Restriction map for pF1KB5045
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AflIII      AgeI        AhdI        Aor51HI     ApoI        
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BcgI        
BciVI       BclI        BglI        BlnI        BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BspLU11I    BsrDI       BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI           64  GTCTAC               GT^[AC][GT]AC
AflII        1114  CTTAAG               C^TTAAG
AlwNI        1250  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1396  CCTGAGG              CC^T[ACGT]AGG
Asp700I       681  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1076  CTCGAG               C^[CT]CG[GA]G
BalI          365  TGGCCA               TGG^CCA
BbrPI        1237  CACGTG               CAC^GTG
BfrI         1114  CTTAAG               C^TTAAG
BglII        1388  AGATCT               A^GATCT
BsaAI        1237  CACGTG               [CT]AC^GT[GA]
BsaWI        1762  TCCGGT               [AT]^CCGG[AT]
BseRI         661  GAGGAG               GAGGAG(10/8)
BsoBI        1076  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1262  TGTACA               T^GTACA
BsrBI         772  GAGCGG               CCGCTC(-3/-3)
BsrFI        1452  GCCGGC               [GA]^CCGG[CT]
BsrGI        1262  TGTACA               T^GTACA
BstXI        1502  CCAAGCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1396  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1452  GCCGGC               [GA]^CCGG[CT]
EarI          834  CTCTTC               CTCTTC(1/4)
Eco81I       1396  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      1269  ATGCAT               ATGCA^T
HgaI         1069  GACGC                GACGC(5/10)
Ksp632I       834  CTCTTC               CTCTTC(1/4)
MluNI         365  TGGCCA               TGG^CCA
MscI          365  TGGCCA               TGG^CCA
NaeI         1452  GCCGGC               GCC^GGC
NcoI          714  CCATGG               C^CATGG
NgoMIV       1452  GCCGGC               G^CCGGC
NsiI         1269  ATGCAT               ATGCA^T
PaeR7I       1076  CTCGAG               C^TCGAG
PmaCI        1237  CACGTG               CAC^GTG
PmlI         1237  CACGTG               CAC^GTG
PstI          310  CTGCAG               CTGCA^G
SexAI         872  ACCTGGT              A^CC[AT]GGT
SspBI        1262  TGTACA               T^GTACA
TfiI           87  GATTC                G^A[AT]TC
XcmI         1364  CCACAGTAACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1076  CTCGAG               C^TCGAG
XmnI          681  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        233  GGTACC               G^GTACC
              869  GGTACC              
Alw44I         25  GTGCAC               G^TGCAC
              180  GTGCAC              
AlwI          267  GATCC                GGATC(4/5)
             1199  GGATC               
ApaI          124  GGGCCC               GGGCC^C
             1844  GGGCCC              
ApaLI          25  GTGCAC               G^TGCAC
              180  GTGCAC              
Asp718I       233  GGTACC               G^GTACC
              869  GGTACC              
BbvCI         116  CCTCAGC              CCTCAGC(-5/-2)
              864  GCTGAGG             
BlpI         1173  GCTCAGC              GC^T[ACGT]AGC
             1330  GCTGAGC             
Bpu1102I     1173  GCTCAGC              GC^T[ACGT]AGC
             1330  GCTGAGC             
BsaI         1079  GAGACC               GGTCTC(1/5)
             1378  GAGACC              
BsmAI        1079  GAGAC                GTCTC(1/5)
             1378  GAGAC               
BsmFI        1165  GGGAC                GGGAC(10/14)
              205  GTCCC               
BsmI           42  GAATGC               GAATGC(1/-1)
              777  GAATGC              
BssSI         150  CTCGTG               CACGAG(-5/-1)
             1018  CTCGTG              
BstEII       1275  GGTGACC              G^GT[ACGT]ACC
             1444  GGTTACC             
BstPI        1275  GGTGACC              G^GT[ACGT]ACC
             1444  GGTTACC             
BstYI        1199  GGATCT               [GA]^GATC[CT]
             1388  AGATCT              
CelII        1173  GCTCAGC              GC^T[ACGT]AGC
             1330  GCTGAGC             
EaeI          365  TGGCCA               [CT]^GGCC[GA]
              978  TGGCCG              
EcoNI         195  CCTCTCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1017  CCTCGTGGAGG         
EcoO65I      1275  GGTGACC              G^GT[ACGT]ACC
             1444  GGTTACC             
KpnI          233  GGTACC               GGTAC^C
              869  GGTACC              
MaeII        1238  ACGT                 A^CGT
             1435  ACGT                
MflI         1199  GGATCT               [GA]^GATC[CT]
             1388  AGATCT              
NheI          930  GCTAGC               G^CTAGC
             1614  GCTAGC              
PflMI         294  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1834  CCAGCACATGG         
PleI         1125  GACTC                GAGTC(4/5)
             1608  GACTC               
PpuMI         203  AGGTCCC              [GA]G^G[AT]CC[CT]
              305  AGGTCCT             
SfcI          310  CTGCAG               C^T[GA][CT]AG
             1350  CTACAG              
StuI          700  AGGCCT               AGG^CCT
             1034  AGGCCT              
Van91I        294  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1834  CCAGCACATGG         
XhoII        1199  GGATCT               [GA]^GATC[CT]
             1388  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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