1674    2815    3390    6693 
   Size:       1674    1141     575    3303

  Fragments arranged by size: 

                3303    1674    1141     575
 
ApaI G_GGCC'C

Cuts at:      0     352     654     970    1990    2402    4186    4249    4387 
   Size:        352     302     316    1020     412    1784      63     138

Cuts at:   4387    5633    6693 
   Size:       1246    1060

  Fragments arranged by size: 

                1784    1246    1060    1020     412     352     316     302
                 138      63
 
ApaLI G'TGCA_C

Cuts at:      0    3652    6693 
   Size:       3652    3041
 
ApoI r'AATT_y

Cuts at:      0    5033    5462    6274    6586    6693 
   Size:       5033     429     812     312     107

  Fragments arranged by size: 

                5033     812     429     312     107
 
AscI GG'CGCG_CC

Cuts at:      0     263    6693 
   Size:        263    6430
 
AseI AT'TA_AT

Cuts at:      0    4919    6693 
   Size:       4919    1774
 
AvaI C'yCGr_G

Cuts at:      0     345     432     478     607     970    1138    1525    1980 
   Size:        345      87      46     129     363     168     387     455

Cuts at:   1980    2092    2905    3501    4002    4179    4248    5995    6693 
   Size:        112     813     596     501     177      69    1747     698

  Fragments arranged by size: 

                1747     813     698     596     501     455     387     363
                 345     177     168     129     112      87      69      46
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1480    1513    6693 
   Size:       1480      33    5180

  Fragments arranged by size: 

                5180    1480      33
 
BamHI G'GATC_C

Cuts at:      0    1380    3505    6693 
   Size:       1380    2125    3188

  Fragments arranged by size: 

                3188    2125    1380
 
BanI G'GyrC_C

Cuts at:      0     307     442     692    1264    1336    1697    2370    2766 
   Size:        307     135     250     572      72     361     673     396

Cuts at:   2766    2784    3340    4025    4404    4446    4602    5477    6693 
   Size:         18     556     685     379      42     156     875    1216

  Fragments arranged by size: 

                1216     875     685     673     572     556     396     379
                 361     307     250     156     135      72      42      18
            
 
BanII G_rGCy'C

Cuts at:      0     352     654     907     934     970    1333    1879    1990 
   Size:        352     302     253      27      36     363     546     111

Cuts at:   1990    2402    3364    3431    3776    3827    4186    4249    4387 
   Size:        412     962      67     345      51     359      63     138

Cuts at:   4387    5633    5714    6693 
   Size:       1246      81     979

  Fragments arranged by size: 

                1246     979     962     546     412     363     359     352
                 345     302     253     138     111      81      67      63
                  51      36      27
 
BbeI G_GCGC'C

Cuts at:      0     311     446    1268    2770    2788    4408    4606    5481 
   Size:        311     135     822    1502      18    1620     198     875

Cuts at:   5481    6693 
   Size:       1212

  Fragments arranged by size: 

                1620    1502    1212     875     822     311     198     135
                  18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1787    3435    5180    5201    5212    5621    6693 
   Size:       1787    1648    1745      21      11     409    1072

  Fragments arranged by size: 

                1787    1745    1648    1072     409      21      11
 
BbvCI CC'TCA_GC

Cuts at:      0     269     994    1442    1553    4325    4521    6693 
   Size:        269     725     448     111    2772     196    2172

  Fragments arranged by size: 

                2772    2172     725     448     269     196     111
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1508    2071    3992    4229    5674    6226    6693 
   Size:       1508     563    1921     237    1445     552     467

  Fragments arranged by size: 

                1921    1508    1445     563     552     467     237
 
BclI T'GATC_A

Cuts at:      0     582    5079    6693 
   Size:        582    4497    1614

  Fragments arranged by size: 

                4497    1614     582
 
BfrBI ATG'CAT

Cuts at:      0    1957    2259    2433    5780    6693 
   Size:       1957     302     174    3347     913

  Fragments arranged by size: 

                3347    1957     913     302     174
 
BglI GCCn_nnn'nGGC

Cuts at:      0     560     649     737     859     974    1015    1288    1297 
   Size:        560      89      88     122     115      41     273       9

Cuts at:   1297    1484    1610    4109    4391    6693 
   Size:        187     126    2499     282    2302

  Fragments arranged by size: 

                2499    2302     560     282     273     187     126     122
                 115      89      88      41       9
 
BglII A'GATC_T

Cuts at:      0    1741    1824    6693 
   Size:       1741      83    4869

  Fragments arranged by size: 

                4869    1741      83
 
BlpI GC'TnA_GC

Cuts at:      0     361     928    1878    5771    6693 
   Size:        361     567     950    3893     922

  Fragments arranged by size: 

                3893     950     922     567     361
 
Bme1580I G_kGCm'C

Cuts at:      0     352     454     479     654     970    1990    2375    2402 
   Size:        352     102      25     175     316    1020     385      27

Cuts at:   2402    2471    3656    4028    4186    4249    4387    5633    6121 
   Size:         69    1185     372     158      63     138    1246     488

Cuts at:   6121    6693 
   Size:        572

  Fragments arranged by size: 

                1246    1185    1020     572     488     385     372     352
                 316     175     158     138     102      69      63      27
                  25
 
BmgBI CAC'GTC

Cuts at:      0    4491    6693 
   Size:       4491    2202
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1179    1263    1677    2369    2534    3113    3525    4962 
   Size:       1179      84     414     692     165     579     412    1437

Cuts at:   4962    6313    6450    6693 
   Size:       1351     137     243

  Fragments arranged by size: 

                1437    1351    1179     692     579     414     412     243
                 165     137      84
 
BmtI G_CTAG'C

Cuts at:      0    3008    6556    6693 
   Size:       3008    3548     137

  Fragments arranged by size: 

                3548    3008     137
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3213    3215    6693 
   Size:       3213       2    3478

  Fragments arranged by size: 

                3478    3213       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1466    1956    2331    3463    4076    4296    4581    4654 
   Size:       1466     490     375    1132     613     220     285      73

Cuts at:   4654    5722    6214    6693 
   Size:       1068     492     479

  Fragments arranged by size: 

                1466    1132    1068     613     492     490     479     375
                 285     220      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     269     994    1442    1553    4325    4521    6693 
   Size:        269     725     448     111    2772     196    2172

  Fragments arranged by size: 

                2772    2172     725     448     269     196     111
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2148    2277    3192    3330    6024    6693 
   Size:       2148     129     915     138    2694     669

  Fragments arranged by size: 

                2694    2148     915     669     138     129
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1392    5051    6190    6693 
   Size:       1392    3659    1139     503

  Fragments arranged by size: 

                3659    1392    1139     503
 
BsaAI yAC'GTr

Cuts at:      0    4901    6693 
   Size:       4901    1792
 
BsaHI Gr'CG_yC

Cuts at:      0     308     443    1265    2767    2785    4405    4570    4603 
   Size:        308     135     822    1502      18    1620     165      33

Cuts at:   4603    5252    5478    5582    6693 
   Size:        649     226     104    1111

  Fragments arranged by size: 

                1620    1502    1111     822     649     308     226     165
                 135     104      33      18
 
BsaWI w'CCGG_w

Cuts at:      0    3889    5684    6693 
   Size:       3889    1795    1009

  Fragments arranged by size: 

                3889    1795    1009
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     616     646    4755    4785    6693 
   Size:        616      30    4109      30    1908

  Fragments arranged by size: 

                4109    1908     616      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     785     788     791     803     806     809     812     815 
   Size:        785       3       3      12       3       3       3       3

Cuts at:    815     818     821     836    1742    3003    3081    3439    5152 
   Size:          3       3      15     906    1261      78     358    1713

Cuts at:   5152    5194    5203    5459    5723    5953    6242    6693 
   Size:         42       9     256     264     230     289     451

  Fragments arranged by size: 

                1713    1261     906     785     451     358     289     264
                 256     230      78      42      15      12       9       3
                   3       3       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     568    1102    1153    1688    2355    2491    2995    3132 
   Size:        568     534      51     535     667     136     504     137

Cuts at:   3132    3588    4606    4611    5062    6693 
   Size:        456    1018       5     451    1631

  Fragments arranged by size: 

                1631    1018     667     568     535     534     504     456
                 451     137     136      51       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3374    5380    5392    6088    6418    6552    6693 
   Size:       3374    2006      12     696     330     134     141

  Fragments arranged by size: 

                3374    2006     696     330     141     134      12
 
BsiEI CG_ry'CG

Cuts at:      0     420     470     503     564     854    1007    1235    1301 
   Size:        420      50      33      61     290     153     228      66

Cuts at:   1301    3168    3954    6693 
   Size:       1867     786    2739

  Fragments arranged by size: 

                2739    1867     786     420     290     228     153      66
                  61      50      33
 
BsiHKAI G_wGCw'C

Cuts at:      0     431     934    1879    3364    3656    5432    5919    6693 
   Size:        431     503     945    1485     292    1776     487     774

  Fragments arranged by size: 

                1776    1485     945     774     503     487     431     292
            
 
BsmI GAATG_Cn'

Cuts at:      0    1547    2486    6693 
   Size:       1547     939    4207

  Fragments arranged by size: 

                4207    1547     939
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    5717    6693 
   Size:       5717     976
 
Bsp1286I G_dGCh'C

Cuts at:      0     352     431     454     479     654     907     934     970 
   Size:        352      79      23      25     175     253      27      36

Cuts at:    970    1333    1879    1990    2375    2402    2471    3364    3431 
   Size:        363     546     111     385      27      69     893      67

Cuts at:   3431    3656    3776    3827    4028    4186    4249    4387    5432 
   Size:        225     120      51     201     158      63     138    1045

Cuts at:   5432    5633    5714    5919    6121    6693 
   Size:        201      81     205     202     572

  Fragments arranged by size: 

                1045     893     572     546     385     363     352     253
                 225     205     202     201     201     175     158     138
                 120     111      81      79      69      67      63      51
                  36      27      27      25      23
 
BspEI T'CCGG_A

Cuts at:      0    3889    6693 
   Size:       3889    2804
 
BspHI T'CATG_A

Cuts at:      0    4467    6693 
   Size:       4467    2226
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2978    5494    6693 
   Size:       2978    2516    1199

  Fragments arranged by size: 

                2978    2516    1199
 
BsrBI CCG'CTC

Cuts at:      0     548     832    6581    6693 
   Size:        548     284    5749     112

  Fragments arranged by size: 

                5749     548     284     112
 
BsrDI GCAATG_nn'

Cuts at:      0    3902    4356    5605    5832    6693 
   Size:       3902     454    1249     227     861

  Fragments arranged by size: 

                3902    1249     861     454     227
 
BsrFI r'CCGG_y

Cuts at:      0     408     465     731    1009    1042    6605    6693 
   Size:        408      57     266     278      33    5563      88

  Fragments arranged by size: 

                5563     408     278     266      88      57      33
 
BsrGI T'GTAC_A

Cuts at:      0    3872    4118    4154    6693 
   Size:       3872     246      36    2539

  Fragments arranged by size: 

                3872    2539     246      36
 
BssHII G'CGCG_C

Cuts at:      0     263    1157    6693 
   Size:        263     894    5536

  Fragments arranged by size: 

                5536     894     263
 
BssSI C'ACGA_G

Cuts at:      0    1902    4105    5893    6598    6693 
   Size:       1902    2203    1788     705      95

  Fragments arranged by size: 

                2203    1902    1788     705      95
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1563    1674    1888    6693 
   Size:       1563     111     214    4805

  Fragments arranged by size: 

                4805    1563     214     111
 
BstEII G'GTnAC_C

Cuts at:      0    3568    4719    6693 
   Size:       3568    1151    1974

  Fragments arranged by size: 

                3568    1974    1151
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2434    2458    3538    4975    6693 
   Size:       2434      24    1080    1437    1718

  Fragments arranged by size: 

                2434    1718    1437    1080      24
 
BstYI r'GATC_y

Cuts at:      0    1360    1380    1741    1824    3505    4256    6138    6693 
   Size:       1360      20     361      83    1681     751    1882     555

  Fragments arranged by size: 

                1882    1681    1360     751     555     361      83      20
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    4847    6693 
   Size:       4847    1846
 
BtgI C'CryG_G

Cuts at:      0     854     911    1235    1352    1532    1853    2792    3221 
   Size:        854      57     324     117     180     321     939     429

Cuts at:   3221    3551    4700    6338    6557    6693 
   Size:        330    1149    1638     219     136

  Fragments arranged by size: 

                1638    1149     939     854     429     330     324     321
                 219     180     136     117      57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1339    5217    5231    5730    5790    6693 
   Size:       1339    3878      14     499      60     903

  Fragments arranged by size: 

                3878    1339     903     499      60      14
 
BtsI GCAGTG_nn'

Cuts at:      0    2636    3357    4579    6693 
   Size:       2636     721    1222    2114

  Fragments arranged by size: 

                2636    2114    1222     721
 
ClaI AT'CG_AT

Cuts at:      0    5576    6693 
   Size:       5576    1117
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3531    4575    6693 
   Size:       3531    1044    2118

  Fragments arranged by size: 

                3531    2118    1044
 
EaeI y'GGCC_r

Cuts at:      0     330     417     500     561     851    1004    1044    1221 
   Size:        330      87      83      61     290     153      40     177

Cuts at:   1221    1232    1298    1535    3058    3606    5596    6029    6245 
   Size:         11      66     237    1523     548    1990     433     216

Cuts at:   6245    6539    6560    6693 
   Size:        294      21     133

  Fragments arranged by size: 

                1990    1523     548     433     330     294     290     237
                 216     177     153     133      87      83      66      61
                  40      21      11
 
EagI C'GGCC_G

Cuts at:      0     417     500     561     851    1004    1232    1298    6693 
   Size:        417      83      61     290     153     228      66    5395

  Fragments arranged by size: 

                5395     417     290     228     153      83      66      61
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1390    3182    5537    6693 
   Size:       1390    1792    2355    1156

  Fragments arranged by size: 

                2355    1792    1390    1156
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     608     618     624     782    6693 
   Size:        608      10       6     158    5911

  Fragments arranged by size: 

                5911     608     158      10       6
 
EcoICRI GAG'CTC

Cuts at:      0     932    1877    3362    6693 
   Size:        932     945    1485    3331

  Fragments arranged by size: 

                3331    1485     945     932
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1466    1956    2331    3429    3463    3595    4076    4296 
   Size:       1466     490     375    1098      34     132     481     220

Cuts at:   4296    4581    4654    5561    5633    5722    6214    6693 
   Size:        285      73     907      72      89     492     479

  Fragments arranged by size: 

                1466    1098     907     492     490     481     479     375
                 285     220     132      89      73      72      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     997    4227    4415    6693 
   Size:        997    3230     188    2278

  Fragments arranged by size: 

                3230    2278     997     188
 
EcoO109I rG'GnC_Cy

Cuts at:      0     650     651     967    1986    2398    3783    3994    4245 
   Size:        650       1     316    1019     412    1385     211     251

Cuts at:   4245    4321    4383    4384    5630    6157    6693 
   Size:         76      62       1    1246     527     536

  Fragments arranged by size: 

                1385    1246    1019     650     536     527     412     316
                 251     211      76      62       1       1
 
EcoRI G'AATT_C

Cuts at:      0    5033    6586    6693 
   Size:       5033    1553     107

  Fragments arranged by size: 

                5033    1553     107
 
EcoRV GAT'ATC

Cuts at:      0    4836    6618    6626    6693 
   Size:       4836    1782       8      67

  Fragments arranged by size: 

                4836    1782      67       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2318    3804    5673    6693 
   Size:       2318    1486    1869    1020

  Fragments arranged by size: 

                2318    1869    1486    1020
 
HaeII r_GCGC'y

Cuts at:      0     311     446     536    1038    1268    2770    2788    3924 
   Size:        311     135      90     502     230    1502      18    1136

Cuts at:   3924    4104    4408    4606    4629    5481    6346    6693 
   Size:        180     304     198      23     852     865     347

  Fragments arranged by size: 

                1502    1136     865     852     502     347     311     304
                 230     198     180     135      90      23      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1068    1100    1554    1586    2292    2324    2821    2853 
   Size:       1068      32     454      32     706      32     497      32

Cuts at:   2853    2899    2931    3242    3274    3649    3681    4449    4481 
   Size:         46      32     311      32     375      32     768      32

Cuts at:   4481    5095    5127    5572    5604    6693 
   Size:        614      32     445      32    1089

  Fragments arranged by size: 

                1089    1068     768     706     614     497     454     445
                 375     311      46      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    3165    3536    5346    6693 
   Size:       3165     371    1810    1347

  Fragments arranged by size: 

                3165    1810    1347     371
 
HindIII A'AGCT_T

Cuts at:      0    6426    6693 
   Size:       6426     267
 
KasI G'GCGC_C

Cuts at:      0     307     442    1264    2766    2784    4404    4602    5477 
   Size:        307     135     822    1502      18    1620     198     875

Cuts at:   5477    6693 
   Size:       1216

  Fragments arranged by size: 

                1620    1502    1216     875     822     307     198     135
                  18
 
KpnI G_GTAC'C

Cuts at:      0     696    1701    4450    6693 
   Size:        696    1005    2749    2243

  Fragments arranged by size: 

                2749    2243    1005     696
 
MfeI C'AATT_G

Cuts at:      0    1502    6693 
   Size:       1502    5191
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1498    3273    6172    6693 
   Size:        254    1244    1775    2899     521

  Fragments arranged by size: 

                2899    1775    1244     521     254
 
MscI TGG'CCA

Cuts at:      0    3060    6031    6247    6562    6693 
   Size:       3060    2971     216     315     131

  Fragments arranged by size: 

                3060    2971     315     216     131
 
MslI CAynn'nnrTG

Cuts at:      0      89     110     961    3026    3121    3181    3739    5785 
   Size:         89      21     851    2065      95      60     558    2046

Cuts at:   5785    6394    6487    6693 
   Size:        609      93     206

  Fragments arranged by size: 

                2065    2046     851     609     558     206      95      93
                  89      60      21
 
MspA1I CmG'CkG

Cuts at:      0     357     785     856     913    1089    1237    1279    1494 
   Size:        357     428      71      57     176     148      42     215

Cuts at:   1494    2390    2491    4299    5257    6631    6693 
   Size:        896     101    1808     958    1374      62

  Fragments arranged by size: 

                1808    1374     958     896     428     357     215     176
                 148     101      71      62      57      42
 
NaeI GCC'GGC

Cuts at:      0     410     733    1011    1044    6693 
   Size:        410     323     278      33    5649

  Fragments arranged by size: 

                5649     410     323     278      33
 
NarI GG'CG_CC

Cuts at:      0     308     443    1265    2767    2785    4405    4603    5478 
   Size:        308     135     822    1502      18    1620     198     875

Cuts at:   5478    6693 
   Size:       1215

  Fragments arranged by size: 

                1620    1502    1215     875     822     308     198     135
                  18
 
NcoI C'CATG_G

Cuts at:      0    1352    1532    2792    3551    6338    6557    6693 
   Size:       1352     180    1260     759    2787     219     136

  Fragments arranged by size: 

                2787    1352    1260     759     219     180     136
 
NdeI CA'TA_TG

Cuts at:      0    4070    6693 
   Size:       4070    2623
 
NgoMIV G'CCGG_C

Cuts at:      0     408     731    1009    1042    6693 
   Size:        408     323     278      33    5651

  Fragments arranged by size: 

                5651     408     323     278      33
 
NheI G'CTAG_C

Cuts at:      0    3004    6552    6693 
   Size:       3004    3548     141

  Fragments arranged by size: 

                3548    3004     141
 
NotI GC'GGCC_GC

Cuts at:      0     417     561     851    1004    1232    1298    6693 
   Size:        417     144     290     153     228      66    5395

  Fragments arranged by size: 

                5395     417     290     228     153     144      66
 
NsiI A_TGCA'T

Cuts at:      0    1959    2261    2435    5782    6693 
   Size:       1959     302     174    3347     911

  Fragments arranged by size: 

                3347    1959     911     302     174
 
NspI r_CATG'y

Cuts at:      0    1719    1957    2263    2556    3744    3864    3873    4083 
   Size:       1719     238     306     293    1188     120       9     210

Cuts at:   4083    4119    4128    4647    6492    6693 
   Size:         36       9     519    1845     201

  Fragments arranged by size: 

                1845    1719    1188     519     306     293     238     210
                 201     120      36       9       9
 
PasI CC'CwG_GG

Cuts at:      0     447    1365    2395    2472    5626    6693 
   Size:        447     918    1030      77    3154    1067

  Fragments arranged by size: 

                3154    1067    1030     918     447      77
 
PciI A'CATG_T

Cuts at:      0    1715    3869    4115    6693 
   Size:       1715    2154     246    2578

  Fragments arranged by size: 

                2578    2154    1715     246
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2491    2515    5115    6693 
   Size:       2491      24    2600    1578

  Fragments arranged by size: 

                2600    2491    1578      24
 
PfoI T'CCnGG_A

Cuts at:      0     506    2514    6218    6693 
   Size:        506    2008    3704     475

  Fragments arranged by size: 

                3704    2008     506     475
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1728    1760    2728    2760    6693 
   Size:       1728      32     968      32    3933

  Fragments arranged by size: 

                3933    1728     968      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3783    6157    6693 
   Size:       3783    2374     536

  Fragments arranged by size: 

                3783    2374     536
 
PspOMI G'GGCC_C

Cuts at:      0     348     650     966    1986    2398    4182    4245    4383 
   Size:        348     302     316    1020     412    1784      63     138

Cuts at:   4383    5629    6693 
   Size:       1246    1064

  Fragments arranged by size: 

                1784    1246    1064    1020     412     348     316     302
                 138      63
 
PspXI vC'TCGA_Gb

Cuts at:      0    5995    6693 
   Size:       5995     698
 
PstI C_TGCA'G

Cuts at:      0     677    4304    5262    5968    6129    6693 
   Size:        677    3627     958     706     161     564

  Fragments arranged by size: 

                3627     958     706     677     564     161
 
PvuII CAG'CTG

Cuts at:      0    1089    2491    6631    6693 
   Size:       1089    1402    4140      62

  Fragments arranged by size: 

                4140    1402    1089      62
 
RsrII CG'GwC_CG

Cuts at:      0    1345    4188    6693 
   Size:       1345    2843    2505

  Fragments arranged by size: 

                2843    2505    1345
 
SacI G_AGCT'C

Cuts at:      0     934    1879    3364    6693 
   Size:        934     945    1485    3329

  Fragments arranged by size: 

                3329    1485     945     934
 
SacII CC_GC'GG

Cuts at:      0     857     914    1238    6693 
   Size:        857      57     324    5455

  Fragments arranged by size: 

                5455     857     324      57
 
SalI G'TCGA_C

Cuts at:      0    3163    6693 
   Size:       3163    3530
 
SanDI GG'GwC_CC

Cuts at:      0    6157    6693 
   Size:       6157     536
 
SapI GCTCTTCn'nnn_

Cuts at:      0    5537    6693 
   Size:       5537    1156
 
ScaI AGT'ACT

Cuts at:      0    5002    6693 
   Size:       5002    1691
 
SfcI C'TryA_G

Cuts at:      0     142     673     726    2015    2385    2721    2775    2928 
   Size:        142     531      53    1289     370     336      54     153

Cuts at:   2928    4224    4300    5258    5964    6125    6693 
   Size:       1296      76     958     706     161     568

  Fragments arranged by size: 

                1296    1289     958     706     568     531     370     336
                 161     153     142      76      54      53
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     560    1297    1484    4391    6693 
   Size:        560     737     187    2907    2302

  Fragments arranged by size: 

                2907    2302     737     560     187
 
SfoI GGC'GCC

Cuts at:      0     309     444    1266    2768    2786    4406    4604    5479 
   Size:        309     135     822    1502      18    1620     198     875

Cuts at:   5479    6693 
   Size:       1214

  Fragments arranged by size: 

                1620    1502    1214     875     822     309     198     135
                  18
 
SmaI CCC'GGG

Cuts at:      0     347     434     480     609     972    1527    1982    2094 
   Size:        347      87      46     129     363     555     455     112

Cuts at:   2094    4250    6693 
   Size:       2156    2443

  Fragments arranged by size: 

                2443    2156     555     455     363     347     129     112
                  87      46
 
SmlI C'TyrA_G

Cuts at:      0    2127    2292    3207    3345    5493    5995    6003    6693 
   Size:       2127     165     915     138    2148     502       8     690

  Fragments arranged by size: 

                2148    2127     915     690     502     165     138       8
            
 
SphI G_CATG'C

Cuts at:      0    2263    3744    3864    6693 
   Size:       2263    1481     120    2829

  Fragments arranged by size: 

                2829    2263    1481     120
 
SspI AAT'ATT

Cuts at:      0    4923    5796    6693 
   Size:       4923     873     897

  Fragments arranged by size: 

                4923     897     873
 
StuI AGG'CCT

Cuts at:      0    5855    6693 
   Size:       5855     838
 
StyI C'CwwG_G

Cuts at:      0     311    1352    1532    1911    2792    3551    3990    5114 
   Size:        311    1041     180     379     881     759     439    1124

Cuts at:   5114    6338    6363    6542    6557    6693 
   Size:       1224      25     179      15     136

  Fragments arranged by size: 

                1224    1124    1041     881     759     439     379     311
                 180     179     136      25      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1362    3182    3968    5363    5653    6156    6693 
   Size:       1362    1820     786    1395     290     503     537

  Fragments arranged by size: 

                1820    1395    1362     786     537     503     290
 
TatI w'GTAC_w

Cuts at:      0    1142    3640    3872    4118    4130    4154    5000    6669 
   Size:       1142    2498     232     246      12      24     846    1669

Cuts at:   6669    6693 
   Size:         24

  Fragments arranged by size: 

                2498    1669    1142     846     246     232      24      24
                  12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     616     648    4256    4288    4755    4787    6693 
   Size:        616      32    3608      32     467      32    1906

  Fragments arranged by size: 

                3608    1906     616     467      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2035    2260    6564    6693 
   Size:       2035     225    4304     129

  Fragments arranged by size: 

                4304    2035     225     129
 
XhoI C'TCGA_G

Cuts at:      0    5995    6693 
   Size:       5995     698
 
XmaI C'CCGG_G

Cuts at:      0     345     432     478     607     970    1525    1980    2092 
   Size:        345      87      46     129     363     555     455     112

Cuts at:   2092    4248    6693 
   Size:       2156    2445

  Fragments arranged by size: 

                2445    2156     555     455     363     345     129     112
                  87      46
 
ZraI GAC'GTC

Cuts at:      0    5583    6693 
   Size:       5583    1110
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AgeI     AloI    AsiSI    AvrII     BcgI    BsaBI    BsiWI
    BstBI  BstZ17I    CspCI     DraI   DraIII     FseI     FspI    FspAI
     HpaI     MluI     NruI     PacI     PmeI     PmlI    PshAI     PsiI
     PsrI     PvuI     SbfI    SexAI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SwaI  Tth111I     XbaI     XmnI
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