Restriction map for
pF1KB3324
(Linear) (Six-base) MAPSORT of: KIBB3324.seq Check: 9124 from: 1 to: 1815
KIBB3324.orf
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 0 1295 1641 1815
Size: 1295 346 174
Fragments arranged by size:
1295 346 174
Acc65I G'GTAC_C
Cuts at: 0 644 1815
Size: 644 1171
AflIII A'CryG_T
Cuts at: 0 1130 1815
Size: 1130 685
AhdI GACnn_n'nnGTC
Cuts at: 0 1637 1815
Size: 1637 178
AleI CACnn'nnGTG
Cuts at: 0 1145 1815
Size: 1145 670
AlwNI CAG_nnn'CTG
Cuts at: 0 133 257 1815
Size: 133 124 1558
Fragments arranged by size:
1558 133 124
ApoI r'AATT_y
Cuts at: 0 156 219 451 884 1010 1369 1693 1815
Size: 156 63 232 433 126 359 324 122
Fragments arranged by size:
433 359 324 232 156 126 122 63
AvaI C'yCGr_G
Cuts at: 0 557 1815
Size: 557 1258
AvrII C'CTAG_G
Cuts at: 0 196 1815
Size: 196 1619
BanI G'GyrC_C
Cuts at: 0 644 1815
Size: 644 1171
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 307 873 1815
Size: 179 128 566 942
Fragments arranged by size:
942 566 179 128
BclI T'GATC_A
Cuts at: 0 936 1064 1815
Size: 936 128 751
Fragments arranged by size:
936 751 128
BglII A'GATC_T
Cuts at: 0 977 1815
Size: 977 838
Bme1580I G_kGCm'C
Cuts at: 0 362 500 1815
Size: 362 138 1315
Fragments arranged by size:
1315 362 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1030 1794 1815
Size: 66 964 764 21
Fragments arranged by size:
964 764 66 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1815
Size: 22 1793
BsaI GGTCTCn'nnnn_
Cuts at: 0 68 1815
Size: 68 1747
BsaWI w'CCGG_w
Cuts at: 0 1253 1663 1815
Size: 1253 410 152
Fragments arranged by size:
1253 410 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 776 1815
Size: 776 1039
BseYI C'CCAG_C
Cuts at: 0 72 1815
Size: 72 1743
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1815
Size: 1144 671
BsiEI CG_ry'CG
Cuts at: 0 11 1815
Size: 11 1804
BsiHKAI G_wGCw'C
Cuts at: 0 52 810 1623 1815
Size: 52 758 813 192
Fragments arranged by size:
813 758 192 52
BsmI GAATG_Cn'
Cuts at: 0 786 1006 1659 1815
Size: 786 220 653 156
Fragments arranged by size:
786 653 220 156
Bsp1286I G_dGCh'C
Cuts at: 0 52 362 500 810 1623 1815
Size: 52 310 138 310 813 192
Fragments arranged by size:
813 310 310 192 138 52
BspEI T'CCGG_A
Cuts at: 0 1253 1663 1815
Size: 1253 410 152
Fragments arranged by size:
1253 410 152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1201 1815
Size: 1201 614
BsrDI GCAATG_nn'
Cuts at: 0 1211 1403 1815
Size: 1211 192 412
Fragments arranged by size:
1211 412 192
BstAPI GCAn_nnn'nTGC
Cuts at: 0 292 1815
Size: 292 1523
BstEII G'GTnAC_C
Cuts at: 0 1188 1815
Size: 1188 627
BstYI r'GATC_y
Cuts at: 0 977 1593 1815
Size: 977 616 222
Fragments arranged by size:
977 616 222
BtgI C'CryG_G
Cuts at: 0 1088 1815
Size: 1088 727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 1815
Size: 126 1689
BtsI GCAGTG_nn'
Cuts at: 0 290 433 888 1815
Size: 290 143 455 927
Fragments arranged by size:
927 455 290 143
DraIII CAC_nnn'GTG
Cuts at: 0 616 1815
Size: 616 1199
EaeI y'GGCC_r
Cuts at: 0 8 317 1815
Size: 8 309 1498
Fragments arranged by size:
1498 309 8
EagI C'GGCC_G
Cuts at: 0 8 1815
Size: 8 1807
EarI CTCTTCn'nnn_
Cuts at: 0 729 1662 1815
Size: 729 933 153
Fragments arranged by size:
933 729 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 1815
Size: 106 1709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1030 1794 1815
Size: 66 964 764 21
Fragments arranged by size:
964 764 66 21
EcoO109I rG'GnC_Cy
Cuts at: 0 1379 1565 1815
Size: 1379 186 250
Fragments arranged by size:
1379 250 186
EcoRI G'AATT_C
Cuts at: 0 156 219 884 1010 1693 1815
Size: 156 63 665 126 683 122
Fragments arranged by size:
683 665 156 126 122 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 592 624 693 725 1216 1248
Size: 415 32 145 32 69 32 491 32
Cuts at: 1248 1268 1300 1623 1655 1815
Size: 20 32 323 32 160
Fragments arranged by size:
491 415 323 160 145 69 32 32
32 32 32 32 20
HindIII A'AGCT_T
Cuts at: 0 679 1815
Size: 679 1136
KpnI G_GTAC'C
Cuts at: 0 648 1815
Size: 648 1167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 1815
Size: 1248 567
MscI TGG'CCA
Cuts at: 0 319 1815
Size: 319 1496
MslI CAynn'nnrTG
Cuts at: 0 295 999 1145 1186 1815
Size: 295 704 146 41 629
Fragments arranged by size:
704 629 295 146 41
MspA1I CmG'CkG
Cuts at: 0 245 1759 1815
Size: 245 1514 56
Fragments arranged by size:
1514 245 56
NspI r_CATG'y
Cuts at: 0 102 669 1493 1815
Size: 102 567 824 322
Fragments arranged by size:
824 567 322 102
PasI CC'CwG_GG
Cuts at: 0 127 1815
Size: 127 1688
PflMI CCAn_nnn'nTGG
Cuts at: 0 133 1815
Size: 133 1682
PpuMI rG'GwC_Cy
Cuts at: 0 1379 1815
Size: 1379 436
PstI C_TGCA'G
Cuts at: 0 1629 1815
Size: 1629 186
PvuII CAG'CTG
Cuts at: 0 245 1759 1815
Size: 245 1514 56
Fragments arranged by size:
1514 245 56
SbfI CC_TGCA'GG
Cuts at: 0 1629 1815
Size: 1629 186
ScaI AGT'ACT
Cuts at: 0 1362 1815
Size: 1362 453
SfcI C'TryA_G
Cuts at: 0 1625 1681 1815
Size: 1625 56 134
Fragments arranged by size:
1625 134 56
SmlI C'TyrA_G
Cuts at: 0 37 1815
Size: 37 1778
SphI G_CATG'C
Cuts at: 0 102 669 1815
Size: 102 567 1146
Fragments arranged by size:
1146 567 102
StuI AGG'CCT
Cuts at: 0 722 1815
Size: 722 1093
StyI C'CwwG_G
Cuts at: 0 196 1815
Size: 196 1619
TatI w'GTAC_w
Cuts at: 0 1310 1360 1418 1815
Size: 1310 50 58 397
Fragments arranged by size:
1310 397 58 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1545 1577 1815
Size: 1545 32 238
Fragments arranged by size:
1545 238 32
XmnI GAAnn'nnTTC
Cuts at: 0 1154 1815
Size: 1154 661
Enzymes that do cut:
AccI Acc65I AflIII AhdI AleI AlwNI ApoI AvaI
AvrII BanI BbsI BclI BglII Bme1580I BpmI BpuEI
BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BstAPI BstEII BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NspI PasI PflMI PpuMI PstI PvuII SbfI
ScaI SfcI SmlI SphI StuI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaXI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI