Restriction map for pF1KB9189
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HapII       
HhaI        Hin1I       HinP1I      HpaII       KasI        
KpnI        KspI        MaeI        MfeI        MluI        
MroI        MspI        MunI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          102  GTCTAC               GT^[AC][GT]AC
AccII         835  CGCG                 CG^CG
AclI          407  AACGTT               AA^CGTT
AflII        1066  CTTAAG               C^TTAAG
ApaI         1238  GGGCCC               GGGCC^C
AvaII         624  GGTCC                G^G[AT]CC
BalI           57  TGGCCA               TGG^CCA
BamHI         437  GGATCC               G^GATCC
BanII        1238  GGGCCC               G[GA]GC[CT]^C
BfrI         1066  CTTAAG               C^TTAAG
BmyI         1238  GGGCCC               G[AGT]GC[ACT]^C
BsaBI         825  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1286I     1238  GGGCCC               G[AGT]GC[ACT]^C
BspHI         599  TCATGA               T^CATGA
BstBI          38  TTCGAA               TT^CGAA
BstEII        498  GGTCACC              G^GT[ACGT]ACC
BstF5I        129  CATCC                GGATG(2/0)
BstPI         498  GGTCACC              G^GT[ACGT]ACC
BstUI         835  CGCG                 CG^CG
DdeI         1205  CTCAG                C^T[ACGT]AG
DraII        1237  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI           85  GACCAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         384  CCTGCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1237  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       498  GGTCACC              G^GT[ACGT]ACC
EcoRI         985  GAATTC               G^AATTC
FauI          872  CCCGC                CCCGC(4/6)
FokI          129  CATCC                GGATG(9/13)
HgaI          893  GACGC                GACGC(5/10)
HpaI          285  GTTAAC               GTT^AAC
MamI          825  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          57  TGGCCA               TGG^CCA
MscI           57  TGGCCA               TGG^CCA
MslI          165  CACGTTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          835  CGCG                 CG^CG
NdeI          965  CATATG               CA^TATG
NspV           38  TTCGAA               TT^CGAA
PleI          864  GACTC                GAGTC(4/5)
Psp1406I      407  AACGTT               AA^CGTT
PstI         1000  CTGCAG               CTGCA^G
PvuII          88  CAGCTG               CAG^CTG
RcaI          599  TCATGA               T^CATGA
SapI           31  GAAGAGC              GCTCTTC(1/4)
SexAI         225  ACCAGGT              A^CC[AT]GGT
SfuI           38  TTCGAA               TT^CGAA
StuI          377  AGGCCT               AGG^CCT
TfiI          116  GATTC                G^A[AT]TC
XcmI          503  CCATGAAAAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BbsI         1144  GAAGAC               GAAGAC(2/6)
              953  GTCTTC              
BbvI          713  GCAGC                GCAGC(8/12)
             1198  GCAGC               
BpuAI        1144  GAAGAC               GAAGAC(2/6)
              953  GTCTTC              
BsaI           18  GAGACC               GGTCTC(1/5)
              314  GAGACC              
BseRI        1165  GAGGAG               GAGGAG(10/8)
             1168  GAGGAG              
BsgI          577  CTGCAC               GTGCAG(16/14)
              778  GTGCAG              
BsmFI         486  GTCCC                GGGAC(10/14)
             1218  GTCCC               
BspMI         383  ACCTGC               ACCTGC(4/8)
              780  GCAGGT              
BssSI         432  CACGAG               CACGAG(-5/-1)
             1090  CACGAG              
BstXI         164  CCACGTTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1227  CCAGCAATCTGG        
BstYI         437  GGATCC               [GA]^GATC[CT]
              492  GGATCT              
Cac8I         445  GCCTGC               GC[ACGT]^[ACGT]GC
             1276  GCAAGC              
HindIII       901  AAGCTT               A^AGCTT
             1278  AAGCTT              
HinfI         116  GATTC                G^A[ACGT]TC
              864  GACTC               
MaeII         166  ACGT                 A^CGT
              408  ACGT                
MflI          437  GGATCC               [GA]^GATC[CT]
              492  GGATCT              
MspA1I         88  CAGCTG               C[AC]G^C[GT]G
             1199  CAGCGG              
SfaNI         364  GATGC                GCATC(5/9)
              825  GATGC               
SfcI         1000  CTGCAG               C^T[GA][CT]AG
             1213  CTACAG              
TseI          713  GCAGC                G^C[AT]GC
             1198  GCAGC               
Tsp45I        203  GTGAC                ^GT[GC]AC
              499  GTCAC               
XhoII         437  GGATCC               [GA]^GATC[CT]
              492  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     BsaJI       BsiYI       BslI        
BsmAI       BsrDI       BsrI        BssKI       BstNI       
Cfr13I      DpnII       DsaI        EaeI        EarI        
EcoRII      Fnu4HI      HaeIII      HincII      HindII      
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SmlI        TaqI        Tru9I       Tsp509I     
TspRI       TthHB8I     XmnI        
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