Restriction map for pF1KB5045
 (Linear) (Six-base) MAPSORT of: KIBB5045.seq  Check: 8251  from: 1  to: 1959



KIBB5045.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     979    1497    1959 
   Size:        979     518     462

  Fragments arranged by size: 

                 979     518     462
 
AccI GT'mk_AC

Cuts at:      0      65    1959 
   Size:         65    1894
 
Acc65I G'GTAC_C

Cuts at:      0     233     869    1959 
   Size:        233     636    1090

  Fragments arranged by size: 

                1090     636     233
 
AflII C'TTAA_G

Cuts at:      0    1114    1959 
   Size:       1114     845
 
AleI CACnn'nnGTG

Cuts at:      0    1258    1959 
   Size:       1258     701
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     124     156    1959 
   Size:        124      32    1803

  Fragments arranged by size: 

                1803     124      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1255    1959 
   Size:       1255     704
 
ApaI G_GGCC'C

Cuts at:      0     128    1848    1959 
   Size:        128    1720     111

  Fragments arranged by size: 

                1720     128     111
 
ApaLI G'TGCA_C

Cuts at:      0      25     180    1959 
   Size:         25     155    1779

  Fragments arranged by size: 

                1779     155      25
 
AvaI C'yCGr_G

Cuts at:      0    1076    1959 
   Size:       1076     883
 
BanI G'GyrC_C

Cuts at:      0     233     507     869    1219    1658    1959 
   Size:        233     274     362     350     439     301

  Fragments arranged by size: 

                 439     362     350     301     274     233
 
BanII G_rGCy'C

Cuts at:      0     128     455     542     727    1848    1959 
   Size:        128     327      87     185    1121     111

  Fragments arranged by size: 

                1121     327     185     128     111      87
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1576    1629    1724    1959 
   Size:       1576      53      95     235

  Fragments arranged by size: 

                1576     235      95      53
 
BbvCI CC'TCA_GC

Cuts at:      0     117     865    1959 
   Size:        117     748    1094

  Fragments arranged by size: 

                1094     748     117
 
BfrBI ATG'CAT

Cuts at:      0    1271    1959 
   Size:       1271     688
 
BglII A'GATC_T

Cuts at:      0    1388    1959 
   Size:       1388     571
 
BlpI GC'TnA_GC

Cuts at:      0    1174    1331    1959 
   Size:       1174     157     628

  Fragments arranged by size: 

                1174     628     157
 
Bme1580I G_kGCm'C

Cuts at:      0      29     128     184     512    1502    1848    1959 
   Size:         29      99      56     328     990     346     111

  Fragments arranged by size: 

                 990     346     328     111      99      56      29
 
BmgBI CAC'GTC

Cuts at:      0    1436    1959 
   Size:       1436     523
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1265    1959 
   Size:       1265     694
 
BmtI G_CTAG'C

Cuts at:      0     934    1618    1959 
   Size:        934     684     341

  Fragments arranged by size: 

                 934     684     341
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     576    1959 
   Size:        576    1383
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     403     571     679     688    1959 
   Size:        322      81     168     108       9    1271

  Fragments arranged by size: 

                1271     322     168     108      81       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     117     721     865    1959 
   Size:        117     604     144    1094

  Fragments arranged by size: 

                1094     604     144     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526     577     715    1959 
   Size:        526      51     138    1244

  Fragments arranged by size: 

                1244     526     138      51
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1073    1372    1959 
   Size:       1073     299     587

  Fragments arranged by size: 

                1073     587     299
 
BsaAI yAC'GTr

Cuts at:      0    1239    1959 
   Size:       1239     720
 
BsaWI w'CCGG_w

Cuts at:      0    1762    1959 
   Size:       1762     197
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     124     154    1021    1051    1740    1770    1959 
   Size:        124      30     867      30     689      30     189

  Fragments arranged by size: 

                 867     689     189     124      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     676    1959 
   Size:        676    1283
 
BseYI C'CCAG_C

Cuts at:      0    1725    1959 
   Size:       1725     234
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     174    1864    1959 
   Size:        116      58    1690      95

  Fragments arranged by size: 

                1690     116      95      58
 
BsiHKAI G_wGCw'C

Cuts at:      0      29     184     455     542     727    1051    1175    1327 
   Size:         29     155     271      87     185     324     124     152

Cuts at:   1327    1481    1959 
   Size:        154     478

  Fragments arranged by size: 

                 478     324     271     185     155     154     152     124
                  87      29
 
BsmI GAATG_Cn'

Cuts at:      0      48     783    1959 
   Size:         48     735    1176

  Fragments arranged by size: 

                1176     735      48
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     128     184     455     512     542     727    1051 
   Size:         29      99      56     271      57      30     185     324

Cuts at:   1051    1175    1327    1481    1502    1848    1959 
   Size:        124     152     154      21     346     111

  Fragments arranged by size: 

                 346     324     271     185     154     152     124     111
                  99      57      56      30      29      21
 
BspHI T'CATG_A

Cuts at:      0     746    1565    1803    1959 
   Size:        746     819     238     156

  Fragments arranged by size: 

                 819     746     238     156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       6     979    1497    1959 
   Size:          6     973     518     462

  Fragments arranged by size: 

                 973     518     462       6
 
BsrBI CCG'CTC

Cuts at:      0     774    1959 
   Size:        774    1185
 
BsrFI r'CCGG_y

Cuts at:      0    1452    1959 
   Size:       1452     507
 
BsrGI T'GTAC_A

Cuts at:      0    1262    1959 
   Size:       1262     697
 
BssSI C'ACGA_G

Cuts at:      0     150    1018    1959 
   Size:        150     868     941

  Fragments arranged by size: 

                 941     868     150
 
BstEII G'GTnAC_C

Cuts at:      0    1275    1444    1959 
   Size:       1275     169     515

  Fragments arranged by size: 

                1275     515     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1509    1959 
   Size:       1509     450
 
BstYI r'GATC_y

Cuts at:      0    1199    1388    1959 
   Size:       1199     189     571

  Fragments arranged by size: 

                1199     571     189
 
Bsu36I CC'TnA_GG

Cuts at:      0    1397    1959 
   Size:       1397     562
 
BtgI C'CryG_G

Cuts at:      0     714    1382    1472    1959 
   Size:        714     668      90     487

  Fragments arranged by size: 

                 714     668     487      90
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      87    1959 
   Size:         87    1872
 
BtsI GCAGTG_nn'

Cuts at:      0    1610    1959 
   Size:       1610     349
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     883     918    1959 
   Size:        883      35    1041

  Fragments arranged by size: 

                1041     883      35
 
EaeI y'GGCC_r

Cuts at:      0     365     978    1959 
   Size:        365     613     981

  Fragments arranged by size: 

                 981     613     365
 
EarI CTCTTCn'nnn_

Cuts at:      0     840    1959 
   Size:        840    1119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     274    1959 
   Size:        274    1685
 
EcoICRI GAG'CTC

Cuts at:      0     453     540     725    1959 
   Size:        453      87     185    1234

  Fragments arranged by size: 

                1234     453     185      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     403     571     679     688    1959 
   Size:        322      81     168     108       9    1271

  Fragments arranged by size: 

                1271     322     168     108      81       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     199    1021    1959 
   Size:        199     822     938

  Fragments arranged by size: 

                 938     822     199
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125     192     204     306     350     422    1844    1959 
   Size:        125      67      12     102      44      72    1422     115

  Fragments arranged by size: 

                1422     125     115     102      72      67      44      12
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1021    1053    1959 
   Size:       1021      32     906

  Fragments arranged by size: 

                1021     906      32
 
KpnI G_GTAC'C

Cuts at:      0     237     873    1959 
   Size:        237     636    1086

  Fragments arranged by size: 

                1086     636     237
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1081    1102    1322    1370    1959 
   Size:       1081      21     220      48     589

  Fragments arranged by size: 

                1081     589     220      48      21
 
MscI TGG'CCA

Cuts at:      0     367    1959 
   Size:        367    1592
 
MslI CAynn'nnrTG

Cuts at:      0     262    1000    1258    1270    1959 
   Size:        262     738     258      12     689

  Fragments arranged by size: 

                 738     689     262     258      12
 
MspA1I CmG'CkG

Cuts at:      0     864     983    1668    1959 
   Size:        864     119     685     291

  Fragments arranged by size: 

                 864     685     291     119
 
NaeI GCC'GGC

Cuts at:      0    1454    1959 
   Size:       1454     505
 
NcoI C'CATG_G

Cuts at:      0     714    1959 
   Size:        714    1245
 
NgoMIV G'CCGG_C

Cuts at:      0    1452    1959 
   Size:       1452     507
 
NheI G'CTAG_C

Cuts at:      0     930    1614    1959 
   Size:        930     684     345

  Fragments arranged by size: 

                 930     684     345
 
NsiI A_TGCA'T

Cuts at:      0    1273    1959 
   Size:       1273     686
 
NspI r_CATG'y

Cuts at:      0     615     633    1005    1271    1959 
   Size:        615      18     372     266     688

  Fragments arranged by size: 

                 688     615     372     266      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     300    1840    1959 
   Size:        300    1540     119

  Fragments arranged by size: 

                1540     300     119
 
PmlI CAC'GTG

Cuts at:      0    1239    1959 
   Size:       1239     720
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     124     156     568     600    1959 
   Size:        124      32     412      32    1359

  Fragments arranged by size: 

                1359     412     124      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     204     306    1959 
   Size:        204     102    1653

  Fragments arranged by size: 

                1653     204     102
 
PspOMI G'GGCC_C

Cuts at:      0     124    1844    1959 
   Size:        124    1720     115

  Fragments arranged by size: 

                1720     124     115
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     217     249    1959 
   Size:        217      32    1710

  Fragments arranged by size: 

                1710     217      32
 
PstI C_TGCA'G

Cuts at:      0     314    1959 
   Size:        314    1645
 
SacI G_AGCT'C

Cuts at:      0     455     542     727    1959 
   Size:        455      87     185    1232

  Fragments arranged by size: 

                1232     455     185      87
 
SexAI A'CCwGG_T

Cuts at:      0     872    1959 
   Size:        872    1087
 
SfcI C'TryA_G

Cuts at:      0     310    1350    1959 
   Size:        310    1040     609

  Fragments arranged by size: 

                1040     609     310
 
SmlI C'TyrA_G

Cuts at:      0     541     592     730    1076    1114    1959 
   Size:        541      51     138     346      38     845

  Fragments arranged by size: 

                 845     541     346     138      51      38
 
StuI AGG'CCT

Cuts at:      0     702    1036    1959 
   Size:        702     334     923

  Fragments arranged by size: 

                 923     702     334
 
StyI C'CwwG_G

Cuts at:      0     714     901    1195    1931    1959 
   Size:        714     187     294     736      28

  Fragments arranged by size: 

                 736     714     294     187      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1208    1959 
   Size:       1208     751
 
TatI w'GTAC_w

Cuts at:      0    1262    1415    1672    1959 
   Size:       1262     153     257     287

  Fragments arranged by size: 

                1262     287     257     153
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      78     110    1959 
   Size:         78      32    1849

  Fragments arranged by size: 

                1849      78      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1371    1959 
   Size:       1371     588
 
XhoI C'TCGA_G

Cuts at:      0    1076    1959 
   Size:       1076     883
 
XmnI GAAnn'nnTTC

Cuts at:      0     685    1959 
   Size:        685    1274
 

 Enzymes that do cut:

     AarI     AccI   Acc65I    AflII     AleI     AloI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NheI     NsiI     NspI    PflMI     PmlI     PpiI    PpuMI
   PspOMI     PsrI     PstI     SacI    SexAI     SfcI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI   AflIII     AgeI     AhdI     AlfI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI     BclI     BglI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspEI    BsrDI   BssHII   BstAPI    BstBI  BstZ17I     ClaI
     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NarI     NdeI     NotI     NruI     PacI     PasI     PciI
     PfoI     PmeI    PshAI     PsiI    PspXI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XmaI     ZraI
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