Restriction map for pF1KB5946
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaII       AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BglI        BlnI        BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BspDI       BspEI       BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeII       MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StyI        
SwaI        TfiI        Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        146  GGTACC               G^GTACC
AflIII        223  ACATGT               A^C[GA][CT]GT
AlwNI          14  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          389  GGGCCC               GGGCC^C
Asp718I       146  GGTACC               G^GTACC
AvaI          703  CCCGAG               C^[CT]CG[GA]G
BalI          645  TGGCCA               TGG^CCA
BamHI        1063  GGATCC               G^GATCC
BanI          146  GGTACC               G^G[CT][GA]CC
BbsI            3  GTCTTC               GAAGAC(2/6)
BclI         1191  TGATCA               T^GATCA
BglII         102  AGATCT               A^GATCT
BlpI          876  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      876  GCTGAGC              GC^T[ACGT]AGC
BpuAI           3  GTCTTC               GAAGAC(2/6)
BsaBI          97  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         160  CTCCTC               GAGGAG(10/8)
BsiEI         336  CGACCG               CG[GA][CT]^CG
BsmI          341  GAATGC               GAATGC(1/-1)
BsoBI         703  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      374  TGTACA               T^GTACA
BspHI        1142  TCATGA               T^CATGA
BspLU11I      223  ACATGT               A^CATGT
BsrGI         374  TGTACA               T^GTACA
BstEII        631  GGTTACC              G^GT[ACGT]ACC
BstPI         631  GGTTACC              G^GT[ACGT]ACC
CelII         876  GCTGAGC              GC^T[ACGT]AGC
DraIII       1314  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          645  TGGCCA               [CT]^GGCC[GA]
EcoO65I       631  GGTTACC              G^GT[ACGT]ACC
FauI         1329  CCCGC                CCCGC(4/6)
FbaI         1191  TGATCA               T^GATCA
KpnI          146  GGTACC               GGTAC^C
MamI           97  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         645  TGGCCA               TGG^CCA
MscI          645  TGGCCA               TGG^CCA
MspA1I       1264  CAGCTG               C[AC]G^C[GT]G
NdeI          641  CATATG               CA^TATG
PshAI         568  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1264  CAGCTG               CAG^CTG
RcaI         1142  TCATGA               T^CATGA
SapI          109  GAAGAGC              GCTCTTC(1/4)
SfcI          557  CTACAG               C^T[GA][CT]AG
SphI         1470  GCATGC               GCATG^C
SspBI         374  TGTACA               T^GTACA
StuI          525  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI         1298  CCGC                 CCGC(-3/-1)
             1330  CCGC                
AcsI         1216  AAATTC               [GA]^AATT[CT]
             1345  AAATTT              
AflII         433  CTTAAG               C^TTAAG
              967  CTTAAG              
ApoI         1216  AAATTC               [GA]^AATT[CT]
             1345  AAATTT              
BcnI          660  CCGGG                CC^[GC]GG
             1380  CCGGG               
BfrI          433  CTTAAG               C^TTAAG
              967  CTTAAG              
BsmFI         119  GTCCC                GGGAC(10/14)
              575  GTCCC               
BstXI         636  CCAAACATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1067  CCATCTTCCTGG        
BstYI         102  AGATCT               [GA]^GATC[CT]
             1063  GGATCC              
DraI           39  TTTAAA               TTT^AAA
               73  TTTAAA              
DraII         388  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              389  GGGCCCT             
EarI          919  CTCTTC               CTCTTC(1/4)
              109  GAAGAG              
EcoO109I      388  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              389  GGGCCCT             
Ksp632I       919  CTCTTC               CTCTTC(1/4)
              109  GAAGAG              
MflI          102  AGATCT               [GA]^GATC[CT]
             1063  GGATCC              
NciI          660  CCGGG                CC^[GC]GG
             1380  CCGGG               
NspI          223  ACATGT               [GA]CATG^[CT]
             1470  GCATGC              
SacI          747  GAGCTC               GAGCT^C
             1012  GAGCTC              
SfaNI         761  GCATC                GCATC(5/9)
             1111  GCATC               
XhoII         102  AGATCT               [GA]^GATC[CT]
             1063  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AfaI        AluI        AlwI        AspHI       BanII       
BbvI        BfaI        BmyI        BpmI        BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SmlI        
TaqI        Tru9I       TseI        Tsp509I     TthHB8I     
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