Restriction map for pF1KB5915
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

Acc65I      AccIII      AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AvrII       BamHI       BbrPI       BbsI        BclI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BseAI       BsiHKAI     BsiWI       
Bsp1407I    BspDI       BspEI       BsrDI       BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        DsaI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MslI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       PvuI        RsrII       SacI        SacII       
SapI        SfiI        SfuI        SmaI        SnaBI       
SpeI        SspBI       SspI        StyI        SwaI        
TfiI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1380  GTCGAC               GT^[AC][GT]AC
AclI          937  AACGTT               AA^CGTT
Asp700I      1438  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          715  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         810  TGCGCA               TGC^GCA
BalI          695  TGGCCA               TGG^CCA
BanI          981  GGCGCC               G^G[CT][GA]CC
BbeI          981  GGCGCC               GGCGC^C
BcgI          957  CGAGCAGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1051  GGATAC               GTATCC(6/5)
BfaI          791  CTAG                 C^TAG
BglI          211  GCCGGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         800  AGATCT               A^GATCT
BsaWI        1067  ACCGGA               [AT]^CCGG[AT]
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BsmBI        1367  GAGACG               CGTCTC(1/5)
BsmI          813  GCATTC               GAATGC(1/-1)
BspHI        1358  TCATGA               T^CATGA
BsrBI         102  CCGCTC               CCGCTC(-3/-3)
BssHII        173  GCGCGC               G^CGCGC
BstXI         992  CCAATCCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco52I          5  CGGCCG               C^GGCCG
FspI          810  TGCGCA               TGC^GCA
HaeII         981  GGCGCC               [GA]GCGC^[CT]
HgaI         1113  GACGC                GACGC(5/10)
HincII       1380  GTCGAC               GT[CT]^[GA]AC
HindII       1380  GTCGAC               GT[CT]^[GA]AC
HinfI         576  GACTC                G^A[ACGT]TC
KasI          981  GGCGCC               G^GCGCC
MaeI          791  CTAG                 C^TAG
MluNI         695  TGGCCA               TGG^CCA
MscI          695  TGGCCA               TGG^CCA
NarI          981  GGCGCC               GG^CGCC
PflFI         715  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          576  GACTC                GAGTC(4/5)
PpuMI         612  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         576  GACTCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      937  AACGTT               AA^CGTT
RcaI         1358  TCATGA               T^CATGA
SalI         1380  GTCGAC               G^TCGAC
ScaI         1088  AGTACT               AGT^ACT
SexAI         716  ACCTGGT              A^CC[AT]GGT
SfoI          981  GGCGCC               GGC^GCC
SgrAI         210  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          110  GCATGC               GCATG^C
StuI          440  AGGCCT               AGG^CCT
TspRI        1279  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       715  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1175  CCATCTACATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1438  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AatII        1132  GACGTC               GACGT^C
             1369  GACGTC              
AcsI          972  GAATTT               [GA]^AATT[CT]
             1034  AAATTT              
AlwI           24  GATCC                GGATC(4/5)
              785  GGATC               
ApoI          972  GAATTT               [GA]^AATT[CT]
             1034  AAATTT              
AvaI          595  CTCGGG               C^[CT]CG[GA]G
              955  CCCGAG              
AvaII          36  GGACC                G^G[AT]CC
              613  GGACC               
BbvCI         168  CCTCAGC              CCTCAGC(-5/-2)
              552  GCTGAGG             
BsoBI         595  CTCGGG               C^[CT]CG[GA]G
              955  CCCGAG              
BspLU11I     1106  ACATGT               A^CATGT
             1126  ACATGT              
BsrFI         136  GCCGGC               [GA]^CCGG[CT]
              211  GCCGGC              
BstAPI        153  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              354  GCAGCTGCTGC         
BstYI          23  AGATCC               [GA]^GATC[CT]
              800  AGATCT              
Cfr10I        136  GCCGGC               [GA]^CCGG[CT]
              211  GCCGGC              
DraIII        472  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1396  CACCGAGTG           
EaeI            5  CGGCCG               [CT]^GGCC[GA]
              695  TGGCCA              
MflI           23  AGATCC               [GA]^GATC[CT]
              800  AGATCT              
MseI          896  TTAA                 T^TAA
             1319  TTAA                
NaeI          136  GCCGGC               GCC^GGC
              211  GCCGGC              
NgoMIV        136  GCCGGC               G^CCGGC
              211  GCCGGC              
PvuII         157  CAGCTG               CAG^CTG
              355  CAGCTG              
SbfI          333  CCTGCAGG             CCTGCA^GG
              432  CCTGCAGG            
SfaNI         964  GATGC                GCATC(5/9)
             1349  GCATC               
SmlI         1291  CTCAAG               C^T[CT][GA]AG
             1408  CTCAAG              
Sse8387I      333  CCTGCAGG             CCTGCA^GG
              432  CCTGCAGG            
TaqI           71  TCGA                 T^CGA
             1381  TCGA                
Tru9I         896  TTAA                 T^TAA
             1319  TTAA                
TthHB8I        71  TCGA                 T^CGA
             1381  TCGA                
XhoII          23  AGATCC               [GA]^GATC[CT]
              800  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwNI       ApaI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EarI        EcoNI       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TseI        
Tsp45I      Tsp509I     Van91I      
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