Restriction map for pF1KB5034
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BseAI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeIII      MamI        MfeI        MluI        MroI        
MslI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TfiI        Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI           31  GTAGAC               GT^[AC][GT]AC
ApaI           59  GGGCCC               GGGCC^C
BamHI         410  GGATCC               G^GATCC
BanI          823  GGTGCC               G^G[CT][GA]CC
BbsI          639  GAAGAC               GAAGAC(2/6)
BbvCI         870  GCTGAGG              CCTCAGC(-5/-2)
BpuAI         639  GAAGAC               GAAGAC(2/6)
BsaAI         400  TACGTG               [CT]AC^GT[GA]
BsaI          739  GAGACC               GGTCTC(1/5)
BsaWI         311  ACCGGA               [AT]^CCGG[AT]
BsiEI         135  CGACCG               CG[GA][CT]^CG
Bsp1407I      762  TGTACA               T^GTACA
BspMI         369  GCAGGT               ACCTGC(4/8)
BsrBI         406  GAGCGG               CCGCTC(-3/-3)
BsrFI         718  GCCGGC               [GA]^CCGG[CT]
BsrGI         762  TGTACA               T^GTACA
BstXI         329  CCAAGGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        718  GCCGGC               [GA]^CCGG[CT]
EcoT22I       455  ATGCAT               ATGCA^T
FauI          464  CCCGC                CCCGC(4/6)
HaeII         321  GGCGCT               [GA]GCGC^[CT]
HphI          352  GGTGA                GGTGA(8/7)
MaeII         401  ACGT                 A^CGT
NaeI          718  GCCGGC               GCC^GGC
NcoI          299  CCATGG               C^CATGG
NgoMIV        718  GCCGGC               G^CCGGC
NsiI          455  ATGCAT               ATGCA^T
PaeR7I        241  CTCGAG               C^TCGAG
PpuMI         547  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         840  CAGCTG               CAG^CTG
SacI          103  GAGCTC               GAGCT^C
SmlI          241  CTCGAG               C^T[CT][GA]AG
SspBI         762  TGTACA               T^GTACA
TaqI          242  TCGA                 T^CGA
TthHB8I       242  TCGA                 T^CGA
XcmI          734  CCAGAGAGACCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          241  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwNI         493  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              837  CAGCAGCTG           
AspHI         103  GAGCTC               G[AT]GC[AT]^C
              518  GAGCAC              
AvaI          241  CTCGAG               C^[CT]CG[GA]G
              483  CTCGGG              
BalI          206  TGGCCA               TGG^CCA
              731  TGGCCA              
BanII          59  GGGCCC               G[GA]GC[CT]^C
              103  GAGCTC              
BcnI          138  CCGGG                CC^[GC]GG
              606  CCGGG               
BfaI           64  CTAG                 C^TAG
              916  CTAG                
BglI          208  GCCACCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              667  GCCAACAAGGC         
BpmI          886  CTCCAG               CTGGAG(16/14)
              829  CTGGAG              
BsgI          307  CTGCAC               GTGCAG(16/14)
              373  GTGCAG              
BsiHKAI       103  GAGCTC               G[AT]GC[AT]^C
              518  GAGCAC              
BsmAI         739  GAGAC                GTCTC(1/5)
              779  GAGAC               
BsoBI         241  CTCGAG               C^[CT]CG[GA]G
              483  CTCGGG              
BstYI         410  GGATCC               [GA]^GATC[CT]
              477  GGATCT              
CfoI          258  GCGC                 GCG^C
              322  GCGC                
DsaI          212  CCACGG               C^C[GA][CT]GG
              299  CCATGG              
EcoNI          63  CCTAGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              240  CCTCGAGGAGG         
HhaI          258  GCGC                 GCG^C
              322  GCGC                
HinP1I        258  GCGC                 G^CGC
              322  GCGC                
HinfI          37  GAGTC                G^A[ACGT]TC
              926  GACTC               
MaeI           64  CTAG                 C^TAG
              916  CTAG                
MboII         501  GAAGA                GAAGA(8/7)
              639  GAAGA               
MflI          410  GGATCC               [GA]^GATC[CT]
              477  GGATCT              
MluNI         206  TGGCCA               TGG^CCA
              731  TGGCCA              
MscI          206  TGGCCA               TGG^CCA
              731  TGGCCA              
MseI          185  TTAA                 T^TAA
              200  TTAA                
NciI          138  CCGGG                CC^[GC]GG
              606  CCGGG               
NspI          689  ACATGC               [GA]CATG^[CT]
              782  ACATGC              
PleI          926  GACTC                GAGTC(4/5)
               37  GAGTC               
Tru9I         185  TTAA                 T^TAA
              200  TTAA                
XhoII         410  GGATCC               [GA]^GATC[CT]
              477  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MboI        MnlI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
StyI        TseI        TspRI       
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