Restriction map for pF1KE1797
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        AlwNI       AocI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BamHI       BbrPI       BclI        
BfrI        BlnI        BsaAI       BsaBI       BsgI        
BsiWI       BsmI        Bsp1407I    BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      CpoI        DraIII      DrdI        
Eam1105I    Eco81I      FbaI        FseI        FspI        
HgaI        HindIII     HpaI        KpnI        MaeII       
MamI        MfeI        MluI        MunI        NcoI        
NdeI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Alw44I       1615  GTGCAC               G^TGCAC
Aor51HI         7  AGCGCT               AGC^GCT
ApaLI        1615  GTGCAC               G^TGCAC
Asp700I      2317  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          234  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI         1297  GAAGAC               GAAGAC(2/6)
BbvCI        2113  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2427  CGATGAGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         2110  CTAG                 C^TAG
BglII        2459  AGATCT               A^GATCT
BpuAI        1297  GAAGAC               GAAGAC(2/6)
BsaI           94  GAGACC               GGTCTC(1/5)
BsiEI        1469  CGGCCG               CG[GA][CT]^CG
BspDI        2524  ATCGAT               AT^CGAT
BspMI        2260  GCAGGT               ACCTGC(4/8)
BsrFI        1973  GCCGGC               [GA]^CCGG[CT]
BstEII        132  GGTCACC              G^GT[ACGT]ACC
BstPI         132  GGTCACC              G^GT[ACGT]ACC
Cfr10I       1973  GCCGGC               [GA]^CCGG[CT]
ClaI         2524  ATCGAT               AT^CGAT
DraI         2371  TTTAAA               TTT^AAA
EagI         1469  CGGCCG               C^GGCCG
EclXI        1469  CGGCCG               C^GGCCG
Eco47III        7  AGCGCT               AGC^GCT
Eco52I       1469  CGGCCG               C^GGCCG
EcoO65I       132  GGTCACC              G^GT[ACGT]ACC
EcoRI         962  GAATTC               G^AATTC
EcoRV         903  GATATC               GAT^ATC
EcoT22I      1009  ATGCAT               ATGCA^T
HincII        799  GTTGAC               GT[CT]^[GA]AC
HindII        799  GTTGAC               GT[CT]^[GA]AC
MaeI         2110  CTAG                 C^TAG
NaeI         1973  GCCGGC               GCC^GGC
NgoMIV       1973  GCCGGC               G^CCGGC
NheI         2109  GCTAGC               G^CTAGC
NotI         1468  GCGGCCGC             GC^GGCCGC
NsiI         1009  ATGCAT               ATGCA^T
NspI         1536  GCATGT               [GA]CATG^[CT]
PaeR7I       1125  CTCGAG               C^TCGAG
PflFI         234  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1164  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         1222  GAGCTC               GAGCT^C
SmaI         1826  CCCGGG               CCC^GGG
Tth111I       234  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1164  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2213  CCAATCCAAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1125  CTCGAG               C^TCGAG
XmaCI        1826  CCCGGG               C^CCGGG
XmaI         1826  CCCGGG               C^CCGGG
XmnI         2317  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        210  TCCGGA               T^CCGGA
             1683  TCCGGA              
AcsI          962  GAATTC               [GA]^AATT[CT]
             2321  AAATTC              
AcyI         1248  GGCGCC               G[GA]^CG[CT]C
             1483  GGCGCC              
ApaI         1793  GGGCCC               GGGCC^C
             1811  GGGCCC              
ApoI          962  GAATTC               [GA]^AATT[CT]
             2321  AAATTC              
BalI         1032  TGGCCA               TGG^CCA
             2150  TGGCCA              
BbeI         1248  GGCGCC               GGCGC^C
             1483  GGCGCC              
BglI          192  GCCGGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1628  GCCCTGGGGGC         
BsaHI        1248  GGCGCC               G[GA]^CG[CT]C
             1483  GGCGCC              
BseAI         210  TCCGGA               T^CCGGA
             1683  TCCGGA              
BsmBI        1952  CGTCTC               CGTCTC(1/5)
             2473  GAGACG              
BspEI         210  TCCGGA               T^CCGGA
             1683  TCCGGA              
BsrDI         341  GCAATG               GCAATG(2/0)
             1742  GCAATG              
BstAPI       1608  GCAGCGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1860  GCAGTGGATGC         
BstXI        2072  CCAACACGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2282  CCAGTGAGTTGG        
Hin1I        1248  GGCGCC               G[GA]^CG[CT]C
             1483  GGCGCC              
KasI         1248  GGCGCC               G^GCGCC
             1483  GGCGCC              
KspI           64  CCGCGG               CCGC^GG
             1466  CCGCGG              
MluNI        1032  TGGCCA               TGG^CCA
             2150  TGGCCA              
MroI          210  TCCGGA               T^CCGGA
             1683  TCCGGA              
MscI         1032  TGGCCA               TGG^CCA
             2150  TGGCCA              
MseI          839  TTAA                 T^TAA
             2372  TTAA                
NarI         1248  GGCGCC               GG^CGCC
             1483  GGCGCC              
PleI          641  GACTC                GAGTC(4/5)
             1939  GAGTC               
PstI         1183  CTGCAG               CTGCA^G
             1816  CTGCAG              
SacII          64  CCGCGG               CCGC^GG
             1466  CCGCGG              
SfoI         1248  GGCGCC               GGC^GCC
             1483  GGCGCC              
SmlI         1125  CTCGAG               C^T[CT][GA]AG
             1339  CTCAAG              
StuI         1925  AGGCCT               AGG^CCT
             2096  AGGCCT              
Tru9I         839  TTAA                 T^TAA
             2372  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BciVI       BcnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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