Restriction map for pF1KB5915
 (Linear) (Six-base) MAPSORT of: KIBB5915.seq  Check: 802  from: 1  to: 1515



KIBB5915.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     402    1515 
   Size:        402    1113
 
AatII G_ACGT'C

Cuts at:      0    1136    1373    1515 
   Size:       1136     237     142

  Fragments arranged by size: 

                1136     237     142
 
AccI GT'mk_AC

Cuts at:      0    1381    1515 
   Size:       1381     134
 
AclI AA'CG_TT

Cuts at:      0     938    1515 
   Size:        938     577
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562    1515 
   Size:        562     953
 
AflIII A'CryG_T

Cuts at:      0    1106    1126    1301    1515 
   Size:       1106      20     175     214

  Fragments arranged by size: 

                1106     214     175      20
 
AlwNI CAG_nnn'CTG

Cuts at:      0     159     360     552    1515 
   Size:        159     201     192     963

  Fragments arranged by size: 

                 963     201     192     159
 
ApaI G_GGCC'C

Cuts at:      0     222     244    1214    1515 
   Size:        222      22     970     301

  Fragments arranged by size: 

                 970     301     222      22
 
ApoI r'AATT_y

Cuts at:      0     972    1034    1515 
   Size:        972      62     481

  Fragments arranged by size: 

                 972     481      62
 
AvaI C'yCGr_G

Cuts at:      0     595     955    1515 
   Size:        595     360     560

  Fragments arranged by size: 

                 595     560     360
 
BanI G'GyrC_C

Cuts at:      0     981    1515 
   Size:        981     534
 
BanII G_rGCy'C

Cuts at:      0     222     244     647    1214    1515 
   Size:        222      22     403     567     301

  Fragments arranged by size: 

                 567     403     301     222      22
 
BbeI G_GCGC'C

Cuts at:      0     985    1515 
   Size:        985     530
 
BbvCI CC'TCA_GC

Cuts at:      0     169     553    1515 
   Size:        169     384     962

  Fragments arranged by size: 

                 962     384     169
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     946     980    1515 
   Size:        946      34     535

  Fragments arranged by size: 

                 946     535      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1045    1515 
   Size:       1045     470
 
BglI GCCn_nnn'nGGC

Cuts at:      0     217    1515 
   Size:        217    1298
 
BglII A'GATC_T

Cuts at:      0     800    1515 
   Size:        800     715
 
Bme1580I G_kGCm'C

Cuts at:      0     222     244    1214    1515 
   Size:        222      22     970     301

  Fragments arranged by size: 

                 970     301     222      22
 
BmgBI CAC'GTC

Cuts at:      0     344    1304    1515 
   Size:        344     960     211

  Fragments arranged by size: 

                 960     344     211
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     486     581    1515 
   Size:        486      95     934

  Fragments arranged by size: 

                 934     486      95
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342    1423    1515 
   Size:        342    1081      92

  Fragments arranged by size: 

                1081     342      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     412     509     829    1515 
   Size:         52     360      97     320     686

  Fragments arranged by size: 

                 686     360     320      97      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     124     169     553     658    1515 
   Size:        124      45     384     105     857

  Fragments arranged by size: 

                 857     384     124     105      45
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1276    1393    1515 
   Size:       1276     117     122

  Fragments arranged by size: 

                1276     122     117
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     515    1058    1165    1515 
   Size:        515     543     107     350

  Fragments arranged by size: 

                 543     515     350     107
 
BsaHI Gr'CG_yC

Cuts at:      0     982    1133    1370    1515 
   Size:        982     151     237     145

  Fragments arranged by size: 

                 982     237     151     145
 
BsaWI w'CCGG_w

Cuts at:      0    1067    1515 
   Size:       1067     448
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     561     591     634     664    1288    1318    1515 
   Size:        561      30      43      30     624      30     197

  Fragments arranged by size: 

                 624     561     197      43      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     295     503     682    1515 
   Size:         25     270     208     179     833

  Fragments arranged by size: 

                 833     270     208     179      25
 
BseYI C'CCAG_C

Cuts at:      0     826    1206    1515 
   Size:        826     380     309

  Fragments arranged by size: 

                 826     380     309
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     172    1273    1515 
   Size:         40     132    1101     242

  Fragments arranged by size: 

                1101     242     132      40
 
BsiEI CG_ry'CG

Cuts at:      0       8    1515 
   Size:          8    1507
 
BsmI GAATG_Cn'

Cuts at:      0     813    1515 
   Size:        813     702
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1361    1515 
   Size:       1361     154
 
Bsp1286I G_dGCh'C

Cuts at:      0     222     244     647    1214    1515 
   Size:        222      22     403     567     301

  Fragments arranged by size: 

                 567     403     301     222      22
 
BspHI T'CATG_A

Cuts at:      0    1358    1515 
   Size:       1358     157
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     327     402     440    1515 
   Size:        327      75      38    1075

  Fragments arranged by size: 

                1075     327      75      38
 
BsrBI CCG'CTC

Cuts at:      0     104    1515 
   Size:        104    1411
 
BsrFI r'CCGG_y

Cuts at:      0     136     211    1515 
   Size:        136      75    1304

  Fragments arranged by size: 

                1304     136      75
 
BssHII G'CGCG_C

Cuts at:      0     173    1515 
   Size:        173    1342
 
BssSI C'ACGA_G

Cuts at:      0     472     586    1201    1515 
   Size:        472     114     615     314

  Fragments arranged by size: 

                 615     472     314     114
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     159     360    1515 
   Size:        159     201    1155

  Fragments arranged by size: 

                1155     201     159
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     999    1515 
   Size:        999     516
 
BstYI r'GATC_y

Cuts at:      0      23     800    1515 
   Size:         23     777     715

  Fragments arranged by size: 

                 777     715      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     477    1401    1515 
   Size:        477     924     114

  Fragments arranged by size: 

                 924     477     114
 
EaeI y'GGCC_r

Cuts at:      0       5     695    1515 
   Size:          5     690     820

  Fragments arranged by size: 

                 820     690       5
 
EagI C'GGCC_G

Cuts at:      0       5    1515 
   Size:          5    1510
 
EarI CTCTTCn'nnn_

Cuts at:      0     769     862    1104    1163    1515 
   Size:        769      93     242      59     352

  Fragments arranged by size: 

                 769     352     242      93      59
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      16    1515 
   Size:         16    1499
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     412     509     562     829    1515 
   Size:         52     360      97      53     267     686

  Fragments arranged by size: 

                 686     360     267      97      53      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     127     409     436    1515 
   Size:        127     282      27    1079

  Fragments arranged by size: 

                1079     282     127      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     218     613    1210    1515 
   Size:        120      98     395     597     305

  Fragments arranged by size: 

                 597     395     305     120      98
 
FspI TGC'GCA

Cuts at:      0     812    1515 
   Size:        812     703
 
HaeII r_GCGC'y

Cuts at:      0     985    1515 
   Size:        985     530
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     561     593     634     666    1288    1320    1515 
   Size:        561      32      41      32     622      32     195

  Fragments arranged by size: 

                 622     561     195      41      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1382    1515 
   Size:       1382     133
 
KasI G'GCGC_C

Cuts at:      0     981    1515 
   Size:        981     534
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1127    1515 
   Size:       1127     388
 
MscI TGG'CCA

Cuts at:      0     697    1515 
   Size:        697     818
 
MspA1I CmG'CkG

Cuts at:      0      41     159     357     693     808    1515 
   Size:         41     118     198     336     115     707

  Fragments arranged by size: 

                 707     336     198     118     115      41
 
NaeI GCC'GGC

Cuts at:      0     138     213    1515 
   Size:        138      75    1302

  Fragments arranged by size: 

                1302     138      75
 
NarI GG'CG_CC

Cuts at:      0     982    1515 
   Size:        982     533
 
NgoMIV G'CCGG_C

Cuts at:      0     136     211    1515 
   Size:        136      75    1304

  Fragments arranged by size: 

                1304     136      75
 
NspI r_CATG'y

Cuts at:      0     114    1110    1130    1245    1515 
   Size:        114     996      20     115     270

  Fragments arranged by size: 

                 996     270     115     114      20
 
PciI A'CATG_T

Cuts at:      0    1106    1126    1515 
   Size:       1106      20     389

  Fragments arranged by size: 

                1106     389      20
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     504     839    1206    1462    1515 
   Size:        504     335     367     256      53

  Fragments arranged by size: 

                 504     367     335     256      53
 
PfoI T'CCnGG_A

Cuts at:      0     749    1515 
   Size:        749     766
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     670     702    1515 
   Size:        670      32     813

  Fragments arranged by size: 

                 813     670      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     613    1515 
   Size:        613     902
 
PshAI GACnn'nnGTC

Cuts at:      0     580    1515 
   Size:        580     935
 
PspOMI G'GGCC_C

Cuts at:      0     218     240    1210    1515 
   Size:        218      22     970     305

  Fragments arranged by size: 

                 970     305     218      22
 
PstI C_TGCA'G

Cuts at:      0     338     356     437    1515 
   Size:        338      18      81    1078

  Fragments arranged by size: 

                1078     338      81      18
 
PvuII CAG'CTG

Cuts at:      0     159     357    1515 
   Size:        159     198    1158

  Fragments arranged by size: 

                1158     198     159
 
SalI G'TCGA_C

Cuts at:      0    1380    1515 
   Size:       1380     135
 
SanDI GG'GwC_CC

Cuts at:      0     613    1515 
   Size:        613     902
 
SbfI CC_TGCA'GG

Cuts at:      0     338     437    1515 
   Size:        338      99    1078

  Fragments arranged by size: 

                1078     338      99
 
ScaI AGT'ACT

Cuts at:      0    1090    1515 
   Size:       1090     425
 
SexAI A'CCwGG_T

Cuts at:      0     716    1515 
   Size:        716     799
 
SfcI C'TryA_G

Cuts at:      0     334     352     433    1515 
   Size:        334      18      81    1082

  Fragments arranged by size: 

                1082     334      81      18
 
SfoI GGC'GCC

Cuts at:      0     983    1515 
   Size:        983     532
 
SgrAI Cr'CCGG_yG

Cuts at:      0     211    1515 
   Size:        211    1304
 
SmlI C'TyrA_G

Cuts at:      0    1291    1408    1515 
   Size:       1291     117     107

  Fragments arranged by size: 

                1291     117     107
 
SphI G_CATG'C

Cuts at:      0     114    1515 
   Size:        114    1401
 
StuI AGG'CCT

Cuts at:      0     442    1515 
   Size:        442    1073
 
TatI w'GTAC_w

Cuts at:      0    1088    1412    1515 
   Size:       1088     324     103

  Fragments arranged by size: 

                1088     324     103
 
Tth111I GACn'n_nGTC

Cuts at:      0     718    1515 
   Size:        718     797
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1182    1515 
   Size:       1182     333
 
XmnI GAAnn'nnTTC

Cuts at:      0    1442    1515 
   Size:       1442      73
 
ZraI GAC'GTC

Cuts at:      0    1134    1371    1515 
   Size:       1134     237     144

  Fragments arranged by size: 

                1134     237     144
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI   AflIII    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstXI    BstYI   DraIII     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfoI    SgrAI     SmlI     SphI     StuI     TatI  Tth111I     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII     AgeI     AhdI     AleI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
     BclI    BfrBI     BlpI     BmtI    BsaAI    BsaBI  BsiHKAI    BsiWI
    BspEI    BsrDI    BsrGI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI  EcoICRI    EcoRI
    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SapI     SfiI     SgfI     SmaI    SnaBI
     SpeI     SrfI     SspI     StyI     SwaI    TaqII     TstI     XbaI
     XhoI     XmaI
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