Restriction map for pF1KB5946
 (Linear) (Six-base) MAPSORT of: KIBB5946.seq  Check: 959  from: 1  to: 1485



KIBB5946.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0     146    1485 
   Size:        146    1339
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     942     979    1421    1485 
   Size:        415     527      37     442      64

  Fragments arranged by size: 

                 527     442     415      64      37
 
AflII C'TTAA_G

Cuts at:      0     433     967    1485 
   Size:        433     534     518

  Fragments arranged by size: 

                 534     518     433
 
AflIII A'CryG_T

Cuts at:      0     223    1485 
   Size:        223    1262
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     622    1485 
   Size:        622     863
 
AlwNI CAG_nnn'CTG

Cuts at:      0      19    1485 
   Size:         19    1466
 
ApaI G_GGCC'C

Cuts at:      0     393    1485 
   Size:        393    1092
 
ApoI r'AATT_y

Cuts at:      0    1216    1345    1485 
   Size:       1216     129     140

  Fragments arranged by size: 

                1216     140     129
 
AvaI C'yCGr_G

Cuts at:      0     703    1485 
   Size:        703     782
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     210     243    1485 
   Size:        210      33    1242

  Fragments arranged by size: 

                1242     210      33
 
BamHI G'GATC_C

Cuts at:      0    1063    1485 
   Size:       1063     422
 
BanI G'GyrC_C

Cuts at:      0     146    1485 
   Size:        146    1339
 
BanII G_rGCy'C

Cuts at:      0     161     393     751    1016    1485 
   Size:        161     232     358     265     469

  Fragments arranged by size: 

                 469     358     265     232     161
 
BclI T'GATC_A

Cuts at:      0    1191    1485 
   Size:       1191     294
 
BglII A'GATC_T

Cuts at:      0     102    1485 
   Size:        102    1383
 
BlpI GC'TnA_GC

Cuts at:      0     877    1485 
   Size:        877     608
 
Bme1580I G_kGCm'C

Cuts at:      0     393    1442    1485 
   Size:        393    1049      43

  Fragments arranged by size: 

                1049     393      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      29    1091    1485 
   Size:         29    1062     394

  Fragments arranged by size: 

                1062     394      29
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1147    1485 
   Size:       1147     338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    1026    1168    1485 
   Size:        737     289     142     317

  Fragments arranged by size: 

                 737     317     289     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794    1485 
   Size:        794     691
 
BsaBI GATnn'nnATC

Cuts at:      0     101    1485 
   Size:        101    1384
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     122     152     502     532     735     765    1485 
   Size:        122      30     350      30     203      30     720

  Fragments arranged by size: 

                 720     350     203     122      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151    1485 
   Size:        151    1334
 
BsiEI CG_ry'CG

Cuts at:      0     339    1485 
   Size:        339    1146
 
BsiHKAI G_wGCw'C

Cuts at:      0     751     883    1016    1229    1485 
   Size:        751     132     133     213     256

  Fragments arranged by size: 

                 751     256     213     133     132
 
BsmI GAATG_Cn'

Cuts at:      0     347    1485 
   Size:        347    1138
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     393     751     883    1016    1229    1442    1485 
   Size:        161     232     358     132     133     213     213      43

  Fragments arranged by size: 

                 358     232     213     213     161     133     132      43
            
 
BspHI T'CATG_A

Cuts at:      0    1142    1485 
   Size:       1142     343
 
BsrGI T'GTAC_A

Cuts at:      0     374    1485 
   Size:        374    1111
 
BstEII G'GTnAC_C

Cuts at:      0     631    1485 
   Size:        631     854
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     643    1074    1485 
   Size:        643     431     411

  Fragments arranged by size: 

                 643     431     411
 
BstYI r'GATC_y

Cuts at:      0     102    1063    1485 
   Size:        102     961     422

  Fragments arranged by size: 

                 961     422     102
 
DraI TTT'AAA

Cuts at:      0      41      75    1485 
   Size:         41      34    1410

  Fragments arranged by size: 

                1410      41      34
 
DraIII CAC_nnn'GTG

Cuts at:      0    1319    1485 
   Size:       1319     166
 
EaeI y'GGCC_r

Cuts at:      0     645    1485 
   Size:        645     840
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     925    1485 
   Size:        104     821     560

  Fragments arranged by size: 

                 821     560     104
 
EcoICRI GAG'CTC

Cuts at:      0     749    1014    1485 
   Size:        749     265     471

  Fragments arranged by size: 

                 749     471     265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     737     942     979    1026    1168    1421    1485 
   Size:        415     322     205      37      47     142     253      64

  Fragments arranged by size: 

                 415     322     253     205     142      64      47      37
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     389     390    1485 
   Size:        389       1    1095

  Fragments arranged by size: 

                1095     389       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     124     156     735     767    1485 
   Size:        124      32     579      32     718

  Fragments arranged by size: 

                 718     579     124      32      32
 
KpnI G_GTAC'C

Cuts at:      0     150    1485 
   Size:        150    1335
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     288    1485 
   Size:        288    1197
 
MscI TGG'CCA

Cuts at:      0     647    1485 
   Size:        647     838
 
MslI CAynn'nnrTG

Cuts at:      0     598     604    1390    1427    1462    1485 
   Size:        598       6     786      37      35      23

  Fragments arranged by size: 

                 786     598      37      35      23       6
 
MspA1I CmG'CkG

Cuts at:      0    1266    1485 
   Size:       1266     219
 
NdeI CA'TA_TG

Cuts at:      0     642    1485 
   Size:        642     843
 
NspI r_CATG'y

Cuts at:      0     227    1474    1485 
   Size:        227    1247      11

  Fragments arranged by size: 

                1247     227      11
 
PciI A'CATG_T

Cuts at:      0     223    1485 
   Size:        223    1262
 
PfoI T'CCnGG_A

Cuts at:      0    1280    1485 
   Size:       1280     205
 
PshAI GACnn'nnGTC

Cuts at:      0     572    1485 
   Size:        572     913
 
PspOMI G'GGCC_C

Cuts at:      0     389    1485 
   Size:        389    1096
 
PvuII CAG'CTG

Cuts at:      0    1266    1485 
   Size:       1266     219
 
SacI G_AGCT'C

Cuts at:      0     751    1016    1485 
   Size:        751     265     469

  Fragments arranged by size: 

                 751     469     265
 
SapI GCTCTTCn'nnn_

Cuts at:      0     104    1485 
   Size:        104    1381
 
SfcI C'TryA_G

Cuts at:      0     557    1485 
   Size:        557     928
 
SmlI C'TyrA_G

Cuts at:      0     433     809     967    1485 
   Size:        433     376     158     518

  Fragments arranged by size: 

                 518     433     376     158
 
SphI G_CATG'C

Cuts at:      0    1474    1485 
   Size:       1474      11
 
StuI AGG'CCT

Cuts at:      0     527    1485 
   Size:        527     958
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     353    1485 
   Size:        353    1132
 
TatI w'GTAC_w

Cuts at:      0     374    1485 
   Size:        374    1111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     122     154     502     534    1485 
   Size:        122      32     348      32     951

  Fragments arranged by size: 

                 951     348     122      32      32
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AlfI    AlwNI     ApaI     ApoI
     AvaI     BaeI    BamHI     BanI    BanII     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI    BsaBI    BsaXI    BseRI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrGI   BstEII    BstXI
    BstYI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NdeI     NspI
     PciI     PfoI    PshAI   PspOMI    PvuII     SacI     SapI     SfcI
     SmlI     SphI     StuI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BmgBI     BmtI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com