Restriction map for pF1KB9189
 (Linear) (Six-base) MAPSORT of: KIBB9189.seq  Check: 82  from: 1  to: 1287



KIBB9189.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AccI GT'mk_AC

Cuts at:      0     103    1287 
   Size:        103    1184
 
AclI AA'CG_TT

Cuts at:      0     408    1287 
   Size:        408     879
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     301     933     940    1287 
   Size:        244      57     632       7     347

  Fragments arranged by size: 

                 632     347     244      57       7
 
AflII C'TTAA_G

Cuts at:      0    1066    1287 
   Size:       1066     221
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     166     198    1287 
   Size:        166      32    1089

  Fragments arranged by size: 

                1089     166      32
 
ApaI G_GGCC'C

Cuts at:      0    1242    1287 
   Size:       1242      45
 
ApoI r'AATT_y

Cuts at:      0     193     583     985    1287 
   Size:        193     390     402     302

  Fragments arranged by size: 

                 402     390     302     193
 
BamHI G'GATC_C

Cuts at:      0     437    1287 
   Size:        437     850
 
BanII G_rGCy'C

Cuts at:      0    1242    1287 
   Size:       1242      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     946    1151    1287 
   Size:        946     205     136

  Fragments arranged by size: 

                 946     205     136
 
Bme1580I G_kGCm'C

Cuts at:      0    1242    1287 
   Size:       1242      45
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     363    1287 
   Size:        363     924
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95    1183    1287 
   Size:         95    1088     104

  Fragments arranged by size: 

                1088     104      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     308    1287 
   Size:         12     296     979

  Fragments arranged by size: 

                 979     296      12
 
BsaBI GATnn'nnATC

Cuts at:      0     829    1287 
   Size:        829     458
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1180    1183    1287 
   Size:       1180       3     104

  Fragments arranged by size: 

                1180     104       3
 
BseYI C'CCAG_C

Cuts at:      0     207    1226    1241    1287 
   Size:        207    1019      15      46

  Fragments arranged by size: 

                1019     207      46      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     799    1287 
   Size:        562     237     488

  Fragments arranged by size: 

                 562     488     237
 
Bsp1286I G_dGCh'C

Cuts at:      0    1242    1287 
   Size:       1242      45
 
BspHI T'CATG_A

Cuts at:      0     599    1287 
   Size:        599     688
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     392     771    1287 
   Size:        392     379     516

  Fragments arranged by size: 

                 516     392     379
 
BsrDI GCAATG_nn'

Cuts at:      0     276     560     624     798    1287 
   Size:        276     284      64     174     489

  Fragments arranged by size: 

                 489     284     276     174      64
 
BssSI C'ACGA_G

Cuts at:      0     432    1090    1287 
   Size:        432     658     197

  Fragments arranged by size: 

                 658     432     197
 
BstBI TT'CG_AA

Cuts at:      0      39    1287 
   Size:         39    1248
 
BstEII G'GTnAC_C

Cuts at:      0     498    1287 
   Size:        498     789
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     171    1234    1287 
   Size:        171    1063      53

  Fragments arranged by size: 

                1063     171      53
 
BstYI r'GATC_y

Cuts at:      0     437     492    1287 
   Size:        437      55     795

  Fragments arranged by size: 

                 795     437      55
 
BtgI C'CryG_G

Cuts at:      0       5      11     302    1287 
   Size:          5       6     291     985

  Fragments arranged by size: 

                 985     291       6       5
 
BtsI GCAGTG_nn'

Cuts at:      0     710    1287 
   Size:        710     577
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      91    1287 
   Size:         91    1196
 
EaeI y'GGCC_r

Cuts at:      0       2       8      57    1287 
   Size:          2       6      49    1230

  Fragments arranged by size: 

                1230      49       6       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      26     421     716     902    1287 
   Size:         26     395     295     186     385

  Fragments arranged by size: 

                 395     385     295     186      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     301     933     940    1287 
   Size:        244      57     632       7     347

  Fragments arranged by size: 

                 632     347     244      57       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     388    1287 
   Size:        388     899
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1238    1287 
   Size:       1238      49
 
EcoRI G'AATT_C

Cuts at:      0     985    1287 
   Size:        985     302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1081    1287 
   Size:       1081     206
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     675     707    1287 
   Size:        675      32     580

  Fragments arranged by size: 

                 675     580      32
 
HincII GTy'rAC

Cuts at:      0      96     287     297    1287 
   Size:         96     191      10     990

  Fragments arranged by size: 

                 990     191      96      10
 
HindIII A'AGCT_T

Cuts at:      0     901    1278    1287 
   Size:        901     377       9

  Fragments arranged by size: 

                 901     377       9
 
HpaI GTT'AAC

Cuts at:      0     287    1287 
   Size:        287    1000
 
MscI TGG'CCA

Cuts at:      0      59    1287 
   Size:         59    1228
 
MslI CAynn'nnrTG

Cuts at:      0     169    1287 
   Size:        169    1118
 
MspA1I CmG'CkG

Cuts at:      0      90    1201    1287 
   Size:         90    1111      86

  Fragments arranged by size: 

                1111      90      86
 
NdeI CA'TA_TG

Cuts at:      0     966    1287 
   Size:        966     321
 
PasI CC'CwG_GG

Cuts at:      0     767    1287 
   Size:        767     520
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     901     933    1287 
   Size:        901      32     354

  Fragments arranged by size: 

                 901     354      32
 
PspOMI G'GGCC_C

Cuts at:      0    1238    1287 
   Size:       1238      49
 
PstI C_TGCA'G

Cuts at:      0    1004    1287 
   Size:       1004     283
 
PvuII CAG'CTG

Cuts at:      0      90    1287 
   Size:         90    1197
 
SapI GCTCTTCn'nnn_

Cuts at:      0      26    1287 
   Size:         26    1261
 
SexAI A'CCwGG_T

Cuts at:      0     225    1287 
   Size:        225    1062
 
SfcI C'TryA_G

Cuts at:      0    1000    1213    1287 
   Size:       1000     213      74

  Fragments arranged by size: 

                1000     213      74
 
SmlI C'TyrA_G

Cuts at:      0     110    1066    1162    1287 
   Size:        110     956      96     125

  Fragments arranged by size: 

                 956     125     110      96
 
StuI AGG'CCT

Cuts at:      0     379    1287 
   Size:        379     908
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     510    1287 
   Size:        510     777
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     985    1180    1287 
   Size:         35     950     195     107

  Fragments arranged by size: 

                 950     195     107      35
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII     AloI     ApaI     ApoI    BamHI
    BanII     BbsI Bme1580I     BmrI    BpuEI     BsaI    BsaBI    BseRI
    BseYI     BsgI Bsp1286I    BspHI    BspMI    BsrDI    BssSI    BstBI
   BstEII    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII
     HpaI     MscI     MslI   MspA1I     NdeI     PasI     PpiI   PspOMI
     PstI    PvuII     SapI    SexAI     SfcI     SmlI     StuI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com