Restriction map for pF1KB5034
 (Linear) (Six-base) MAPSORT of: KIBB5034.seq  Check: 5669  from: 1  to: 948



KIBB5034.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     360     948 
   Size:        360     588
 
AccI GT'mk_AC

Cuts at:      0      32     948 
   Size:         32     916
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     520     948 
   Size:        130     390     428

  Fragments arranged by size: 

                 428     390     130
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     739     771     948 
   Size:        739      32     177

  Fragments arranged by size: 

                 739     177      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     498     842     948 
   Size:        498     344     106

  Fragments arranged by size: 

                 498     344     106
 
ApaI G_GGCC'C

Cuts at:      0      63     948 
   Size:         63     885
 
AvaI C'yCGr_G

Cuts at:      0     241     483     948 
   Size:        241     242     465

  Fragments arranged by size: 

                 465     242     241
 
BamHI G'GATC_C

Cuts at:      0     410     948 
   Size:        410     538
 
BanI G'GyrC_C

Cuts at:      0     823     948 
   Size:        823     125
 
BanII G_rGCy'C

Cuts at:      0      63     107     948 
   Size:         63      44     841

  Fragments arranged by size: 

                 841      63      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     646     948 
   Size:        646     302
 
BbvCI CC'TCA_GC

Cuts at:      0     871     948 
   Size:        871      77
 
BfrBI ATG'CAT

Cuts at:      0     457     948 
   Size:        457     491
 
BglI GCCn_nnn'nGGC

Cuts at:      0     214     673     948 
   Size:        214     459     275

  Fragments arranged by size: 

                 459     275     214
 
Bme1580I G_kGCm'C

Cuts at:      0      63     382     828     948 
   Size:         63     319     446     120

  Fragments arranged by size: 

                 446     319     120      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     147     369     546     948 
   Size:        147     222     177     402

  Fragments arranged by size: 

                 402     222     177     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     850     871     948 
   Size:        850      21      77

  Fragments arranged by size: 

                 850      77      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     871     948 
   Size:        871      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     733     948 
   Size:        733     215
 
BsaAI yAC'GTr

Cuts at:      0     402     948 
   Size:        402     546
 
BsaWI w'CCGG_w

Cuts at:      0     311     948 
   Size:        311     637
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     512     542     739     769     948 
   Size:        512      30     197      30     179

  Fragments arranged by size: 

                 512     197     179      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     259     379     676     948 
   Size:        259     120     297     272

  Fragments arranged by size: 

                 297     272     259     120
 
BseYI C'CCAG_C

Cuts at:      0     512     532     584     880     948 
   Size:        512      20      52     296      68

  Fragments arranged by size: 

                 512     296      68      52      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     394     948 
   Size:        292     102     554

  Fragments arranged by size: 

                 554     292     102
 
BsiEI CG_ry'CG

Cuts at:      0     138     948 
   Size:        138     810
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     522     948 
   Size:        107     415     426

  Fragments arranged by size: 

                 426     415     107
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     107     382     522     828     948 
   Size:         63      44     275     140     306     120

  Fragments arranged by size: 

                 306     275     140     120      63      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     360     948 
   Size:        360     588
 
BsrBI CCG'CTC

Cuts at:      0     408     948 
   Size:        408     540
 
BsrFI r'CCGG_y

Cuts at:      0     718     948 
   Size:        718     230
 
BsrGI T'GTAC_A

Cuts at:      0     762     948 
   Size:        762     186
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     948 
   Size:        336     612
 
BstYI r'GATC_y

Cuts at:      0     410     477     948 
   Size:        410      67     471

  Fragments arranged by size: 

                 471     410      67
 
BtgI C'CryG_G

Cuts at:      0     212     299     948 
   Size:        212      87     649

  Fragments arranged by size: 

                 649     212      87
 
BtsI GCAGTG_nn'

Cuts at:      0     382     948 
   Size:        382     566
 
EaeI y'GGCC_r

Cuts at:      0     206     731     899     948 
   Size:        206     525     168      49

  Fragments arranged by size: 

                 525     206     168      49
 
EcoICRI GAG'CTC

Cuts at:      0     105     948 
   Size:        105     843
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     520     850     871     948 
   Size:        130     390     330      21      77

  Fragments arranged by size: 

                 390     330     130      77      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      67     244     948 
   Size:         67     177     704

  Fragments arranged by size: 

                 704     177      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59      60     548     948 
   Size:         59       1     488     400

  Fragments arranged by size: 

                 488     400      59       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     186     948 
   Size:        186     762
 
HaeII r_GCGC'y

Cuts at:      0     325     948 
   Size:        325     623
 
MscI TGG'CCA

Cuts at:      0     208     733     948 
   Size:        208     525     215

  Fragments arranged by size: 

                 525     215     208
 
MspA1I CmG'CkG

Cuts at:      0       7     842     904     948 
   Size:          7     835      62      44

  Fragments arranged by size: 

                 835      62      44       7
 
NaeI GCC'GGC

Cuts at:      0     720     948 
   Size:        720     228
 
NcoI C'CATG_G

Cuts at:      0     299     948 
   Size:        299     649
 
NgoMIV G'CCGG_C

Cuts at:      0     718     948 
   Size:        718     230
 
NsiI A_TGCA'T

Cuts at:      0     459     948 
   Size:        459     489
 
NspI r_CATG'y

Cuts at:      0     693     786     948 
   Size:        693      93     162

  Fragments arranged by size: 

                 693     162      93
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     739     771     948 
   Size:        739      32     177

  Fragments arranged by size: 

                 739     177      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     548     948 
   Size:        548     400
 
PspOMI G'GGCC_C

Cuts at:      0      59     948 
   Size:         59     889
 
PspXI vC'TCGA_Gb

Cuts at:      0     241     948 
   Size:        241     707
 
PvuII CAG'CTG

Cuts at:      0     842     948 
   Size:        842     106
 
SacI G_AGCT'C

Cuts at:      0     107     948 
   Size:        107     841
 
SanDI GG'GwC_CC

Cuts at:      0     548     948 
   Size:        548     400
 
SmlI C'TyrA_G

Cuts at:      0     241     948 
   Size:        241     707
 
StyI C'CwwG_G

Cuts at:      0     122     299     329     383     743     948 
   Size:        122     177      30      54     360     205

  Fragments arranged by size: 

                 360     205     177     122      54      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166     948 
   Size:        166     782
 
TatI w'GTAC_w

Cuts at:      0     290     762     948 
   Size:        290     472     186

  Fragments arranged by size: 

                 472     290     186
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     512     544     948 
   Size:        512      32     404

  Fragments arranged by size: 

                 512     404      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     741     948 
   Size:        741     207
 
XhoI C'TCGA_G

Cuts at:      0     241     948 
   Size:        241     707
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AloI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BfrBI     BglI Bme1580I     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
    BstXI    BstYI     BtgI     BtsI     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII     MscI   MspA1I     NaeI     NcoI   NgoMIV
     NsiI     NspI     PpiI    PpuMI   PspOMI    PspXI    PvuII     SacI
    SanDI     SmlI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BglII     BlpI    BmgBI
     BmtI     BplI    BpuEI    BsaBI    BsaHI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NarI     NdeI     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI
     PsrI     PstI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XmaI     XmnI     ZraI
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