Restriction map for pF1KE1073
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BglI        BglII       BlnI        BpuAI       
BsaBI       BsaHI       BsaI        BseAI       BsiWI       
BsmBI       Bsp1407I    BspEI       BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     Cfr10I      CpoI        DraII       DrdI        
Eam1105I    Eco47III    EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       FseI        FspI        HaeII       
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AflII        1774  CTTAAG               C^TTAAG
Alw44I       1412  GTGCAC               G^TGCAC
AlwNI        1814  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          771  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1412  GTGCAC               G^TGCAC
Asp700I       809  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1688  CCCGAG               C^[CT]CG[GA]G
AvaII        1884  GGACC                G^G[AT]CC
BamHI        2116  GGATCC               G^GATCC
BclI          180  TGATCA               T^GATCA
BfrI         1774  CTTAAG               C^TTAAG
BlpI         1789  GCTGAGC              GC^T[ACGT]AGC
BpmI         1066  CTCCAG               CTGGAG(16/14)
Bpu1102I     1789  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2404  TACGTG               [CT]AC^GT[GA]
BsaWI         295  TCCGGT               [AT]^CCGG[AT]
BseRI        1639  GAGGAG               GAGGAG(10/8)
BsiEI          90  CGGCCG               CG[GA][CT]^CG
BsmAI         751  GAGAC                GTCTC(1/5)
BsoBI        1688  CCCGAG               C^[CT]CG[GA]G
BspDI        1618  ATCGAT               AT^CGAT
BsrDI        1752  CATTGC               GCAATG(2/0)
BstAPI       1723  GCAAAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1454  TTCGAA               TT^CGAA
BstYI        2116  GGATCC               [GA]^GATC[CT]
Bsu36I        771  CCTGAGG              CC^T[ACGT]AGG
CelII        1789  GCTGAGC              GC^T[ACGT]AGC
CfoI          118  GCGC                 GCG^C
ClaI         1618  ATCGAT               AT^CGAT
DraIII        278  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          351  CCATGG               C^C[GA][CT]GG
EagI           90  CGGCCG               C^GGCCG
EclXI          90  CGGCCG               C^GGCCG
Eco52I         90  CGGCCG               C^GGCCG
Eco81I        771  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      1949  ATGCAT               ATGCA^T
FbaI          180  TGATCA               T^GATCA
HhaI          118  GCGC                 GCG^C
HinP1I        118  GCGC                 G^CGC
MfeI         1600  CAATTG               C^AATTG
MflI         2116  GGATCC               [GA]^GATC[CT]
MslI          742  CATAACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1600  CAATTG               C^AATTG
NcoI          351  CCATGG               C^CATGG
NheI         1482  GCTAGC               G^CTAGC
NsiI         1949  ATGCAT               ATGCA^T
NspI          676  GCATGC               [GA]CATG^[CT]
NspV         1454  TTCGAA               TT^CGAA
PvuII        2147  CAGCTG               CAG^CTG
SalI          110  GTCGAC               G^TCGAC
SapI         1768  GAAGAGC              GCTCTTC(1/4)
ScaI         1663  AGTACT               AGT^ACT
SfuI         1454  TTCGAA               TT^CGAA
SphI          676  GCATGC               GCATG^C
StuI          521  AGGCCT               AGG^CCT
XhoII        2116  GGATCC               [GA]^GATC[CT]
XmnI          809  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          287  AAATTC               [GA]^AATT[CT]
             1697  AAATTT              
AlwI         2117  GATCC                GGATC(4/5)
             2116  GGATC               
ApoI          287  AAATTC               [GA]^AATT[CT]
             1697  AAATTT              
BalI           14  TGGCCA               TGG^CCA
             2417  TGGCCA              
BcnI          404  CCCGG                CC^[GC]GG
             1635  CCGGG               
BsgI         1164  CTGCAC               GTGCAG(16/14)
             2197  GTGCAG              
DraI         1392  TTTAAA               TTT^AAA
             2154  TTTAAA              
EarI         2162  CTCTTC               CTCTTC(1/4)
             1768  GAAGAG              
EcoT14I        11  CCTTGG               C^C[AT][AT]GG
              351  CCATGG              
HgaI           57  GCGTC                GACGC(5/10)
             1816  GCGTC               
HindIII      1082  AAGCTT               A^AGCTT
             1246  AAGCTT              
Ksp632I      2162  CTCTTC               CTCTTC(1/4)
             1768  GAAGAG              
MaeII         323  ACGT                 A^CGT
             2405  ACGT                
MluNI          14  TGGCCA               TGG^CCA
             2417  TGGCCA              
MscI           14  TGGCCA               TGG^CCA
             2417  TGGCCA              
NciI          404  CCCGG                CC^[GC]GG
             1635  CCGGG               
SacI         1063  GAGCTC               GAGCT^C
             2290  GAGCTC              
SfcI          514  CTACAG               C^T[GA][CT]AG
             1334  CTACAG              
StyI           11  CCTTGG               C^C[AT][AT]GG
              351  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AspHI       
BanII       BbvI        BfaI        BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmFI       BsmI        
Bsp1286I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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